• Title/Summary/Keyword: mMT

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Revealing the complete mtDNA genome sequence of Cemani chicken (Gallus gallus) by using Nanopore sequencing analysis

  • Sutopo Sutopo;Dela Ayu Lestari;Asep Setiaji;Sri Rachma Aprilita Bugiwati;Muhammad Ihsan Andi Dagong;Nena Hilmia;Dani Garnida;Indrawati Yudha Asmara;Edy Kurnianto
    • Animal Bioscience
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    • v.37 no.10
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    • pp.1664-1672
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    • 2024
  • Objective: This study aimed to identify, discover and explore the characteristics of the mtDNA genomes of Cemani chicken (Gallus gallus). Methods: This study used gDNA of Cemani chicken isolated from liver tissue. mtDNA sequencing was performed using WGS mtDNA analysis with nanopore technology by Oxford Nanopore Technologies GridION. Bioinformatics and data analysis were then performed. Results: This study showed that the length of the mtDNA genome is 16,789 bp, consisting of two ribosomal RNA (12S rRNA, 16S rRNA), 22 transfer RNA genes (trnR, trnG, trnK, trnD, trnS, trnY, trnC, trnN, trnA, trnW, trnM, trnQ, trnl, trnL, trnV, trnF, trnP, trnT, trnE, trnL, trnS, trnH), 13 protein-coding genes (PCGs) (ND4l, ND3, COX3, ATP6, ATP8, COX2, COX1, ND2, ND1, CYTB, ND6, ND5, ND4), and a noncoding control region (Dloop). Furthermore, analysis showed there were polymorphic sites and amino acid alterations when mtDNA Cemani chicken was aligned with references from GenBank. Conclusion: Site (988T>*) in Dloop genes and (328A>G) in ND3 genes which alter glycine to stop codon, were specific markers found only in Cemani chicken.

Phytosociological Study on the Vegetation of Mt. Mudeung (無等山의 植生에 對한 植物社會學的 硏究)

  • Kim, Chul-Soo;Jang-Geun Oh
    • The Korean Journal of Ecology
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    • v.16 no.1
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    • pp.93-114
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    • 1993
  • The vegetation of Mt. Mudeung was investigated from April, 1991 to September, 1992. The units of vegetation were classified 10 units by the Braun-Blanquet's phytosociological method. The forest vegetation was classified into 10 communities, Pinus densiflora, Pinus vigida, Chamaecyparis obtusa afforestation, Quercus mongolica, Q. variabilis, Q. serrata, Q. acutissima, Miscanthus sinensis var. purpurascens, Hylomecon hylomeconoides and Drosera rotundifolia community. Based on the classification, the actual vegetation map and degree of green naturality were drawn in 1:50,000 scale. The vertical distribution of the main component species was investigated based on the vegetation data of the EN slope and SW slope of Mt. Mudeung from altitude 200m to top.

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Analysis of Mitochondrial DNA Mutation in hepatoma

  • Chung, Ku-Sun;Lee, Kyo-Young;Shim, Sang-In;Kim, Jin-Sun;Song, Eun-Sook
    • BMB Reports
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    • v.33 no.5
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    • pp.417-421
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    • 2000
  • Mitochondrial DNA (mtDNA) mutation was investigated in a hepatoma patient using a polymerase chain reaction (PCR) and an in situ hybridization technique. Biotin-labeled probes for the subunit m of cytochrome c oxidase revealed differences in the in situ hybridization. A PCR assay using biopsied and microdissected tissues showed that common deletion (4,977 bp) was more pronounced in the cancer region than in the normal parts of the same patient. These results suggest that mtDNA deletion might be associated with tumorigenesis in hepatoma.

