• 대한지역사회영양학회지
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• 제15권1호
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• pp.61-72
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• 2010

The purpose of this study was to evaluate the effect of a weight control program on anthropometric values, serum lipid profiles and nutrient intakes. The subjects of this study were 38 obese children (boy : 17, girl : 21) with obesity index over 120%. The weight control program for obese children included nutritional education (50 min) and exercise (50 min) for 10 weeks. The nutrition intakes of the children were surveyed before and after the weight control program by 24hr recall test. The BMI, R$\ddot{o}$hrer Index, Obesity Index, WHR (Waist - Hip ratio) and body fat(%) were significantly decreased after completion of the weight control program. The total cholesterol and HDL-cholesterol of subjects were significantly increased after the weight control program. Distribution of serum lipid profiles was slightly changed. The energy intakes were significantly decreased (p < 0.001). from 1760.8 kcal to 1435.2 kcal. In addition, the intakes of P, Zn, retinol, vitamin $B_1$, vitamin $B_2$, vitamin E and niacin were significantly decreased. while intakes of vitamin C and folate were increased. Calcium and folic acid were upper 25% of subjects, under EAR(Estimated Average Requirements) intake before the weight control program. The distribution of energy intakes was significantly changed into positive status; fat percentage was decreased 26.3% to 22.1% (p < 0.01). Carbohydrate was increased 58.6% to 61.2% (p < 0.05). Meal distribution of energy intakes was changed; calorie percentage from lunch significantly increased from 32.2% to 38.3%. Calorie percentage from snack significantly decreased from 17.7% to 13.5%. In conclusion, weight control program for 10 weeks is effective in obesity index and nutrient intakes although serum lipid values were a little changed.

• The Korean Journal of Ecology
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• 제22권2호
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• pp.89-94
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• 1999

Thirty-day-old seedlings of tomato (Lycopersicon esculentum) were treated with different concentrations of HgCl$_2$(0. 10 and 50 $\mu$M) for up to 20 days. and the detailed distribution of Hg absorbed and its toxicity in different plant parts (roots, stems and leaves) were investigated. The accumulation of Hg in plants increased with external Hg concentrations. and Hg is strongly retained by roots. Further. Hg content in leaves was various. showing more accumulation in older leaves. Seedlings exposed to toxic levels of Hg showed not only the reduction of dry weight and length of both shoot and root. and chlorophyll levels in leaves but also the enhancement of malondialdehyde (a lipid peroxidation product) formation in all plant parts investigated. These results suggest that physiological impairment of a plant exposed to Hg may be achieved by internal distribution of Hg absorbed and Hg-induced oxidative stress in different plant parts.

• Journal of Plant Biology
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• 제35권3호
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• pp.219-227
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• 1992

지질성 종자(해바라기, 피마자, 잣나무)와 전분성 종자(완두, 옥수수)를 대상으로 배유와 자엽세포내의 저장지질의 형성, 분포 및 구조적 변화 등과 지질가수분해효소의 활성부위 및 세포내 분포양상 등을 세포화학적 방법을 이용하여 조사하였다. 채종후의 지질 및 전분성 종자의 배유와 저장성 자엽세포에는 구형의 단백과립과 지질소구인 스페로솜, 전분과립 등의 저장물질이 널리 분포하였으며 세포내소기관은 드물게 관찰되었다. 활면소포체에서 형성되어 방출된 소포들과 스페로솜의 초기 단계로 여겨지는, 전자밀도가 낮은 막성의 과립들은 염색상이 스페로솜의 그것과 동일하였다. 조면소포체에서 방출된 전자밀도가 높은 과립들은 원형질막의 인접부위에서 관찰되었다. 지질염색반응 결과, 일반적인 미세구조의 염색상과는 상이하게 단백과립내의 단백질보다는 구형의 스페로솜의 전자밀도가 높고 균일함이 확인되어 스페로솜의 주요 구성성분은 지질임을 알 수 있었다. 스페로솜과 활면소포체에서 방출하는 물질을 함유한 소포는 염색상이 동일하였다. 지질가수분해효소는 분해과정이 진행중인 스페로솜의 기질과 막 주변부, 그리고 원형질막 부근에서 강한 활성을 보였다.

