• Korean Journal of Veterinary Research
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• v.43 no.3
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• pp.405-414
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• 2003

The purpose of this study was to assess the role of tachykinins (TK) in mediating nonadrenergic noncholinergic (NANC) contractions produced by electrical field stimulation (EFS) in the longitudinal muscle of the rat ileum. In the presence of atropine ($1{\mu}M$), guanethidine ($5{\mu}M$), and L-nitroarginine (L-NNA, $200{\mu}M$), EFS (0.5ms pulse duration, 120 V, 1-20 Hz for 2 min) produced a frequency-dependent slowly-developing tonic contraction with superimposed phasic contractions ('on'-contraction) followed by off slowly-decreasing tonic and superimposed phasic contractions ('off'-contraction) of mucosa-free longitudinal oriented muscle strip. These EFS induced responses were blocked by tetrotoxin. $NK_1$ receptor selective antagonist L-732,138 strongly inhibited the EFS-induced excitatory responses. However $NK_2$ receptor selective antagonist, GR 159897 and $NK_3$ receptor selective antagonist SB 222200 did not significantly inhibited the responses. $NK_1$ receptor selective agonist [$Sar^9$,$Met(O_2)^{11}$] Substance P and $NK_2$ receptor selective agonist [${\beta}-Ala^8$]-neurokinin A (4-10) induced tonic contraction with superimposed phasic contractions of longitudinal oriented muscle strip and almost blocked by selective antagonist L-732,138 and GR 159897, respectively. But $NK_3$ receptor selective agonist senktide did not showed any effect. Nifedipine ($1{\mu}M$) abolished the contraction produced either by EFS or by the TK receptor agonists [$Sar^9$,$Met(O_2)^{11}$] Substance P or [${\beta}-Ala^8$]-neurokinin A (4-10). It is concluded that, in the longitudinal muscle of rat ileum, both $NK_1$ and $NK_2$ receptors modulated the responses to exogenous tachykinins, whereas $NK_1$ is mainly involved in NANC neuromuscular contraction.

• Journal of muscle and joint health
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• v.8 no.2
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• pp.278-286
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• 2001

With the aging of society a great deal of interest is being placed on the value of longitudinal data in evaluating physiological losses. We present data on test-one/test-two reliability and reproducibility for measures of training, bone density from a longitudinal study of master athletes. Fifty-two males (mean age at test $1=58.2{\pm}9.8\;years$) and thirty-two females ($54.4{\pm}8.8\;years$) were selected from the study population. Bone mineral density was determined using DEXA (Hologic 1500). The characteristics of the subjects are presented below as $means\;{\pm}\;S.D$. The data was imported into the Statistical Package for the Social Science (SPSS 9.0, Chicago, IL). Paired t-tests were performed between visit 1 and visit 2 in subjects. Pearson correlations were performed. The results of this study indicate the measures of training history, body mass and bone density are reasonably stable and reproducible. We conclude that body composition and bone density parameters are stable and reproducible over time in active older subjects. Physiologic measures in master athletes are fairly stable, and reproducible over time Longitudinal studies investigating age-related changes in master athletes need to be conducted on a time schedule of greater than two years.

• Journal of Embryo Transfer
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• v.24 no.3
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• pp.159-167
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• 2009

Ultrasonographic examination was performed to observe the ultrasonographic image of Korean native cows' normal uterus in condition of in vitro and in vivo. The experiment was done 28 slaughtered cows' uterus using immersed in water in vitro, and 41 healthy breeding cows taken rectal ultrasonography in vivo. Ultrasonographic examination of uterine was taken on the reference of cross section of intercornual ligaments' cranial. Each uterus on the experiments was compared by estrous cycle and ultrasonographic frequency. The uterine structure using ultrasonography was 5 layers of uterine horn in vivo as well as in vitro. Uterine horn was observed to be distinguished from inside to outside as endometrium to inner echogenic layer, circular muscle layer to slightly echogenic elliptical layer, stratum vasculare to central echogenic layer, longitudinal muscle layer to slightly echogenic arched layer, and perimetrium to outer echogenic layer, respectively. According to the observation of uterus related to estrous cycle and ultrasonographic examination, uterine endometrium in vitro was constantly founded irrespective of estrous cycle and ultrasonographic frequency. On the low frequency, endometrium and circular muscle layer in estrus were prone to distinguished than in diestrus. On the high frequency, endometrium and circular muscle layer were always distinguished regardless of estrous cycle. In vivo, uterine endometrium and circular muscle layer were observed regardless of estrus and ultrasonographic frequency. On the low frequency, stratum vasculare and longitudinal muscle layer were not likely to be distinguished in diestrus, but estrus. On the high frequency, stratum vasculare and longitudinal muscle layer were observed regardless of estrous cycle. Also, every uterine structure was easily distinguished on high frequency than low frequency owing to precision of distinction in layers. The difference of results followed by the experiments conditions between in vitro and in vivo was that uterine endometrium and circular muscle layer in diestrus in vitro were difficult to be distinguished and uterine lumen was observed during whole estrous cycle. In vivo, It was founded that the distinction of stratum vasculare and logitudinal muscle layer in diestrus was complicated and uterine lumen was observed during only estrus. In view of the result so far achieved, normal uterine structure divided in 5 layers on ultrasonography was accorded with microscopic organization, uterine structure was likely to be observed during estrus than diestrus, high frequency checkup than low frequency, and uterine endometrium, circular muscle, stratum vasculare was easily observed regardless of estrous cycle and ultrasonographic frequency.

