• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
• /
• 1998.10a
• /
• pp.166-174
• /
• 1998

The distribution of oil film thickness in piston were measured by induced fluorescence method. A Xe lamp was used as light source. Coumarine-6 was mixed with oil as the fluorescent dye. Fluorescent signal which is proportional to the oil film thickness was acquired by CCD camera and transmitted to the personal computer as video signal. In order to solve the problem of measurement system, irregular distribution and unstability of light intensity, as well as to know the relationship between the oil film thickness and output signal, three different calibration techniques were used. Motoring and firing tests were performed in a single cylinder research engine with transparent liner. By analyzing the oil film thickness converted from the photographed image, it was observed that each of three piston rings scrapes the oil both upward and downward and oil film thickness is not uniform horizontally at a given piston land. The amount of oil in each land was considerably affected by the engine load. It is thought that the blow-by gas blows the oil down to the crankcase.

• The Korean Journal of Zoology
• /
• v.34 no.1
• /
• pp.44-53
• /
• 1991

Distribution of the antigens during the cell cycle of amoebae was followed by immunof-luorescence microscopy using a monoclonal antibody against the nucleus as a probe. While the cells were in the interphase, the antigen was localized on the nucleus membrane. But it was dispersed all over the cytoplasm during mitosis and cytokinesis. The molecular weights of the immunoreacted antigens were 210 KD and 190 KD as determined by SDS PAGE and western blotting of the purified nuclei. The antigens were not soluble in non-ionic detergent, but were released from the nucleus by incubation with 0.05 M sodium carbonate, pH 10.6 or with 8 M urea at serial chemical extraction. Thus the antigens appeared to be peripheral proteins of the nurBeus envelope. The isoelectic point of both antigens was 7.64 as determined by 2 D PAGE and transfer blotting. Considering the peiipherd association with the nucleus membrane and the dispersed distribution during mitosis, the antigens could be lamin like proteins. Hourever, it appears also possible that they are the component molecules of the unusually structured aurous lamina of amoeba nucleus since they have the large molecular weight and the basic pl.