• BMB Reports
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• 제38권5호
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• pp.555-562
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• 2005

Single nucleotide polymorphisms (SNPs) are becoming the most common type of markers used in genetic analysis. In the present report a SNP has been chosen to test the applicability of Real Time PCR to discriminate and quantify SNPs alleles on DNA pools. Amplification Refractory Mutation System (ARMS) and Mismatch Amplification Mutation Assay (MAMA) has been applied. Each assay has been pre-validated testing specificity and performances (linearity, PCR efficiency, interference limit, limit of detection, limit of quantification, precision and accuracy). Both the approaches achieve a precise and accurate estimation of the allele frequencies on pooled DNA samples in the range from 5% to 95% and don't require standard curves or calibrators. The lowest measurement that could be significantly distinguished from the background noise has been determined around the 1% for both the approaches, allowing to extend the range of quantifications from 1% to 99%. Furthermore applicability of Real Time PCR assays for general diagnostic purposes is discussed.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2002년도 학술대회지
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• pp.361-364
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• 2002

An electromagnetic flowmeter(EMF) was developed and its characteristics were compared with a commercial EMF. The measuring tube of the EMF was basically designed as 100 m diameter. A signal processing circuit was also developed for generating the magnetic field and converting the flow signal to flowrate and flow quantity. To obtain a more stable and reliable flow signal, the double magnetizing frequency was adopted for magnetizing the coil of the EMF For the characterization of EU, the uncertainty of calibrator was estimated within ${\pm}0.5{\%}$, the method of estimation was in accordance with the ISO 17025 recommendation. It was found that the flow signals between the electrodes were about ${\pm}60\;-\;{\pm}300{\mu}V$, which were sufficient for the discrimination of the flowmeter and protecting the noise. The test result against the calibrator, showed the good linearity in the range of $3 m^{3}/h\;and\;70m^{3}/h$. A commercialized design of the EMF will be technically more competitive in domestic and foreign market.

• 한국연초학회지
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• 제20권1호
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• pp.108-114
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• 1998

This study was conducted to evaluate the techniques or analyzing tobacco smoke by statistical treatment method for the analytical data through Asia Collaborative Study V. In addition to five smoke components analysis, consisting of TPM, water, nicotine, NFDPM, and puff count of four cigarettes samples, statistical parameters such as mean, standard deviation, box-and-whisker plots, h plots, k plots, regression coefficients, reproducibility (R), and repeatability (r) were also calculated. Analysis of water content of cigarette smoke was the most difficult task, whereas puff count analysis was the easiest as well recognized by all laboratories. Analysis of nicotine and puff count accounted for both the lowest and the highest variation among four parameters. The water coefficients indicated more randomness or variation in the slops. The NFDPM data exhibited both types of deviations from linearity. Water content of sample D indicated the highest difference between two single results and between two interlaboratory test results. As a whole, KGTRI ranked higher in the analytical techniques for statistical evaluation of results when compared with the practices of 28 other laboratories.

• 천문학회보
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• 제35권2호
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• pp.31.2-31.2
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• 2010

We have performed a spectroscopic study of globular cluster (GC) system associated with the Virgo cD galaxy M87 using the Subaru/FOCAS MOS mode. We derive ages, metallicities and abundance ratios from the GC spectra using Simple Stellar Population (SSP) models. The metallicity distribution function (MDF) obtained empirically based on Milky Way GCs is consistent with the MDF derived from SSP models. A comparison with a meta-analysis using literature data sample of 15 other GC systems shows good agreement with our results. The properties of GCs acquired from the spectra will be used to test the recent theoretical prediction of a significant inflection along the colour-metallicity relations (Yoon et al. 2006). If confirmed, the non-linearity of the relations would shed new light on the interpretation of the GC colour bimodality. The robustness of our results is being tested against the choice of a SSP model, measurement errors and sample selection towards the goal of better understanding the formation history of GCs and host galaxy.

• Structural Engineering and Mechanics
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• 제26권6호
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• pp.725-739
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• 2007

In recent years there are many plate bending elements that emerged for solving both thin and thick plates. The main features of these elements are that they are based on mix formulation interpolation with discrete collocation constraints. These elements passed the patch test for mix formulation and performed well for linear analysis of thin and thick plates. In this paper a member of this family of elements, namely, the Discrete Reissner-Mindlin (DRM) is further extended and developed to analyze both thin and thick plates with geometric nonlinearity. The Von K$\acute{a}$rm$\acute{a}$n's large displacement plate theory based on Lagrangian coordinate system is used. The Hu-Washizu variational principle is employed to formulate the stiffness matrix of the geometrically Nonlinear Discrete Reissner-Mindlin (NDRM). An iterative-incremental procedure is implemented to solve the nonlinear equations. The element is then tested for plates with simply supported and clamped edges under uniformly distributed transverse loads. The results obtained using the geometrically NDRM element is then compared with the results of available analytical solutions. It has been observed that the NDRM results agreed well with the analytical solutions results. Therefore, it is concluded that the NDRM element is both reliable and efficient in analyzing thin and thick plates with geometric non-linearity.

