• 제목/요약/키워드: loach

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미꾸라지(Misgurnus mizolepis) Apolipoprotein A-I cDNA의 구조, 분자계통 및 발현 특징 분석 (Characterization of Mud Loach (Misgurnus mizolepis) Apolipoprotein A-I: cDNA Cloning, Molecular Phylogeny and Expression Analysis)

  • 이윤호;노재구;김근용;조영선;남윤권;김동수
    • 한국양식학회지
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    • 제20권1호
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    • pp.65-72
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    • 2007
  • 우리나라 주요 담수어종인 미꾸라지(Misgurnus mizolepis)로 부터 apolipoprotein A-I (apoA-I) cDNA를 분리하고 그 구조, 분자 계통 및 발현 특징을 분석하였다. 미꾸라지 apoA-I cDNA는 254개의 아미노산을 암호화하고 있는 762 bp의 ORF를 포함하고 있었으며 아울러 24 bp의 5'UTR 및 293 bp의 3'UTR(종결 코돈 및 poly A tail 제외)를 갖고 있었다. 미꾸라지 apoA-I은 여타 척추동물 apoA-I과의 다중배열 시 염기서열에서는 많은 차이를 나타내었지만 단백질의 구조적 특징은 높은 상동성을 보였고, 또한 척추동물의 apoA-I들과의 분자계통을 분석한 결과, 종래 알려진 분류학적 위치와 비교적 잘 일치하였다. 미꾸라지 apoA-I mRNA는 RT-PCR 분석을 통해 간 및 뇌 조직에서 분석한 다른 조직보다 유의적으로 높게 발현하는 것으로 나타났고, 특히 간에서 가장 높은 발현을 보였다. 수정시부터 부화 후 14일까지 초기 발생 및 치어에서의 apoA-I mRNA 발현을 조사한 결과 수정 8시간째부터 급격한 발현의 증가가 시작되어 이후 지속적으로 높은 발현 수준을 유지하였다.

벼 재배 논 서식 미꾸리에 대한 농약의 영향 (Toxicological effects of pesticides on loach in rice paddy)

  • 박연기;박경훈;주진복;경기성;김병석;신진섭;류갑희;배철한;이규승
    • 농약과학회지
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    • 제7권2호
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    • pp.131-138
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    • 2003
  • 수도용 농약의 미꾸리에 대한 영향 평가를 위한 야외포장 시험방법을 설정하기 위하여 미꾸리가 서식하고 있는 실제 포장에서 수도용 농약인 아이비 엽제 (17%), 다수진 엽제 (3%), 그리고 부타크로르 입제 (5%)의 미꾸리에 대한 영향을 시험하였다. 시험 케이지는 미꾸리의 스트레스를 최소화 할 수 있는 아래 윗면이 트인 사각 스테인레스 케이지가 적당하였고, 수온 유지와 해적으로부터 보호하기 위한 차광망과 방조 망의 사용이 필수적이었다. 아이비 입제, 다이아지논 입제 그리고 부타크로르 입제에 대한 7일간 미꾸리 누적 치사율은 각각 10, 55, 22% 이었다. 특히 약제 처리 2일후 논물 중 평균 농약잔류량은 각각 1.67, 0.22, 0.26 mg/L이었으며, 치사율은 각각 7, 50, 17%로 나타났다. 이는 실내 급성독성시험을 통한 각각의 급성독성 시험결과와 유사하였다.

생물자원의 논 잡초 생물학적 방제 효과와 그에 따른 수서동물과 곤충 종수 및 벼 수량 변화 (Effects of Biocontrol Agents on Weed Control, Abundance of Aquatic Animals and Insects, and Yield in Paddy Rice Fields)

  • 최효정;서상영;최선우;이창규;엄미정;김주희;안민실;최우정
    • 한국환경농학회지
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    • 제42권1호
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    • pp.63-70
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    • 2023
  • Golden apple snails (Pomacea canaliculat) (GAS) are widely used for weed control in rice cultivation. However, concerns on the ecological risk of invasive GAS species are increasing. This study aimed to evaluate the overall impacts of GAS on weed control, abundance of aquatic animals and insects, and rice yield, in comparison with that from other biocontrol agents such as loach (Misgurnus mizolepis) and catfish (Silurus asotus), which are alternatives for biological weed control in rice paddy cultivation. Field experiments included five treatments; control, herbicide, GAS, loach, and catfish. During the rice growth, weed appearance and biological abundance were monitored, and at harvest, the rice yield was determined. Weed control efficiency was the highest for GAS treatment (100%), followed by that for herbicide (95.8%), loach (57.5%), and catfish treatments (31.7%). Insect abundance was considerably decreased in GAS treatment due to heavy weed removal, which affects the habitat of aquatic animals and insects. The amount of rice yield (unit: kg 10 a-1) was in the order of GAS (798.9) > loach (708.1) = herbicide (700.7) > catfish (629.4) > control (496.0). Therefore, considering the weed control efficiency, biological abudance, and rice yield, loach could be a potential alternative for biological weed control in organic rice farming. However, the activity of the biocontrol agents are susceptible to environmental conditions; therefore, further studies under different conditions are essential to confirm these findings.

