• Proceedings of the PSK Conference
• /
• 2003.04a
• /
• pp.284.2-284.2
• /
• 2003

Using a column-switching technique. highly sensitive and selective semi-micro high-performance liquid chromatographic (HPLC) method has been developed for the determination of baclofen in human plasma. Following precipitation of plasma sample containing baclofen with zinc sulfate-acetonitrile, samples were directly injected on to the system. (omitted)

• Korean Chemical Engineering Research
• /
• v.49 no.5
• /
• pp.582-587
• /
• 2011

Holdup characteristics of bubble, wake and continuous liquid phases were investigated in bubble columns with viscous liquid media. Effects of column diameter(0.051, 0.076, 0.102 and 0.152 m ID), gas velocity($U_G$=0.02~0.16 m/s) and liquid viscosity(${\mu}_L$=0.001~0.050 $Pa{\cdot}s$) of continuous liquid media on the holdups of bubble, wake and continuous liquid phases were discussed. The three phase such as bubble, wake and continuous liquid phases were classified successfully by adapting the dual electrical resistivity probe method. Compressed filtered air and water or aqueous solutions of CMC(Carboxy Methyl Cellulose) were used as a gas and a liquid phase, respectively. To detect the wake as well as bubble phases in the bubble column continuously, a data acquisition system(DT 2805 Lab Card) with personal computer was used. The analog signals obtained from the probe circuit were processed to produce the digital data, from which the wake phase was detected behind the multi-bubbles as well as single bubbles rising in the bubble columns. The holdup of bubble and wake phases decreased but that of continuous liquid media increased, with an increase in the column diameter or liquid viscosity. However, the holdup of bubble and wake phases increased but that of continuous media decreased with an increase in the gas velocity. The holdup ratio of wake to wake to bubble phase decreased with an increase in the column diameter or gas velocity, however, increased with an increase in the viscosity of con-tinuous liquid media. The holdups of bubble, wake and continuous liquid media could be correlated in terms of operating variables within this experimental conditions as: ${\varepsilon}_B=0.043D^{-0.18}U_G^{0.56}{\mu}_L^{-0.13}$, ${\varepsilon}_W=0.003D^{-0.85}U_G^{0.46}{\mu}_L^{-0.10}$, ${\varepsilon}_C=1.179D^{0.09}U_G^{-0.13}{\mu}_L^{0.04}$.

• Journal of the Korean Society of Visualization
• /
• v.16 no.1
• /
• pp.48-53
• /
• 2018

In this study, the effect of injection pressure on the column diameter and droplet velocity of liquid jet with the weakly turbulent Rayleigh-like breakup mode is experimentally studied using digital microscopic holography (DMH). The injection nozzle has the diameter of $50{\mu}m$ and injection pressure is varied from 0.1 to 0.4 MPa. When the micro liquid jet is injected into still air, the double-pulsed holograms was recorded on a CCD sensor and numerically reconstructed in order to obtain well focused images. In this study, the liquid column diameter from $50{\mu}m$ orifice nozzle is shown to be changed slightly but the droplet velocity is increased proportionally as the injection pressure is increased.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.18 no.5
• /
• pp.402-409
• /
• 2006

For the design of a liquid direct contact cooling system, thermal and hydraulic analysis has been carried out. Well-known Zukauskas correlations are used to estimate the Nusselt number between the liquid refrigerant columns and the inlet airflow. The inlet air velocity is set at a typical value used in an actual showcase. For a constant column number, the best nozzle arrangement is determined for the maximum heat transfer. Heat transfer increases as the transverse pitch of the refrigerant column decreases. Among all the cases dealt with in the present study, the staggered arrangement with 140-columns of $14{\times}10$ shows the best thermal peformance and the expected temperature drop is $27.8^{\circ}C$. The effect of downstream refrigerant columns on the overall thermal performance is investigated as well.

• Korean Chemical Engineering Research
• /
• v.50 no.3
• /
• pp.499-504
• /
• 2012

Characteristics of overall heat transfer were investigated in a three-phase slurry bubble column with relatively low surface tension media, which has been frequently encountered in the fields of industry. The heat transfer phenomena was examined in the system which was composed of a coaxial vertical heater and a proper of bubble column. The heat transfer coefficient was estimated from the measured mean value of temperature difference between the heater surface and the column proper at the steady state condition. Effects of gas velocity ($U_G$), solid fraction in the slurry phase ($C_S$) and surface tension (${\sigma}_L$) of continuous liquid media on the overall heat transfer coefficient (h) in the bubble column were determined. The mean value of temperature difference was estimated from the data of temperature difference fluctuations with a variation of time. The amplitude and mean value of temperature difference fluctuations with respect to the elasped time appeared to decrease with decreasing the surface tension of liquid phase. The overall heat transfer coefficient between the immersed heated and the bubble column increased with an increase in the gas velocity or solid fraction in the slurry phase, but it decreased with an increase in the surface tension of continuous liquid media. The overall heat coefficient in the slurry bubble column with relatively low surface tension media was well correlated in term of operating variables and dimensionless groups within this experimental conditions.

