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Application of Botulinum Toxin on Masticatory Muscle of Patients with Bruxism

  • Jang, Seok-Min;Jeon, Hye-Mi;Kim, Kyung-Hee;Ok, Soo-Min;Heo, Jun-Young;Jeong, Sung-Hee;Ahn, Yong-Woo
    • Journal of Oral Medicine and Pain
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    • v.39 no.2
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    • pp.55-62
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    • 2014
  • Purpose: This study aims to evaluate the changes in soft tissue thickness of the masseteric region after injection of botulinum toxin type A (BTX-A). Methods: Twenty-four data acquired from medical records were classified into 4 groups: bruxer group that received masseter muscle injection only (M-B), bruxer group that received both masseter and temporalis muscle injections (MT-B), non-bruxer group that received masseter muscle injection only (M-NB) and non-bruxer group that received both masseter and temporalis muscle injections (MT-NB). Injection dose of BTX-A was 30 units for each masseter muscle and 20 units for each temporalis muscle. We measured the reduced thickness of the masseteric region before and after 12 weeks after injection using cone-beam computed tomography. Results: Among the patients that received both masseter and temporalis muscle injections, bruxer group showed a tendency to have more reduction in masseter muscle thickness than non-bruxer group. The difference in reduced thickness between M-B and MT-B tended to show greater than the difference between M-NB and MT-NB. Conclusions: In case of masseter hypertrothy patients with bruxism there was a tendency to show a difference in reduced thickness of soft tissue between the group that received both masseter and temporalis muscles injection and the group that received masseter muscle injection only hence a thorough inspection before the injection of BTX-A is condisered to be needed.

A Study of Distribution and Communities Stewartia koreana (I) - Temple Huibang and Mt. Sogri - (노각나무의 분포(分布)와 생태(生態)(I) 희방사(熹坊寺)와 속리산(俗離山)을 중심(中心)으로)

  • Kim, Chi Moon;Song, Ho Gyung
    • Korean Journal of Agricultural Science
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    • v.8 no.1
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    • pp.11-17
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    • 1981
  • The study was carried out to investigate the distribution and composition of community of Stewartia koreana in Temple Huibang and Mt. Sogri. The results obtained were as follows: 1. Within the forest floor cover species of the Temple Huibang forest, higher importance value were observed as Calamagrostis arundinacea. Sasa borealis. Lindera erythrocarpa and Lindera obtusiloba 2. In the forest floor cover species of Mt. Sogri forest, higher importance value were observed as Sasa borealis and Acer pseudo-sieboldianum var. koreanum 3. Within the floor cover species under Stewartia koreana forest, higher importance value were observed as Sasa borealis. Acer pseudo- sieboldianum var. koreanum and Calamagrostis arundinacea 4. Under shrub layer of Stewartia koreana stands, 73% of Sasa borealis and Calamagrostis arundinacea in Temple Huibang and 93% of Sasa borealis in Mt. Sogri were counted respectively as dominant species. Therefore, it might be necessary to control such dominant undergrowing species for natural reproduction of Stewartia koreana 5. In the Temple Huibang sites, by 700m Sasa borealis and 800m Calamagrostis arundinacea were grown as dominant species.

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Mitochondrial DNA Analysis of Human Skeletal Remains Excavated from Myungam-ri site in Asan, Korea (아산시 명암리 출토 인골의 미토콘드리아 DNA 분석)

  • Kim, Yun-Ji;Kim, Sue-Hoon;Cho, Eun-Min;Lee, Jeong-won
    • 보존과학연구
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    • s.36
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    • pp.33-48
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    • 2015
  • In this study, ancient DNA analyses were carried out on the human skeletal remains from a historical cemetery site in Myeongam-ri, Asan, Korea. Human remains of 27 individuals out of tombs from the Goryeo to Joseon Dynasty were selected for the analysis of this study. In order to identify the genealogy of the population and traditional burial pattern of the cemetery, we conducted comparative analyses of the hyper variable regions (HVRs) in mitochondrial DNA (mtDNA) of each sample. We sequenced 9 segmental amplicons of HVRs and assigned relevant haplogroups according to the sequence polymorphism on the basis of the known mtDNA database. As a result, we were analyses 18 human remains of 27 individuals and result of amelogenin analysis were only 4 samples.

