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Effect of Physicochemical Treatment on Growth Inhibition of Hanseniaspora uvarum Y1 from Yogurt (물리·화학적 처리에 의한 요구르트 오염균의 생육 억제효과)

  • SunWoo, Chan;Lee, So-Young;Yoon, So-Young;Jung, Ji-Yeon;Kim, Koth-Bong-Woo-Ri;Lee, Chung-Jo;Kwak, Ji-Hee;Kim, Min-Ji;Kim, Dong-Hyun;Jung, Seul-A;Kim, Hyun-Jee;Ahn, Dong-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.12
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    • pp.1781-1786
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    • 2011
  • This study was conducted to investigate the cause of microbiological contamination in yogurt and evaluate the effect of physicochemical treatment on the growth inhibition of Hanseniaspora uvarum isolated from yogurt. The yeast strain Hanseniaspora uvarum Y1 was subjected to heat and pH treatments. H. uvarum Y1 was killed at $70^{\circ}C$ and $80^{\circ}C$ after 15 min and survived in a wide pH range from pH 2 to 9. However, it did not survive under pH 1 and over pH 10. In a disk diffusion susceptibility test on H. uvarum Y1, a clear zone (5 mm) of growth inhibition was observed upon treatment with electrolyzed water. The effect of ozone gas on the growth of H. uvarum Y1 was evaluated by viable cell count. Initial cell numbers of $10^2$ and $10^3$ CFU/mL of H. uvarum Y1 were completely killed by treatment for 10 and 30 min, respectively. H. uvarum Y1 was also sterilized by microwave treatment for 1 min. When treated with gamma-irradiation, the rate of killing of H. uvarum Y1 was proportional to the irradiation dose. and complete killing occurred at a dose of 50 kGy.

Effects of Thermal and Mechanical Fatigue Stress on Bond Strength in Bracket Base Configurations (열적, 기계적 피로응력이 교정용 브라켓의 결합강도에 미치는 영향)

  • Kim, Jong-Ghee;Kim, Sang-Cheol
    • The korean journal of orthodontics
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    • v.30 no.5 s.82
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    • pp.625-642
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    • 2000
  • The purpose of this study is to evaluate the effects of mechanical and thermal fatigue stress on the shear, tensile and shear-tensile combined bond strengths(SBS, TBS, CBS) in various orthodontic brackets bonded to human premolars with chemically cured adhesive(Ortho-one, Bisco, USA). Five types of commercially available metal brackets with various bracket base configurations of Photoetched base(Tomy, Japan), Non-Etched Foil Mesh base(Dentaurum, Germany), Micro-Etched Foil Mesh base(Ortho Organizers, USA), Chessboard base(Daesung, Korea), and Integral base(3M Unitek, USA) were used. Samples were divided into 3 groups, the first group was acted with shear-tensile combined loads($45^{\circ}$) of 200g for 4 weeks(mechanical fatigue stress), the second group was subjected to the 5,000 thermocycles of 15 second dwell time each in $5^{\circ}C\;and\;55^{\circ}C$ baths(thermal fatigue stress), and the third group was the control. Bond strengths were measured at the crosshead speed of 0.5mm/min. The cross-section of bracket base/adhesive interface and the fracture surface were examined with the stereoscope and the scanning electron microscope. The resin remnant on bracket base surface was assessed by ART(Adhesive Remnant Index). The obtained results were summarized as follows, 1. In static bond strength, Photoetched base bracket showed the maximum bond strength and Integral base bracket showed the minimum bond strength(p<0.05). In all brackets, shear bond strength(SBS) was in the greatest value and shear-tensile combined strength(CBS) was in the least value(p<0.05). 2. After mechanical fatigue test, Photoetched base bracket showed the maximum bond strength and Integral base bracket showed the minimum bond strength(p<0.05). In Photoetched base bracket and Micro-Etched Foil Mesh base bracket, shear bond strength(SBS), tensile bond strength(TBS) and shear-tensile combined strength(CBS) were decreased after mechanical fatigue test(p

