The purpose of this study was to evaluate the clinical and microbiological outcomes following the use of 30% minocycline-loaded polycaprolacton film and 2% minocycline-loaded gel that was applied locally into pockets combined with scaling and root planing. 25 human subjects who were non-pregnant, non-lactating, aged 20-50 and diagnosed as moderate to advanced adult periodontitis were enrolled. Subjects were excluded if they had a history of severe acute or chronic systemic disease, if they required antibiotic prophylaxis for dental treatment for any reason, or if they reported a history suggestive of hypersensitivity reactions to minocycline or tetracycline. 4quadrants that had several teeth with a 5-8mm probing pocket depth and radiographic evidence of alveolar bone loss for each patient were selected and divided into test sites and control sites according to the split-mouth design. Scaling and root planing was done for each site at baseline(0week). Test sites received the minocycline gel and strip and control sites had saline irrigation. The patients received both treatments simyltaneously. Subgingival irrigation of sterile saline was applied to the control sites for approximately 30 seconds. Minocycline strip and gel was applied into the periodontal pocket at 1, 2, 3, 4 weeks each after scaling and root planing in the test sites. The clinical and microbiological measurements were made at baseline and at the follow-up visits 6, 10, 14, 20 weeks. The results of this study were as follows; 1. The sulcular bleeding index, probing pocket depth and Periocheck test was significantly reduced and the relative proportions of spirochetes and motile rods were significantly reduced and the proportion of cocci was correspondingly increased, in locally delivered minocycline strip group compared to saline irrigation group. 2. In locally delivered minocycline gel group, The effect was the same with minocycline strip group as compared with saline irrigation therapy. 3. There was no significant differences between minocycline strip group and minocycline gelgroup. In conclusion, minocycline HCl local drug delivery combined with scaling and root planing may provide added improvement of clinical and microbiological responses by inhibiting bacterial recolonization of treated sites. It is suggested that the local administration of minocycline-HCl in the periodontal pocket is effective when combined with subgingival mechanical debridement.
An, Phil-Gyun;Eom, Seong-Jun;Kim, Yong-Gyun;Cho, Han-Sol;Kim, Sang-Bum
Journal of Korean Society of Rural Planning
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v.27
no.4
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pp.55-70
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2021
In this study, in the field of remote sensing, where the scope of application is rapidly expanding to fields such as land monitoring, disaster prediction, facility safety inspection, and maintenance of cultural properties, monitoring of rural space and surrounding environment using UAV is utilized. It was carried out to verify the possibility, and the following main results were derived. First, the aerial image taken with an unmanned aerial vehicle had a much higher image size and spatial resolution than the aerial image provided by the National Geographic Information Service. It was suitable for analysis due to its high accuracy. Second, the more the number of photographed photos and the more complex the terrain features, the more the point cloud included in the aerial image taken with the UAV was extracted. As the amount of point cloud increases, accurate 3D mapping is possible, For accurate 3D mapping, it is judged that a point cloud acquisition method for difficult-to-photograph parts in the air is required. Third, 3D mapping technology using point cloud is effective for monitoring rural space and rural resources because it enables observation and comparison of parts that cannot be read from general aerial images. Fourth, the digital elevation model(DEM) produced with aerial image taken with an UAV can visually express the altitude and shape of the topography of the study site, so it can be used as data to predict the effects of topographical changes due to changes in rural space. Therefore, it is possible to utilize various results using the data included in the aerial image taken by the UAV. In this study, the superiority of images acquired by UAV was verified by comparison with existing images, and the effect of 3D mapping on rural space monitoring was visually analyzed. If various types of spatial data such as GIS analysis and topographic map production are collected and utilized using data that can be acquired by unmanned aerial vehicles, it is expected to be used as basic data for rural planning to maintain and preserve the rural environment.
