• Asian-Australasian Journal of Animal Sciences
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• 제19권6호
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• pp.831-836
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• 2006

This study compared the effects of two levels of crude protein (CP) supplementation during a close-up dry period on the productive performance, nitrogen balance, and blood profiles of Holstein cows. Eighteen cows (8 primigravid and 10 multiparous), 21 days prior to calving, were divided into four groups and fed a diet containing a low (11.5 to 12.7% CP) or high (14.1 to 15.3% CP) level of protein. Following parturition, all cows were offered the same diet. Nitrogen balance was measured 12 d to 10 d prior to the expected calving date. Whole feces and urine were collected. The higher close-up CP did not improve milk production during the early lactation period (14 w) in multiparous cows but tended to increase production by 2 kg of milk per day in primigravid cows. There were no differences in the weight of calves, the weight of the placenta, or the incidence of calving difficulty. Nitrogen intake, urine nitrogen and nitrogen retention were increased by the higher CP diet in primigravid and multiparous cows. All groups of cows showed a positive nitrogen balance. In particular, higher nitrogen retention was observed in primigravid cows fed the higher CP diet. With the exception of plasma urea nitrogen (PUN), non of the blood profiles were influenced by the CP level. The higher close-up CP increased the PUN during the period studied in primigravid and multiparous cows. These results indicate that a CP level of 14% during a close-up dry period is optimal for primigravid cows, and 12% CP level is sufficient for multiparous cows.

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 2016년도 추계학술대회 논문집
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• pp.156-156
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• 2016

The effect of nitrogen doping on the mechanical and tribological performance of single-layer tetrahedral amorphous carbon (ta-C:N) coatings of up to $1{\mu}m$ in thickness was investigated using a custom-made filtered cathode vacuum arc (FCVA). The results obtained revealed that the hardness of the coatings decreased from $65{\pm}4.8GPa$ to $25{\pm}2.4GPa$ with increasing nitrogen gas ratio, which indicates that nitrogen doping occurs through substitution in the $sp^2$ phase. Subsequent AES analysis showed that the N/C ratio in the ta-C:N thick-film coatings ranged from 0.03 to 0.29 and increased with the nitrogen flow rate. Variation in the G-peak positions and I(D)/I(G) ratio exhibit a similar trend. It is concluded from these results that micron-thick ta-C:N films have the potential to be used in a wide range of functional coating applications in electronics. To achieve highly conductive and wear-resistant coatings in system components, the friction and wear performances of the coating were investigated. The tribological behavior of the coating was investigated by sliding an SUJ2 ball over the coating in a ball-on-disk tribo-meter. The experimental results revealed that doping using a high nitrogen gas flow rate improved the wear resistance of the coating, while a low flow rate of 0-10 sccm increased the coefficient of friction (CoF) and wear rate through the generation of hematite (${\alpha}-Fe_2O_3$) phases by tribo-chemical reaction. However, the CoF and wear rate dramatically decreased when the nitrogen flow rate was increased to 30-40 sccm, due to the nitrogen inducing phase transformation that produced a graphite-like structure in the coating. The widths of the wear track and wear scar were also observed to decrease with increasing nitrogen flow rate. Moreover, the G-peaks of the wear scar around the SUJ2 ball on the worn surface increased with increasing nitrogen doping.

• Journal of Applied Biological Chemistry
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• 제65권1호
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• pp.1-6
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• 2022

요소는 가장 보편적인 질소비료 중 하나인데 빠른 가수분해와 환경으로의 질소 손실로 인해 작물에 질소 이용효율이 낮다. 따라서 요소 비료에서 질소 방출을 제어하는 것이 중요하다. 본 연구에서는 토양의 요소가수분해효소를 억제하여 요소에서 과도한 질소 용출을 방지하기 위해 목초액을 처리하였다. 목초액의 유효성분은 목초액을 처리하지 않은 토양에 비해 암모늄태 질소의 추출량을 79.7% 감소시켜 토양에서 요소의 암모니아화를 억제한 것으로 나타났다. 목초액이 요소의 시비 효율에 미치는 영향을 평가하기 위해 요소와 목초액을 처리한 토양에서 배추(Brassica campestris var. Pekinensis)를 각각 포트와 포장에서 재배하였다. 목초액을 요소와 같이 처리했을 때 관행적으로 사용하는 요소량의 반을 사용하였으며 관행과 비교하여 유사한 배추 생체중을 보였다. 배추의 질소 함량은 목초액 처리구에서 낮아 배추가 질소를 효율적으로 사용한 것으로 판단된다. 따라서 요소를 목초액의 유효성분과 같이 처리하면 시비량과 시비 횟수를 감소시킬 수 있다.