• FOCUS: LIFE SCIENCE
• /
• no.1
• /
• pp.1.1-1.4
• /
• 2024

This article provides comprehensive guidance on the maintenance, cleaning, regeneration, and storage of silica-based HPLC (High-Performance Liquid Chromatography) columns. The general considerations emphasize the importance of using in-line filters and guard cartridges to protect columns from blockage and irreversible sample adsorption. While these measures help, contamination by strongly adsorbed sample components can still occur over time, leading to an increase in back pressure, loss of efficiency, and other issues. To maximize column lifetime, especially with UHPLC (Ultra-High Performance Liquid Chromatography) columns, it is advisable to use ultra-pure solvents, freshly prepared aqueous mobile phases, and to filter all samples, standards, and mobile phases. Additionally, an in-line filter system and sample clean-up on dirty samples are recommended. However, in cases of irreversible compound adsorption or column voiding, regeneration may not be possible. The document also provides specific recommendations for column cleaning procedures, including the flushing procedures for various types of columns such as reversed phase, unbonded silica, bonded normal phase, anion exchange, cation exchange, and size exclusion columns for proteins. The flushing procedures involve using specific solvents in a series to clean and regenerate the columns. It is emphasized that the flow rate during flushing should not exceed the specified limit for the particular column, and the last solvent used should be compatible with the mobile phase. Furthermore, the article outlines the storage conditions for silica based HPLC columns, highlighting the impact of storage conditions on the column's lifetime. It is recommended to flush all buffers, salts, and ion-pairing reagents from the column before storage. The storage solvent should ideally match the one used in the initial column test chromatogram provided by the manufacturer, and column end plugs should be fitted to prevent solvent evaporation and drying out of the packing bed.

• Proceedings of the Korean Society of Propulsion Engineers Conference
• /
• v.y2005m4
• /
• pp.155-158
• /
• 2005

The spray characteristics of liquid jet minted in subsonic cross-flow were investigated numerically and experimentally. The behaviors of column, penetration and breakup of plain liquid jet in non-swirling cross-flow of air have been studied. Numerical and physical models are based on a modified KIVAII code. The primary atomization is represented by a wave model based on the KH(Kelvin-Helmholtz) instability that is generated by a high interface relative velocity between the liquid and gas flows. CCD camera has been utilized in oder to capture the spray trajectory. The nozzle diameter was 0.5 mm and its L/D ratios were between 1 and 5. Numerical and experimental results indicate that the breakup point is delayed by increasing gas momentum ratio, the penetration decreases by increasing Weber number and the turbulent or nonturbulent liquid jet is obtained at different L/D ratio.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.45 no.6
• /
• pp.447-454
• /
• 2017

A three-dimensional two-phase large eddy simulation(LES) has been conducted to investigate the breakup and atomization of liquid jets such as a diesel jet in parallel flow and water jet in cross flow. Gas-liquid two-phase flow was solved by a combined model of Eulerian for gas flow and Lagrangian for a liquid jet. Two stochastic breakup models were implemented to simulate the liquid column and droplet breakup process. The penetration depth and SMD(Sauter Mean Diameter) were analyzed, which was comparable with the experimental data.

• Transactions of the Korean Society for Noise and Vibration Engineering
• /
• v.20 no.3
• /
• pp.287-295
• /
• 2010

This study deals with the design of a bi-directional damper using a tuned liquid damper(TLD) and a tuned liquid column damper(TLCD) for a SDOF building. Two dampers are usually needed to reduce wind-induced responses of tall buildings since they are along and across wind ones. The proposed damper has the advantage of controlling both responses with a single damper. The damper used in this study behaves as both a TLCD in a specific translational direction and a TLD in the other orthogonal direction. This paper presents experimental verification to confirm its control performance. First, shaking table test is carried out to investigate reducing responses by the damper. Control performance of the damper is expressed by the transfer function from shaking table accelerations to SDOF building ones. Testing results show that the damper reduced bi-directional responses of a SDOF building. Also, it reduced torsion responses.

• Journal of Electrical Engineering and Technology
• /
• v.10 no.5
• /
• pp.2205-2210
• /
• 2015

Experimental studies have been carried out to study the principle operation of the conductive type wire-mesh tomography sensor and analyse the wire-mesh tomography sensor for the liquid/gas two-phase flow interface and void fraction distribution in a process column. The measurement of the two-phase flows in the process column is based on the cross-sectional local instantaneous conductivity. The sensor consists of two planes of parallel electrode wires with 16 electrodes each and was placed orthogonally with each plane. The sensor electrode wires were made of tinned copper wire with an outer diameter of 0.91 mm which stretched over the sensor fixture. Therefore, this result in the mesh grid size with 5.53×5.53mm². The wire-mesh sensor was tested in a horizontal liquid/gas two-phase flows process column with nominal diameter of 95.6 mm and the sampling frequency of 5882.3529 Hz. The tomogram results show that the wire-mesh tomography provides significant results to represent the void fraction distribution in the process column and estimation error was found in the liquid/gas interface level