This study was performed to estimate the effects of cultured bone cell inoculated on porous type hydroxyaptite for the regeneration of the artificial alveolar bone defect. In this experiment 3 beagle dogs were used, and each of them were divided into right and left mandible. Every surgical intervention were performed under the general anesthesia by using with intravenous injection of Pentobarbital sodium(30mg/Kg). To reduce the gingival bleeding during surgery, operative site was injected with Lidocaine hydrochloride(l:80,000 Epinephrine) as local anesthesia. After surgery experimental animal were feeded with soft dietl Mighty dog, Frisies Co., U.S.A.) for 1 weeks to avoid irritaion to soft tissue by food. 2 months before surgery both side of mandibular 1st premolar were extracted and bone chips from mandibular body were obtained from all animals. Bone cells were cultured from bone chips obtained from mandible with Dulbecco's Modified Essential Medium contained with 10% Fetal Bovine Serum under the conventional conditions. Porous type hydroxyapatite were immerse into the high concentrated cell suspension solution, and put 4 hours for attachin the cells on the surface of hydroxyapatite. Graft material were inserted on the artificial bone defect after 3 days of culture. Before insertion of cellinoculated graft material, scanning electronic microscopic observation were performed to confirm the attachment and spreading of cell on the hydroxyapatite surface. 3 artificial bone defects were made with bone trephine drill on the both side of mandible of the experimental animal. First defect was designed without insertion of graft material as negative control, second was filled with porous replamineform hydroxyapatite inoculated with cultured bone marrow cells as expermiental site, and third was filled with graft materials only as positive control. The size of every artificial bone defect was 3mm in diameter and 3mm in depth. After the every surgical intervention of animals, oral hygiene program were performed with 1.0% chlorhexidine digluconate. All of the animals were sacrificed at 2, 4, 6 weeks after surgery. For obtaining histological section, tissus were fixed in 10% Buffered formalin and decalcified with Planko - Rycho Solution for 72hr. Tissue embeding was performed in paraffin and cut parallel to the surface of mandibular body. Section in 8um thickness of tissue was done and stained with Hematoxylin - Eosin. All the specimens were observed under the light microscopy. The following results were obtained : 1. In the case of control site which has no graft material, less inflammatory cell infiltration and rapid new bone forming tendency were revealed compared with experimental groups. But bone surface were observed depression pattern on defect area because of soft tissue invasion into the artificial bone defect during the experimental period. 2. In the porous hydroxyapatite only group, inflammatory cell infiltration was prominet and dense connective tissue were encapsulated around grafted materials. osteoblastic activity in the early stage after surgery was low to compared with grafted with bone cells. 3. In the case of porous hydroxyapatite inoculated with bone cell, less inflammatory cell infiltration and rapid new bone formation activity was revealed than hydroxyapatite only group. Active new bone formation were observed in the early stage of control group. 4. The origin of new bone forming was revealed not from the center of defected area but from the surface of preexisting bony wall on every specimen. 5. In this experiment, osteoclastic cell was not found around grafted materials, and fibrovascular invasion into regions with no noticeable foreign body reaction. Conclusively, the cultured bone cell inoculated onto the porous hydroxyapatite may have an important role of regeneration of artificial bone defects of alveolar bone.
Kim, Jin-Hee;Youn, Mi-Ra;Bang, So-Young;Sim, Ji-Yeon;Kang, Hee-Rae;Yang, Hyun-Won
Development and Reproduction
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v.14
no.4
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pp.287-295
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2010
It was recently reported that nesfatin-1/NUCB2, which is secreted from the brain, controls appetite and energy metabolism. The purpose of this research was to confirm whether or not the protein and its binding site should have been expressed in the mouse reproductive organs and to know the possible effects of nesfatin-1 on the reproductive function. Using the ICR female mouse ovary and uterus, the expression of NUCB2 mRNA was confirmed with the conventional PCR and the relative amount of NUCB2 mRNA in the tissues was analyzed with real-time PCR. Immunohistochemical staining was performed using the nesfatin-1 antibody to investigate the nesfatin-1 protein expression and the biotin conjugated nesfatin-1 to confirm the binding site for nesfatin-1 in the ovary. Furthermore, in order to examine if the expression of NUCB2 mRNA in the ovary and uterus is affected by gonadotropin, its mRNA expression was analyzed after PMSG administration into mice. As a result, the expression level of NUCB2 mRNA in the ovary and the uterus was as much as the expression level in hypothalamus. As a result of the immunohistochemical staining, nesfatin-1 proteins were localized at the theca cells, the interstitial cells, and some of the luteal cells. However, the granulosa cells in the follicles did not stain. Interestingly, the oocytes in the some follicles were stained with nesfatin-1. On the other hand, nesfatin-1 protein binding sites were displayed at the theca cells and the interstitial cells near the tunica albuginea. After PMSG administration the expression level of NUCB2 mRNA was increased in the ovary and the uterus. These results demonstrate that for the first time the nesfatin-1 and its binding site were expressed in the ovary and NUCB2 mRNA expression was controlled by gonadotropin, suggesting an important role in the reproductive organs as a local regulator. Therefore, further study is needed to elucidate the functions of nesfatin-1 on the reproductive organs.