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Phytosociological Study on the Forest Vegetation in Mt. Komdan (黔丹山 森林植生의 植物社會學的 硏究)

  • Lee, Ho-Joon;Ha-Song Kim;Eun-Bu Cho
    • The Korean Journal of Ecology
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    • v.14 no.3
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    • pp.273-303
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    • 1991
  • A phytosociological study of forest vegetation was carried out in mt. komdan in the period from October 7,1985 to October 15 1989. The forst vegetation could be classified into 7 communities ; quercus monbolica,pinus densiflora, quercus dentata, pinus koraiensis, larix leptoletsis, pinus rigida,castanea creaneta. The principal forest community in mt. komdan-the quercus mongulica community- was distributed mainly at the altitude of 400~600m and the importance value was 187.1. The pinus desiflora community was 137.9. since pinus koraiensis, larix laptolepsis,pinus rigida, castanea creanata, etc. werw planted in this region, It seems that it was frequently disturbed by human interference,especilly, the destruction of the vegetation in the recreation ground of paldang, the regions around the village of hasangok-ri and the main routes for mountain climbing appeared severely.

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Biogeography of the Alpine Plants at Hallasan, Jeju Island, Korea

  • Kong, Woo-Seok
    • The Korean Journal of Quaternary Research
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    • v.19 no.2
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    • pp.40-43
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    • 2005
  • The island alpine biogeography of Mt. Halla (Hallasan), Jeju Island (Jejudo), Korea is discussed. The presence of numerous species of alpine flora on Mt. Halla, the southernmost distributional limit for certain species, may primarily be attributed to palaeo-environmental factors, since it can not be wholly explained by reference to current environmental conditions. The alpine flora on the peak of Mt. Halla, mainly above 1,500m a.s.l, is evidently descended from immigrants from NE Asia via the Korean Peninsula during the epochs of the Ice Age. These plants, which are very intolerant of competition with temperate vegetation, have been able to persist in alpine belts thanks to their harsh climatic conditions, sterile soil, rugged topography and cryoturbation. The alpine plants on Hallasan are in a stage or process of retreat toward the mountaintop, most likely due to recent climatic amelioration. The lower limit of some species seems to coincide with maximum summer isotherms. The continued survival of arctic-alpine and alpine plants on the summit of Hallasan, Jejudo, the Korean Peninsula, however, is in danger, if global warming associated with the greenhouse effect continues.

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Flora of Mt. Woosanbong, Korea

  • Kang, Shin-Ho;Lee, Young-Sim;Ko, Sung-Chul
    • Plant Resources
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    • v.6 no.1
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    • pp.57-75
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    • 2003
  • This study was performed to investigate the flora and plant resources of Mt. Woosanbong (537.8 m) from April to October 2002. The collected vascular plants were composed of all 389 taxa including cultivated species, and classified into 329 species, 2 subspecies, 53 varieties, and 5 forms of 248 genera under 81 families. Six taxa of the Korean endemic plants and 4 taxa of the rare and endangered plants were also distributed in this mount. Resource plants were categorized into edible 181, pasturing 160, medicinal 136, stainable 94, ornamental 77, timber 22, fiber 5 and industrial 4 taxa, respectively. Floristic geography of the investigated area was regarded as the boundary between middle and southern parts in floristic pattern of the Korean Peninsula.

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Classification Analysis and Gradient Analysis on the Forest Vegetation of Mt. Mudung (分類法과 傾度分析에 의한 無等山 蒜林植生 分析)

  • Kim, Chang-Hwan;Kang, Seon-Hee;Kil, Bong-Seop
    • The Korean Journal of Ecology
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    • v.17 no.4
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    • pp.471-484
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    • 1994
  • The forest vegetation types and their structural characteristics in Mt. $Mud\v{u}ng$ were investigated by classification method and ordination method. The forest was classified into 7 communities by ristic composition table: Quercus monogolica community, Q. serrata community, Q.acutissima community, Q.variabilis community, Q.dentata community, Pinus densiflora community and Frainus mandshurica community. Considering the moisture gradient, two kinds of distributuin pattern were shown as follows; F. mandshurica, Q. acturissima, Platycarya strobilacea and Staphylea bumalda were distribute at moist habitats, while Q. monogolica, P. densiflora and Q.variabilis at dry habitats. In continuum analysis, each population occupied different distribution area but it was continuously overlapped. On the successional trends of tree species, it is postulated that Q. mongolica species might dominate the altitudinal zone over 700m.