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LIR motifs and the membrane-targeting domain are complementary in the function of RavZ

  • Park, Sang-Won;Jun, Yong-Woo;Jeon, Pureum;Lee, You-Kyung;Park, Ju-Hui;Lee, Seung-Hwan;Lee, Jin-A;Jang, Deok-Jin
    • BMB Reports
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    • v.52 no.12
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    • pp.700-705
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    • 2019
  • The bacterial effector protein RavZ is secreted by the intracellular pathogen Legionella pneumophila and inhibits host autophagy through an irreversible deconjugation of mammalian ATG8 (mATG8) proteins from autophagosome membranes. However, the roles of the LC3 interacting region (LIR) motifs in RavZ function remain unclear. In this study, we show that a membrane-targeting (MT) domain or the LIR motifs of RavZ play major or minor roles in RavZ function. A RavZ mutant that does not bind to mATG8 delipidated all forms of mATG8-phosphatidylethanolamine (PE) as efficiently as did wild-type RavZ. However, a RavZ mutant with a deletion of the MT domain selectively delipidated mATG8-PE less efficiently than did wild-type RavZ. Taken together, our results suggest that the effects of LIR motifs and the MT domain on RavZ activity are complementary and work through independent pathways.

Environmental and Ecological Characteristics of Ilex crenata var. microphylla Max. Subpopulations Habitats in the East-West Slopes in Mt. Halla National Park (한라산 동.서사면 좀꽝꽝나무 아개체군 자생지의 환경 및 생태적 특성)

  • Lee, Dong-Gon;Kim, Yong-Shik;Shin, Hyun-Tak
    • Korean Journal of Environment and Ecology
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    • v.25 no.4
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    • pp.562-572
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    • 2011
  • This study is to examine the environmental characteristics of subpopulations of Ilex crenata var. microphyllla Max. distributed in the east-west slopes of Mt. Halla National Park based on analyzing the factors of environment, vegetation and soil. The result of the TWINSAPN analysis conducted on 56 study plots between the altitudes of 500 m and 1600 m of east-west slopes of Mt. Halla National Park can be divided into five communities-Prunus maximowiczii Rupr., Manshurian fullmoon Maple, Carpinus tschonoskii Max., Daphniphyllum macropodum Miq., Quercus serrata Thunb. ex Murray and Pinus thunbergil Parl. Among these communities, the plot with high importance percentage of the subpopulation of Ilex crenata var. microphyllla Max., is the Carpinus tschonoskii Max.-Daphniphyllum macropodum Miq. included in the altitude of 600m~1200m radius. The dominant species of the upper shrub layer was Carpinus tschonoskii Max.(I.P.: 29.82%) while the dominant species of the canopy layer was Daphniphyllum macropodum Miq.(I.P.: 26.76%). For the understory layer the dominant species was Ilex crenata var. microphyllla Max.. Species diversity index was on the low end with 0.7424, indicating the stably formed climax community of Carpinus tschonoskii Max.. The analysis of soil properties showed high concentrations of organic matters, available phosphate and Ca, Mg, the high importance percentage of the of Ilex crenata var. microphyllla Max. is found in altitude of 600m~800m on the eastern slope and the altitude of 800m~1,000m radius on the west slope. This region is included in the southern part of the cold/hot forest zone, its high importance percentage of Carpinus tschonoskii Max. and low in the species diversity index of 0.7424, and has formed stable climax community of Carpinus tschonoskii Max. in Mt. Halla.

Flavonoid Profiles of Quercus mongolica Fisch. ex Ledeb. and Q. serrata Murray (Fagaceae) in Mt. Seorak, Korea: Taxonomical and Ecological Implications (설악산 신갈나무와 졸참나무의 플라보노이드 조성과 분류학적, 생태학적 의미)

