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Starter culture production of Rhodospirillum rubrum P17 for use in treatment of organic waste water (유기폐수처리를 위한 Rhodospirillum rubrum P17의 종균생산)

  • Cho, Kyung-Dug;Kang, Seong-Og;Lim, Wang-Jin;Cho, Hong-Yon;Yang, Han-Chul
    • Applied Biological Chemistry
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    • v.36 no.6
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    • pp.488-494
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    • 1993
  • A photosynthetic bacterium strain P17 having high growth rate and assimilating ability of organic acids was isolated from several soil samples, which was identified as Rhodospirillum rubrum. Cultural conditions of the strain P17 were examined for the production of starter culture used in the treatment of organic waste water. The addition of organic acids mixture as carbon source containing 0.2% Na-acetate, 0.1% Na-propionate and 0.2% Na-lactate and 0.1% of yeast extract as growth factor stimulated the cell growth. The maximal cell production was obtained at $30^{\circ}C$, pH 7.0, 2,500 lux of illumination and $50{\sim}100\;rpm$ of agitation. Under the optimal conditions of batch and fed-batch culture systems in a Jar fermentor, 5.17 g/l and 7.93 g/l of cells were obtained after S days of cultivation, respectively. In continuous culture system, the cell productivity was 0.206 g/l/h at a dilution rate of 0.21 $h^{-1}$. When R. rubrum P17 was cultivated in a soybean curd waste water, initial COD level(3,240 mg/l) of the waste water was reduced to 250 mg/l after 4 days of cultivation.

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Flowering Control by Using Red Light of Chrysanthemum (적색광을 이용한 국화의 개화조절)

  • Hong, Seung-Chang;Kwon, Soon-Ik;Kim, Min-Kyeong;Chae, Mi-Jin;Jung, Goo-Bok;So, Kyu-Ho
    • Korean Journal of Environmental Agriculture
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    • v.32 no.2
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    • pp.123-127
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    • 2013
  • BACKGROUND: The incandescent bulb and compact fluorescent lamp are widely using as a light sources for daylength extension of chrysanthemum. But, these light sources consume a lot of electricity and have short longevity. A light-emitting diode (LED) is a semi conductor light source. LEDs have many advantages over incandescent light sources including lower energy consumption, longer lifetime. In this study, we investigated the intensity of red light to control flowering of chrysanthemum (Dendranthema grandiflorum cv. "Shinma") by using LEDs. METHODS AND RESULTS: The red (660 nm) and far-red (730 nm) light were irradiated subsequently to investigate photo-reversible flowering responses of chrysanthemum. The flowering of chrysanthemum was inhibited by night interruption with red light but subsequently irradiated far-red light induced the flowering of chrysanthemum. This photoreversibility, reversion of the inductive effect of a brief red light pulse by a subsequent far-red light pulse, is a property of photo responses regulated by the plant photoreceptor phytochrome B. Four different intensity of red light of 0.7, 1.4, 2.1, and $2.8{\mu}mol/m^2/s$ (PAR) were irradiated at growth room in order to determine the threshold for floral inhibition of chrysanthemum. Over $1.4{\mu}mol/m^2/s$ of the red lights irradiated chrysanthemums were not flowered. The plant length, fresh weight, number of leaves, and leaf area of chrysanthemum irradiated with red light were increased by 17%, 36%, 11%, and 48%, respectively, compared to those of compact fluorescent lamp. CONCLUSION(S): The red light and subsequential far-red light showed that the photoreversibility on flowering of chrysanthemum. The red light ($1.4{\mu}mol/m^2/s$ of red LEDs) and white light (50 Lux of compact fluorescent lamp) have the same effect on inhibition of flowering in chrysanthemum. Additionally, the red light increased the plant height and dry weight of chrysanthemum.

