• Title/Summary/Keyword: live blood analysis

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Extracting Blood Vessels through Similarity Analysis and Intensity Correction (유사도 분석과 명암 보정을 통한 혈관 추출)

  • Jang Seok-Woo
    • Journal of Internet Computing and Services
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    • v.7 no.4
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    • pp.33-43
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    • 2006
  • This paper proposes a method to extract coronary arteries effectively in the angiography, In general. DSA(Digital Subtraction Angiography) is a well-established technique for the visualization of coronary arteries, DSA involves the subtraction of a mask image, an image of a heart before the injection of contrast medium, from a live image, However, this technique is sensitive to the movement of background and can cause wrong detection due to the variance of background intensity between two images. Therefore, this paper solves the structural problem resulted from background movement by selecting an image which has the least difference of movement through the similarity analysis of background texture, and it extracts only the blood vessels effectively through local intensity correction of the selected images, Experimental results show that the proposed method has the lower false-detection rate and higher accuracy rate than existing methods.

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Exposure Assessment of volatile Organic Matters (VOCs) Using Exposure Biomarker in the Residents Living Near Petrochemical Industry Areas (공단지역 주민에서 휘발성 유기오염물질의 호흡기 노출에 대한 생체지표 평가)

  • 신동천;박성은;임영욱;양지연;김명수
    • Environmental Analysis Health and Toxicology
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    • v.15 no.3
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    • pp.81-91
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    • 2000
  • Residents who live near petrochemical industrial areas are exposed to a variety of petrochemicals, including benzene or benzene-containing liquids. It is a serious concern because some VOCs are carcinogens naturally present in petroleum and gasoline. The aim of this study was to assess the exposure to VOCs, measured by personal/indoor/outdoor air sampling, and to estimate the relationship between the air samples and biological monitoring data. Through biological monitoring, we investigated VOCs in blood and s-phenylmercapturic acid (s-PMA) , minor urinary metabolites of benzene. The external benzene exposure of subjects was measured using passive dosimeters and urinary s-PMA and blood-benzene were determined by GC/MS. More than 80% of subjects were detected for m-xylene, ethylbenzene, and toluene in blood samples and not detected at all for chloroform, 1 , 1 , 1 -trichloroethylene, and tetrachloroethylene. The mean concentration of benzene in the breathing zone of residents was 6.3 $\mu\textrm{g}$/m$^3$, personal, indoor and outdoor concentrations were strongly correlated to each other. s-PMA detected in all subject samples was affected by personal exposure (p< 0.05) and the level was different by age (p< 0.01). Blood benzene was not affected by external benzene during these periods .

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Application of ELISA for the Detection of Sulfamethazine Residue in Live Cattle

  • Lee, H.J.;Lee, M.H.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.3
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    • pp.378-381
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    • 2001
  • Sulfamethazine has been widely used in swine for prevention or treatment of infections. Recently, the safety of the drug to consumers has been questioned because of carcinogenic effects. To prevent unwanted drug residues entering the human food chain, both government authorities and industries have established extensive control measures. The demands for reliable, simple, sensitive, rapid and low-cost methods for residue analysis of foods are increasing nowadays. In this study, we established a rapid prediction test for the detection of cattle with violative tissue residues of sulfamethazine. The recommended therapeutic dose of sulfamethazine (withdrawal time, 15 days) was administered to each of 10 cattle. Blood was sampled before drug administration and during the withdrawal period. The concentration of sulfamethazine in plasma, determined by a semi-quantitative ELISA, was compared to that of an internal standard (10 ppb). The absorbance ratio of internal standard to sample (B/Bs) was employed as an index to determine whether drug residues in cattle tissues were negative or positive. That is, a B/Bs ratio less than 1 was considered residue positive and if larger than 1 was considered negative. All 10 plasma samples from non-treated cattle showed negative to sulfamethazine. Sulfamethazine was detected in plasmas of treated cattle until Day 7 of withdrawal period. The present study showed that the semi-quantitative ELISA could be easily adapted in predicting residues of sulfamethazine in live cattle.

A study on the health status of physical therapy college mans (물리치료과 학생의 건강 상태에 대한 연구)

  • YOUN Hee-Jong;LEE In-Hak
    • The Journal of Korean Physical Therapy
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    • v.3 no.1
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    • pp.59-73
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    • 1991
  • The purpose of this study was to phychosomatic health status of physical therapy college mans. 130college mans analysis sex, habitation, religion, blood type, drinking, somoking by the todai health index questionaire. THI was modified front cornell medical index. Physical therapy college man showed complain score (E) Nervousness. (J) Mental irritability. (H) Impulsiveness. (F) Aggressiveness. (G) Irregular life (I), (L) Multiple subjective symtom(I), Live scale(L), (K) Depression. (C) Digestive, (A), (B) Respiratory(A), Eye and Skin(B). (D) Mouth and Anus.

