• Journal of Microbiology and Biotechnology
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• v.20 no.6
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• pp.1022-1026
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• 2010

The different cleavage patterns of pYBamy59 plasmid isolated from E. coli $DH5{\alpha}$ and B. longum MG1 by the cell extract of B. longum MG1 suggested that the main reason for its low transformation efficiency was related to the restriction modification (R-M) system. To confirm the correlation between the R-M system and transformation efficiency, in vitro methylation and site-directed mutagenesis were performed in pYBamy59. Sequence analysis of pYBamy59 fragments digested by the cell extract of B. longum MG1 revealed that all fragments were generated by restriction of the sequence recognized by SacII endonuclease. When pYBamy59 from E. coli was methylated in vitro by CpG or GpC methyltransferase, it was protected from SacII digestion. Site-directed mutagenesis, which removed SacII sites from pYBamy59, or in vitro methylation of pYBamy59 showed 8- to 15-fold increases in the transformation efficiency over intact pYBamy59. Modification of the SacII-related R-M system in B. longum MG1 and in vitro methylation in pYBamy 59 can improve the transformation efficiency in this strain. The results showed that the R-M system is a factor to limit introduction of exogenous DNA, and in vitro modification is a convenient method to overcome the barrier of the R-M system for transformation.

• Journal of Advanced Navigation Technology
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• v.21 no.1
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• pp.132-137
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• 2017

This paper proposes a transformation method of the zero moment point (ZMP) and the center of mass (CoM) from one walking pattern to other patterns by considering the structure of a robot or walking situations in real time. In general, a humanoid robot has own structure characteristics like height and mass. The structure characteristics make the given CoM/ZMP walking pattern of one human or one humanoid robot to be difficult to apply to other robot directly. For this purpose, we analyze the characteristics of walking patterns according to the step length, duration of walking support phase and the CoM height by using the cart-table model as the simple humanoid robot model. A transformation equation is derived from the analyzation and it is verified with simulation.

• Journal of Korea Society of Digital Industry and Information Management
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• v.9 no.1
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• pp.165-176
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• 2013

This paper introduces the high density discrete wavelet transform using quincunx sampling, which is a discrete wavelet transformation that combines the high density discrete transformation and non-separable processing method, each of which has its own characteristics and advantages. The high density discrete wavelet transformation is one that expands an N point signal to M transform coefficients with M > N. The high density discrete wavelet transformation is a new set of dyadic wavelet transformation with two generators. The construction provides a higher sampling in both time and frequency. This new transform is approximately shift-invariant and has intermediate scales. In two dimensions, this transform outperforms the standard discrete wavelet transformation in terms of shift-invariant. Although the transformation utilizes more wavelets, sampling rates are high costs and some lack a dominant spatial orientation, which prevents them from being able to isolate those directions. A solution to this problem is a non separable method. The quincunx lattice is a non-separable sampling method in image processing. It treats the different directions more homogeneously than the separable two dimensional schemes. Proposed wavelet transformation can generate sub-images of multiple degrees rotated versions. Therefore, This method services good performance in image processing fields.

• Journal of Korea Society of Digital Industry and Information Management
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• v.8 no.3
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• pp.133-145
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• 2012

This paper describes the high density discrete wavelet transformation which is one that expands an N point signal to M transform coefficients with M > N. The double-density discrete wavelet transform is one of the high density discrete wavelet transformation. This transformation employs one scaling function and two distinct wavelets, which are designed to be offset from one another by one half. And it is nearly shift-invariant. Similarly, triple-density discrete wavelet transformation is a new set of dyadic wavelet transformation with two generators. The construction provides a higher sampling in both time and frequency. Specifically, the spectrum of the first wavelet is concentrated halfway between the spectrum of the second wavelet and the spectrum of its dilated version. In addition, the second wavelet is translated by half-integers rather than whole-integers in the frame construction. This arrangement leads to high density wavelet transformation. But this new transform is approximately shift-invariant and has intermediate scales. In two dimensions, this transform outperforms the standard and double-density discrete wavelet transformation in terms of multiple directions. Resultingly, the proposed wavelet transformation services good performance in image and video processing fields.

• Environmental Mutagens and Carcinogens
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• v.21 no.1
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• pp.44-50
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• 2001

To identify nongenotoxic carcinogen determined as negative by ICH guideline-recommended standard genotoxicity test battery; Ames test, chromosome aberration assay, mouse lymphoma $tk^{+/-}$ assay, in vivo micronucleus assay, we picked bisphenol A as a model compound. In this study, we applied in vitro BalB/c 3T3 cell transformation assay and Syrian hamster embryo (SHE) cell transfarmation assay. Bisphenol A was treated upto $769.2 ug/m{\ell}$ in BalB/c 3T3 cells and upto $125 ug/m{\ell}$ in SHE cells. bisphenol A didn't induced morphological transformation both with one stage treatment protocol and with two stage treatment protocol. But, treated far 48 hr, Bisphenol A induced morphological transformation significantly in SHE cells.

