• Title/Summary/Keyword: lipid composition

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Stratification of Lipid Content and Composition in Blubber of Marine Cetacean from Korean Waters (한국 근해에 서식하는 고래 피하지방의 층별 지방 함량 및 구성 변화)

  • Ko, Ah-Ra;Ju, Se-Jong;Moon, Dae-Yeon;Choi, Seok-Gwan;Kim, Zang-Geun;Shin, Kyung-Hoon
    • Ocean and Polar Research
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    • v.33 no.1
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    • pp.35-43
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    • 2011
  • Blubber plays different biological roles in cetaceans, including structural support, physiological demands (thermoregulation, streamlining, buoyancy, etc.), and energy storage. As such, biochemical composition, especially lipid, in the blubber may be vertically stratified in order to effectively assist with these functions. We analysed lipid content, class and fatty acid composition in the blubber of by-catch minke whale (Balaenoptera acutorostrata; n = 11), long-beaked common dolphin (Delphinus capensis; n = 3) and Pacific white-sided dolphin (Lagenorhynchus obiquidens; n = 2) from Korean waters. Lipid content for all three species ranged from 55 to 96% of wet weight, with a dominance of triacylglycerols. Vertical stratification of blubber lipid content was different between species. For minke whale and longbeaked common dolphin, it increased from inner (near the muscle) to outer (near the skin) layer, whereas it decreased for Pacific white-sided dolphin. Thirty-one fatty acids were found in all blubber samples and vertical stratification of fatty acid composition was similar among all three species. Among grouped fatty acids, monounsaturates were the most abundant, gradually increasing from inner to outer blubber layer, whereas polyunsaturates and saturates decreased. Such vertical stratification of lipid content and composition suggests that the modification and accumulation of lipid occur in the blubber itself in order to help cetaceans with their specific physiological needs.

Studies on Lipid Intake, Serum Lipid Profiles and Serum Fatty Acid Composition in College Women Who Smoke (흡연여대생의 지질섭취와 혈중지질 및 지방산 조성에 관한 연구)

  • 안홍석;이금주;김나영
    • Korean Journal of Community Nutrition
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    • v.7 no.1
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    • pp.102-110
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    • 2002
  • Cigarette smoking has long been recognized as a major risk factor in the development of coronary heart disease. Several investigators have reported the strong association between smoking and high serum cholesterol, triglyceride concentration, SFA and low HDL cholesterol, PUFA and $\omega$6 concentrations. Therefore, this study was done to investigate the effect of smoking on the serum lipid profile and fatty acid composition of college women. Sixty-one non-smokers and twenty-seven smokers were selected from college women students in the Seoul area. Their lipid intake, serum lipid concentration and fatty acid composition were examined. There were no differences in the general characteristics and anthropometric indices between the smokers and non-smokers. However, alcohol consumption was significantly higher in smokers than non-smokers (p<0.001). The daily caloric intake of smokers and non-smokers were 1875.84 kcal and 1915.53 kacl, respectively. On the other hand, the mean daily intake of lipids and cholesterol were significantly lower in smokers (p<0.05). In smokers, the mean concentrations of serum total cholesterol, LDL-cholesterol and the LDL-C/HDL-C ratio were higher, and the compositions of EPA and DHA were lowe than in non-smokers. There was a negative correlation between the serum triglyceride and PUFA levels in the two groups. Also, serum HDL-C correlated negatively with MUFA in smokers and non-smokers (p<0.01). These results suggest that smoking cause inadequate changes in serum lipid profile and serum fatty acid composition, thereby increasing the tendency for coronary heart disease.

Fatty Acids Composition in Viscera and Muscle of the Philippines Pinkgray Goby, Amblvchaeturichthys hexanema

  • Choi Byeong-Dae;Kang Seok-Joong;Jeong Bo-Young;Mendoza Lonarda S.
    • Fisheries and Aquatic Sciences
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    • v.4 no.4
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    • pp.265-267
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    • 2001
  • In this study, lipid classes and fatty acids composition in viscera and muscle of the philippines Pinkgray goby, Amblychaeturichtys hexanema were determined. The viscera contained high levels of total lipid $(TL,\;4.8\%)$, while the muscle contained less TL $(0.5\%)$. TL in the viscera consisted of triacylglycerol $(83.9\%)$, diacylglycerol $(7.9\%)$, polar lipid $(7.4\%)$, and cholesterol $(0.8\%)$. However, the muscle contained a small amount of TG $(3.0\%)$ and much more PL $(73.8\%)$. Principal fatty acids composition of PL and NL in the viscera and muscle were 16:0, 16:1n-7, 18:0, 18: 1n-9, 18:1n-7, l8:3n-3, 20:5n-3, 22:5n-3, and 22:6n-3.

