• Journal of Acupuncture Research
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• 제17권2호
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• pp.187-208
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• 2000

By the activation of ovary hormone, many morphological changes occur in the epithelial cell lines and muscle cells in rat uterus. These two cells in uterus are important to the implantation of embryo, maintaining pregnancy and starting parturition. One important change associated with the morphological change of these two cells in uterus is the change on prostaglandin(PG) metabolism. Its presence and synthesis in endometriurn and myometrium in uterus affects estrous cycle and the start of embryo implantation in uterus. It also performs as an important modulator in parturition. So the abnormally weak expression of PG causes difficulty during labor and over-expression causes pre-term labor. PG biosynthesis starts from either free or liberated arachidonic acids from membrane phospholipid by phospholipase. Such arachidonic acids are converted into PG catalyzed by Cyclooxygenase. Under normal physiological condition, Cyclooxygenase-1(COX-1) having 602 units of amino acids controls the synthesis of PG. It acts as a local hormone regulating vasomodulation of blood flow, flexible muscle movement, increasing the blood permeability and contributing the protective role in preserving integrity of the stomach lining and Cyclooxygenase-2 (COX-2) is induced by the inflammation, pregnancy and increased its expression until parturition. Lipid metabolite like PG is located in uterine and expression of COX-2 increased with pregnancy. Increased expression of COX proteins in epithelial cells and myometrial cells are told to increase the muscle contractility in uterus but decreased right after the labor in rat. It is a good sign indicating that COX proteins are deeply related to the start of labor. Currently, Several studies report the use of PG and COX-2 inhibitor as medication for controlled abortion or to prevent pre-term labor but they entail various side-effects. Our study proposed to suggest use of acupuncture as an another mediator to control abortion or pre-term labor without causing unnecessary side-effects by those medicines. Two acupuncture sites, LI-4 & SP-6 were selected due to their known efficacy. From the immunohistochemical staining of COX-2, normal expression of COX-2 protein in nonpregnant SD rat's uterus revealed that COX-2 protein was primarily detected in the lumina epithelial lining and in the epithelial cell lining contacting the stromal cells. High resolution optical microscopic scanning revealed distinguishable staining in the myometrial mucosa. LI-4 acupuncture administered nonpregnant rat's uterus showed strong expression for COX-2 in endometrium contacted with lumina epithelial lining of rat uterus and in myometrial mucosa. Stromal cells showed more staining than untreated nonpregnant rat's uterus and stronger staining in stromal cells contacting myometrial layer compared to untreated nonpregnant rat's uterus. SP-6 acupuncture administered nonpregnant rat's uterus showed weak expression for COX-2 in myometrial layers and stromal cells but no staining was visible in lumina epitheliai and glandular epithelial cells. Few stromal cells and myometrial mucosa were positively stained for COX-2. Pregnant SD rat's uterus was also immunostained for COX-2 expression after 18 days of pregnancy. Unlike to untreated nonpregnant rat's uterus, luminal epithelial cells were not positively stained for COX-2 but stronger staining for COX-2 was revealed in stromal cells. LI-4 acupunctured SD rat's uterus had very strong expression of COX-2 in luminal epithelial lining. Few stromal cells showed stronger positive COX-2 staining and myometrial layers also showed more expression than untreated pregnant rat. SP-6 acupuncture administered pregnant SD rat's uterus showed positive expression of COX-2 in epithelial cells of luminal mucosa layer but weaker than that of LI-4 acupuncture treatment's case. However, strong positive staining was revealed in stromal mucosa and myometrial layers. Virgin SD rat's uterus motility index during LI-4 acupuncture was 66.52 % (Prob〉T = 0.0197) compared to its motility before the acupuncture treatment but the motility index was slighdy elevated up to 79.58 % (Prob〉T = 0.1175) after the acupuncture. During the SP-6 acupuncture treatment for 30 minutes, uterus motility index was 90.52 % (Prob〉T = 0.1832) showing lesser decrement but consequently reached similar motility index decreasal to 79.95 % (Prob〉T = 0.0215) after the acupuncture treatment as LI-4 showed. LI-4 acupuncture tend to be a quick treatment to reducing the uterus motility in a virgin rat but eventually both two acupuncture administration created very similar reduction of uterus motility seeing the index after the both acupunctures. The uterus movement monitored during the LI-4 acupuncture administered for 30 minutes, Pregnant SD rat showed decreased motility down to 77.90 % (Prob〉 T = 0.0076) compared to uterus motility before the acupuncture and it continuously decreased down to 71.81 %(Prob〉T = 0.0214) after the removal of needle. The statistical analysis using paired t-test showed significance difference for both two motility indexs at =0.05. SP-6 acupuncture administered to pregnant SD rat also had similar pattern of decreasing uterus motility index down to 74.70 % (Prob〉T = 0.1730) during the initial 30 minutes acupuncture administration and it was continuously lowered to 71.52 % (Prob〉T = 0.0155) after the acupuncture. The paired t-test resuit for SP-6 suggest prompt response of uterus motility index to the SP-6 acupuncture treatment but consequently reached same level of inducing the motility reduction as LI-4 at =0.05 level.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제32권5호
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• pp.418-425
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• 2006