• The Korean Journal of Pharmacology
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• v.5 no.2
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• pp.121-127
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• 1969

Antimuscarinic agent like antispasmodic actions of licorice alkaloidal fraction, obtained from the Glycyrrhiza glabra L., was compared with that of atropine quantitatively. For this purpose, the author calculated the kinetic constants and $ED_{50}$ for acetylcholine antagonism by these drugs on rat ileum and guinea-fig ileum longitudinal muscle according to Paton's theoretical equations describing the combination of an antagonist drug with its receptors. The results are as follows. 1. On rat ileum. a) Licorice alkaloidal fraction $K_1$ (association rate constant)=$4.078{\times}10^2\;(s^{-1}\;gm^{-1}\;ml)$ $K_2$ (dissociation rate constant)=$6.986{\times}10^{-4}\;(s^{-1})$ $ED_{50}(K_2/K_1)=1.772{\times}10^{-6}(gm/ml)$ b) Atropine $K_1=5.136{\times}10^6$, $K_2=7.714{\times}10^{-4}$, $ED_{50}=1.408{\times}10^{-10}$ 2. On guinea-pig ileum longitudinal muscle a) Licorice alkaloidal fraction $K_1=1.30{\times}10^2$, $K_2=1.25{\times}10^{-3}$ $ED_{50}=9.58{\times}10^{-6}$ b) Atropine $K_1=5.75{\times}10^6$, $K_2=1.54{\times}10^{-3}$ $ED_{50}=2.68{\times}10^{-10}$ Above results present that 1 r of licorice alkaloidal fraction has equal fotency of acetylcholine antagonism with $8.5{\times}10^{-5}r$ of atropine on rat ileum, $2.8{\times}10^{-5}r$ on guinea-pig ileum longitudinal muscle. This facts suggest that the site and numbers of licorice alkaloid receptors of guinea-pig ileum are different from that of rat ileum. Besides, it also gives a suggestion that licorice alkaloidal fraction may be a partial antagonist on guinea-pig ileum in this experimental conditions.

• Archives of Reconstructive Microsurgery
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• v.7 no.2
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• pp.88-94
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• 1998

Free vascularized fibular transplantation is one of the most useful living bone reconstructive procedure in microsurgical field. Concerning about donor site morbidity, the donor has minor problems of ankle stability and muscle power weakness and transient peroneal nerve symptoms. That problems can be minimized with longitudinal splitted osteotomy on the donor fibula if the bone defect in recipient site is not so large. Half splitted fibula with peroneal arterial pedicle which contains nutrient artery and periosteal vessels, grafted bone can survive with those vascular supplies. Authors underwent five cases of half splitted free vascularized fibular transplantation from 1985. There were no evidence of devascularization in all cases, we can minimized donor morbidity with leaving half fibula intact on donor site. The problem of that technique is technically demanding in longitudinal splitting of bone without damage to peroneal nutrient vessels and periosteal soft tissues which attached to the bone. Authors can propose longitudinal half fibular transplantation is one of modification in free vascularized bone transplantation that minimize donor defect.

• Physical Therapy Korea
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• v.4 no.1
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• pp.1-10
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• 1997

The purposes of this study were to compare the electromyographic activities from the rectus femoris, vastus medialis oblique portion, vastus medialis longitudinal portion, and vastus lateralis during straight leg raising (SLR) and quadriceps setting (QS) exercise and to determine which exercise is more effective to selectively strengthen the vastus medialis. Thirty two healthy subjects <19 men, 13 women) participated in the study. All participants performed SLR and QS exercises while electromyographic activity was recorded from EMG surface electrodes. Statistical analysis demonstrated significantly greater values for the vastus medialis oblique portion, vastus medialis longitudinal portion, and vastus lateralis activities during QS exercise than for those during SLR exercise. However, the rectus femoris muscle activity between SLR and QS exercises was not significantly different. The ratios of vastus lateralis to vastus medialis during SLR and QS exercise were not significantly different. These results show that QS exercise is more effective for strengthening the quadriceps muscle on the whole than the SLR exercise. As for selective methods of strengthening vastus medialis specifically, both SLR and QS exercises are useful.

