• Korean Journal Plant Pathology
• /
• v.10 no.3
• /
• pp.211-214
• /
• 1994

Paulowina witches'-broom mycoplasma-like organism (PWB-MLO) was transmitted experimentally to periwinkle (Catharanthus roseus L.) plant by tobacco leaf bug (Cyrtopeltis tenuis Reuter). Adults of the leaf bugs were allowed to feed on the witch's-broom infected paulownia (Paulownia tomentoas Steud.) trees for three weeks to insure the acquisition of PWB-MLO and then transferred to healthy seedlings of periwinkle and paulownia plants. In 25∼35 days after transfer of the viruliferous leaf bugs, six out of the ten periwinkle plants showed‘little-leaf’symptoms, while the paulowina seedlings remained symptomless. Presence of MLO in the infected periwinkle tissue was diagnosed by fluorescence microscopy and MLO particles were observed under electron microscope, confirming the transmission of PWB-MLO to periwinkle.

• Journal of Forest and Environmental Science
• /
• v.39 no.1
• /
• pp.49-54
• /
• 2023

For unknown reasons, a few trees in a private chestnut orchard in Icheon si, Gyunggi-do suffered leaf chlorosis and growth decline. Based on symptoms, phytoplasma was a probable cause. Leaf samples were collected from two symptomatic and non-symptomatic trees in the orchard for phytoplasma detection. An amplicon of about 1.2 bp size was obtained from both symptomatic trees by PCR with the universal 16S rDNA primers. Sequences of these amplicons were found to have 99% nucleotide sequence identity to the corresponding genomic region of 16SrIII (X-disease group). More than 100 phytoplasma isolates, such as Candidatus phytoplasma pruni, Milkweed yellows phytoplasma, Goldenrod yellows phytoplasma, Tsuwabuki witches'-broom phytoplasma, Candidatus Phytoplasma trifolii, etc. were involved in the list. Phylogenetic analysis revealed that the sequence obtained in this study closely clustered with Candidatus phytoplasma groups. While one of the amplicons shared 91% identity with the Candidatus phytoplasma castaneae, the other shared only 47%. It needs further analysis and investigation to determine the exact taxonomy. Meanwhile, based on the analysis of the sequences, chlorosis, and small leaves were associated with phytoplasma.

• Korean journal of food and cookery science
• /
• v.11 no.3
• /
• pp.233-236
• /
• 1995

This study was carried out to investigate the possibility of improving texture and flavor of Dasik made of pine pollen with waxy rice flour and Angelica gigas leaf powder addition. Pine pollen Dasik was not affected in shape, flavor, texture, taste, melting degree and unpleasant flavor by add of 10％ rice flour. Flavor, texture and melting degree were little bit affected by add of 30％ rice flour but all items got worse with same amount of rice flour addition. Add of Angeleica leaf powder by 10％ improved a little the texture while flavor was decreased.

• Current Research on Agriculture and Life Sciences
• /
• v.21
• /
• pp.31-37
• /
• 2003

The study aimed to identify leaf sheath through the investigation a characteristic of leaf by planting depth and leaf sheath function through the investigation of effect of leaf sheath removals on growth and flowering in forcing of Lilium cultivars. The number of leaf sheath of 'Jolanda', 'Dream Land', 'Casablanca' were 6, 5, 11 in 0cm planting depth and 6, 5, 14 in 6cm planting depth. The number of leaf sheath in 'Jolanda' and 'Dream Land' were little different in according to planting depth. The other hand, the number of leaf sheath in 'Casablanca' were increased 3 when it were planted in 6cm planting depth. As leaf sheath were removed, plant height and stem root growth were decreased in 'Le Reve' and 'Casablanca'. Whereas the growth of basal root and flower number varied among of Lillium and stem diameter, the days of flowering and flower size were little different.

• Current Research on Agriculture and Life Sciences
• /
• v.21
• /
• pp.17-21
• /
• 2003

This study aimed to identify leaf sheath function through the investigation of effect of removal times and removed number of leaf sheath on growth and flowering in gladiolus. The leaf sheath influenced on the growth and flowering regardless of light condition at early growth stages. When total leaf sheath were removed in early growth stages, flower stalk length and spike length remarkably decreased. Whereas days of flowering were little different by leaf sheath removal. Also when removed numbers of leaf sheath were 1, 2 and 3, the growth and flowering were little different compared to control. But when removed numbers of leaf sheath were 4, flower stalk length decreased above 25cm compared to others treatment, spike length and number of flower decreased and stem diameter became thick abnormally.

• Journal of the Korean Society of Tobacco Science
• /
• v.18 no.1
• /
• pp.66-75
• /
• 1996

This study was carried out to investigate the effects of high temperature and humidity on the ageing of processed leaf tobacco. Four(1993) or six(1994) grades (Both flued-cured and burley) of processed leaf packed in carton box were stored under the natural and artificial conditions. When the Processed leaf was stored in the controlled mom at 40℃ with 75% R.H.(1993) for 40 days, the leaf pH was decreased. The decreasing rate of leaf pH was similar to that of leaf stored under the natural warehouse condition for 15 months. The degree of lightness and yellowness of leaf also decreased, while the decaying or darkening of the leaf was observed. When the processed leaf was stored in the controlled room at 35℃ with 65% R.H.(1994) for 90 days, the pH of flue-cured was decreased 0.22, which was similar to that of the leaf stored under the natural warehouse condition for 15 months, and the lightness, and redness of the leaf were higher than those of the control. As compared with the leaf stored under the natural condition for 2 years, the smoking quality of leaf stored under this condition was similar or somewhat better. The pH of burley tobacco changed little compared to that of flue-cured during storage in this study.