Purpose: This study was aimed at evaluating the histological changes of new bone and expression of osteopontin (OPN) after mandibular distraction osteogenesis. Materials and Methods: Unilateral mandibular distraction (0.5 mm twice per day for 10 days) was performed in eight adult dogs. Two animals were sacrificed at 7, 14, 28 and 56 days after completion of distraction, respectively. The distracted bones and contralateral non-distracted control bones were harvested and processed for histological and immunohistochemical examinations. Results: The new bone was arranged to tension direction after distraction osteogenesis. 7 days after distraction, numerous osteoblasts lining the immature trabecular bone and fibroblast-like cells in the fibrous intrezone were observed. 14 days after distraction, the new formed trabecular bones were thickened compared with 7 days after distraction. 28 days after distraction, the fibrous interzone was almost filled with newly calcified bone, and it was more hardened at 56 days after distraction. Increased OPN signals detected in the osteoblasts lining the trabecular bone and fibroblast-like cells in the fibrous interzone at 7 and 14 days after distraction. At 28 days after distraction, the OPN was weakly expressed in the osteoblasts, and it was not detected in all cellular components of distracted bone at 56 days later of distraction. Conclusions: After distraction osteogenesis, the distracted zone was completely calcified during the 56 days of consolidation period. In this study, the staining intensity of OPN increased in the osteoblasts and fibroblast-like cells at 7 and 14 days after distraction. The expression pattern of this protein shown here suggested that OPN play an important role in the osteogenesis during the early consolidation period.

• 한국터널지하공간학회 논문집
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• 제9권2호
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• pp.171-182
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• 2007

도심지 개착구간의 교통 및 환경적인 영향을 최소화하기 위하여 단면적 $200\;m^2$ 이상의 정거장터널이 퇴적암반 중에 계획되었으나 토피고가 13 m 이하인 설계조건에 직면하게 되었다. 본 연구에서는 패턴설계가 아닌 시공사례와 아칭효과 발현을 기초로 세 가지 요소 - 단면형상의 영향, 작용하중의 적용방법, 지보방안과 터널안정성분석 - 를 중심으로 설계방향이 논의되었다. 단면형상의 역학적인 영향에 기초하여 기본설계안과 연구단면안이 유도되었고, 지보방안은 터널천반부의 침하방지 및 역학적인 평형상태를 유지하기위한 파이프루프 보조공법과 NATM의 지보원리를 활용하였다. 두 설계안의 비교분석으로부터, 터널라이닝을 개착구조물의 연장선상에서 제약한 설계조건과 터널안정성 및 철근배근의 시공성에 대해 기본설계안이 적합한 방안임을 확인할 수 있었다. 그리고 동일한 건축한계로 아치부의 응력집중이 발생되지 않는 안정적인 응력분포를 나타내는 연구단면안의 분석결과에서 보듯이 향후 대단면터널 설계기술의 향상을 위해 단면형상의 영향과 이완하중의 적용방안이 심도 있게 검토되어야 함을 알 수 있다.

• 헤리티지:역사와 과학
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• 제53권3호
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• pp.142-163
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• 2020