• BMB Reports
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• 제33권6호
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• pp.476-482
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• 2000

In this paper, we report a new cationic lipid composed of L-lysinamide and cholesterol as a potent gene delivery vector. $3{\beta}$[L-Lysinamide-carbamoyl] cholesterol could self-assemble with plasmid DNA forming discrete lipoplexes. From atomic force microscopic images of the complexes, the size distribution was observed to range from 100 to 150 nm in diameter. The transfection efficiency of this amphiphile on different cell lines was evaluated as a micellar solution in the absence of the fusogenic helper lipid, dioleoyl phosphatidyletbanolamine (DOPE). Transfection experiments were performed as a function of charge ratio (lipid/DNA) and transfection time. Cytotoxicity and in vitro transfection efficiency of the amphiphile was demonstrated and compared with those of commercially available Lipofectin and polyethylenimine (PEI).

• 한국식품과학회지
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• 제27권3호
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• pp.290-293
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• 1995

감자 lipoxygenase가 밀가루반죽의 지질분포, 지방산조성, 카로테노이드 및 색도 등의 화학적 성분 변화에 미치는 영향을 연구하기 위해 감자 lipoxygenase를 밀가루 g당 $6.5{\times}10\;unit$ 수준으로 첨가하여 실험하였다. 밀가루에 감자 lipoxygenase를 첨가하므로서 밀가루 반죽의 과산화물가가 증가하고 지방산조성 중 linoleic acid의 현저한 감소와 함께 유리지질함량이 증가하였다. 효소의 첨가에 의한 유리지질의 증가는 효소에 의한 불포화지방산의 산화와 관련이 있는 것으로 보여진다. 또한 lipoxygenase 첨가에 의해 밀가루 반죽내의 카로테노이드 함량이 낮아져서 효소에 의한 탈색효과가 관찰되었다. 색차계에 의해 측정된 색도 역시 효소첨가로 밀가루 반죽의 적색도와 황색도가 낮아져서 색깔이 옅어졌다.

• 사상체질의학회지
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• 제12권2호
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• pp.171-183
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• 2000

1. Back ground and purpose An experimental study has done to examine the effect of defense and cure gastric mucasal damage of Mokdan-pijihwang-tang. 2. Methods Mice had intragastric injected with MJ extract before indomethacin treatment which induces homorrhage infarct and erosion artificially. Degree of lipid peroxidation, general morphology, change of mucous cell, the distribution of PNA, ICAM and distribution of apoptotic cell were objected. (Abbreviation) MJ : Mokdanpijihwang-tang, PNA : Peanut Agglutinin, ICAM : Intercellular Adhesion Molecule 3 Results 1) The degree of lipid peroxidation in INDO-group had increased conspicuously than control group. But the degree of lipid peroxidation in MJ-group had decreased than INDO-group and these decline had probability. 2) After indomethacin treatment, hemorrhage infarct and erosion had increased in stomach body. But in MJ-group, the configuration is normal, except the group intragastric injected with MJ extract at hour 24 before indomethacin treatment. 3) Surface mucous cell and neck mucous had disappeared in INDO-group. But in MJ-group tormal distribution had shown like control group except the group intragastric injected with MJ extract at hour 24 before indomethacin treatment. 4) PNA positive reaction had not shown in INDO-group. But medium PNA positive reaction had shown In Mj-group. 5) ICAM positive reacted cell had shown in INDO-group. The decrease of ICAM positive cell were shown than INDO-group. 6) A number of apoptotic cell was distributed in hemorrhagic erosion. A few number of apoptotic cell was distributed in MJ-group except some surface mucous. 4. Conclusion These results suggest that MJ has an effect on cure of gastric mucosal damage.

• 운동영양학회지
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• 제13권1호
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• pp.9-14
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• 2009

The effects of a hypocaloric diet with or without strength training on body fat distribution and serum lipid concentrations in obese elderly women were investigated. Twenty-six healthy women (age 66±4.6 yr; body mass index 32.3±2.9 kg/m²) were randomly assigned to 3 groups: control (C; n=8), hypocaloric diet (DO; n=9) or hypocaloric diet with strength training (DST; n=9). Subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT), and inter and intra muscular adipose tissue (IMAT) were measured using magnetic resonance imaging. Serum lipid concentrations including total cholesterol (TC), high density lipoprotein cholesterol (HDLC), low density lipoprotein cholesterol (LDLC), and triglycerides (TG) were measured. No significant changes occurred in body weight and percent body fat in the C group over the 16-week period. The DO and DST groups lost similar amounts of weight and fat after 16 weeks. SAT and VAT decreased after weight loss in the DO and DST groups but not in the C group. IMAT was significantly reduced in the DO and DST groups, whereas in the C group IMAT increased. The loss in IMAT mass was similar in the DO and DST groups. TC and LDLC decreased in the DO and DST groups but not in the C group. There were no differences between the DO and DST groups in decrease in TC and LDLC. HDLC decreased in the DO group but not in the C and DST groups. TG tended to decrease in the DST group. In conclusion, body fat distribution including SAT, VAT, and IMAT and serum lipid concentratons were modulated by weight loss resulting from the hypocaloric diet with or without strength training in obese elderly women. Strength training did not enhance the improvement in body fat distribution and serum TC and LDLC concentrations by the hypocaloric diet.