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The Radiobiological Evaluation on Abnormal Delivery of Cattle around Nuclear Power Plant using Micronucleus Assay in Lymphocyte (림프구 미소핵 측정법을 이용한 원자력발전소 주변 소의 이상산에 대한 방사선 생물학적 평가)

  • 김세라;김성호
    • Journal of Veterinary Clinics
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    • v.20 no.3
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    • pp.364-368
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    • 2003
  • Cytogenetic and hematological analysis was performed in peripheral blood from the cattle associated with abnormal delivery around nuclear power plant area. The frequency of micronuclei (MN) in peripheral blood lymphocytes from cattle was used as a biomarker of radiobiological effects resulting from exposure to environmental radiation. An estimated dose of radiation was calculated by best fitting linear-quadratic model based on the radiation-induced MN data over the range from 0 Gy to 4 Gy from the bovine lymphocytes with in vitro irradiation. MN rates in live malformed calf, dams of malformed calves and other cattle living in the same barn from the regions around nuclear power plant, and cattle in control area were 9/1000, 10.8/1000, 13.3/1000 and 10.0/1000, respectively. There were no significant differences in MN frequencies and hematological values between the cattle associated with abnormal delivery around nuclear power plant area and those of control area. This study indicates that the congenital abnormalities near nuclear power plant seemed to be caused by other aetiology.

Application of ELISA for the Detection of Penicillin Antibiotic Residues in Live Animal

  • Lee, H.J.;Lee, M.H.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.11
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    • pp.1604-1608
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    • 2000
  • Penicillin antibiotics such as penicillin G, ampicillin and amoxicillin have been widely used in the pig industry to control salmonellosis, bacterial pneumonia, and urinary tract infections. Extensive use of antibiotics in veterinary clinics has resulted in tissue residues and bacterial resistance. To prevent unwanted drug residues entering the human food chain, extensive control measures have been established by both government authorities and industries. The demands for reliable, simple, sensitive, rapid and low-cost methods for residue analysis of foods are increasing. In this study, we established a rapid prediction test for the detection of pigs with unacceptable tissue residues of penicillins. The recommended therapeutic doses of three penicillins, penillin G (withdrawal time, 7 days), ampicillin (withdrawal time, 7 days) and amoxicillin (withdrawal time, 14 days), were administered to three groups of 20 pigs each. Blood was sampled before drug administration and during the withdrawal period. The concentration of penicillins in plasma, determined by a semi-quantitative ELISA, were compared to that of internal standard, 4 ppb, which corresponded to the Maximum Residue Limit in milk. The absorbance ratio of internal standard to sample (B/Bs) was employed as an index to determine whether drug residues in pig tissues were negative or positive. That is, a B/Bs ratio less than 1 was considered residue positive, and larger than 1 negative. All 60 plasma samples from pigs were negative to three penicillins at pretreatment. Penicillin G could be detected in the plasma of the treated pigs until day 4 post-treatment and ampicillin until day 2, whereas amoxicillin could be detected until day 10 of its withdrawal period. The present study showed that the semi-quantitative ELISA could be easily adapted to detect residues of penicillin antibiotics (penicillin G, ampicillin and amoxicillin) in live pigs.

Application of ELISA for the Detection of Oxytetracycline Residue in Live Animals

  • Lee, H.J.;Lee, M.H.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.12
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    • pp.1775-1778
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    • 2000
  • Oxytetracycline has been widely used in the cattle industry to control pneumonia, shipping fever, foot rot, bacterial enteritis, and uterine infections. Extensive use of antibiotics in veterinary clinics has resulted in residues in tissue and bacterial resistance to antibiotics. To prevent unwanted drug residues from entering the human food chain, extensive control measures have been established by both government authorities and industries. The demands for reliable, simple, sensitive, rapid and low-cost methods for residue analysis of foods are increasing. In this study, we established a rapid test for tissue residues of oxytetracycline in cattle. The recommended therapeutic dose of oxytetracycline (withdrawal time, 14 days) was administered to 10 cattle. Blood samples were collected from each cow before drug administration and during the withdrawal period. The concentration of oxytetracycline in plasma, determined by a semi-quantitative ELISA, was compared to that of the internal standard, 10 ppb. The absorbance ratio of internal standard to sample (B/Bs) was employed as an index to determine whether the residues in cattle tissues were negative or positive. That is, a B/Bs ratio less than 1 was considered as residue positive and that greater than 1 as negative. Based on this criterion, all plasma samples from cattle were negative to oxytetracycline at pre-treatment. Oxytetracycline could be detected in the plasma treated cattle until day 14 post-treatment. The present study showed that the semi-quantitative ELISA could be easily adapted in predicting tissue residues for oxytetracycline in live cattle.