  • Park, Jin Hee
    • Journal of Life Science
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    • v.24 no.10
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    • pp.1092-1101
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    • 2014
  • In this study, the distribution patterns of Quercus mongolica and Q. serrata in Korea were investigated, and the possibility of introgressive hybridization and gene flow between Q. mongolica and Q. serrata in Mt. Seorak was inferred by flavonoid analyses. The most critical factor in the vertical and horizontal distribution patterns of Q. mongolica and Q. serrata was the temperature, in accordance with latitude and altitude. The species showed a zonal distribution, with a Q. mongolica zone in the upper area and a Q. serrata zone in the lower area. In Mt. Seorak, Central Korea, the range of the vertical distribution of Q. mongolica was generally above an altitude of 100 m, whereas that of Q. serrata was an altitude of 0-400 m (-500) and rarely above an altitude of 500 m. However, in Mt. Jiri, Southern Korea, Q. serrata was found up to an altitude of 1,000~1,200 m, whereas the frequency of Q. mongolica was reduced at lower elevations and the species was rare below an altitude of 300 m, although pure stands were found on higher mountain slopes above an altitude of 1,200 m. The altitudinal distribution of the two species overlapped, where the two species occurred together. The leaf flavonoid constituents of thirty-four individuals of Q. mongolica and Q. serrata in Mt. Seorak and Mt. Jiri, Korea were examined. Twenty-four flavonoid compounds were isolated and identified. These were glycosylated derivatives of flavonols kaempferol, quercetin, isorhamnetin, myricetin. Five compounds among the flavonoid compounds were acylated. Kaempferol 3-O-glucoside, quercetin 3-O-glucoside, quercetin 3-O-galactoside, and its acylated compounds were major constituents and present in all individuals. Quercus mongolica is distinguished from Q. serrata by the presence of quercetin 3-O-arabinosylglucoside, a high concentration of three acylated compounds (kaempferol 3-O-glucoside, quercetin 3-O-glucoside, and quercetin 3-O-galactoside), and a relatively low concentration or lack of rhamnosyl flavonol compounds. Intraspecific variations, however, were found in the flavonoid profiles of Q. mongolica and Q. serrata, and the flavonoid profiles of individuals belonging to the two species in a hybrid zone (sympatric zone) tended to be similar, qualitatively and quantitatively. These findings strongly suggest that gene exchange or gene flow occurs through introgressive hybridization between Q. mongolica and Q. serrata in Mt. Seorak.

Genetic Variation and Population Specific Mitochondrial DNA Haplotype Found in the Jeju Native Pig Population (제주재래돼지 집단서 집단특이적 mtDNA Haplotype과 유전적 다양성)

  • Han, S.H.;Cho, I.C.;Lee, C.E.;Lee, S.S.;Kang, S.Y.;Choi, Y.L.;Oh, W.Y.;Sung, P.N.;Ko, S.B.;Oh, M.Y.;Ko, M.S.
    • Journal of Animal Science and Technology
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    • v.46 no.6
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    • pp.917-924
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    • 2004
  • Using PCR-RFLP haplotyping for the mitochondrial DNA(mtDNA) fragment containing the NADH dehydrogenase 2 gene(ND2) and three tRNA genes(tRNA-Met, tRNA-Trp and tRNA-Ala), we characterized the genetic diversity of five pig breeds including Jeju native pigs. mtDNA polymorphisms showing distinct cleavage patterns were found in the pig breeds. Two digestion patterns were detected when HaeIII- and Hinfl-RFLP, and four in the Tsp5091-RFLP analyses. Combining the three restriction enzyme digestion patterns found in five different pig breeds, four mtDNA haplotypes were observed and the haplotype frequencies were significantly different by the pig breeds. A monomorphic haplotype, mtWB, was observed in both Korean wild boars and Large White pigs. Both Duroc and Landrace pigs contained two haplotypes suggesting their multiple maternal lineages. Jeju native pig has two haplotypes(mtJN and mtJD). Of these, mtJN is identified as a Jeju native pig specific haplotype. This study suggested that more than two progenitor populations have been taken part in the domestication process of the Jeju native pig population, and/or probably subsequent crossing with other pig breeds from near east Asia. Unlike with our prediction, there was no direct evidence under molecular levels on the maternal introgression of Korean wild boar in the domestication of Jeju native pigs. In conclusion, specificity of mtDNA haplotypes related to pig breeds win be useful for identifying the maternal lineage as wen as constructing the genealogical pedigree in pigs.