A Study on the Improvement of Aquaculture Security System to Insure the Lawful Evidence of Theft (도적행위의 법적증거확보를 위한 양식장 보안 시스템 개선에 관한 연구)

  • Yim, Jeong-Bin;Nam, Taek-Keun
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.13 no.4
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    • pp.55-63
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    • 2007
  • The Group Digital Surveillance System for Fishery Safety and Security (GDSS-F2S) is to provide the target tracking information and the target identification information in order to secure an huge aquaculture farm-field from a thief. The two information, however, is not enough to indict the thief due to the lack of lawful evidences for the crime actions. To overcome this problem, we consider the target image information as one of solutions after discussion with the effective countermeasure tools for the crime actions with scenario-based analysis according to the geological feature of aquaculture farm-field. To capture the real-time image for the trespassing targets in the aquaculture farm-field area, we developed the image capture system which is consists of ultra sensitive CCD(Charge-Coupled Device) camera with 0.0001 Lux and supplementary devices. As results from the field tests for GDSS-F2S with image capture system, the high definite images of the vehicle number plate and shape, person's actions and features are obtainable not only day time but also very dark night without moon light. Thus it is cleary known that the improved GDSS-F2S with image capture system can provide much enough lawful evidences for the crime actions of targets.

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The Effect of The Index of Indoor Environment on The Productivity (실내환경지수가 생산성에 미치는 영향)

  • Kim, Myung-Ho;Lee, Ye-Jin
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.1
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    • pp.615-621
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    • 2018
  • To enhance the energy saving and comfort of indoors, this study performed a stimulation of sound fluctuation, color temperature, and aroma. The experiment with EEG, HRV, and Vibra images was conducted in an environmental test room with a temperature of $25[^{\circ}C]$, relative humidity of 50[RH%], air current speed of 0.002[m/s], and illuminance of 1000[lux]. The stimulation experiment set up different sensory stimulation conditions, such as before exposure, single-sensory stimulation of fluctuation a=1.106 jazz music, single-sensory stimulation of RED color lighting, single-sensory stimulation of scent aroma, and multi-sensory stimulation of fluctuation a=1.106 jazz music, RED color lighting, and scent aroma. After the multi-sensory stimulation of fluctuation a=1.106 jazz music, RED color lighting and scent aroma, the capacity for work and attention were increased, and the stress index and fatigue degree were decreased. In addition, multi-sensory stimulation of fluctuation a=1.106 jazz music, RED color lighting, and scent aroma were effective in maintaining a stable heart and health. In addition, the Vibra image appeared to decrease tension/anxiety and stress. The multi-sensory stimulation of fluctuation a=1.106 jazz music, RED color lighting, and scent aroma help increase the Neuro-energy more than that by no exposure and single-sensory stimulation.

Fruit-body Formation of Flammulina velutipes on the Synthetic Medium -Part 3. Environmental Characteristics- (합성배지(合成培地)를 이용(利用)한 팽나무버섯의 자실체(子實體) 형성(形成) -제삼보(第三報). 환경적(環境的) 특성(特性)에 관하여-)

  • Kim, Hyong-Soo;Hong, Jai-Sik;Kim, Myung-Kon
    • Applied Biological Chemistry
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    • v.27 no.1
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    • pp.34-39
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    • 1984
  • Environmental effects of light. temperature, and pH of culture media on mycelial growth and fruit-body formation of Flammulina velutipes in synthetic media were investigated. Illumination inhibited mycelial growth, but illumination during the latter part of vegetative growth induced primordia formation. The optimum light intensity and exposure time were $100{\sim}1000$ lux and $4{\sim}8$ hours per day, respectively. High intensity of light was injurous, and in darkness primordia developed into very poor fruit-bodies. The optimum temperature was $25^{\circ}C for mycelial growth and $15^{\circ}C$ for fruit-body formation. The optimum pH range for mycelial growth was found to be from 5.0 to 7.0 and for fruit-body formation from 5.0 to 6.0. In low temperature treatments, a temperature of $15^{\circ}C$ was more effective than $5^{\circ}C\;or\;10^{\circ}C$, it took about 12 hours for primordium formation, but at $5^{\circ}C\;or\;10^{\circ}C$ about 48 hours. The most excellent fruit-body formation were produced from the mycelium growth for 7 to 10 days.