Korean Journal of Agricultural and Forest Meteorology
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v.3
no.4
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pp.185-198
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2001
This study was conducted to reveal the effects of local climatic conditions on reproductive growth in a mature stand of Korean white pine based on climatic estimates. For this, the reproductive growth such as production and characteristics of cone and seed were first measured and summarized for seven years from 1974 to 1980. The local climatic conditions in the study site were also estimated by both a topoclimatological method and a spatial statistical technique. The local climatic conditions were then correlated with and regressed on the growth factors to reveal the relationships between the climatic estimates and the reproductive growth. Average number of conelet formation per tree showed highly negative correlation with some climatic variables related to minimum temperature in the year of flower bud differentiation. Especially, the most significant negative correlation were found between average of the minimum temperature for June and July of flower bud differentiation year and the number of conelet formation. There was no significant correlation between the number of cone production and climatic variables. However, total precipitation from December of the flowering year to February of the cone production year showed the most high correlation (r=0.6036) with the number of cone production. It was found that significant climatic variables affecting the amount of cone drop and cone drop percentage were the sum of cloudy days from June of the flowering year to August of the cone production year. Positive correlation was significantly recognized between the average weight of empty seed per cone and total precipitation from December of the flowering year to February of the cone production year. For the percentage of empty seed, five climatic variables among 19 variables were significantly correlated at 10% level. The average weight of a cone showed negative correlation with total precipitation from June of the flowering year to August of the cone production year. It was also found that average weight of a seed had highly negative correlation with total precipitation from December of the flowering year to February of the cone production year. The average weight of cone coat was negatively correlated with two climatic variables derived from clear days, which are sum of clear days from November of the flowering year to March of the cone production year and sum of clear days from December of the flowering year to February of the cone production year. On the other hand, it showed positive correlation with mean temperature of May in the flowering year. The exactly same results were obtained in correlation analysis for the percentage of cone coat.
Korean Journal of Agricultural and Forest Meteorology
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v.16
no.4
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pp.403-417
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2014
Increased frequency of climate extremes is another face of climate change confronted by humans, resulting in catastrophic losses in agriculture. While climate extremes take place on many scales, impacts are experienced locally and mitigation tools are a function of local conditions. To address this, agrometeorological early warning systems must be place and location based, incorporating the climate, crop and land attributes at the appropriate scale. Existing services often lack site-specific information on adverse weather and countermeasures relevant to farming activities. Warnings on chronic long term effects of adverse weather or combined effects of two or more weather elements are seldom provided, either. This lecture discusses a field-specific early warning system implemented on a catchment scale agrometeorological service, by which volunteer farmers are provided with face-to-face disaster warnings along with relevant countermeasures. The products are based on core techniques such as scaling down of weather information to a field level and the crop specific risk assessment. Likelihood of a disaster is evaluated by the relative position of current risk on the standardized normal distribution from climatological normal year prepared for 840 catchments in South Korea. A validation study has begun with a 4-year plan for implementing an operational service in Seomjin River Basin, which accommodates over 60,000 farms and orchards. Diverse experiences obtained through this study will certainly be useful in planning and developing the nation-wide disaster early warning system for agricultural sector.
Application of the soil nailing method is continuously extended in maintaining stable excavations and slopes. Occasionally, however, ground anchor support system may not be used because of space limitations in urban excavation sites nearby the existing structures. In this case, soil nailing system with relatively short length of nails could be efficiently adopted as an alternative method. The general soil nailing support system, however, may result in excessive deformations particularly in an excavation zone of the existing weak subsoils. Pretensioning the soil nails then, could play important roles in reducing deformations mainly in an upper part of the nailed-soil excavation system as well as improving local stability. In the present study, the analytical procedure and design technique are proposed to evaluate maximum pretension force and stability of the pretensioned soil nailing system. Also proposed are techniques to determine the required thickness of a shotcrete facing and to estimate probability of a failure against the punching shear. The predicted results are compared with the limited measurements obtained from the excavation site constructed by using the pretensioned soil nails. Based on the proposed procedure and technique, effects of the radius of a influence circle and dilatancy angle on the thickness of a shotcrete facing, bonded length and safety factors are analyzed. In addition, effects of the reduction of deformations expected by pretensioning of the soil nails are examined in detail throughout an illustrative example and FLAC$^{2D}$ program analysis.s.
Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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2014.10a
/
pp.25-48
/
2014
Increased frequency of climate extremes is another face of climate change confronted by humans, resulting in catastrophic losses in agriculture. While climate extremes take place on many scales, impacts are experienced locally and mitigation tools are a function of local conditions. To address this, agrometeorological early warning systems must be place and location based, incorporating the climate, crop and land attributes at the appropriate scale. Existing services often lack site-specific information on adverse weather and countermeasures relevant to farming activities. Warnings on chronic long term effects of adverse weather or combined effects of two or more weather elements are seldom provided, either. This lecture discusses a field-specific early warning system implemented on a catchment scale agrometeorological service, by which volunteer farmers are provided with face-to-face disaster warnings along with relevant countermeasures. The products are based on core techniques such as scaling down of weather information to a field level and the crop specific risk assessment. Likelihood of a disaster is evaluated by the relative position of current risk on the standardized normal distribution from climatological normal year prepared for 840 catchments in South Korea. A validation study has begun with a 4-year plan for implementing an operational service in Seomjin River Basin, which accommodates over 60,000 farms and orchards. Diverse experiences obtained through this study will certainly be useful in planning and developing the nation-wide disaster early warning system for agricultural sector.
Objective : Since the outbreak of the coronavirus disease 2019 (COVID-19) pandemic, neurointerventionists have been increasingly concerned regarding the prevention of infection and time delay in performing emergency thrombectomy procedures in patients with acute stroke. This study aimed to analyze the effects of changes in mechanical thrombectomy protocol before and after the COVID-19 pandemic on procedure time and patient outcomes and to identify factors that significantly impact procedure time. Methods : The last-normal-to-door, first-abnormal-to-door, door-to-imaging, door-to-puncture, and puncture-to-recanalization times of 88 patients (45 treated with conventional pre-COVID-19 protocol and 43 with COVID-19 protection protocol) were retrospectively analyzed. The recanalization time, success rate of mechanical thrombectomy, and modified Rankin score of patients at discharge were assessed. A multivariate analysis was conducted to identify variables that significantly influenced the time delay in the door-to-puncture time and total procedure time. Results : The door-to-imaging time significantly increased under the COVID-19 protection protocol (p=0.0257) compared to that with the conventional pre-COVID-19 protocol. This increase was even more pronounced in patients who were suspected to be COVID-19-positive than in those who were negative. The door-to-puncture time showed no statistical difference between the conventional and COVID-19 protocol groups (p=0.5042). However, in the multivariate analysis, the last-normal-to-door time and door-to-imaging time were shown to affect the door-to-puncture time (p=0.0068 and 0.0097). The total procedure time was affected by the occlusion site, last-normal-to-door time, door-to-imaging time, and type of anesthesia (p=0.0001, 0.0231, 0.0103, and 0.0207, respectively). Conclusion : The COVID-19 protection protocol significantly impacted the door-to-imaging time. Shortening the door-to-imaging time and performing the procedure under local anesthesia, if possible, may be required to reduce the door-to-puncture and door-to-recanalization times. The effect of various aspects of the protection protocol on emergency thrombectomy should be further studied.
Korean Journal of Agricultural and Forest Meteorology
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v.10
no.3
/
pp.82-93
/
2008
In order to ensure a standardized data analysis of the eddy covariance measurements, Hong and Kim's quality control program has been updated and used to process eddy covariance data measured at two levels on the main flux tower at Gwangneung site from January to May in 2005. The updated program was allowed to remove outliers automatically for $CO_2$ and latent heat fluxes. The flag system consists of four quality groups(G, D, B and M). During the study period, the missing data were about 25% of the total records. About 60% of the good quality data were obtained after the quality control. The number of record in G group was larger at 40m than at 20m. It is due that the level of 20m was within the roughness sublayer where the presence of the canopy influences directly on the character of the turbulence. About 60% of the bad data were due to low wind speed. Energy balance closure at this site was about 40% during the study period. Large imbalance is attributed partly to the combined effects of the neglected heat storage terms, inaccuracy of ground heat flux and advection due to local wind system near the surface. The analysis of wind direction indicates that the frequent occurrence of positive momentum flux was closely associated with mountain valley wind system at this site. The negative $CO_2$ flux at night was examined in terms of averaging time. The results show that when averaging time is larger than 10min, the magnitude of calculated $CO_2$ fluxes increases rapidly, suggesting that the 30min $CO_2$ flux is influenced severely by the mesoscale motion or nonstationarity. A proper choice of averaging time needs to be considered to get accurate turbulent fluxes during nighttime.
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