• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.5
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• pp.533-542
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• 2011

Numerous seagrass habitat restoration projects have been attempted recently due to the remarkable decline in seagrass coverage. Seagrass transplants tend to adapt to a new environment after experiencing transplanting stress during the early stages of transplantation. Once acclimated, the transplants grow into healthy seagrass beds via vegetative propagation. The establishment and growth dynamics of transplanted seagrasses in bays and coasts are widely reported, but few studies have been conducted on estuaries in Korea. We transplanted Zostera marina in November 2007 and November 2008 in the Nakdong estuary using the staple method, and monitored the survival, adaptation, and growth dynamics of the transplants as well as environmental factors every month for 1 year. Both transplants adapted well to the new environment without initial losses and showed rapid productivity during early summer. However, density of transplants increased 320% in 1 year from the previous year's transplants but that decreased to 59% during the following year. This significant reduction in density in the second year may have been caused by exposure to low salinity (10 psu) for 3 weeks during the unusually long monsoon season. While the survival and growth dynamics of seagrass transplants planted in bays and coasts are mainly controlled by underwater photon flux density and water temperature, salinity was the critical factor for those planted in Nakdong estuary.

• Korean Journal of Environmental Biology
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• v.30 no.1
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• pp.47-53
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• 2012

To evaluate adaptation success of $Zostera$ $caespitosa$ transplants, we transplanted the seagrass shoots at the bare area in close proximity to the donor bed using staple method in October 2005. Shoot density, morphological characteristics and leaf productivity of transplants and reference plants in the vicinity of the planting site were monitored monthly for 2 years. While shoot density of reference plants exhibited significant seasonal variations; increasing during spring and summer and decreasing during fall and winter, that of transplants increased consistently without initial loss during the whole study period. Although sheath length, leaf width and shoot height and weight of sheath, leaf and shoot of transplants were smaller than those of reference plants at the start of transplantation, increased rapidly reaching even higher values than those of reference plants 5 months after transplantation. Leaf productivity of transplants and reference plants showed seasonal variations; increasing during spring and summer and decreasing during fall and winter. But, leaf productivity of transplants increased at the beginning of transplantation during fall which is low production period. All of the $Z.$ $caespitosa$ transplants survived during the whole study period. Rapid changes in shoot morphology and growth of transplants indicated that $Z.$ $caespitosa$ transplants had great morphological plasticity and adapted successfully within 5 months.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.2
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• pp.112-119
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• 2021

In order to develop a safe seeding technology for low-density transplantation, we analyzed the changes in seeding growth according to different seeding rates and seeding days. We detected changes in leaf number and shoot diameter on the 10th seeding day, and in plant height on the 20th seeding day according to seeding rate. The increase in shoot and leaf number, and shoot diameter per seeding day decreased with anincrease in seeding rate. On seeding day 20, the reductions in shoot and root dry weights according to seeding rate was lower compared with those recorded at seeding day 10. Shoot dry weight was significantly related to root dry weight, plant height, and shoot diameter. With an increase in seeding rate, seeding health decreased and there was an increase in the rate of seeding health per seeding day decreased. For low-density transplantation, the optimal number of seeds required for transplanting 3-5 plants per hill is 11,000, which is estimated to be approximately 280-300 g of seeds for 22 g brown rice. Furthermore, the appropriate number of seeding days is estimated to be 13 to 16 days, which corresponds to a small reduction in seeding health.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.167-167
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• 2022

Seed production of italian ryegrass has a problem of lodging during ripening and a decrease in quality due to difficulty in drying seeds during harvest. Therefore, in order to produce high-quality Italian ryegrass in paddy fields, it was carried out to reduce the density and solve the lodging problem through transplanting. In this experiment, Lolium multiflorum cv. Kowinearly was transplanted in autumn from a paddy field in Sindong-ri, Gwansan-eup, Jangheung-gun, Jeollanam-do. var. Kowinearly was made into a bed at 90 g/box and stacked in boxes. It was transplanted on October 27th after 2 days of germination at 30℃ and 15 days of seedling and greening. When transplanting, they were transplanted at intervals of 30×14cm. The existing cultivation method, drill seedling, was sowed at a level of 50 kg/ha, and both transplanting and drilling were carried out at a nitrogen fertilization rate of 45 kg/ha. The number of ears during transplant cultivation was 1,016/m² and the drilling tended to be higher at 2,278/m², but this was probably due to the difference in seeding amount. The seed number of an ear tended to be 56% higher in transplantation, which had a significant impact on yield. The seed yield was 2,096 ka/ha in transplantation, which was 21% higher than that of drilling. When looking at the relationship with weed occurrence, there were areas where all the weeds, such as amul foxtail, occurred due to the low density. Even in the same transplanting area, the seed yield was about 1,000kg/ha less in the area where the weeds were abundant. It seems that weed management is important in paddy cultivation. Therefore, it seems necessary to develop an exclusive herbicide for Italian ryegrass cultivation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.4
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• pp.279-288
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• 2021