Morphology and Histochemistry of the Skin of the Mud Loach, Misgurnus mizolepis, in Relation to Cutaeneous Respiration

  • Park, Jong-Young;Kim, Ik-Soo;Kim, So-Young
    • Animal cells and systems
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    • 제5권4호
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    • pp.303-308
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    • 2001
  • The skin structure of Misgurnus mizolepis was studied based on the microanatomical investigation of skin fragments taken from four regions. The epidermis was distinguished by two types of skin glands, a small mucous cell and a large club cell. The mucous cell was acid sulfomucins (some sialomucins) but the club cell did not give any histochemical tests for mucosubstances. The presence of a well defined lymphatic system with small lymphocytes was established in the stratum germinativum layer of the epidermis. A large number of blood capillaries run very close to each other just below the basement membrane, and a definite area giving AB and PAS positive was present between the basement membrane and scale. These structural features of skin in M. mizolepis seem to be closely related with cutaneous respiration.

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The Structure of Visual Cells in the Retina of the Pond Loach, Misgurnus anguillicaudatus (Pisces; Cobitidae)

  • Kim, Chi-Hong;Kim, Jae-Goo;Park, Jong-Young
    • Applied Microscopy
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    • 제45권4호
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    • pp.254-258
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    • 2015
  • A histological study on the retina of Misgurnus anguillicaudatus was carried out by light microscopy and scanning electron microscopy as part of getting information about relation between its habitat and visual cells. The visual cells of the retina in M. anguillicaudatus, a bottom-dwelling freshwater pond loach in stagnant or slow waters such as swamps, reservoirs and paddy fields, consists of double cones and large rods. The cones form a row mosaic pattern in which the partners of double cones are linearly oriented with a large rod. In a double cone, the two members are unequal such that one cone may be longer than the other.

미꾸라지 렉틴 성분의 생화학적 특성 (Biochemical Studies on Lectins from Misgurnus spp.)

  • 정시련;김장환;소명숙;김무경;현태금;전경희
    • 약학회지
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    • 제35권5호
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    • pp.444-455
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    • 1991
  • Two kinds of new lectin fractions (LOA-I, LOA-II) were obtained from loach (Misgurnus spp.) meat by 0.15 M NaCl extraction, salt fractionation, ion exchange and hydroxyapatite column chromatographies. On polyacrylamide gel electrophoresis, LOA-I exhibited one major and a few minor bands, but LOA-II exhibited three minor bands. The partially purified loach lectins agglutinated not only erythrocytes of human B and AB type, rabbit, dog, but also murine splenic lymphocytes. Agglutinability was relatively labile at various pH and stable at increasing temperature, but was not affected by tested several metal ions. By the sugar specificity test, D-glucosamine and metyl-$\beta$-galactopyranose inhibited agglutinating activity at a final concentration of 3 mM. The lectins contained relatively high amounts of aspartic acid, valine and leucine, but sulfur containing amino acids, cystein, methionine and isoleucine were not determined. LOA-I, LOA-II lectins were nonmitogenic toward murine lymphocytes.

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한국자생종 종개 Orthrias toni(Pisces: Balitoridae)의 생활사 (Life History of the Siberian Stone Loach Orthrias toni (Pisces: Balitoridae) in the Buk Stream, Goseong, Korea)

  • 고명훈
    • 한국어류학회지
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    • 제29권3호
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    • pp.197-204
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    • 2017
  • 강원도 고성군 북천에서 종개 Orthrias toni의 생활사를 조사하여 생물학적 특징을 밝혔다. 성장은 수온이 $10^{\circ}C$ 이상 되는 4월부터 10월까지 빨랐으나 수온이 $10^{\circ}C$ 이하인 11월부터 이듬해 3월까지는 거의 멈추었다. 연령은 전장빈도분포도로 볼 때 산란기인 5월을 기준으로 전장 60~81 mm는 만 1년생, 82~99 mm는 만 2년생, 100~119 mm는 만 3년생, 120~140 mm는 만 4년생 이상으로 추정되었다. 암컷과 수컷의 연령별 전장 범위는 거의 동일하였고, 성비(♂/♀)는 0.73으로 암컷이 많았다. 산란기는 생식소성숙도(GSI)로 추정한 결과 5월이었으며, 이때의 수온은 $14{\sim}17^{\circ}C$였다. 생식소는 모든 연령이 성숙하였고, 연령이 높아지면서 포란수는 급격히 증가하였으며, 평균 $4,460{\pm}3,302$개였다. 성숙란의 크기는 연령과 상관없이 직경 $0.87{\pm}0.03mm$로 비슷하였다.