조선 전기 적개공신(1467, 1476 초상 제작)과 정국(1506)·정난공신(1507), 위사공신(1545) 초상까지를 아청색 흑단령 차림이 등장하여 정착하는 '관복 제2기' 공신 초상으로 규정하고 적개공신 손소, 오자치, 장말손 초상 3점, 그리고 정국·정난공신 유순정과 홍경주, 정국공신 이우와 유홍의 초상 4점, 총 7점을 대상으로 분석하였다. '예복 흑단령' 차림의 적개공신 초상의 사모는 성종 초 높아졌던 모체가 다시 낮아졌고 모정은 둥글게 표현되었다. 너비가 넓은 타원형의 사모 양각에 무늬는 표현되지 않았다. 아청색 단령과 답호, 철릭에도 무늬가 표현되지 않았다. 단답호와 철릭의 색상이 각각 녹색과 홍색으로 통일되었으며 단령과 답호의 무는 '안팎주름무'로, 신발은 백화로 표현되었다. 적개공신 초상은 책록 시의 품계가 아닌, 초상 제작 시의 품계로 그려졌음을 보여 주었다는 점에서 주목할 만하다. '시복 흑단령' 차림의 정국·정난공신 초상은 사모의 경우, 적개공신 초상에서 낮아진 사모 모체의 형태는 그대로 유지되었으나 모정 부분이 조금 좁아지고 앞으로 휜 형태로 변하였으며 사모 양각은 전체적으로 너비가 균일한 타원형으로 표현되었다. 그리고 전 시기와 마찬가지로 사모 양각에도 무늬가 표현되지 않았다. 당하관 초상의 복식에는 무늬가 표현되지 않았으나 당상관 초상의 복식에는 운문(雲紋) 등이 표현되기 시작하였다. 또 단령은 안감 없는 홑단령으로 표현되었다. 당상관 초상에서는 홍색 답호와 녹색 철릭을, 당하관 초상에서는 녹색 답호와 남색 철릭이 표현되었다. 따라서 당상·당하 신분에 따라 받침옷의 색상이 구별되었음을 알 수 있었다. 단령과 답호에는 '안팎주름무'가 그대로 유지되었다. 신발은 흑화로 표현되었으며 흉배와 품대는 공신 책록 당시의 품계가 반영되어 있었다. 한편 위사공신 초상은 확인할 수 없었으나 명종대의 기록화를 통해 중종대의 단령 제도와 유사함을 알 수 있었다. 이상으로 살펴 본 제2기 적개공신 공신 초상을 통해 흑단령 차림을 공신 초상의 관복으로 사용하기 시작하였다는 점, 공신 초상 제작 시기의 품계를 초상에 반영하였다는 점을 확인할 수 있었다. 정국공신 초상 이후 복식에 무늬가 표현되기 시작하였는데 특히 흑단령의 무늬 유무로 당상·당하를 구별할 수 있게 되었다. 그리고 표현된 관복 모습은 당시의 관복 규정이나 관행과 일치함을 확인할 수 있었다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제15권2호
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• pp.93-98
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• 1993

Pleomorphic adenoma is the most common of all salivary gland tumors, constituting over 50% of all cases of tumors of both major and minor salivary gland origin and approximately 90% of all benign salivary gland tumors. Of the major salivary glands, the parotid gland is the most common site of the pleomorphic adenoma. It may occur, however, in any of the major gland or in the widely distributed intraoral accessory salivary glands. The palatal glands are frequently the site of origin of tumors, and other parts of origins are as follows: upper and lower lip, buccal mucosa, tongue and occasionally other sites. The majority of the lesions are found in patients in the fourth to sixth decades, but they are also relatively common in young adults and have been known to occur in children. It is somewhat more frequent in women than men. The term "mixed tumor" has masquaeraded under a great variety of names throughout the years (e.g., enclavoma, branchioma, endothelioma, enchondroma), but the term "pleomorphic adenoma" suggested by Willis characterizes closely the unusual histologic pattern of the lesion. The accepted treatment for this tumor is surgical excision. The intraoral lesions can be treated somewhat more conservatively by extracapsular excision. In general, Lesions of the hard palate should be excised with the overlying mucosa, while those in lining mucosa, such as the lips, soft palate and buccal mucosa often can be treated successfully by enucleation or extracapsular excision. In our hospital, we experienced two patients who were identified pleomorphic adenoma which occurred at buccal mucosa, submandibular gland. The lesions were successfully treated by surgical excision.

• 한국의상디자인학회지
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• 제22권2호
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• pp.137-149
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• 2020

The purpose of this study is to identify the perception of New-Hanbok Skirts that female consumers have and to analyze the design preference based on the analysis of designs of skirt products currently in distribution. Based on the analysis of the New-Hanbok brand website, the research was conducted through a survey of 355 women in their 20s and older. The survey consisted of 24 questions which were divided into pattern, color, material, and recognition type questions. The research results are as follows. First, most adult women answered that a New-Hanbok skirt is appropriate to wear at a holiday, anniversary, or outer dress for the spring and autumn seasons, and the design is the most important thing when considering buying. However, the ratios of wearing experience and possession were very low. Second, the preferred design aspects of a New-Hanbok skirt were the Jarak-skirt or Deot-skirt, calf-length, 5cm width of the waistband, 3cm uniform crease, a normal length waistband, bright neutral colors, and a waistband in different colors. For the double skirt, the design that shows 4cm of lining fabric and the different colors of upper fabric was the most preferred. The most preferred material was plain cotton. On the other hand, small floral patterns were preferred for patterned materials, and opaque upper fabric was most preferred for the double skirt. Through this study, it was confirmed that the Hanbok is seen as a positive aspect for modern consumers.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제27권1호
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• pp.32-36
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• 2001