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Dendrochronological Analysis of Abies koreana W. at Mt. Halla, Korea: Effects of Climate Change on the Growths (한라산 구상나무(Abies koreana W.)의 연륜연대학적 연구 - 기후변화에 따른 생장변동 분석 -)

  • Koo, Kyung-Ah;Park, Won-Kyu;Kong, Woo-Seok
    • The Korean Journal of Ecology
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    • v.24 no.5
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    • pp.281-288
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    • 2001
  • The relationships between the growths of Abies koreana W. and climatic factors were analyzed by the use of tree-ring analysis at the subalpine belt of Mt. Halla National Park. The four cores were extracted from each 21 trees at north-facing slope (1,900m a.s.1.). The site chronology was established on the periods from 1912 to 1999. The growth of A. koreana was very poor, in particular in the years of 1982, 1988 and 1996. Simple correlation was employed to analyze the relationship between the growth of A. koreana and climatic factors. The result of simple correlation indicates that the growth of A. koreana represent positive correlations both with the mean temperatures of April and previous November, and the precipitation of previous December and January. The presence of large number of frost-damaged scars in the individual trees of A. koreana implies that local freezing temperature conditions at Mt. Halla have occurred in 1964, 1965 and 1966. The correlations between the fir chronology SOI(Southern Oscillation Index) of previous January, February and November were significantly positive. The growth ratio of A. koreana demonstrates that this species is sensitive to seasonal variations. As the winter temperature rises, the growth ratio of A. koreana decreases, on the other hand, the increase of autumn temperature accelerates the growth ratio of A. koreana. The growth decline of A. koreana was observed from 51 cores out of the 54 cores, and the overall growth declines have initiated at 1978, 1982 and 1988. Distinct growth decline of A. koreana in the range of 70% is noticed at 34 cores out of the 51 cores. The decline of, A. koreana growth appears to be related to the winter temperature which has increased since mid-1970s.

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What Factors Affect Mortality over the Age of 40? (40세 이후의 사망에 영향을 주는 요인에 관한 코호트내 환자-대조군 연구)

  • Park, Jong-Ku;Koh, Sang-Baek;Kim, Chun-Bae;Park, Kee-Ho;Wang, Seung-Jun;Chang, Sei-Jin;Sin, Soon-Ae;Kang, Myung-Guen
    • Journal of Preventive Medicine and Public Health
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    • v.32 no.3
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    • pp.383-394
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    • 1999
  • Objectives: This study was conducted to identify the factors influencing the mortality of Koreans over the age of 40 by a nested case-control study. Methods: The cohort consisted of the beneficiaries of Korea Medical Insurance Corporation for Government Employees & Private School Teachers and Staff(KMIC) who received health examinations of KMIC in 1992 and 1993 retrospectively. At that time, they were more than 40 years old. The cases were 19,258 cohort members who had died until December 31, 1997. The controls were 19,258 cohort members who were alive until December 31, 1997. Controls were matched with age and sex distribution of the cases. The data used in this study were the funeral expenses requesting files, and the files of health examinations and health questionnaires gathered in 1992 and 1993. To assess the putative risk factors of death, student t-test, chi-square test, multiple logistic regression analysis were used. Results : In multiple logistic regression analysis, independent risk factors of death were as follows; systolic blood pressure, diastolic blood pressure, blood glucose, AST, urine glucose, urine protein, alcohol drinking(frequency), cigarette smoking and perceived health status, intake of restoratives and blood transfusion showed positive associations with death; coffee consumption showed negative associations with death; and body mass index and serum total cholesterol showed J-shaped association with death. Conclusions: Regarding the direction of association, the result of analysis on the data restricted to '96-'97 was same as that of '93-'97. But in some variables such as obesity, serum cholesterol, the odds ratios of death in the data of '96-'97 were higer than those of '93-'94, which suggested that the data of '93-'94 was bearing effect-cause relationship. We concluded that it suggested further researches using long-term follow-up data to be needed in this area.