전남 여수지역 산업체에 근무하는 근로자의 식품 및 음식의 기호도 조사 (Food and Meal Preference of Workers in the Chonnam Yeosu Industrial Area)

  • 한현미;최일수;정복미
    • 대한지역사회영양학회지
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    • 제14권4호
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    • pp.392-405
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    • 2009
  • This study was conducted to investigate food and meal preference of workers (435 male and 212 female) in the Chonnam Yeosu industrial area. The results of the survey were analyzed by principal components analysis. The results were obtained as follows: 19.3% of the subjects were twenties, 28.3% were thirties, 28.8% were forties and 23.7% were fifties. Females liked rice rolled in dried laver, rice cakes, janchi-noodle and breads, on the other hand males liked thick beef soup, loach soup, an eel stew and soju. The young liked instant noodles, fried chicken, sweet and sour pork, pork cutlet, pizza, hamburger, ham, sausage and fruit beverage, on the other hand the old liked a fish pot stew, loach soup, eel stew, fish boiled in soy with spices, panfried fish, sea slug, ascidian, bunder, green laver and boiled burdock-lotus root in soy. Females who are young liked hamburger and sweet and sour pork whereas males who are young liked instant noodles, pork cutlet, ham, sausage and fruit beverage. Aged females liked fish boiled in soy with spices, panfried fish, bunder, green laver, boiled burdock-lotus root in soy whereas aged males liked a fish pot stew, loach soup, eel stew, sea mussel and oysters. Boiled rice was located within the middle irrespective of age and sex, but noodles, wheat flour meal, fast foods and fruits were situated at the young female side. Aged males liked soup and pot stew. Young males liked meats and eggs whereas fish and shellfish and kimch were located at the aged people side. Aged females liked sea weeds and most people disliked vegetables but females liked some vegetables irrespective of age. Processed foods, salted foods, and alcohol were generally disliked foods by subjects but males liked those foods. Soybean curd was liked more males than females, and teas, except coffee, was liked by males. (Korean J Community Nutrition 14(4) : 392${\sim}$405, 2009)

Altered expression of mud loach (Misgurnus mizolepis; Cypriniformes) hepcidin mRNA during experimental challenge with non-pathogenic or pathogenic bacterial species

  • Lee, Sang-Yoon;Kim, Dong-Soo;Nam, Yoon-Kwon
    • 한국어병학회지
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    • 제24권3호
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    • pp.279-287
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    • 2011
  • Transcriptional response patterns of mud loach (Misgurnus mizolepis; Cypriniformes) hepcidin, a potential ortholog to human hamp1, in response to experimental challenges with non-pathogenic and pathogenic bacterial species were analyzed based on the semi-quantitative reverse transcription-PCR assay. Mud loach hepcidin transcripts were much more preferentially induced by pathogenic bacterial species (Edwardsiella tarda and Vibrio anguillarum) causing apparent pathological symptoms than by non-pathogenic species (Escherichia coli and Bacillus thuringiensis) displaying neither clinical signs nor mortality. However in overall, the induced amounts of hepcidin transcripts were positively related with the number of bacterial cells delivered in both pathogenic and non-pathogenic bacterial species. Inducibility of hepcidin transcripts were variable among three tissues examined (liver, kidney and spleen) in which kidney and spleen were more responsive to the bacterial challenge than liver. Time course expression patterns of hepcidin mRNAs after challenge were different between groups challenged with pathogenic and non-pathogenic species, although the overall pattern of hepcidin expression was in accordance with that generally observed in battery genes appeared during early phase of inflammation. Fish challenged with E. coli (non-pathogenic) showed the significant induction of hepcidin transcripts within 24 hr post injection (hpi) but the level was rapidly declined to the basal level either at 48 or 96 hpi. On the other hand, hepcidin transcript levels in E. tarda (pathogenic)-challenged fish were continuously elevated until 48 hpi, then downregulated at 96 hpi, although the level at 96 hpi was still significantly higher than control level observed in non-challenged fish. This expression pattern was consistent in all the three tissues examined. Taken together, our data indicate that hepcidin is tightly in relation with pathological and/or inflammation status during bacterial challenge, consequently providing useful basis to extend knowledge on the host defensive roles of hepcidin under infectious conditions in bony fish.