Low-density transplanting is a cultivation technology that reduces labor and production costs. In this study, the growth and yield of several varieties with different tillering characteristics were analyzed in order to establish an appropriate planting density for low-density transplanting. Varieties with Low-Tillering (LT), Medium-Tillering (MT), and High-Tillering (HT) were planted at a density of 37-80 hills/3.3 m². As the planting density decreased, the number of tillers per hill increased, but the number of tillers per square meter of hill decreased, especially for the LT variety. Decreasing density extended the tillering stage, which was longest in the LT variety. As the planting density decreased, SPAD(Soil plant analysis development, chlorophyll meter) values just before heading increased while canopy light interception decreased. Such changes were much greater in the LT variety than in the MT and HT varieties. The heading date tended to be delayed by 0-2 days as the planting density decreased, and there was no difference in the length of the period from first heading to full heading. As the number of spikelets per panicle increased, the number of spikelets per square meter did not differ according to the planting density. Decreasing planting density did not affect the grain weight; nevertheless, the yield ultimately decreased because of the decreasing ripening rate. The optimal planting density for stable low-density transplanting cultivation was determined to be over 50 hills/3.3 m2. In addition, these results suggest that LT varieties should be avoided, since these showed large decreases in growth and yield with decreasing planting density.

• Journal of Yeungnam Medical Science
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• v.32 no.2
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• pp.155-158
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• 2015

Lymphangioma is a congenital abnormality of the lymphatic system detected primarily in early childhood. There are rare reports of mediastinal lymphangioma in older adults. We hereby report on a 66-year-old female patient who underwent kidney transplantation 20 years previously and who developed pathologically confirmed solitary mediastinal lymphangioma 1 year ago. Chest radiography showed a mediastinal nodule, which was not observed 2 year previously, therefore she was referred to the pulmonary division. She had no symptoms, and chest computed tomography demonstrated a 25-mm, well-defined, low-density nodule located at the anterior mediastinum. The size of the nodule had increased from 25 mm to 34 mm 1 year later, and it was completely resected via video-assisted thoracic surgery. The histological diagnosis was cystic lymphangioma. Therefore, we recommend that clinicians consider cystic lymphangioma as a possible diagnosis even in older patients with a mediastinal cystic mass that shows progressive enlargement.

• Journal of Aquaculture
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• v.13 no.4
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• pp.339-351
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• 2000

Optimal environmental conditions, that sustained fastest growth, lowest mortality and abnormality of the scallop Patinopecten yessoensis, were identified from field experiments undertaken at Chumunjin during 1991-1998. Temperature within the water column 10~30 m depth ranged between 5 and 23$^{\circ}C$; high temperature and daily fluctuation resulted in growth retardation and heavy mortality of the scallop. Optimal salinity range was between 31.5 and 34.5%0 and water transparency 6.0 and 18.1 m, which was significantly affected by phytoplankton density. Chlorophyll concentration ranged between 0.04 and 3.51 f.lgfL. Low temperature and high chlorophyll concentration appear to support faster growth of the scallop. Optimal periods of transplantation for intermediate culture were between mid July and early November: cultured under high density during July-August as a first step and under low density during mid September through early November as a second step. Optimal stocking density in square net cage (<35${\times}$35 em) for intermediate culture was 30-40 individuals per cage for main culture using lantern net and 80 -100 individuals of the size of 1.5 ~ 3.0 em shell height per cage for sowing culture. During the intermediate culture, the highest growth was realized, when the cage was held at water depth between 10 and 15 m. Water depth below 25 m, however, was best to avoid mass mortality during the periods of abnormally high water temperature and high variation of water temperature. The daily growth rate during the intermediate culture was between 0.019~0.381 mm; low in January and February but high in March and April. It is suggested that the main culture is commenced before June under low stocking density to avoid the possibility of mass mortality during summer by high water temperature.

• Korean Journal of Agricultural Science
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• v.11 no.1
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• pp.68-76
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• 1984

The study was conducted to investigate an optimum growing condition for various sizes of seedlings of rice for mechanical transplantation under different sowing rates, different levels of fertilizers, different sowing dates and g rowing days. The plant height, leaf number and dry weight of seedlings increased as the sowing rate decreased and amount of fertilizer applied were increased. Also those seedling characters increased as the rice planted late and the total growing periods increased. The factors affected seedling chracters were ordered as following-sowing times>growing days>sowing rate>fertilizer levels. 200 gr. of seeds per box and 4 gr. of fertilizers were optimum for young seedling growth, when sown early. For medium size of seed ling growth, 50 grams and 100 to 125 grams of rice seemed to be adequate for 25 days old and 35 days old seedling, respectively. However, the 45 days old seedling grew too mach and were not suitable for mechanical transplantation. When planted late, similar results were obtained with more differences among treatments. In other words, the suitable plant density was obtained when 100 gr. of seeds were planted per box. Middle class of seedlings were obtained when 100 to 150 gr. of rice were planted per box and grown for 25 days. The seedlings from the box with 100 to 150 gr. of rice per box were over grown and ratio of suitable seedling was also low if they were grown over 35 days.