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A new catalogue of galactic eccentric eclipsing binary stars

  • Kim, Chun-Hwey;Kreiner, J.M.;Zakrzewski, B.;Ogloza, W.;Kim, Hyunwoo;Jeong, Min-ji
    • The Bulletin of The Korean Astronomical Society
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    • v.42 no.2
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    • pp.63.2-63.2
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    • 2017
  • 우리 은하에 있는 618개 이심궤도 식쌍성의 카타로그를 제작하였다. 그 이심 식쌍성들은 우리의 극심시각 database에 수록된, 또는 여러 전천 탐사 자료에서 수집된 측광 자료로부터 새롭게 결정된, 또는 우리가 직접 관측하여 얻은 약 13만개의 극심시각 자료의 식시각도 분석을 통하여 편집된 것이다. 618개의 이심 식쌍성 중에서 근성점 운동을 보이는 시스템은 모두 170개이며, 이중에서 30개의 쌍성이 근성점 운동과 광시간 효과가 동시에 일어난다. 근성점 운동을 보이는 별들의 근성점 운동 변수들을 일관된 방식으로 산출하여 카타로그에 수록하였다. 우리가 작성한 최신의 카타로그는 기존 카타로그에 비해 양과 내용에 있어 가장 방대하다. 우리 은하 근성점 운동 쌍성들과 소마제란 성운과 대마젤란 성운에서 발견된 근성점 운동 쌍성들을 모아 근성점 운동 변수들의 통계적 분포를 살펴 보았고, 그 결과들을 논의한다.

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Native Environment and Growth Characteristics of Dendropanax morbifera Lev. In Korea

  • Park, Seong-Kyu;Park, Kyong-Ju;Lee, Jong il
    • Korean Journal of Plant Resources
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    • v.10 no.3
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    • pp.271-273
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    • 1997
  • This study was carried out to obtain basic envirinmental information of the native area and growth characteristics at different age of Dendropanax morbifera LEV. In the southern Korea. The Dendropanax morbifera LEV. Was grown naturally at Wando, Cheju(Mt. Halla), Yecheon(Kermoon island),Sinan(Hong isand), Jindo(Mt. Chermchill) and Haenam(Mt. Taeroon), all between 30 to 450m above the sea level. The soil characterisitics of the native area had a pH of 5.3 to 5.4 with 8.4 to 9.8% content of organic matter and 18.2 to 21.3% soil moisture. Flowering starting in the middle of July begun when the trees were 6 years old. Six year old trees are 129 cm in stem height, 34 mm in stem diameter,15.4 in number of leaves with petioles 12.9 cm long. Xylem sap can be obtained from over 10 year old trees that grew well and have over 10 cm stems in diameter.

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Plant Resources of Mt. Gamak

  • Kang, Shin-Ho
    • Korean Journal of Plant Resources
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    • v.22 no.3
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    • pp.248-265
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    • 2009
  • This study was performed to investigate the flora and plant resources of Mt. Gamak (537.8 m) from April to October 2007. The collected vascular plants were composed of all 478 taxa including cultivated species, and classified into 410 species, 3 subspecies, 61 varieties, and 4 forms of 284 genera under 94 families. Six taxa of the Korean endemic plants and 4 taxa of the rare and endangered plants were also distributed in this mount. Resource plants were categorized into edible 222, pasturing 208, medicinal 183, stainable 134, ornamental 96, timber 20, fiber 6 and industrial 4 taxa, respectively. Floristic geography of the investigated area was regarded as the boundary between middle parts in floristic pattern of the Korean Peninsula.