• Title/Summary/Keyword: llex macropoda

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Acyl-CoA: Cholesterol Acyltransferase Inhibitors from llex macropoda

  • Im Kyung-Ran;Jeong Tae-Sook;Kwon Byoung-Mog;Baek Nam-In;Kim Sung-Hoon;Kim Dae-Keun
    • Archives of Pharmacal Research
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    • v.29 no.3
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    • pp.191-194
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    • 2006
  • Twigs from llex macropoda were extracted with MeOH, and the concentrated extracts were partitioned with $CH_2Cl_2$, EtOAc, n-BuOH, and $H_2O$. Repeated column chromatography of the $CH_2Cl_2$ fraction ultimately resulted in the isolation of two compounds, via activity-guided fractionation, using ACAT inhibitory activity measurements. According to the physico-chemical data, the chemical structures of these isolated compounds were identified as lupeol (1) and betulin (2). Compounds 1 and 2 were shown to inhibit the activity of hACAT-1 and hACAT-2 in a dose-dependent manner, and compounds 1 and 2 inhibited hACAT-1 with $IC_{50}$ values of 48 and $83{\mu}M$, respectively.