To investigate epithelial cell proliferation reactivity in the odontogenic cysts, the expression of c-erbB-2 oncoprotein by epithelial lining was studied in odontogenic keratocyst(OKC, n=10), dentigerous cyst(DC, n=12), radicular cyst(RC, n=12) and normal dental follicle(n=7). The c-erbB-2 immunoreactivity was studied using a streptavidine- biotin- peroxidase method with polyclonal rabbit antihuman antibody to c-erbB-2 oncoprotein which is known to react with formalin fixed, paraffin-embedded sections and the intensity of staining was determined by manually. In all of 10(100%) OKCs, showed positive expression for c-erbB-2 oncoprotein compared with 10/12(83.3%) in DCs, 11/12(91.7%) in RCs and 5/7(71.4%) in normal dental follicles. The expression within OKC was higher than that of DC, RC and dental follicle but statistically not significant(p>0.05) and but may reflects underlying genetic defect. These results demonstrate differences in c-erbB-2 expression between the epithelial linings of the three major odontogenic cyst types, indicating differences in proliferation activity and differentiation processes within these lesions. And, in particular, these results are able to explain the peculiar aggressive growth pattern of OKC.

• 대한치과보철학회지
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• 제25권1호
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• pp.227-242
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• 1987

This study was designed to investigate the pulpal effects of the glass ionomer cement. (Lining cement, G-C Co. Japan) For this purpose, 10 cats were selected, and Class V cavities were prepared on canines of the cats. One experimental group was filled with glass ionomer cement and the other group was filled with zinc phosphate cement . (G-C Co, Japan) The animals of the experimental and control group were sacrificed at 1,2,3,4,6, weeks after the experiment. For comparison of reparative dentin formation pattern in direction of the pulpal and fractured lateral surface, each of them was observed with scanning electron microscope. The findings led to the following conclusions; 1. Reparative dentin of the glass ionomer cement and zinc phosphate cement filling groups were formed on the internal surface of dentin as the shape of hemispherical and spherical with a rough surface. 2. Some of reparative dentin of the glass ionomer cement filling group was started to form at 1 week after experiment, and at 6 weeks after experiment, it had been increased gradually in number and size. 3. Reparative dentin of zinc phosphate cement filling group was formed vigorously, however, gradually was decreased in number and size, and disappeared at 6 weeks after experiment. 4. During the formation of reparative dentin, peritubular dentins were indistinguishable. 5. The diameter of dentinal tubules of reparative dentin has been decreased, during the reparative dentin formed, and it became very irregularly at 6 weeks after experiment.

• Asian Pacific Journal of Cancer Prevention
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• 제17권7호
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• pp.3411-3416
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• 2016

Background: Nasopharyngeal carcinoma (NPC), a malignancy arising from the epithelial lining of the nasopharynx, is distinct from others cancers in terms of its epidemiologic features. It is rare in most parts of the world except for a few regions with populations of Mongoloid origin. Objectives: To study the expression pattern of Epstein Barr virus (EBV) encoded oncoproteins EBNA1 and LMP1 in different histological types of NPC and to correlate expression patterns with sex, age and histological types. Materials and Methods: A total of 40 formalin-fixed, paraffin-embedded NPC biopsy samples and tissues from 20 healthy controls were collected to study the expression level of EBNA1 and LMP1 using immunohistochemistry. Results: EBNA1 and LMP1 expression was found in 92.5% and 90% respectively, of the cases and none of the control specimens. The expression patterns of EBNA1 and LMP1 were determined to be statistically significant (p<0.05) when correlated with sex, age and histological distributions. Also immunohistochemistry was found to be a sensitive technique in the detection of EBV. Conclusions: The study reveals that the potent oncoproteins EBNA1 and LMP1 were over expressed in our population cohort. Our findings are to some extent inconsistent with earlier reports as our population showed a higher expression of both EBNA1 and LMP1 compared to other studies.

• 보존과학회지
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• 제25권2호
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• pp.131-145
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• 2009

고령 양전동암각화는 녹색 사암으로 구성된 너비 6m 높이 3m의 암반의 절리면에 겹동그라미(3점)와 방패무늬(30점)의 그림들이 있다. 구성암석의 주 구성광물은 석영, 장석 및 녹니석이며, 교질물인 점토광물 중 녹니석이 대부분이나 일라이트와 소량의 방해석도 산출된다. 이들 점토광물들은 구성광물을 피복 및 성분을 교대하거나 공극을 충진하는 양상으로 산출된다. 수용성 염과 암석의 풍화현상과의 관계를 포함한 훼손양상과 암각화가 그려진 암반의 사면안정성을 조사하였다. 훼손양상은 주로 표면박리, 입자박락, 균열 및 파손 등이다. 그리고 절리와 균열이 발달한 암반은 낙석의 가능성이 있는 것으로 해석된다. 보존을 위해서는 균열 및 파손 부위에 대한 접착처리와 약해진 표면층에 강화처리가 필요하다.