• Journal of Veterinary Science
• /
• v.22 no.4
• /
• pp.45.1-45.13
• /
• 2021

Background: Peste des petits ruminants (PPR) is an infectious disease caused by the peste des petits ruminants virus (PPRV) that mainly produces respiratory symptoms in affected animals, resulting in great losses in the world's agriculture industry every year. Single-domain variable heavy chain (VHH) antibody fragments, also referred to as nanobodies, have high expression yields and other advantages including ease of purification and high solubility. Objectives: The purpose of this study is to obtain a single-domain antibody with good reactivity and high specificity against PPRV. Methods: A VHH cDNA library was established by immunizing camels with PPRV vaccine, and the capacity and diversity of the library were examined. Four PPRV VHHs were selected, and the biological activity and antigen-binding capacity of the four VHHs were identified by western blot, indirect immunofluorescence, and enzyme-linked immunosorbent assay (ELISA) analyses. ELISA was used to identify whether the four VHHs were specific for PPRV, and VHH neutralization tests were carried out. ELISA and western blot analyses were used to identify which PPRV protein was targeted by VHH2. Results: The PPRV cDNA library was constructed successfully. The library capacity was greater than 2.0 × 10⁶ cfu/mL, and the inserted fragment size was approximately 400 bp to 2000 bp. The average length of the cDNA library fragment was about 1000 bp, and the recombination rate was approximately 100%. Four single-domain antibody sequences were selected, and proteins expressed in the supernatant were obtained. The four VHHs were shown to have biological activity, close affinity to PPRV, and no cross-reaction with common sheep diseases. All four VHHs had neutralization activity, and VHH2 was specific to the PPRV M protein. Conclusions: The results of this preliminary research of PPRV VHHs showed that four screened VHH antibodies could be useful in future applications. This study provided new materials for inclusion in PPRV research.

• International Journal of Knowledge Content Development & Technology
• /
• v.14 no.3
• /
• pp.59-76
• /
• 2024

The prominent role accorded to social media in the academic community for research, teaching and learning revolves around its significance among users. Social media offers a platform for individuals to engage with and share perceptions relating to different disciplines. This current research was conducted to investigate the level of awareness and frequency of social media technology use among postgraduate students of Library and Information Science in Nigerian universities. The descriptive survey design was used for the study. Structured questionnaires were used to collect data from 919 library and information science (LIS) postgraduate students in the universities. In all, 742 copies out of the 919 distributed were returned and found usable, thereby making the return rate to be 81%. Data collected were analysed using mean and standard deviation. The study revealed that the LIS postgraduate students frequently use social media such as Wikipedia (x=3.94>3.50), Instagram (x=3.86>3.50), Facebook (x=3.85>3.50), Zoom ($\overline{x}$=3.78>3.50), LinkedIn (x=3.69>3.50), YouTube ($\overline{x}$=3.54>3.50), Twitter (x=3.52>3.50). The study established that students use social media tools for their personal, professional and research activities. The study also found that the level of awareness and use of social media by the students was high. The study recommended that the use of social media should be incorporated into the LIS curriculum including training sessions for the students on how to use the media effectively.

• Journal of Korean Library and Information Science Society
• /
• v.27
• /
• pp.271.2-336
• /
• 1997

The present study is a review to determine by what bodies and through what mechanisms the national industrial information policy is implemented and administered, that is what is its content and how it is linked to industrial and technological needs in the field of industrial firms. There are lacking essential capabilities required to transfer industrial information and to channel it to ultimate users. It is not possible to identify users' information needs adequately, to acquire pertinent information, to adapt it to prevailing conditions, to disseminate it to those who need it. Various organizations work to meet the specific information requirements of industry. These organizations include governmental agencies, industrial associations, research institutes and information centers. They have, however, developed independently without liaison among them. They may be some unnecessary duplication in some spheres and inadequate facilities in others. A n.0, ppropriate institutions which provide industrial information would be industrial associations, industrial research and development institutes. They serve as a coupling mechanism between industry and the existing information facilities.

• Journal of Korean Library and Information Science Society
• /
• v.16
• /
• pp.99-128
• /
• 1989

The purpose of this study is to examine the problems of the each cataloging rules about the physical description area that generated the greatest controversy in the cataloging of microcomputer software, and to suggest solutions of these problems. The results of the study can be summarized as follows: 1. Because the physical description area of materials is to identify the physical attributes of the carrier, file description might better described in the material specific details area. 2. Integrated software that related file is linked together need to be used terms that represented any type of software 3. It is desirable that the term 'computer' is to be used as a modifier to devide the carrier of microcomputer and other non book materials. 4. System requirements would better described in a note area rather than physical, description area. 5. It is desirable that other physical details such as recording density, tracks, etc., is to describe in the physical description area, since such information is to represent specifics of the carrier.