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Optimum Culture Environment of the Benthic Copepod, Tigriopus japonicus (부착성요각류 Tigriopus japonicus의 최적배양환경)

  • PARK Huem Gi;HUR Sung Bum
    • Journal of Aquaculture
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    • v.6 no.3
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    • pp.147-157
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    • 1993
  • The harpacticoid copepod, Tigriopus japonicus is one of the most important zooplankton as a live food for the production of marine fish fry. Thus, the salinity tolerance and the optimum culture environment of this copepod in terms of salinity, temperature and light were examined. The food values of 6 kinds of phytoplankters and 2 kinds of yeast were also investigated for mass culture of this copepod. The results are as follows: After 5 day culture in the experiment of salinity tolerance, the survival rates of the gravid female at $0\%\;and\;90%o\;were\;40\%\;and\;70\%$, respectively. However, at salinity ranging from $2\%o\;to\;80\%o$, high survival rates above $85\%$ were observed. It means T. japonicus is very euryhalinous species. Temperature was more important factor than salinity for the fecundity of T. japonicus. The optimum culture conditions of this species were $24^{\circ}C,\;24\%o$, and 3,000 lux with 24 L: 0D. Under these culture conditions, the average fecundity from a gravid female per spawning was 38 nauplii, and the interval time between spawnings were 2.05 days. Phaeodactylum tircornutum seemed to be the most suitable phytoplankton as a live food for T. japonicus, and the large chlorophyta, Tetraselmis suecica showed the lowest food value among 6 phytoplankters and 2 yeasts. The food value of w-yeast was better than that of baker's yeast, and it is similar to that of phytoplankton such as Amphora sp., Chlorella ellipsoidea and Nannochloris oculata. So, the w-yeast seems to be appropriate food source for mass culture of T. japonicus.

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MORPHOLOGICAL VIEW ON BIG INDIVIDUALS APPEARED IN THE SAME AGE GROUP OF ZOEA LARVA, MACROBRACHIUM JAPONICUM (DE HAAN) (담수산 새우 Macrobrachium japonicum (De Haan)의 Zoea 유생기에 출현하는 동일영기군 속의 개체변이체에 대한 형태학적 고찰)

  • KWON Chin Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.7 no.3
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    • pp.126-144
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    • 1974
  • Adult female prawn, Macrobrachium japonicum, which used for this work were collected at the river Simanto, Shikoku in Japan, and removed in the laboratory. Newly hatched larvae from an adult reared in circulation-filtered aquaria, which is controlled under the conditions of water temperature $26\pm0.3^{\circ}C$, chlorinity $6.21-6.45\%$ Cl, pH 8.0-8.1, illumination 3000 lux, and fed on Artemia salina nauplius sufficielltly For a period of rearing of zoea larvae, big individuals-individual varying bodies, comparing with standard bodies in the same aged individual group, are occasionally appeared from fifth zoea larva stage, and occurence of it be tempted to relate with the factor of trophic condition. This paper was dealt with a comparative morphological view on big individuals, comparing with standard type in the same aged group, to solve the problem on (1). the existence or nonexistence of stages which it is easier to occurence of big individuals, (2). the rate of development in several appendages of an individual, and (3). a happening of skipping whether it certainly occur or not, during newly hatched zoea larvae develop to post larva stage. The results of the above are as follow: (1). the stages which is easier to occur of big individuals are fifth and seventh stage in this species, (2). even same individual, development of several appendages differs more or less on the rate of growth in accordance with its parts, (3). Evidence which skipping phenomenon is occured, during development through zoea larvae to post larvae, couldn't confirm.

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DEVELOPMENT, GROWTH, AND RELATIONSHIP BETWEEN TEMPERATURE AND METAMORPHOSIS RATE OF THE EARLIER LARVAE ON MACROBRACHIUM ROSENBERGI (DE MAAN) (Macrobrachium rosenbergi (De Maan)의 초기유생의 성장 및 수온과 변태와의 관계)

  • KWON Chin Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.7 no.2
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    • pp.52-62
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    • 1974
  • The fresh water prawn, Macrobrachium rosenbergi (De Maan) is a very common species in Info-pacific region inhabiting both fresh and brackish waters in low land areas, and especially abundant in the lower reaches of most rivers which are influenced by seawater, It is one of the largest and commercial species of genus Macrobrachium. As a place of the researches to clear the possibilities of transplantation and propagation of this species in the Far East region the larval development, growth, optimum temperature and metamorphose rate up to first post larvae in aquaruim are cleared under the conditions of salinity $6.58-7.05\%_{\circ}$ Cl, pH 8.0-8.2, the rate of flow 0.3 liter per minute and illumination 3000 lux. Temperature ranged from 27.5 to $28.7^{\circ}C$ during the period of earlier larval development. For the study oil the relationship between temperature and metamorphose rate from zoea to first post larvae, the temperatures in experimental tank were $22.2^{\circ}C\pm1$, $26.1^{\circ}C\pm0.85$, $28.1^{\circ}C\pm0.34$, $30.4^{\circ}C\pm0.66$, $33.7^{\circ}C\pm0.66$, $33.7^{\circ}C\pm0.38$ and $36.8^{\circ}C\pm0.26$. During the work, food used for the larvae was Artemia salina nauplius in the filter-circulation aquariums. This species metamorphosed to the first post-larvae through eleven zoea stages, and the characters of each larval stage are described and optimum temperature for metamorphosis rate and survivals to the first post larvae is $28.1^{\circ}C\pm0.34$.