• Korean Journal of Veterinary Research
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• v.24 no.1
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• pp.40-49
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• 1984

To investigate the factors influencing the artifical transformation in Escherichia coli, E. coli C600 was transformed by pBR322 DNA with tetracycline and ampicillin resistant gene purified by CsCl-Etbr equilibrium density gradient centrifugation from E.coli HB 101. The influencing factors in the transformation such as concentration of calcium chloride, time of ice incubation, temperature and time of heat shock, time of gene expression, effects of plasmid DNA concentration and adding time were examined in these experiments. The results obtained were as follows; 1. The highest transformation frequency was observed in the treatments of 100 mM $CaCl_2$ before heat shock and the treatment of $CaCl_2$ was essential step in the process of E. coli transformation. 2. The highest transformation frequency was observed in the treatment of heat shock at $42^{\circ}C$ for 4 min. or $37^{\circ}C$ for 6 min., but the prolonged heat shock resulted a decreased transformation frequency. 3. Treatments of ice incubation at $0^{\circ}C$ for 45 min. before heat stocks or at $0^{\circ}C$ for 30min. after heat shock resulted an increased transformation frequency. 4. There was a linear relationship between DNA concentration and transformation frequency at the concentration of $8{\times}10^3$ recipient cells. The highest transformation frequency reached in carte of 7 mcg of donor DNA, but above 1 mcg of DNA concentration, transformation frequency was not remarkably increased. Addition of donor DNA just after the treatment of $CaCl_2$ was the best. 5. The best condition of gene expression at $37^{\circ}C$ were 40min. for TC-resistant gene and 100min. for AP-resistant gene. TC-resistant gene was higher in the transformation frequency and faster in the gene expression time than AP-resistant gene. In these results, the best conditions for the transformation of E. coli C 600 with pBR322 DNA were: treatment with 100mM $CaCl_2$, ice incubation at $0^{\circ}C$ for 45 min, heat shock at $42^{\circ}C$ for 4 min., 30 min. of ice incubation and incubation at $37^{\circ}C$ for 100min. for gene expression in that order.

• Journal of Welding and Joining
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• v.35 no.1
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• pp.89-94
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• 2017

The microstructure and dilatation for 0.15C steels were investigated to define the phase transformation during the quenching and partitioning (Q&P) process. For the one step Q&P dilatation, the isothermal martensite/bainite transformation occurred because the holding temperature was between $M_s$ and $M_f$. The isothermally transformed martensite/bainite and the athermally transformed martensite were produced by a loss of retained austenite. As the holding time increased, new martensite-start ($M_s$) temperature produced from the final quenching process decreased due to the carbon partitioning from the martensite to the retained austenite. This was the direct evidence of increment for the retained austenite stability. For the two step Q&P dilatation, the isothermal bainitic transformation occurred because the partitioning temperature was larger than the $M_s$ and new $M_s$. The partitioning at $400^{\circ}C$ indicated the short incubation period for the bainite transformation than the $350^{\circ}C$ partitioning because the partitioning at $400^{\circ}C$ should acquire the larger thermal driving force for carbon partitioning than the $350^{\circ}C$ partitioning. A quick drop of $M_s$ and short period of bainite incubation for the $400^{\circ}C$ partitioning steel were also the direct evidence of significant effects of carbon partitioning on the stability of retained austenite.

• The Journal of Advanced Prosthodontics
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• v.11 no.5
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• pp.253-261
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• 2019

PURPOSE. To evaluate the influence of cyclic loading on phase transformation of zirconia abutments and to compare the effectiveness of three different quantitative ageing assessment techniques. MATERIALS AND METHODS. Thirty two Y-TZP prostheses fabricated from two brands, InCoris ZI and Ceramill ZI, were cemented to titanium bases and equally divided into two subgroups (n=8): control group without any treatment and aged group with cyclic loading between 20 N and 98 N for 100,000 cycles at 4 Hz in distilled water at $37^{\circ}C$. The tetragonal-to-monoclinic phase transformation was assessed by (i) conventional x-ray diffraction (XRD), (ii) micro x-ray diffraction (${\mu}XRD$), and (iii) micro-Raman spectroscopy. The monoclinic-phase fractions (M%) were compared by two-way ANOVA. RESULTS. InCoris Zi presented significantly higher M% than Ceramill Zi in both control and aged groups (P<.001). Both materials exhibited significant phase transformation with monoclinicphase of 1 to 3% more in aged groups than controls for all three assessment techniques. The comparable M% was quantified by both ${\mu}XRD$ and XRD. The highest M% was assessed with micro-Raman. CONCLUSION. Cyclic loading produced significant phase transformation in tested Y-TZP prostheses. The micro-Raman spectroscopy could be used as an alternative to XRD and ${\mu}XRD$.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.13 no.4
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• pp.179-191
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• 2013

The quincunx lattice is a non-separable sampling method in image processing. It treats the different directions more homogeneously and good frequency property than the separable two dimensional schemes. The high density discrete wavelet transformation is one that expands an N point signal to M transform coefficients with M > N. In two dimensions, this transform outperforms the standard discrete wavelet transformation in terms of shift-invariant. Although the transformation utilizes more wavelets, sampling rates are high costs. This paper proposed the high density discrete wavelet transform using quincunx sampling, which is a discrete wavelet transformation that combines the high density discrete transformation and non-separable processing method, each of which has its own characteristics and advantages. Proposed wavelet transformation can service good performance in image processing fields.

• Communications for Statistical Applications and Methods
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• v.7 no.2
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• pp.574-574
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• 2000

Consider the problem of estimating regression function from a set of data which is contaminated by a long-tailed error distribution. The linear smoother is a kind of a local weighted average of response, so it is not robust against outliers. The kernel M-smoother and the lowess attain robustness against outliers by down-weighting outliers. However, the kernel M-smoother and the lowess requires the iteration for computing the robustness weights, and as Wang and Scott(1994) pointed out, the requirement of iteration is not a desirable property. In this article, we propose the robust nonparametic regression method which does not require the iteration. Robustness can be achieved not only by down-weighting outliers but also by transforming outliers. The rank transformation is a simple procedure where the data are replaced by their corresponding ranks. Iman and Conover(1979) showed the fact that the rank transformation is a robust and powerful procedure in the linear regression. In this paper, we show that we can also use the rank transformation to nonparametric regression to achieve the robustness.