• The Journal of the Korean dental association
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• v.19 no.7 s.146
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• pp.609-614
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• 1981

Effects of steroid hormones on the collagen biosynthesis in aorta and uterus were studied with ovariectomized Sprague-Dawley rats. Effects of administration of hormones, such as estrogen, testosterone and prednisolone, to the ovariectomized animals were studied, comparing with the control. Each group was injected with ³H-proline and sacrificed, followed by removals of aorta and uterus. Separations and quantitative analyses of proline and hydroxyproline were performed by means of thin layer chromatography; and radioactivities of the separated amino acids were assayed by liquid scintillation counter. Normally the incorporation of ³H-proline into hydroxyproline was greater in uterus than in aorta, and collagen turnover rate of uterus was observed rapid as well than that of aorta. In the two tissues from ovariectomized rats, the incorporation rate of ³H-proline into hydroxypoline was markedly decreased than that of the former. Changes in the turnover rate of collagen in these tissues were not observed. Decrease in ³H-proline incorporation into collagen in ovariectomized rats was markedly antagonized by estrogen, but not influenced by prednisolone in the tissues tested.

• The Korean Journal of Zoology
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• v.37 no.2
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• pp.222-231
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• 1994

밤바구미 유충기의 whole body로부터 cyclic AMP(CAMP)를 추출하여 농도 변화를 측정하였고 cyclic AMP-dependent protein kinase(PKA)를 부분 정제하여 활성도 변화를 조사하여 CAMP 농도와 PKA 활성도와의 소장관계를 비교하였다 CAMP 농도와 PKA 활성도는 HPLC와 liquid scintillation counter를 이용하여 측정하였다 CAMP 농도는 밤바구미 유충에서 월동전에 0.57 UMlg로 가장 높았고, 월동중에 0. 14 UMIS로 감소하였다가 월동후에 0.29 UMlg로 증가하였다 또한 PKA 활성도는 월동전에 2.56unit/mg로 가장 높았으며, 월동중에 0.62 unit/mg로 감소하다가 월동후에 07 unit/mg로 다시 증가하여 CAMP 농도 변화와 유사한 경향을 나타내었다. 이는 월동전에 휴면에 대비하여 최대의 취식으로 지방체 축적이 가장 많았고, 월동중에는 지방체의 소비가 증대되.기 때문에 감소하였다가, 월동후 휴면 종결과 유충-번데기 탈피를 준비하기 위해 'CAMP 농도와 PKA 활성도는 다시 증가하였다.

• The Journal of the Korean dental association
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• v.19 no.6 s.145
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• pp.529-533
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• 1981

The effect of insulin on the collagen metabolism in dental pulp and gingiva was studied using Sprague-Dawley rat and ³H-proline was injected intraperitoneally and then sacrificed according to predetermined schedule. Lower incisor pulp and gingiva were removed immediatedly and hydrolysed with acid. After digestion, proline and hydroxyproline were separated and analysed quantitatively employing thin-layer chromatography and radioactivity of each amino acid was counted by liquid scintillation counter. The label incorporated into lower incisor pulp and gingiva and radioactivity of ³H-hydroxyproline were increased in insulin administered group. And disappearance rate of ³H-hydroxyproline was decreased. Those results indicated that amino acid, I.e. proline transport and collagen biosynthesis were increased in dental pulp and gingiva by insulin administration.

• Bulletin of the Korean Chemical Society
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• v.4 no.3
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• pp.128-132
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• 1983

The self-diffusion experiment of water was performed across two series of tactic poly(2-hydroxyethyl methacrylate), P(HEMA) membranes prepared by crosslinking with various amount of hexamethylene diisocyanate (HMDIC). The tagging material was tritium hydroxide (THO) and the efflux of THO was counted on a Liquid Scintillation Counter. The transport data of THO show that the permeability decreases as the amount of HMDIC increased from 2.5 to 10 mole % and the self-diffusions coefficient shows a parallel trend with it. The diffusivity data was discussed in terms of the change of water structural orderliness within membranes. Using the relation between viscosities and diffusivities derived from Eyring's absolute rate theory, the corresponding viscosities of water within two series of tactic P(HEMA) membranes were obtained. From this, it is seen that the viscosity of water within tactic P(HEMA) membranes may have the same values with those of supercooling water whose temperature ranges from -28 to -$36^{\circ}C.$.

• Nuclear Engineering and Technology
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• v.55 no.6
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• pp.2107-2111
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• 2023

The aim of the study is the activity concentration measurements of organic and inorganic ¹⁴C in the discharges of JSC "Institute of Nuclear Materials" (INM). In INM the research water-water reactor "IVV-2M" is operating. Collecting of ¹⁴C species was performed using a ¹⁴C sampler with a chromium oxide and platinum catalysts at different temperatures: 400, 550 and 700 ℃. The measurements of ¹⁴C activity were performed using a liquid scintillation counter. The share of organic ¹⁴C in emissions ranged from 0.30 to 0.84 and depends on the temperature of the catalyst, core structure and reactor operating mode.