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Association between Genetic Polymorphism of Peroxisome Proliferator-Activated Receptor Alpha Leu162Val and Metabolioc Syndrome in Korean (한국인에서 peroxisome proliferator-activated receptor alpha Leu162Val 유전자 다형성과 대사증후군간의 관련성)

  • Shin Soung-Cheal;Song Hye-Soon;Hong Young-Seoub;Kwak Jong-Young;Yoo Byung-Chul;Lee Yong-Hwan
    • Journal of Life Science
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    • v.16 no.2 s.75
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    • pp.199-205
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    • 2006
  • Peroxisome proliferator activated receptor (PPAR)-$\alpha$ of three PPAR subtypes ($-\alpha,\;-\beta/-\gamma,\;-\delta$), which are members of the nuclear hormone receptor superfamily of ligand-activated transcription factors, plays a key role in lipoprotein and glucose homeostasis. A variation in the PPAR-a gene expression has been suggested to influence the development of metabolic syndrome through alterations in lipid concentrations. The aim of our study was to investigate the association between the PPAR-a and metabolic syndrome among South Korean. A total of 542 health screen examinees were enrolled in this study who were examined in Kosin University Gospel Hospital from December, 2004 to July, 2005. The height, weight, waist circumference, and systolic and diastolic blood pressure of the subjects were examined and fasting blood glucose, total cholesterol, HDL cholesterol, LDL cholesterol, triglyceride were measured by-sampling in venous blood. The metabolic syndrome was defined as the presence of three or more of the following : waist circumference men ${\geq}90cm$, women ${\geq}80cm$, blood pressure ${\geq}130/85mmHg$, fasting glucose ${\geq}110mg/dL$, HDL cholesterol men <40 mg/dL, women <50 mg/dL, triglyceride ${\geq}150mg/dL$. The blood pressure, fasting glucose, HDL cholesterol, triglyceride were evaluated by using the criteria of NECP ATP III and waist circumference was assessed by using the criteria of WHO Asia-Western Pacific. And the author compared the frequency of the PPAR-$\alpha$ mutation of L162V ($C{\rightarrow}G$ variant in exon 5) in a sample of 542 subjects with and without the metabolic syndrome by polymerase chain reaction allele-specific oligonucleotide (PCR-ASO) method. One (0.2%) hetero-isotype among high risk of metabolic syndrome was identified. The values of waist circumference, body mass index and low density lipoprotein cholesterol of the mutant were 100 cm, 28.6 $kg/m^2$ and 120 mg/dL, respectively. Although the author failed to see significant association between the presence of the PPAR-$\alpha$ L162V polymorphism and metabolic syndrome, one PPAR-$\alpha$ L162V polymorphism in metabolic syndrome patients was found.

Night Eating and Nutrient Intake Status according to Residence Type in University Students (일부 대학생의 거주형태에 따른 야식 및 영양소 섭취 상태)

  • Jun, Ye-Sook;Choi, Mi-Kyeong;Bae, Yun-Jung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.2
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    • pp.216-225
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    • 2015
  • The purpose of this study was to evaluate night time eating habits, dietary habits, and nutrient intake in university students according to residence type. A survey was conducted by administering questionnaires to 664 students. Questionnaire interview and 24-h dietary recall were conducted. Subjects were divided into three groups according to residence type: dormitory boarding (DB group, N=313), self-boarding (SB group, N=246), and living with parents (LWP group, N=105). Average ages in the DB, SB, and LWP groups were 21.3, 22.2, and 22.1 years, respectively. There were no significant differences in body mass index between the three groups. In total, 77.3% of students regularly ate night time snacks. The proportion of students who reported night time eating was 84.0% in the DB group, 73.6% in the SB group, and 65.7% in the LWP group (P<0.001). In terms of food types consumed during night time eating, the DB group showed a significantly higher rate of consumption of fried chicken and flour-based foods than the SB and LWP groups, whereas the SB group showed a significantly higher rate of consumption of alcohol beverages than the DB and LWP groups. Energy, carbohydrates, protein, fat, vitamins, and mineral intakes were significantly higher in the DB group than in the SB and LWP groups. In addition, intake of cholesterol per 1,000 kcal was significantly higher in the DB group than in the SB and LWP groups. Thus, SB and DB students seemed to have more night time eating problems than LWP students. Accordingly, nutritional education is needed to support the development of healthier eating habits, in particular, night time eating habits, among students living in dormitories and in self-boarding situations.

