• Preventive Nutrition and Food Science
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• v.21 no.4
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• pp.317-322
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• 2016

Mitochondrial biogenesis is a complex process requiring coordinated expression of nuclear and mitochondrial genomes. The peroxisome proliferator-activated receptor gamma co-activator 1-alpha (PGC-$1{\alpha}$) is a key regulator of mitochondrial biogenesis, and it controls mitochondrial DNA (mtDNA) replication within diverse tissues, including muscle tissue. The aim of this study was to investigate the effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on mtDNA copy number and PGC-$1{\alpha}$ promoter activity in $C_2C_{12}$ muscle cells. mtDNA copy number and mRNA levels of genes related to mitochondrial biogenesis such as PGC-$1{\alpha}$, nuclear respiratory factor 1 (NRF1) and mitochondrial transcription factor A (Tfam) were assayed by quantitative real-time PCR. The PGC-$1{\alpha}$ promoter from -970 to +412 bp was subcloned into the pGL3-basic vector, which includes a luciferase reporter gene. Both EPA and DHA significantly increased mtDNA copy number, dose and time dependently, and up-regulated mRNA levels of PGC-$1{\alpha}$, NRF1, and Tfam. Furthermore, EPA and DHA stimulated PGC-$1{\alpha}$ promoter activity in a dose-dependent manner. These results suggest that EPA and DHA may modulate mitochondrial biogenesis, which was partially associated with increased mtDNA replication and PGC-$1{\alpha}$ gene expression in $C_2C_{12}$ muscle cells.

• Journal of Embryo Transfer
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• v.18 no.3
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• pp.227-235
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• 2003

한우의 mt DNA cytochrome oxidase subunit I, II, 및 III complex지역의 유전적 다형현상을 제한효소를 이용하여 검출하였다. PCR primer 6종에 대하여 20가지 제한효소를 처리하였으며, Pst I, Pvu II, Rsa I, Eco RI, Bgl II, and Msp I 제한효소를 사용하여 유전적 변이를 검출하였다. 검출된 변이체와 한우의 성장과의 관련성을 조사한 결과 cytochrome oxidase subunit III complex 지역의 유전염기서열을 근거로 제작한 primer Mt9 좌위에서 제한효소 PvuII를 이용한 절단형과 체중형질 인 WT15(P<0.05) 및 WT18(P<0.01)에서 고도의 유의성이 관찰되었다. 아울러 , Mt9-Pvu II(P=0.07), Mt6-Bgl II(P=0.05), and Mt8-Rsa I(P=0.05) 좌위 또한 WT9, WTl5, and WT15에서 각각 통계적 유의성이 관찰되었다. 따라서 본 결과는 cytochrome oxidase subunit III complex segments가 candidate gene으로서 기초적 유전정 보 제공은 물론 유전적 개량을 위해 사용될 수 있을 것으로 사료된다.

• Journal of Nutrition and Health
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• v.52 no.2
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• pp.139-148
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• 2019

Purpose: Ulcerative colitis is a common inflammatory bowel disease. Prolonged colitis can be a risk factor for the development of colorectal cancer. Mulberry twig (MT, Sangzhi), a dry branch of Morus alba L., which is widely distributed throughout East Asia, has been shown to have anti-inflammatory activities in the cells. However, the effects of MT extracts on colitis in in vivo are limited. Therefore, in this study, we investigated the anti-inflammatory effects of MT extracts in the dextran sulfate sodium (DSS)-induced mouse colitis model. Methods: Six week-old, male ICR mice were divided into 3 groups: Control (n = 5), DSS (n = 7), and DSS+MT (n = 7) groups. Mice in the DSS and DSS+MT groups were administrated 3% DSS in drinking water for 5 days to induce colitis. At the same time, water extracts of MT (5 g/kg body weight/day) were orally administered to mice in the DSS+MT groups for 5 days. Results: The MT extracts significantly reduced the clinical and pathological characteristics of colitis. Disease activity index, mucosal thickness, and colonocyte proliferation were significantly reduced in the DSS+MT group compared with the DSS group. Furthermore, MT administration reduced the levels of plasma $TNF-{\alpha}$, IL-6, and the colonic myeloperoxidase activity as well as mRNA expression of $TNF-{\alpha}$, IL-6, Cox-2, and iNOS. Conclusion: Taken together, these results suggest that MT water extracts have potent anti-colitis activities in the mouse colitis model.

• Korean Journal of Plant Tissue Culture
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• v.27 no.6
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• pp.453-459
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• 2000

Three rice (Oryza sativa L. var Dong-Jin) mutants (DTR1, DTR2, DTR3) resistant to S-methyltryptophan (5MT) were selected by mutagenized M3 seeds. The frequency of chlorophyll mutations induced by the EMS (0.2%) treatment performed 2 hours after flowering is clearly higher than that induced by other treatments in M1 generation. Progeny obtained from the self-pollinating of 5MT-resistant lines segregated with 3 : 1 of resistant to sensitive ratio. Furthermore, the ratio of homozygote to heterozygote in 5MT-resistant plants of the M4 generation was 1：2. These results show that 5MT resistance was inherited as a single dominant nuclear gene. The resistance was also expressed in callus derived from seeds. Total free amino acid content in homozygous seeds of DTR1 and DTR2 showed about 1.7 fold-increased compared to the wild-type seeds. In particular, the levels of phenylalanine and Iysine were, respectively, 6.2 and 3.2 times higher than those in the wild-type seeds. However, seeds of DTR3 had lower levels of free amino acid than the wild-type seeds. This result indicate that these mutants as a significant step towards the production of new rice with balanced amino acid content.