• Journal of the Korean Society of Tobacco Science
• /
• v.23 no.1
• /
• pp.31-39
• /
• 2001

This study was carried out to investigate the changes of physical and chemical properties and the usability of long-term stored leaf tobacco. The physical chemical properties of the flue-cured and burley leaves produced in 1993, processed in 1994 were analysed from Nov. 1996 to Nov. 1999. The pH and moisture content in leaf decreased slowly until 4 years’storage after processing, while those of leaf changed little thereafter. However, total sugar content continuously decreased until 5 years after processing. The filling capacity increased and shatter resistance index decreased in long-term stored leaf. The sensory test, cilia stasis and the chemical components of cigarette smoke had no significant differences between short and long-term stored leaves. When the processed leaves were stored till 5 years after processing, there were no deteriorative effects on quality and usability of leaf tobacco. Therefore, it is considered that the processed leaf may be stored for 5 years or more under the inevitable situation.

• Journal of Ecology and Environment
• /
• v.32 no.1
• /
• pp.61-65
• /
• 2009

We compared two nondestructive methods for leaf area estimation using leaves of 16 common plant species classified into six types depending on leaf shape. Relatively good linear relationships between actual leaf area (LA) and leaf length (L), width (W), or the product of length and width (LW) were found for ordinary leaves with lanceolate, oblanceolate, linear and sagitttate shapes with entire margins, serrate margins, mixed margins with a entire form and shallow lobes, and ordinary incised margins. LA was better correlated with LW than L or W, with $R^2$ > 0.91. However, for deeply incised lobes, LA estimation using LW showed low correlation coefficient values, indicating low accuracy. On the other hand, a method using photographic paper showed a good correlation between estimates of area based on the mass of a cut-out leaf image on a photographic sheet (PW) and actual leaf area for all types of leaf shape. Thus, the PW method for LA estimation can be applied to all shapes of leaf with high accuracy. The PW method takes a little more time and has a higher cost than leaf estimation methods using LW based on leaf dimensions. These results indicate that researchers should choose their nondestructive LA estimation method according to their research goals.

• Journal of the Korean Society of Tobacco Science
• /
• v.3 no.1
• /
• pp.58-65
• /
• 1981

This study was carried out to investigate the influence of distance of planting holes (51, 45, and 40 cm) and number of plants per a hole(4, 5, 6 plants) on agronomic characteristics, yield, and quality of an aromatic tobacco, Sohyang. The results are as follows: 1. Relative light intensity increased by widening the distance of holes. 2. Leaf area per a plant or per a leaf, and leaf length and width increased by widening the distance of holes and decreased by increasing the number of plants per a hole. But L. A. 1. increased by increasing the number of plants per a hole. Leaf shape index (Leaf length/Leaf width) showed little differences among treatments. 3. Dry weight of leaf, root, and stem per a plant decreased by increasing the number of plants per a hole. 4. Total nitrogen decreased by increasing number of plants per a hole and in the case of narrow distance of holes, but nicotine, reducing sugar, ether-extract and ash showed little differences. 5. Yield per 10a decreased by widening the distance of holes. 6. Quality(price per kg) was improved by increasing the number of plants per a hole at the Plot of 51m distance of holes. But there was no variation at the Plot of 45cm. And quality was decreased at the plot of 40cm distance of holes by increasing the number of Plants per a hole. 7. Price per 10a was highest in the plot of which plant spacing was $90\times$40cm and the number of plants per a hole was 4 (11112 plant/10a).

• Journal of Korean Society of Forest Science
• /
• v.98 no.5
• /
• pp.576-583
• /
• 2009

Based on the stem analysis and biomass measurement of 36 trees and 1,576 branches in Pinus sylvestris var. mongolica (Mongolian pine) plantations of Northeast China, this study was conducted to develop estimation model equation for leaf biomass of a single tree and branch, to examine the vertical distribution of leaf biomass in the crown, and to evaluate the proportional ratios of biomass by tree parts, stem, branch, and leaf. The results indicated that DBH and crown length were quite appropriate to estimate leaf biomass. The biomass of single branch was highly correlated with branch collar diameter and relative height of branch in the crown, but not much with stand density, site quality, and tree height. Weibull distribution function would have been appropriate to express vertical distribution of leaf biomass. The shape parameters from 29 sample trees out of 36 were less than 3.6, indicating that vertical distribution of leaf biomass in the crown was displayed by bell-shaped curve, a little inclined toward positive side. Apparent correlationship was obtained between leaf biomass and branch biomass having resulted in linear function equation. The stem biomass occupied around 80% and branch and leaf made up about 20% of total biomass in a single tree. As the level of tree class was increased from class I to class V, the proportion of the stem biomass to total biomass was gradually increased, but that of branch and leaf became decreased.