• Korean Journal of Veterinary Research
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• v.25 no.1
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• pp.1-5
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• 1985

The morphological development of the small intestinal tissues of the Korean native goat were observed by light microscopy. Samples were taken from a 60-, 90-, 120-day-old fetus, a newborn goat and a 30-day-old goat. The results were summarized as follows; 1. In the small intestine of 60-day-old fetus, the apexes and sides of villi were covered with a simple columnar epithelium, and intervilous areas and mucosal ridges were still covered with stratified epithelium of two to six cell layers. Mesenchymal tissues formed lamina propria, circular muscle layer and serosa. The numbers of villi per cross section of the small intestine (NVPCS) were 10 to 18. 2. In 90-day-old fetus, intervillous areas and mucosal ridges of the organ were covered with simple columnar epithelium. Goblet cells in epithelium and outer longitudinal muscle layer often appeared. NVPCS were 35 to 60 and Brunner's glands were appeared. 3. In 120-day-old fetus, Brunner's glands of the duodenum and circular connection of outer longitudinal muscle layer were formed, NVPCS were 50 to 87. 4. In newborn goat, Peyer's patches were fully formed and NVPCS were 50 to 87. 5. In 30-day goat, the small intestine was fully matured and NVPCS were 81 to 102.

• Journal of the Korean Society of Physical Medicine
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• v.11 no.3
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• pp.59-63
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• 2016

PURPOSE: The purpose of the present study is to examine the effects of sole Pilates based breathing on the thicknesses of the abdominal muscles. METHODS: Twenty adults aged in their 20-30s were randomly selected. The selection criteria were those who had no medical history of pain due to musculoskeletal system, neurologic, or orthopedic diseases within six months before the selection. The thicknesses of the abdominal muscles (the transversus abdominis muscle, internal oblique abdominal muscle, and external oblique abdominal muscle) of the subjects were measured using ultrasonography before and after Pilates breathing. The study was approved by the research ethics committee of the Catholic University of Pusan. RESULTS: After Pilates based breathing, the thicknesses of the transversus abdominis muscle, the internal oblique abdominal muscle, and the external oblique abdominal muscle increased significantly. In particular, the thickness increase rate of the transversus abdominis muscle was shown to be the highest. CONCLUSION: Sole Pilates based breathing is expected to be effective for the improvement of trunk stability through strengthening of abdominal muscles, because it induces the coordinated contraction of the transversus abdominis muscle and other abdominal muscle. It will be need the Longitudinal study for identify to long term effects of Pilates based breathing.

• Physical Therapy Rehabilitation Science
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• v.5 no.2
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• pp.78-83
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• 2016

Objective: The purpose of this study was to investigate whether the intrinsic foot muscle training method can improve the medial longitudinal arch in patients with chronic ankle injury and with pronated feet, as well as to investigate for the most effective exercise method for these patients. Design: Randomized controlled trial. Methods: Thirty men and women with pronated feet had participated in this study and were allocated to either the short foot exercise group (SFEG) or the towel curl exercise group (TCEG) randomly. SFEG and TCEG underwent exercises three times a week for 8 weeks, with three sets per day, totalling up to 5 minutes per day. The navicular drop test (NDT) was used in order to assess for changes in the medial longitudinal arch and the Cumberland ankle instability tool (CAIT) was used to assess for ankle instability of the chronic ankle sprain patients. Results: There was a significant increase in CAIT scores in the SFEG (p<0.05) and a significant difference between groups was presented (p<0.05). The NDT scores were significantly decreased in both groups (p<0.05). In the SFEG, the NDT scores were more decreased than in the TCEG (p<0.05). Conclusions: These results suggest that short foot exercises are more effective in providing intrinsic foot muscle training for patients with pronated feet among chronic ankle sprain patients. Furthermore, short foot exercises may be used to provide ankle stability.

• Journal of the Korean Society of Physical Medicine
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• v.7 no.4
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• pp.471-480
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• 2012

PURPOSE: The object of the present study is to investigate the effects of the insole supporting medial longitudinal arch while walking in spastic cerebral palsy with pes planus. METHODS: Ten spastic bilateral cerebral palsy children with pes planus participated in this study. The insole were custom-made for the individual child. Muscle activity was measured by surface EMGs attached on tibialis anterior (TA), gastrocnemius (GA), vastus medialis oblique (VMO), biceps femoris long head (BF). temporal-spatial parameters such as velocity, step length, stride length, stance time, toe angle were collected while the subjects walked on the GAITRite system. RESULTS: The results of the present study were summarized as follows: 1. Muscle activities in mean EMGs while walking: Left VMO, Right TA, Left BF and GA revealed significant reductions after applying insole. 2. Muscle activities in peak EMGs while walking: Left TA and BF demonstrated reductions significantly after applying insole. 3. There were improvements in temporal-spatial gait parameters with insole: velocity, both step length, Right stride length and Right toe angle were increased(p<.05). CONCLUSION: Therefore the current study demonstrated that insole supporting the medial longitudinal arch would be effective on gait of the spastic cerebral palsy with pes planus.