• Journal of Radiation Protection and Research
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• v.41 no.4
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• pp.395-401
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• 2016

Background: Liquid scintillation counters (LSCs) are commonly used as an analytical method for detecting $^{222}Rn$ in groundwater because they involve a simple sample pretreatment and allow high throughout with an autosampler. The Quantulus 1220 is the best-selling LSC in Korea, but its production was stopped. Recently, a new type of LSC, the 300SL, was introduced. In this study, the 300SL was compared with the Quantulus 1220 in order to evaluate the ability of each apparatus to detect $^{222}Rn$ in groundwater. Materials and Methods: The Quantulus 1220 and 300SL were used to detect the presence of $^{222}Rn$. Radon gas was extracted from a groundwater sample using a water-immiscible cocktail in a LSC vial. The optimal analytical conditions for each LSC were determined using a $^{222}Rn$ calibration source prepared with a $^{226}Ra$ source. Results and Discussion: The optimal pulse shape analysis level for alpha and beta separation was 80 for the Quantulus 1220, and the corresponding pulse length index was 12 in the 300SL. The counting efficiency of the Quantulus 1220 for alpha emissions was similar to that of the 300SL, but the background count rate of the Quantulus 1220 was 10 times lower than that of the 300SL. The minimum detectable activity of the Quantulus 1220 was $0.08Bq{\cdot}L^{-1}$, while that of the 300SL was $0.20Bq{\cdot}L^{-1}$. The analytical results regarding $^{222}Rn$ in groundwater were less than 10% different between these LSCs. Conclusion: The 300SL is an LSC that is comparable to the Quantulus 1220 for detecting $^{222}Rn$ in groundwater. Both LSCs can be applied to determine the levels of $^{222}Rn$ in groundwater under the management of the Ministry of Environment.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.7 no.1
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• pp.1-18
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• 1979

인삼(人蔘)사포닌으로 처리(處理)한 모발(毛髮)의 인장강도(引張强度)와 신장도(伸張度)를 collagen 가수분해물(加水分解物)로 처리한 모발(毛髮)과 비교(比較)하였다. 모발(毛髮)에 인삼(人蔘)사포닌 흡수량(吸收量)은 high speed liquid chromatography로 측정(測定)하였으며 liquid scintillation counter로 확인(確認)하였다. 모발(毛髮)에 collagen 가수분해물(加水分解物)의 흡수량(吸收量)은 모발(毛髮)을 collagen 가수분해물(加水分解物) 용액(溶液)속에 침적(沈積)시켰을 때 처음 30분(分) 동안은 많은 량(量)의 흡착(吸着)이 일어났으나 1시간(時間) 경과후(經過後)부터는 더 이상(以上)의 증가(增加)는 보이지 않았다. 이와는 반대(反對)로 인삼(人蔘)사포닌의 경우(境遇)는 침적시간(沈積時間)의 증가(增加)에 비례(比例)하여 흡수량(吸收量)의 증가(增加)를 나타내었다. 실험결과(實驗結果)는 인삼(人蔘)사포닌이 collagen 가수분해물(加水分解物) 보다 모발(毛髮)에 더욱 효과적(效果的)임을 보여 주었고, 그리고 인삼(人蔘)사포닌의 모발(毛髮)에 대(對)한 흡수(吸收)와 인장강도(引張强度)의 증가(增加)는 모발(毛髮)의 strain-stress곡선(曲線)과 인삼(人蔘)사포닌의 계면활성(界面活性)에 의(依)해 설명(說明)하였다.

• Analytical Science and Technology
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• v.28 no.5
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• pp.353-360
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• 2015

Boiling is an efficient removal method to reduce radon in groundwater when ventilating indoor air. 13 groundwater samples with various radon concentrations were used to evaluate the reduction rate of radon with heating temperature and time. The groundwater samples were obtained by Bladder pump and on-situ measurements such as dissolved oxygen (DO) and hydrogen concentration (pH) and so on were carried out by a flow cell system isolated from the ambient atmosphere environment. All samples for measuring radon in groundwater were analyzed by liquid scintillation counter (LSC). The experiment result showed that increasing groundwater temperature enhanced radon removal rate but the initial radon concentration with high level lowered the removal rate. This means that radon reduction in groundwater by heating needs more heating energy and longer heating time with radon concentrations. Radon removal rate in groundwater, therefore, mainly depends on the initial radon concentration, heating temperature, and heating time.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.4 no.4
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• pp.329-334
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• 2006

The depth distributions of total $^3H$ and $^{14}C$ activities were characterized for the activated shielding concrete from a decommissioning of KRR-2 using the commercially available tube furnace and a liquid scintillation counter. The correlation of measurement results between $^3H,\;^{14}C$ and gammer emitter was evaluated to apply for estimating radionuclide inventory of the concrete waste generated from decommissioning KRR-2. The detection limits for $^3H$ and $^{14}C$ are 0.048 and 0.028 Bq/g respectively. The specific activities of the $^3H$ and $^{14}C$ tend to decrease exponentially as the depth of the concrete becomes deeper from the surface. In addition, the $^3H$ and $^{14}C$ activities were in good correlation with the $^{60}CO$ activities analysed for the shielding concrete of KRR-2.

• Journal of Radiation Protection and Research
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• v.33 no.1
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• pp.21-26
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• 2008

When the calibration on Liquid Scintillation Counter using the Solid $^3H$ Standard Source of 200,000DPM is executed, the uncertainty due to activity and geometry difference, exists. Therefore, this paper intends to evaluate environmental samples comparatively accurately as decreasing this uncertainty existing in the process of calibration. For this, measurements on samples manufactured by $^3H$ Standard Source and sensitivity study were performed. Also, this paper verified calibration results using Radioactivity-Error-Analysis Method, and evaluated quantitatively the effect by geometry and activity difference based on verification result. According to the result of sensitivity study, in case of using the exposure time of 75 sec and Repeat method, the measuring accuracy and precision of about $1{\sim}3%$ were increased in comparison with the existing method. By analysis result, the effect by activity difference did not appear, and a plastic cell existing into Teflon vial made a role as reflector. The less the effect of plastic cells are decreased, the more activity is high, and the effect of those can be neglected at the activity of 200,000 DPM.