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Effects of cheungho aqua-acupunctureat gansoo$(BL_{18})$ and illweul$(GB_{14})$ on plasma lipid composition in rat fed high fat diet (간유(肝兪) 및 일월(日月) 청호약침(靑蒿藥鍼)이 고지방(高脂肪) 급여(給與) 흰쥐의 혈청(血淸) 지질구성(脂質構成)에 미치는 영향(影響))

  • Lee Joon-Moo
    • Korean Journal of Acupuncture
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    • v.20 no.3
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    • pp.91-99
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    • 2003
  • Effects of cheungho aqua-acupuncture at gansoo$(BL_{18})$ and illweul$(GB_{14})$ on plasma lipid composition were investigated in rat fed high fat diet. Body weight gain showed a tendency to decrease in aqua-acupuncture groups and in aqua-acupuncture groups, gansoo and illweul join aqua-acupuncture group showed a lower values than other aqua-acupuncture groups. Concentrations of plasma triglyceride, free fatty acids, lipoprotein and glucose showed a tendency to decreasea in the aqua-acupuncture groups. However in aqua-acupuncture groups, these values showed no significantly different. In plasma lipid composition, total cholesterol and LDL- cholesterol showed a tendency to decrease in aqua-acupuncture groups, however the values of LDL-cholesterol showed no significantly different in the aqua-acupuncture groups. HDL-cholesterol concentration showed a tendency to increase in aqua-acupuncture groups and in aqua-acupuncture groups, gansoo aqua-acupuncture group showed a high value.

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The Effects of Nitrite Treatment on the Lipid Composition, Fatty Acid Composition, and Susceptibility to Oxidation of Pork Biceps Femoris Muscle

  • Han, S.K.;Yamauchi, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.12
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    • pp.1764-1769
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    • 2000
  • The purpose of this experiment was to investigate the antioxidant effect of nitrite on total, neutral, and polar lipids and fatty acid composition in laboratory-cooked ground pork. Muscle samples (Biceps femoris) were analyzed using Iatroscan, gas chromatography, phosphorus content, and TBARS value. The total and neutral lipid contents of muscle were higher in nitrite-untreated meat (0 ppm) than in nitrite-treated meat (100 ppm) but the reverse was observed for polar lipid contents. The results for neutral lipids showed a similar trend when compared with total lipids. Polyunsaturated fatty acids contents of total, neutral and polar lipid in 100 ppm treated meat were higher than that of 0 ppm. The phosphorus content was higher in 100 ppm meat than in 0 ppm but the reverse was observed for TBARS value. These results showed that the addition of 100 ppm nitrite to ground pork resulted in a remarkable antioxidant effect during refrigeration storage.

Thermal Acclimative Changes in the Different Lipid Fractions Composition of Fat Body of Eri-Silkworm, Philosamia Ricini (Ward.)

  • Singh, G.B.;Singh, M.K.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.4 no.1
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    • pp.13-17
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    • 2002
  • Present communication deals with quantitative determination of total lipid, triglycerides, total free fatty acids, phospholipids and total cholesterol in the fat body tissue of the silkworm adapted to low and high temperatures. At the end of spinning process is characterized by a marked cellular reorganization of the different lipid fraction of the fat body irrespective of thermal acclimation. Accordingly, the per cent composition of triglycerides of the total lipid is increased accompanied by a corresponding decrease in free fatty acids, phospholipids and cholesterol.

Changes of Protein and Lipid Composition During Germination of Perilla frutescens Seeds (들깨 종자의 발아에 따른 단백질 및 지방의 조성 변화)

  • 정대수;김현경
    • Journal of Life Science
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    • v.8 no.3
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    • pp.318-325
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    • 1998
  • To investigate changes in protein and total lipid contents, seed storage protein pattern, and fatty acid composition of germination perilla(Perilla frutescens) seeds. Also, the corresponding value components in cotyledons, hypocotyles and roots were measured according to germination stage. The results were summarized as follows ; During germination, pertein and total lipid contents of Yepsilldalggae and Kwangyang cultivar were decreased continuously. In particular, protein contents rapidly decreased to the 3 days after germination(DAG), and then total lipid contents rapidly decreased between 3 DAG and 10 DAG. In changes of protein and total lipid contents of cotyledons, protein contents of Yeupsildalggae was increased during the germination, but Kwangyang cultivar was decreased during the same periods. The total lipids contents of Yeupsildalggae and Kwangyang cultivar were decreased during the germination. According to SDS-PAGE analysis, there was no detectible polypeptide bands on the gel before seed germination suggesting that this may be due to the rapid degradation of the storage proteins in the mature seed by hydrolyttic enzymes during the stage. During germinatation , the polypeptide band with 27$\sim$28KD of Yeupsildalggae and Kwangyang cultivar were accumulated gradually. In changes of fatty acid composition of total lipid of Yeupsildalggae and Kwangyang cultivar , saturated fatty acids such as palmitic acid and stearic acid increased during the germination. On the other hand, unsaturated fatty acid such as linoleic acid and linolenic acid decreased during the same periods. However, oleic acid increased to the 5 DAG, and then was repidly decreased.