A Study on the Effects of Smoking Habit to Health Status in Some Male Employees (일부 남성 직장인에서 관찰된 흡연행태가 건강상태에 미치는 성향연구)

  • 한영미;이시백
    • Korean Journal of Health Education and Promotion
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    • v.7 no.1
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    • pp.54-63
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    • 1990
  • This study aims to find out the prevalence of smoking, and to analyze the effect of smoking for health status, and then to emphasize the necessity of stop smoking. The data used in this study are obtained from periodic health care programe at Health Care Center in a suburban hospital, and selected 435 males who have occupation. The independent varibles chosen for the analysis are general charactersitic variables and smoking habit. The dependent variables are designed to cover the health status of individual cases, and include blood pressure, blood cholesterol level with HDL-cholesterol and blood triglyceride level, recent symptoms and recently being managed diseases. The result of this study are summarized as follows. 1) Percentage of smoker by the age groups is highest in 4th decade, being 71.1%. The second and third ranks are 6th and 7th decades, being 53.5% and 44.4%, respectively. 2) In the view of socio-economic levels, smoking rate is higher in the groups who live at rural area and whoes occupation is labor or merchant. Smoking rate is significantly higher in the heavy drinking group. 3) Among the atherosclerotic risk factors, which include hypertension, HDL-cholesterol by total cholesterol ratio lower than 0.2 and triglyceride level higher than 200gm/dl, hypertension was not statistically associated with smoking, but others revealed statistically high association with smoking. 4) The groups who have the symptoms of severe fatigue, gastrointestinal symptoms, pulmonary symptoms, palpitation and chest tightness havepositive association with smoking. 5) The groups who have hypertension with cardiovascular diseases and gastrointestinal diseases showed highly significant association with non-smoking. 6) In relation of the smoking habit to the atherosclerotic risk factor index, smokers have more atherosclerotic risk factors, but that is not statistically significant. 7) In relation of the smoking habit to the recent symptom index, smokers have more symptoms than non-smokers with statistical significance. In conclusion, smokers have worse health status than non-smokers especially in the atherosclerotic risk factors such as hypertension or abnormal blood lipid status and have more symptoms such as severe fatigue, gastrointestinal symptoms, pulmonary symptoms, palpitation and chest tightness. And the campaingn against smoking should direct for the male in 4th decade because they have highest smoking rate.

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The Change of Plasma Neurotransmitters Concentration after Aromatherapy in Dementia Patients (라벤더향 흡입 후 치매환자의 혈장 중 신경전달물질의 농도변화)

  • Lee, Kyung-Bok;Kang, Young-Sook
    • YAKHAK HOEJI
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    • v.56 no.2
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    • pp.86-91
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    • 2012
  • The objective of this study is to examine how different concentration of neurotransmitters in plasma between patients with dementia and normal people regarding the inhalation of lavender oil. This study subjects were 9 elderly patients with dementia who live in nursing home and 9 normal women. Before and after inhalation, they were collected blood sample. Norepinephrine (NE), serotonin (5-HT), dopamine (DA), and r-aminobutyric acid (GABA) concentration analysis were performed. Before inhalation, dementia patients were significantly different with the normal group in GABA and DA, NE. Following inhalation in experimental group, dementia patients and normal group were only significantly increased in 5-HT. But it did not significantly change in the other neurotransmitters. After inhalation, dementia patients were significantly different with the normal group in GABA and 5-HT. This result suggests that the increase of 5-HT release by the inhalation of lavender oil related to reduce the behavioral and psychological symptoms of dementia.

Analysis of Case Reports for Korean medical treatment of Irritable Bowel Syndrome (과민성 장증후군의 한방치료 증례논문 분석)

  • Lee, Yeon-weol;Yoo, Hwa-seung;Cho, Chong-kwan
    • Journal of Haehwa Medicine
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    • v.25 no.1
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    • pp.87-97
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    • 2016
  • Objectives : The purpose of this study was to present the clinical guidelines for Korean medical treatment of Irritable Bowel Syndrome. Methods : Reports for Korean medical treatment of Irritable Bowel Syndrome were collected, analyzed and summarized from the Electronic journal search since 2000. Results : In Korean medicine treatment of irritable bowel syndrome used herbs, acupuncture, moxibustion, herbal acupuncture, and auricular acupuncture. Herb medicines were administered according to the patient's case by a prescription of considering physical condition and symptoms. Acupuncture and moxibustion therapies stimulate the median points of LR(足厥陰肝經), ST(足陽明胃經), LI(手陽明大腸經), SI(手太陽小腸經). The assessment items for Korean medical treatment of Irritable Bowel Syndrome were abdominal symptoms, defecation, autonomic nervous system changes, stress, Live blood condition and mental health. Conclusions : Through continued research need to develop better treatment strategies for Korean treatment of Irritable Bowel Syndrome.