• Korean Journal of Environmental Agriculture
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• v.20 no.2
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• pp.86-92
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• 2001

The current study was conducted to elucidate the optimum density of free-ranging ducks in a rice-duck farming system in terms of effects on duck behavior, and growth and yield of rice plants. Four paddy fields were used for this experiment, with 6, 9, 12 and 15 birds per plot, respectively. Ducklings at eight days of age were free ranged in experimental paddy plots (4.0 a each) on the 9th day after the transplantation of rice plants. Ducks were kept in the plot for seventy days, at which point rice plants reached the earing stage. Foraging, moving, working, resting, diving and pecking behaviors of the ducks were observed two times during the experimental period. Also, rice plant growth and yield according to the density of ducks per plot were examined. The foraging and moving behavior of free-ranging ducks in paddy fields for 12 hours during the daytime tended to be longer in the 12-bird plot, and working behavior was significantly (P<0.01) longer in the 12-bird plot than in the other three plots. The resting behavior was significantly (P<0.01) higher in the 9- and 15-bird plots than in the 12-bird plot. The frequency of moving behavior for 6 hours during the daytime in the 15-bird plot tended to be lower than that in the other three plots, but this difference was not significant. The amount of diving and pecking behavior in the 9-bird plot was significantly (P<0.05) higher than that in the other three plots, and the number of hills pecked tended to be higher with increasing of duck density. From thirty days after ducks were introduced to the paddy fields, the length of rice plants tended to be significantly (P<0.05) shorter in high free-ranging density plots as compared to low free-ranging density plots. The number of tillers per hill was not affected by the free-ranging density. The culm length of rice plants was significantly (P<0.05) shorter in the 12- and 15-bird plots than in the other two plots, however, the duck free-ranging density did not affect panicle length. The dry weight of the root of rice plants was increased with high free-ranging density, but there was no such increase in the top parts of the rice plants. The percent of rice plants badly damaged by free-ranging density tended to be lower in the order of 12-, 9-, 6- and 15-bird plots. The number of ears, ripening grains and crop yield per hill of rice plants in the 12-bird plot were significantly (P<0.05) higher than those of the other three plots. Therefore, the yield of each rice plant per 10 a was significantly increased in the 12-bird plot.

• Archives of Plastic Surgery
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• v.37 no.5
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• pp.509-518
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• 2010

Purpose: Major drawbacks of conventional bone marrow stromal cells (BSCs) transplantation method are mainly caused by direct transplanted cell to host cell interactions. We hypothesized that separation of the transplanted cells by a microporous membrane might inhibit most of the potential adverse effects and induce superior effect. The purpose of the study is to determine the optimal condition of the microporous membrane. Methods: First, BSCs were placed in polyethylene terephthalate (PET) transwell inserts with 3, 8, or $12{\mu}m$ pore size, and cultured in 24 well culture plates. After 5 days, bottoms of the plates were observed for presence of attached BSCs in monolayer and cell numbers were evaluated. Second, BSCs were placed PET, polycarbonate (PCT), and mixed cellulose esters (MCE) transwell inserts with 3 and $8{\mu}m$ pore size, and cultured in 24 well culture plates. After 3 days, the supernatants of the media left in culture plate were analyzed for collagen, vascular endothelial growth factor (VEGF), platelet derived growth factor BB (PDGF-BB), and basic fibroblast growth factor (bFGF). Third, BSCs were placed in 15% and 70% of the PET membrane with $3{\mu}m$ pore size. All the experimental conditions and methods were same as the second study. Results: The optimal pore sizes to prevent BSC leakage were $3{\mu}m$ and $8{\mu}m$. The amounts of type I collagen and three growth factors tested did not show significant differences among PET, PCT, and MCE groups. However, the collagen, VEGF, and bFGF levels were much higher in the high (70%) density group than in the low (15%) density group. Conclusion: This study revealed that the optimal pore size of membrane to prevent direct BSC to recipient cell contact is in between $3{\mu}m$ and $8{\mu}m$. Membrane materials and pore sizes do not influence the collagen and growth factor passage through the membrane. The most striking factor for collagen and growth factor transport is pore density of the membrane.