Vegetation Structure and Site Environment of Natural Habitat of an Endangered Plant, Viola websteri (멸종위기 식물 왕제비꽃 자생지의 식생구조 및 입지환경)

  • Song, Jae-Mo;Lee, Gi-Yeoung;Kim, Nam-Young;Yi, Jae-Seon
    • Journal of Korean Society of Forest Science
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    • v.99 no.3
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    • pp.267-276
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    • 2010
  • This study was performed to obtain basic data about vegetation structure and site environment of rare and endangered plant, viola websteri in five natural habitats. The dominant species of Hongcheon habitat were Pyrus pyrifolia (tree layer), Morus bombycis (subtree layer), Pyrus pyrifolia (shrub layer) and Isodon inflexus (herb layer). Mt. Yeonin habitat were dominated by Quercus mongolica (tree layer), Morus bombycis (subtree layer), Philadelphus schrenckii (shrub layer) and Viola websteri (herb layer). Mt. Yumyung habitat were dominated by Cornus controversa (tree layer), Morus bombycis (subtree layer), Fraxinus rhynchophylla (shrub layer) and Adenocaulon himalaicum (herb layer). Mt. Daebu habitat were dominated by Acer mono (tree layer), Staphylea bumalda (subtree layer), Staphylea bumalda(shrub layer) and Dryopteris crassirhizoma (herb layer). And the dominant species of Mt. Worak habitat were Larix leptolepis (tree layer), Fraxinus rhynchophylla (subtree layer), Alangium platanifolium var. macrophylum (shrub layer) and Adenocaulon himalaicum (herb layer). It was found out that the average acidity of soil pH was 5.23; 0.30ds/m for electron conductivity; 12.6% for organic matter content; 0.68% for total nitrogen; 21.66 ppm for available phosphorous; and 23.45 cmol(+)/kg for CEC. Interchangeable cation was decreased in order of $Ca^{2+}$ > $Mg^{2+}$ > $K^{2+}$. Viola websteri had positive association with Polygonatum odoratum var. pluriflorum and Geranium thunbergii but negative association with Poa sphondylodes. Species diversity index(H'), maximum Species diversity index (H' max), evenness(J') and dominance (1-J'), of investigated sites ranged 0.6816 (Mt. Worak)~0.9656 (Mt. Daebu), 1.0000 (Mt. Yeonin)~1.3010 (Hongcheon, Gangwon), 0.5330 (Mt. Worak)~0.8947 (Mt. Daebu) and 0.1053 (Mt. Daebu)~0.4670 (Mt. Worak) in woody layer and 1.0414 (Mt. Yumyung)~1.3295 (Mt. Worak), 1.4314 (Mt. Yumyung)~1.6435 (Mt. Daebu), 0.6984 (Hongcheon, Gangwon)~0.8356 (Mt. Worak) and 0.1644 (Mt. Worak)~0.3016 (Hongcheon, Gangwon) in herb layer, respectively.

Structure and Dynamics of Lindera sericea Community in Mt. Chogye, Korea (조계산 털조장나무군낙의 구조와 동태)

  • Kim, J. H.
    • The Korean Journal of Ecology
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    • v.20 no.1
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    • pp.15-25
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    • 1997
  • The deciduous shrubs, Lindera sericea belonging to family Lauracea are distributed in Mt. Chogye and Mudeung locally. To estimate the community structure of this shrub, the relationship between the floristic composition and environmental factors of 20 sites in Mt. Chogye were investigated. L. sericea community distributed at the hum양 zone of altitude 305-480 m in ES and NE slope of $15~30^{\circ}$. The floristic composition of this communities consisted of 17-32 species varied with sites. Sasa borealis, Sapium japonicum, and Quercus serrata were the constant species over 90%. The forest vegetation was classified as Sasa borealis-Lindera sericea community, and the Sasa borealis-Meliosma oldhamii, and Sasa borealis-Callicarpa japonica were as subunit community. Acidity, organic matter content, inorganic phophate, potassium, calcium, magnesium, cation exchange capacity and conductivity were determined as environmental factors. Diameter of stem at ground surface and stem, distribution area and growth property of this plant were investigated, and this plant was presumed to be a relic endemic species.

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