• 대한한방내과학회지
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• 제19권2호
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• pp.60-80
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• 1998

In 123 examples of admission patients at Dongshin university oriental medical hospital circulatory internal medicine, we sequently investigated serum lipid, at admission state, 2th and 4th weeks. The results were as follows; 1. In the case of 123 stroke patients, only 12cases were related to Hyperlipemia. So stroke was not related to Hyperlipemia significantly. 2. According to sex, woman has more significant results in the T-cholesterol and LDL-cholesterol. 3. According to the living Zone, Triglyceride of the city zone group washigher than that of the contryside zone significantly. 4. According to the history of drinking and smoke, the patients who have the history of drinking and smoke had a significant remark in T-cholesterol and LDL-cholesterol. 5. In the case of patients like eating meat, it was significant gain of HDL-cholesterol. 6. In the patients who had past history of Hypertension, the value of Triglyceride is significantly low, and sequently as blood pressure decreased, serum lipid value decreased too. 7. In the patients who had past history of D.M., the value of Triglyceride is significantly high, and sequently as blood sugar value decreased, serum lipid value decreased too. 8. In the case of patients treated He-Ne laser, Triglyceride was decreased significantly. 9. In the case of treating the stroke patients, serum lipid had non-significant decrease value.

• 생명과학회지
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• 제16권6호
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• pp.955-959
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• 2006

단백질의 serine 및 threonine 잔기에 O-linked N-acetylglucosamine (O-GlcNAc)의 수식은 핵단백질과 세포질 단백질의 주요 조절인자로 부각되고 있다. 본 연구에서는 GlcNAc를 인산화시켜 GlcNAc 6-phosphate로 만드는 GlcNAc kinase (NAGK, EC2.7.1.59)의 세포내 표현을 면역화학적 방법으로 조사하였다. 배양한 해미신경세포에서 NAGK는 가지돌기를 따라 점박이(punctae)를 형성하였으며, 이 점박이들은 caveolin-1 혹은 flotillin 항체에도 염색이 되었다. 이들은 각각 caveolac와 lipid raft의 표지단백질이기 때문에 본 연구결과는 NAGK가 세포막의 이러한 특수 미세부분(microdomain)에 존재함을 의미하며, 이 미세부분에서 아직 알려지지 않은 어떤 기능을 할 것을 시사한다.

• 한방안이비인후피부과학회지
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• 제16권3호
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• pp.96-115
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• 2003

Introduction and Objectives : Administrating the Sopungchengyoungtanggami-bang (SCG) extract on to the atopic dermatitis(AD) is to study change of external dermal formation, change of leukocytes in vasculature, change of lipid formation in stratum corneum and distribution of ceramide and this study is done through forcing injury to rat's back skin which are lipid protect formation in stratum corneum. Materials and Methods : The AD which caused intentionally using the external application on the rat's back skin was used the SCG. The change of leukocytes in vasculature has been identified through optima 5.2 and student t-test and the results were made into dermal formation graph. Results : After dispensing SCG extract into the AD, the dermal injury was decreased. Especially, recover of lipid protection formation which include lipid and ceramide in stratum corneum is suppressing acute inflammation that some factors are PKC, TNF-${\alpha}$, IL-12B which controled the secretion of relating inflammatory cytokine, also went onto decrease of angiogenesis, and the decrease of degranulated mast cell. In addition, the decrease of epithelial injury also caused the growth of cell to decrease in stratum basale and cytoclasis. In the vasculature, the leukocytes were also decreased and it could relate to decrease AD Conclusions : Thus, SCG has effect on AD suppressing the dermal injury through recovering of lipid protection formation in stratum corneum.