• 농업과학연구
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• 제50권2호
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• pp.223-234
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• 2023

Appropriate fertilization methods are required according to species to supply necessary nutrients to plants and prevent soil environmental contamination in nurseries. In this study, the effects of nitrogen and phosphorus fertilization on the growth of Liriodendron tulipifera and soil characteristics were investigated. After 16 fertilization treatments (4 levels of nitrogen × 4 levels of phosphorus) were applied to one-year-old L. tulipifera seedlings at the Yongmun Nursery Station of the Korea Forest Service, height, root collar diameter (RCD), biomass, leaf nutrients, and soil characteristics were investigated. The height increased as the amount of nitrogen and phosphorus fertilization increased, and the RCD was the highest in the ×2 treatment. Biomass growth was on average 40.0% higher for the treatment with high nitrogen fertilization compared to the low nitrogen treatment. The seedling quality index was the highest with nitrogen and phosphorus ×2 treatment. Leaf phosphorus and magnesium concentrations decreased when nitrogen fertilization was applied, and leaf potassium concentrations decreased as nitrogen fertilization increased. Soil pH and exchangeable potassium decreased as the amount of phosphorus application increased, and exchangeable magnesium decreased as the amount of nitrogen application increased and increased as the amount of phosphorus application increased. Considering the growth of L. tulipifera seedlings and changes in the soil characteristics at the nursery stage, twice the standard fertilization amount is the appropriate fertilization amount for nursery of the Yongmun Nursery Station. It is expected that this study will contribute to improving nursery soil fertilization management technology for healthy seedling production.

• 한국지하수토양환경학회지:지하수토양환경
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• 제24권6호
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• pp.26-32
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• 2019

An analysis of groundwater quality is significant for monitoring and managing water contamination and groundwater system. For the purpose of those, the geochemical characteristics of groundwater were studied over the concern for water quality, water type and origin of nitrate nitrogen. Total colony counts were detected in 11 out of 20 samples, and the average value was 31.73 CFU/ml. Range and average of NO₃-N concentrations were 0.9~24.0 mg/L and 8.3 mg/L. All groundwater types were found to be Ca²⁺-HCO₃^-. The range and average of NO₃-N were 0.2~17.4 mg/L and 8.7 mg/L, and those of δ¹⁵N were 1.7~8.9‰, and 5.0‰. Careful consideration is required for evaluating the origin of nitrogen when NO₃-N concentration is low. In general, noticeable difference between rockbed and alluvial water was not found. The ranges of nitrate origins by chemical fertilizer, livestock manure and domestic sewage, and natural soil were 29.6~76.4%, 14.2~58.9% and 2.6~7.0%, and the average values of those were 57.4%, 37.4%, and 5.3%, respectively. Origin of nitrate was affected by more chemical fertilizer than the other parameters. Rockbed water was more affected by chemical fertilizer than alluvial water.

• ALGAE
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• 제19권1호
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• pp.1-6
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• 2004

Carbon ($^{14}CO_2$) and nitrogen ($^{15}NH_4$ and $^{15}NO_3$) uptake were measured at two stations in the hypertrophic zone of the Seonakdong River, where Microcystis aeruginosa explosively bloomed in September 1998. Significant nitrogen limitation occurred in the period of Microcystis bloom, while phosphorus limitation was common in the river. The specific nitrogen ($NH_4$ + $NO_3$) uptake was 12-50 $\mu$mol mg chl-a$^{-1}$ hr$^{-1}$ at two stations, showing substantially higher than for any other freshwaters. The specific nirtogen uptake was higher at the GAR Station of the nitrogen-limited area and this high nirtogen uptake resulted in low $^{14}C:^{15}N$ atomic ratios of algal uptake. Carbon uptake was dependent upon irradiance, decreasing gradually toward the bottom in the euphotic zone, whereas the nitrogen uptake increased slightly toward the bottom. $NH_4$ preferable uptake against $NO_3$ was hardly discemilble due to the fact that it exceeded the $NH_4$ ambient concentraiton. The $^{14}C:^{15}N$ atomic ratios of algal uptake in the surface waters approached the Redfield C:N ratio.