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The Study about Improvement of Neuro Energy Decreased by Energy Saving (에너지절감에 의해 감소되는 뉴로에너지의 증강에 관한 연구)

  • Kim, Myung-Ho;Kang, Dong-Sik
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.6
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    • pp.715-721
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    • 2018
  • This study examined energy saving and elevating the worker's neuro energy (comfort, concentration, physical, and psychological stability) by compensating for the unpleasant tactile sensation to stimulate auditory and olfactory senses and reduce energy consumption. The experiment was conducted in an environment test room under the test conditions of temperature $25[^{\circ}C]$, relative humidity 50[RH%], illumination 1,000[lux] and air current speed 0.02[m/sec] by stimulating the auditory senses with a 1/f change in rhythm and the olfactory senses with an aroma scent. The experiment utilized the method of EEG, which evaluates human body's psychological status via tactile means, and the method of the vibra image, which evaluates the learning abilities, HRV and human body's psychological status via non-tactile means. The subjects were selected as eight university students (four males and four females) in their 20s, the type that have high relative ${\alpha}$(8~13[Hz]) activation in occipital lobe, which brings the highest level of mind stability and concentration, who had no difficulty in physical activities. The subjects' posture and physical activity was fixed to 1met - when the subjects are seated and relaxing in a comfortable environment - and their clothes condition was standardized as 0.7clo. As a result, the sentimental and psychological stability and concentration were the highest in the multisensory stimulation of jasmine scent and change rhythm of an a=1.106 sound source. In addition, under this condition, the relative $M{\alpha}$ and relative $M{\beta}$ increased by 70.49[%] and 89.72[%], respectively; the HRT decreased by 39.09[%]; and the fatigue and tension/anxiety decreased by 36.85[%] and 15.54[%], respectively.

A Study on Selection of SO2 Resistant Tree Species - I. Leaf Disk Experiment - (SO2에 대한 내성수종(耐性樹種)의 선발(選拔)을 위한 기초연구(基礎研究) - I. 엽조직(葉組織) 실험(實驗) -)

  • Kim, Gab Tae
    • Journal of Korean Society of Forest Science
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    • v.77 no.2
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    • pp.223-228
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    • 1988
  • To select $SO_2$-resistant tree species, leaf disks of 6mm in diameter, cut from the leaves of 6 species (Wistaria floribunda, Magnolia obovata, Rosa multiflora, Liriodendron tulipifera, Robinia pseudo-acacia and Acer palmatum) were floated on 25ml of testing medium and placed on laboratory under fluorescent lamp (1,500 Lux) for 20 hours. Chlorophyll content and acidity of the testing medium were measured. Testing medium was prepared by diluting $H_2SO_4$, $H_2SO_3$ and $Na_2SO_4$ with distilled water for various stoichiometric $SO_2$ concentrations, 0, 25, 50, 100 and 250 ppm. Total chlorophyll content was more decreased after treatment than before treatment, and was decreased more severely in $H_2SO_3$ sources, followed by $H_2SO_4$ and $Na_2SO_4$, sources. Decreasing rate of total chlorophyll content was generally large in Acer palmatum. Magnolia obovata and Wistaria floribunda, and was relatively small in Rosa multiflora, Liriodendron tulipifera and Robinia pseudo-acacia. Decreasing rate of chlorophyll content may be useful index for judging susceptifility of the leaf to $SO_2$. The acidity of the testing medium was generally decreased after treatment, and it means that cell leakage was occurred during treatment. The differences in medium acidity between before and after treatment may be poot index for susceptibility of the leaf to $SO_2$ owing to the difference among tree species in development of leaf mesophyll, acidity maintaining mechanism and butter capacity of the leaf tissue.

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