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Composition of Lipid and Amino Acid in Semisulcospira gottschei Tissues (다슬기중 지방질 및 아미노산 조성)

  • 심태흠;한규석;이태준;정의호;이해금
    • Journal of Food Hygiene and Safety
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    • v.9 no.2
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    • pp.81-87
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    • 1994
  • This study was performed to investigate the detailed lipid content, lipid composition and amino acid composition of Semisulcospira gottschei tissues. Lipids of Semisulcospira gottschei tissues were extracted by the mixture of chloloform-methanol, fractionated into neutral lipids, glycolipids and phospholipids by silicic acid column chromatography and the composition of these lipid classes were determined by TLC and GLC. the amino acids in Semisulcospira gottschei tissues was analyzed by the amino acid auto analyzer. The total lipids content was 1.4% and the main components of the total lipids were neutral lipids 67.9%, glycolipids 19.3% and phospholipids 12.8%, respectively. The main fatty acids of total lipids were palmitic acid (20.5%), palmitoleic acid (16.45) and linolenic acid+eicosenoic acid (15.0%) and linoleic acid(13.1%), the main fatty acids of glycolipids were palmitic acid (41.9%), palmitoleic acid (19.7%) and oleic acid (11.7%), and the main fatty acids of phospholipids were linolenic acid+eicosenoic acid (55.1%), oleic acid (17.3%) and palmitic acid (11.4%). The main amino acids were glutamic acid (16.0%) and aspartic acid (11.1%).

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Fatty Acid Composition and Electrophoretic Patterns of Proteins of Korean Soybeans (우리나라 콩의 지방산 조성 및 단백질의 전기영동 패턴)

  • Kim, Jong-Goon;Kim, Sung-Kon;Lee, Joon-Shik
    • Korean Journal of Food Science and Technology
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    • v.20 no.2
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    • pp.263-271
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    • 1988
  • Fatty acid composition and electrophoretic patterns of protein of Korean soybeans were studied, The protein content showed an inverse relation with the lipid content, The content of lipid types of total lipid was in the order of neutral lipid, glycolipid and phospholipid, Cultivar 102-B had higher content of neutral lipid but lower content of phospholipid compared with others. The main component of neutral lipid was triglyceride followed by sterol. The main fatty acids of total lipid were linoleic, oleic and palmitic acids, which comprised over 85% of total fatty acids. The main fatty acid of neutral lipid, glycolipid and phospholipid were linoleic acid. The polyacrylamide gel electrophoretic pattern of protein showed seven bands. The cultivar 102-B had the highest content of IIS globulin. Sodium-dodecyl sulfate PAG electrophoresis demonstrated that all soybeans had similar composition of subunit.

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Age Related Changes of Microsomal Fatty Acid Composition and Lipid Peroxidation in 2-acetylaminofluorene Treated rats. (2-Acetylaminofluorene의 투여와 나이에 따른 쥐의 세포막 지방산 조성 및 지질과산화물 생성의 변화)

  • 윤은영;최혜미;김현아;김숙희
    • Environmental Mutagens and Carcinogens
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    • v.15 no.2
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    • pp.140-147
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    • 1995
  • For studying the effect of different dietary fats on carcinogenesis, fatty acid composition of membrane and lipid peroxidation were measured. Male Sprague-Dawley rats were fed on diet containing 15%(w/w) beef tallow or soybean oil. A single dose of 50 mg 2-AAF/kg B.W. was injected i.p. in each diet group 10 times. Rats were sacrifled after 1, 5, 10, and 15 weeks from the first injection. By 2-AAF injection, !ipid peroxidation increased slightly compared to control group. The rats fed on different fats had similar MDA production and those fed on soybean oil had slightly higher free radical concentration measured by ESR. In young rats, iipid peroxidation level was high and hydroxy radical production was higher in soybean oil group than in beef tallow group. With age, the lipid peroxidation values were decreased initially then increased. The fatty acid composition in microsomal membrane was reflected by dietary fatty acid composition. In soybean oil group, monoenoic acid was lower and polyunsaturated fatty acid was higher than beef tallow group. Linoleic acid contents showed the most discrepancy among groups. By 2-AAF treatment, iinoleic acid content and unsaturation index increased in soybean oil group. But in beef tallow group, there was no difference in fatty acid contents.

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