• 대한기계학회논문집B
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• 제30권9호
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• pp.834-841
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• 2006

In the present wort the Laser-induced Fluorescence (LIF) technique and Confocal Laser Scanning Microscopy (CLSM) have been combined to measure the temperature distribution across a micro-scale liquid layer as a direct and non-invasive method. Only the fluorescent light emitted from a very thin volume around a focal plane can be selectively detected, and it enables us to measure the liquid temperatures even at the close vicinity of the walls. As an experimental verification, a test section consists of two flat plates (for heating and cooling, respectively) separated by about 240 microns was made, and the methanol mixed with a temperature-sensitive dye, Rhodamine B, was filled in the gap between them. The measured temperature distribution across the gap showed good linearity, which is a typical characteristic of conduction heat transfer through a thin liquid layer. In result, the CLSM-LIF technique proposed in the present study was found to be a promising method to measure the local temperatures in the liquid flow field in microfluidic devices.

• 생약학회지
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• 제46권4호
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• pp.352-364
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• 2015

The aim of this study was to quantitatively analyze for quality assessment of eighteen marker compounds, including homogentisic acid, 3,4-dihydroxybenzaldehyde, spinosin, liquiritin, hesperidin, ginsenoside Rg1, liquiritigenin, ginsenoside Rb1, glycyrrhizin, 6-gingerol, atractylenolide III, honokiol, costunolide, dehydrocostuslactone, atractylenolide II, nootkatone, magnolol, and atractylenolide I, in Hyangsayukgunja-tang using an ultra-performance liquid chromatography-electrospray ionization-mass spectrometer. The column for separation of eighteen marker components were used a UPLC BEH $C_{18}$ analytical column ($2.1{\times}100mm$, $1.7{\mu}$) and kept at $45^{\circ}C$ by gradient elution with 0.1% (v/v) formic acid in water and acetonitrile as mobile phase. The flow rate and injection volume were 0.3 mL/min and $2.0{\mu}l$, respectively. The correlation coefficient of all marker compounds was ${\geq}0.9914$, which means good linearity, within the test ranges. The limits of detection and quantification values of the all analytes were in the ranges 0.04-1.11 and 0.13-3.33 ng/mL, respectively. As a result, five compounds, homogentisic acid, 3,4-dihydroxybenzaldehyde, spinosin, liquiritigenin, and atractylenolide I, in this sample were not detected and the amounts of the 13 compounds except for the 5 compounds were $8.10-6736.37{\mu}g/g$ in Hyangsayukgunja-tang extract.

• 한국항해항만학회지
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• 제30권8호
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• pp.631-636
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• 2006

본 연구에서는 자유수면 위를 고속으로 항주하는 트랜섬 선미를 채택한 선박에 의하여 발생되는 선박주위 및 트랜섬 선미 끝단에서의 비선형 자유수면 경계조건식을 만족하는 자유 수면에서의 파형을 해석하는 수치해석 기법에 대하여 연구, 선체주위 유동해석프로그램을 개발하였다. 개발된 수치해석 프로그램의 타당성을 검증하기 위하여 고속선인 Athena선형, KCS(KRISO container ship)선형에 대하여 수치해석을 실시하였으며, 그 결과를 모형시험에 의하여 계측된 실험결과와 서로 비교하였다.

• Journal of Radiation Protection and Research
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• 제37권2호
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• pp.70-74
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• 2012

Silicon carbide (SiC) is a promising material for neutron detection at harsh environments because of its capability to withstand strong radiation fields and high temperatures. Two PIN-type SiC semiconductor neutron detectors, which can be used for nuclear power plant (NPP) applications, such as in-core reactor neutron flux monitoring and measurement, were designed and fabricated. As a preliminary test, MCNPX simulations were performed to estimate reaction probabilities with respect to neutron energies. In the experiment, I-V curves were measured to confirm the diode characteristic of the detectors, and pulse height spectra were measured for neutron responses by using a $^{252}Cf$ neutron source at KRISS (Korea Research Institute of Standards and Science), and a Tandem accelerator at KIGAM (Korea Institute of Geoscience and Mineral Resources). The neutron counts of the detector were linearly increased as the incident neutron flux got larger.

• Bulletin of the Korean Chemical Society
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• 제35권1호
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• pp.211-217
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• 2014

A new needle-based device for headspace wire coated in-needle microextraction (HS-WC-INME) was fabricated using a nichrome wire coated with poly(ethylene glycol) (PEG) and poly(dimethylsiloxane) (PDMS) mixture. The proposed needle device was applied for the determination of volatile compounds in lavender and lavandin essential oils by gas chromatography. Fundamental parameters such as needle design, conditions of extraction and desorption were optimized along with the validation of the extraction and desorption efficiency. The optimal conditions were 30 min extraction at $50^{\circ}C$ and 2 min desorption at $240^{\circ}C$. The calibration curves showed good linearity with the suitable values of the coefficients of determination ($r^2$) greater than 0.99. The limits of detection for linalyl acetate, ${\beta}$-caryophyllene, linalool and (+)-limonene were 7.15, 9.04, 10.79 and 22.26 ng, respectively. Analytical recoveries were acceptable in the test samples, varying from 86.7% to 108.0%. The values of relative standard deviations for run to run showed range less than 0.9% while 3.9% through 7.2% for needle to needle. The proposed PEG-PDMS coating could be more suitable than PDMS coating to extract particular polar groups such as alcohols.