The Selection of Appropriate Sampler for the Assessment of Macrobenthos Community in Saemangeum, the West Coast of Korea (새만금 외해역에서 대형 저서동물 군집 조사를 위한 적정 채집기의 선택)

  • 유재원;김창수;박미라;이형곤;이재학;홍재상
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.8 no.3
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    • pp.285-294
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    • 2003
  • To select an appropriate sampler for the environmental monitoring survey in coastal waters of Saemangeum, Jeollabuk-do, a macrobenthic sampling was conducted in April 2002. Employed samplers were dredge (type Charcot), a semi-quantitative sampler and Smith-McIntyre (SM) and van Veen grab (VV) as quantitative ones. One haul was tried for dredge and 3 replicates (0.1 ㎡${\times}$3) for SM and W at each of 11 stations. Comparisons of sediment volume in sampler bucket and of precision of biological parameters (i.e., density, biomass, species number and diversity index, H') were made between SM and VV. Sediment volume was significantly different (SM > VV) at p-value of 0.0050 (paired t-test) and, in average, 3 replicate samples of SM and VV satisfied a precision level of 0.2 by applying 4th root transformation. Patterns of observed and expected species numbers and H' were compared. Dredge-VV samples showed higher affinity than any other pair. Several dominant species in the area were underestimated in dredge samples (e.g., polychaete Heteromastus filiformis. Aricidea assimilis etc.). Quantifying the agreement pattern of multi-species responses was accomplished by estimating correlations between similarity matrices. Correlation between dredge and VV was slightly higher, but near-per-fect matches were found in general. Different ranks and composition among principal species lists were presumably linked to the effect of penetration depth that differs among samplers. Lower level of some species' abundance in VV samples (ca. 50% compared with those of SM) was explained in this context. It seem appropriate to regard the effect as a probable cause of relatively higher correlations in dredge-VV, Overall bio-logica1 features indicated that a better choice could be SM in situations of requiring high data quality. The others work well, however, on observing and defining faunal characteristics and their capability cannot be questionted if we do not expect a first-order quality.

Prevalence and Risk Factors of the Gallbladder Polyps Diagnosed by Ultrasound (복부초음파검사로 진단된 담낭용종의 유병률과 위험인자 분석)

  • LEE, Mi-Hwa;Cho, Pyong-Kon;Kwon, Duck-Moon
    • Journal of radiological science and technology
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    • v.38 no.2
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    • pp.127-134
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    • 2015
  • This study was designed to determine the prevalence of gallstones in the last three years and evaluate the associated risk factors in the population who underwent health screening. Although there are many studies reporting the prevalence and risk factors of GB polyp, the results varied among each report. The aims of this study were to evaluate the prevalence rate and risk factors of GB polyp, colon polyp and fatty liver in the population who underwent health screening. The study population consisted of 4,877 visited the health promotion center in Dalseogu, Daegu in Korea from January 2011 to December 2013. Each participant in the study had their biliary system gallbladder examined using ultrasonography. The prevalence of GB polyp was evaluated along with age, gender, metabolic syndrom, body mass index (BMI), Fatty liver, Colon polyp. A showed of total 383 (7.9%) people were found to have GB polyps. The prevalence of sex among 256 (9.8%) patients men and 127 (5.6%) women which showed significantly higher in male than in female subjects(p=0.001). The mean size of the GB polyps 4.92 mm (1.6-17 mm). The sizes of most GB polyps (73.6%) were less than 10 mm in diameter. 122 subjects (31.28%) had multiple GB polyps which 2 or more polyps and 261 subjects (68.2%) had single polyp. Independent risk factors related with GB polyp were male gender (OR 0.551, p<0.001), overweight that BMI above $23kg/m^2$ (OR 0.713, p=0.002) triglyceride (OR 0.571, P<0.001), metabolic syndrome (OR 0.049, p=0.033) and colon polyp (OR 1.409, p=0.002). In spite of the conclusion, the prevalence GB polyp was higher than previous Korea and other country reports. The GB polyp in a healthy population was results as 7.9%. The risk factors of GB polyps were found to be male, being overweight, triglyceride, metabolic syndrome and colon polyp. Not only the subject of a health examination is needed but, a further study of the general public when possible.