• The Korean Journal of Ecology
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• v.16 no.3
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• pp.287-303
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• 1993

A phytosociological study of forest vegetation of Mt. Kaya was carried out from August 1988 to September 1992. The forest vegetation of the area was classified into 3 communities (Quercus monogolica community, Lindera erythrocarpa community, Pinus densiflora community) and 3 subunits (Rhododendron mucronulatum subunit, Platycarya strobilacea subunit, typical subunit of Pinusdensiflora community). The Pinus densiflora community was located at the lower altitudes (100~300m) than those of other communities. The Lindera erythrocarpa community was located at the upper altitude (500m). Most of the inverstigated area were occupied by the secondary forest of Pinus densiflora community in the actual vegetation map and the profile diagram, suggesting that it was frequently destroyed by human interference

• Plant Resources
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• v.2 no.2
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• pp.113-126
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• 1999

Through the research of tracheophytes type around Mt. Daedun, we are aiming at figuring out the habitat condition of useful resource plants by the classification of vegetation units and communities using the Z-M phyto-sociological method in the basis of the traits of species composition and by the analysis of vegetation and environment by way of coincidence method. Tracheophytes in our research sites was classifid into 117 family, 475 genus 797, species 102 variety,18 breed, in total to 917 kinds. The forest around Mt. Daedun district was classified into 6 communities, 12 groups, 2 sub-groups according to feature species group, differential species group and differential. Judging the result from the examination of community classification factors by the coincidence method, vegetation unit was decided by altitude and topography.

• The Korean Journal of Ecology
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• v.18 no.1
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• pp.179-188
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• 1995

Death patterns of gap-makers and changes of environmental factors in gaps formed by disturbance were studied in Pinus densiflora forests of Youngwol, Mt, Mansu, Mt. Songni, Uljin, and Mt, Obong in Korea. Death pattern of gap-makers showed that standing deat type was the most frequent in all the study areas. Longevity of Pinus densiflora was about 140 years, which was about half of that of the temperate deciduous broad-leaved trees. size of gaps were distributed from $20m^2$ to $235m^2$, more than 80% of those were gaps created by death of two or more trees. Relative light intensity and water content of soil in gap area were higher than those in non-gap area and those in the central part of gap were the highest.

• Korean Journal of Soil Science and Fertilizer
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• v.16 no.3
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• pp.280-285
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• 1983

The effect of pH and calcium on the growth of subterraneum clover (Trifolium subteraneum L.) varieties Dwalganup, Mt. Barker, and Tallarook was determined in solution culture. At pH 4.5 and below all components of growth except germination percentage were adversely affected. The effect of lower pH was improved at high calcium level(3.0mM). There was a varietal difference in response to low pH. Mt. Barker showed the highest percent germination and produced the longest radicle length, whereas the greatest value in top dry weight, root dry weight, root length and number of laterals was obtained with Tallarook.

• The Korean Journal of Ecology
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• v.17 no.2
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• pp.185-201
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• 1994

The Quercus mongolica forest vegetation of Mt. Myongji was classified into two communities including four subunit communities and one typical subunit community by the Z-M method as follows: Acer pseudo-sieboldianum-Quercus mongolica community group Quercus mongolica - Isodon excisus community Quercus mongolica - Styrax obassia community Quercus mongolica - Lespedeza bicolor subunit coummunity Quercus mongolica - Aconitum longecassidatum subunit community Quercus mongolica - Rhododendrom schlippenbachii subunit community Quercus mongolica - Cornus controversa subunit community Quercus mongolica - Styrax obassia typical subunit community Acer pseudo-sieboldianum - Quercus mongolica community group was distributed over the upper region of the altitude 400m, and the differential species in the community were Carex siderosticata, Sephanandra incisa, Tripterygium regelii, and Fraxinus rhynchophylla. The vegetation patterns for the slope and azimuth showed that the highest importance value for Quercus mongolica forest was observed on the broad subxeric area, and for Carpinus cordata and Acer pseudo-sieboldianum on the wet site, for Acer mono, Styrax obassia, Fraxinus rhynchophylla and Symplocos chinensis for. pilosa on the xeric site. The best habitat condition for Quercus mongolica was found at a subxeric site at the altitude of 700-900m on southern slope and that for Acer pseudo-sieboldianum at the 700-1100m on northern slope.

• The Korean Journal of Ecology
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• v.17 no.3
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• pp.415-424
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• 1994

Community structure, size and age distribution of tree species of the subalpine Korean fir (Abies koreana) forest at the elevation of 1, 400~1, 700m were strudied in the north-west side of Chonwangbong Peak (1915m) in Mt. Chiri for the purpose of better understanding of the population dynamics of Abies koreana. Eight 20m X 20m permanent quadrats were established in 1991, and trees $\geq$ 2.5cm DBH for all species were marked with numbered aluminum tag, and saplings of Korean fir and Korean pine (Pinus koraiensis) were also tagged with aluminum sheet. These two conifer species comprised more than 60% of the total density and total basal area of the community. Quercus mongolica and Acer pseudo-sieboldianum were subdominats. Oridination study showed that cool temperate species such as Sorbus commixta, Betula costata, Acer tschonoskii and Acer ukurunduense occurred in close association with Abies koreana and Pinus koraiensis. Major tree species, especially Abies koreana and Pinus koraiensis, were well represented in samller size classes, indicating that they were regenerating well. Age distributuion of the Abies koreana and Pinus koraiensis showed that the former has longer physiological longevity than the latter, and that establishment were episodic and varied with sites, which implies the importance of the role of natural and artificial disturbances in this Abies kireana forest.