• 한국물환경학회지
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• 제28권1호
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• pp.94-101
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• 2012

The performance data for eight years from a free-surface-flow constructed wetland system receiving agricultural tailwater were used to analyze denitrification rate and nitrogen treatment characteristics according to season and wetland design. Seasonal difference between growing season (March~November) and winter season (December~February) was shown in the concentration of all nitrogen species. Seasonal nitrogen treatment has similar trend with temperature and measured denitrification rate. The highest denitrification rate was measured in July, but treatment efficiency was most higher in May and June. Nitrogen absorption of vegetation could affect to these wetland performances, therefore dense population of wetland vegetation might be helpful. According to design of wetland, at least 25~50 m of wetland length was needed to decrease effluent T-N concentration to background concentration in growing season. In winter season, wetland needed much longer distance to reduce T-N concentration. Mass removal rate was continuously high through whole year because runoff coefficient was low in winter season. Applicability of constructed wetland was observed for the total maximum daily load that control T-N load.

• Journal of Microbiology and Biotechnology
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• 제24권9호
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• pp.1207-1215
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• 2014

Candida albicans, the major human fungal pathogen, undergoes morphological transition from the budding yeast form to filamentous growth in response to nitrogen starvation. In this study, we identified a new function of GST2, whose expression was required for filamentous growth of C. albicans under nitrogen-limiting conditions. The Gst2p showed Gst activity and required response to oxidative stress. The ${\Delta}gst2$ mutant displayed predominantly yeast phase growth in low ammonium media. Such morphological defect of ${\Delta}gst2$ mutants was not rescued by overexpression of Mep2p, Cph1p, or Efg1p, but was rescued by either overexpression of a hyperactive $RAS1^{G13V}$ allele or through exogenous addition of cyclic AMP. In addition, the ${\Delta}gst2$ mutants had lower levels of RAS1 transcripts than wild-type cells under conditions of nitrogen starvation. These results were consistent with the Ras1-cAMP pathway as a possible downstream target of Gst2p. These findings suggest that Gst2p is a significant component of nitrogen starvation-induced filamentation in C. albicans.

• 대한약리학회지
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• 제13권2호
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• pp.67-73
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• 1977

The nitrogen sparing effect of intravenous 3% amino acid solution was compared with 5% dextrose solution in 30 patients who were undergoing surgical operations or radiation therapy. Infusion of 3% amino acid solution or 5% dextrose solution was given before and immediately after operations or irradiation and continued for 6 days. Infusion of solutions through peripheral vein was well tolerated and not experienced any specific hematologic or blood chemistry change in all patients subjected throughout the experiment. The patients received 3% amino acid solution showed low blood glucose and insulin level, but significantly high blood urea nitrogen and ketone body. In patients receiving amino acids, as compared with those receiving dextrose, mean cumulative six day nitrogen losses were significantly lower($63.95{\pm}2.12$ Gm and $79.12{\pm}2.43Gm$ respectively). The nitrogen sparing effect of amino acids is probably due to decreased glucose and insulin levels allowing greater endogenous fat mobilization.

• 한국연초학회지
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• 제25권1호
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• pp.27-33
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• 2003

This study was conducted to determine the changes of nitrogen compounds in lamina and midrib during the curing process with the different curing methods, such as priming and stalk-cut curing. After KB 108 burley tobacco was cultivated by the different fetilization levels such as standard and 20% higher, only the tips and leaf were harvested. Though the contents of total alkaloid and total nitrogen were similar in lamina, midrib showed a very low contents of those components by the different curing method and fertilization levels. The content of nitrate-nitrogen in lamina increased during a middle of curing process, and then these compound was decreased during an end of curing process by stalk-cut curing method. Nitrate-nitrogen contents in the lamina by the priming curing showed a high level caused by rapid drying process during an end of curing process. That component in midrib was 5-6 times higher than that of lamina. The contents of ammonia-nitrogen in the lamina and midrib were increased during a curing process. Though those amount in tips showed a similar by a different fetilization and curing method, midrib of stalk-cut curing showed a slow increasing during a curing process.