A Study of Coloration of Topaz(I): Mineralogical and Chemical Study on the Topaz Selected from Some Localities of the World (토파즈의 人工着色 處理를 위한 硏究(I) : 世界 主要 産地別 토파즈의 鑛物學的 및 化學的 特性)

  • Han, Yi-Kyeong;Park, Maeng-Eon;Jang, Yong-Nam
    • Journal of the Mineralogical Society of Korea
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    • v.5 no.2
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    • pp.109-121
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    • 1992
  • For the purpose of mineralogical and chemical study on the topazes from various localities of world(Brzail, China, India, Nigeria and Sri Lanka), electron microprobe analysis(EPMA), neutron activation analysis(NAA), X-ray diffractometry, Raman spectroscopy, etch test, scanning electron microscopy, refractive index, specific gravity, fluid inclusion were performed. The chemical composition in topaz was discussed along with its physical and structural properties. Variations in the unit-cell dimension and physical properties of topaz were found to have a close relations in the unit-cell dimension and physical properties of topaz were found to have a close relationship with extent of substitution of $OH^-\;for\;F^-$. According to neutron activation analyses, the trace elements had no effects on the physical properties of topaz. Raman spectra showed that the peaks of topaz were different in intensity from one locality to another. Etching defects in topaz includes negative crystal defect o point-bottom pit(India, Nigeria) and net work defect of curl-bottom pit(Brazil, China). Fluid inclusions in topaz may be classiffied into liquid $CO_2$-bearing inclusion, gaseous inclusion, halite, sylvite-bearing inclusion and liquid inclusion. The results of this study can be useful to devising artificial coloring methods for topaz with different mineralogical compositions.

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Formation of Clay Minerals by Water-Rock Interaction in the Fracture of Gneiss (편마암 열극에서의 물-암석 상호반응에 의한 점토광물 생성)

  • Jeong, Chan-Ho;Kim, Soo-Jin;Koh, Yong-Kwon
    • Journal of the Mineralogical Society of Korea
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    • v.7 no.1
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    • pp.49-61
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    • 1994
  • As the groundwater flows along the fractures of crystalline rocks, it will be in contact with the fracture walls mostly coated by secondary minerals which are quite different form those of host rocks. The presence of fracture-filling minerals in crystalline rocks is important on the view point of radioactive waste disposal because of their great surface reactivity. The Surichi drill hole of 200 m in depth in the Yugu area composed mainly of Precambrian gneiss was selected to study the formation process of clay minerals on the fracture wall of gneiss, and their relation with present groundwater. The water-rock interaction in fractures resulted in the formation of gibbsite and clay minerals. They are formed by two different processes : (1) Incongruent dissolution of feldspar by groundwater diffused from a fracture path into rock matrix produced smectite and illite in situ, (2) on the wall of fracture, gibbsite, kaolinite, smectite and illite are formed by precipitation of dissolved species in groundwater. They show the paragenetic sequence such as gibbsite${\leftrightarrow}$kaolinite${\leftrightarrow}$smectite or illite. The paragenetic sequence of fracture-filling minerals was controlled by increase of pH of groundwater, decrease of fracture permeability by precipitation of fillings, and immobility of alkali or alkaline earths in groundwater. The groundwater from the Surichi borehole is a $Na-HCO_{3}$ type with pH range of 8.6-9.2. The sodium and bicarbonate in groundwater would be supplied by the dissolution of albite and calcite, respectively. The saturation index of groundwater and surface water calculated by WATEQ4F indicates that gibbsite and kaolinite are under precipitation to equilibrium state, and that smectite and illite are under equilibrium to redissolution environment. The stability relation of clay minerals in the $Na_{2}O-Al_{2}O_{3}-SiO_{2}-H_{2}O$ system shows that kaolinite is stable for all waters.

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