• Journal of Korean Library and Information Science Society
• /
• v.30 no.3
• /
• pp.129-150
• /
• 1999

The present study is a review to determine by what bodies and through what mechanisms the information resources management is implemented and administered, that is its content and how it is linked to industrial information units in the field of industrial firms. Industrial information units are lacking essential capabilities required to acquire internal information and to organize sharing information which is intergrated to internal and external information. Various units within firms work to implement the information resources management. These units include management information systems, information center, and newly developed mission oriented units. They have, however, developed without liasison industrial information units. They may be some unnecessary duplication in some spheres and inadequate facilities in others.

• Journal of the Korean Society for Library and Information Science
• /
• v.32 no.3
• /
• pp.251-264
• /
• 1998

The purpose of this study is to build a Web Based IT Trend Retrieval System using Visual Foxpro. In this system, we can retrieve recent titles of Information Technology Trend articles. Besides, all of the titles are linked to the contents of the articles. For this purpose, FoxWeb and Personal Web Server was utilized also.

• Proceedings of the Korean Information Science Society Conference
• /
• 2010.11a
• /
• pp.38-39
• /
• 2010

• Journal of Microbiology and Biotechnology
• /
• v.12 no.3
• /
• pp.508-513
• /
• 2002

Human endogenous retroviral long terminal repeats (LTRs) have been found to be coexpressed with sequences of genes located nearby. It has been suggested that the LTR elements have contributed to the structural change or genetic variation of human genome connected to various diseases. The HERV-W family has been identified in the cerebrospinal fluids and brains of individuals with schizophrenia. Using a cDNA library derived from a human brain, the HERV-W LTR elements were examined and five new LTR elements were identified. These elements were examined using a YAC clone panel from the Xq21.3 region linked to psychosis that was replicated on the Y chromosome after the separation of the chimpanzee and human lineages. Fourteen elements of the HERV-W LTR were identified in that region. Those LTR elements showed a high degree of sequence similarity ($91.8-99.5\%$) with previously reported HERV-W LTR. A phylogenetic tree obtained from the neighbor-joining method revealed that new HERV-W LTR elements were closely related to the AXt000960, AF072504, and AF072506 from the GenBank database. The data indicates that several copy numbers of the HERV-W LTR elements exist on the Xq21.3 region and are also expressed in the human brain. These LTR elements need to be further investigated as potential leads to neuropsychiatric diseases.

• Bulletin of the Korean Chemical Society
• /
• v.34 no.2
• /
• pp.460-464
• /
• 2013

Single-chain variable fragments of antibodies (scFv) specific to 2,4-dinitrotoluene (DNT) were isolated from a phage library displaying synthetic human scFv fragments with 6 diversified complementary determining regions (CDRs). A DNT derivative that contained an extended amine group was synthesized and conjugated to the NHS-group that was linked to magnetic beads. Phages specific to the immobilized DNT derivatives were isolated from the library after 4 rounds of sequential binding and elution processes. The displayed scFv fragments from the isolated phages showed consensus CDR sequences. One DNT-specific scFv was expressed in E. coli and purified using Ni-affinity chromatography. The purified DNT-specific scFv binds specifically to the immobilized DNT-derivative with $K_D$ value of $6.0{\times}10^{-7}$ M. The scFv and DNT interaction was not disrupted by the addition of 4-nitrotoluene or benzoic acid. These data demonstrate that the screened scFv from the phage displayed library could be used for selective and sensitive detection of explosives such as TNT.

• Journal of the Korean Society for Library and Information Science
• /
• v.53 no.2
• /
• pp.247-266
• /
• 2019

The purpose of this study is to propose a W3C standard, DCAT, to manage and service dataset that is becoming increasingly important as new knowledge information resources. To do this, we first analyzed the class and properties of the four core classes of DCAT. In addition, I modeled and presented a system that can manage and service various data sets based on DCAT in digital library. The system is divided into source data, data set management, linked data connection, and user service. Especially, the DCAT mapping function is suggested in dataset management. This feature can ensure interoperability of various datasets.