• Journal of Advanced Marine Engineering and Technology
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• v.21 no.3
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• pp.246-255
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• 1997

The wave washer springs are expected to behave non-linearly between forces and displace¬ments due to contractions of the height and due to expansions in radial direction. To find out the non -linearity of wave washer springs, the three dimensional plate analysis theory using the finite element method is adopted in this paper. The wave washer springs are considered to be three dimensional plate structures rather than frame structures, because their thickness is normally much smaller than their width. The displacements of nodal points due to small increment of force are calculated by the finite element method and the calculated nodal displacements are added to X - Y Z coordinates of nodal points. The new stiffness matrix of the system using the new coordinates of nodal points is adopted to calculate the another nodal displacements, that is, the step by step method is used in this paper. The relations between the increments of forces and displacements in each step are recorded and plotted in chart. The experimental results are compared with the calculated chart and it is shown that there are good coincidences between measured values and calculated ones.

• Mass Spectrometry Letters
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• v.4 no.2
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• pp.25-29
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• 2013

Proteomics for biomarker validation needs high throughput instrumentation to analyze huge set of clinical samples for quantitative and reproducible analysis at a minimum time without manual experimental errors. Sample preparation, a vital step in proteomics plays a major role in identification and quantification of proteins from biological samples. Tryptic digestion a major check point in sample preparation for mass spectrometry based proteomics needs to be more accurate with rapid processing time. The present study focuses on establishing a high throughput automated online system for proteolytic digestion and desalting of proteins from biological samples quantitatively and qualitatively in a reproducible manner. The present study compares online protein digestion and desalting of BSA with conventional off-line (in-solution) method and validated for real time sample for reproducibility. Proteins were identified using SEQUEST data base search engine and the data were quantified using IDEALQ software. The present study shows that the online system capable of handling high throughput samples in 96 well formats carries out protein digestion and peptide desalting efficiently in a reproducible and quantitative manner. Label free quantification showed clear increase of peptide quantities with increase in concentration with much linearity compared to off line method. Hence we would like to suggest that inclusion of this online system in proteomic pipeline will be effective in quantification of proteins in comparative proteomics were the quantification is really very crucial.

• Natural Product Sciences
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• v.21 no.3
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• pp.192-195
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• 2015

An antibacterial benzoquinone, 2,6-dimethoxy-1,4-benzoquinone, isolated from Ficus foveolata stems was used as a standard marker for establishment of quantitative HPLC analysis for the stem extracts of F. foveolata. The method utilized a TSK-gel ODS-80Ts column (5 μm, 4.6 × 250 mm) with the mixture of methanol and 5% acetic acid in water (24:76, v/v) as the mobile phase at a flow rate of 1 mL/min, and quantitative detection at 289 nm. The parameters i.e. linearity, intraday and interday precision, accuracy, specificity and sensitivity of the method were evaluated for method validation. The recoveries of the method were 99.5 - 103.6% and good linearity (R² ≥ 0.9999) was obtained. A high degree of specificity, sensitivity as well as repeatability and reproducibility (RSD less than 2 and 5%, respectively) were also achieved. Chloroform was served as the most suitable solvent for extraction of 2,6-dimethoxy-1,4-benzoquinone. The optimised sample preparation and HPLC method can be practically used in the routine quality control process of F. foveolata stem extracts.

• The Transactions of the Korean Institute of Electrical Engineers B
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• v.48 no.1
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• pp.19-26
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• 1999

A moving-coil-type linear motor, designed and fabricated, is consisted of the NdFeB permanent magnets with high specific energy as the stator, a coil-wrapped nonmagnetic hollow rectangular structure and an iron core as a pathway for magnetic flux. The interaction between permanent magmet and armature fie1d, so called "push/pull effect", is to shift the airgap flux density variation due to the magnet alone by a certain amount. Thrust therefore is shift downward or upward. The push/pull effect was presented through the FEM analysis and the static tests. We compared the thrust obtained through the FEM analysis with the static tests. Finally, we present the linearity and correction coefficients of the thrust in consideration of the push/pull effects.l effects.

• The Journal of the Korea institute of electronic communication sciences
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• v.12 no.2
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• pp.267-272
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• 2017

In this paper, we suggested that a RF-front module of down-converter that represents the lowest noise figure to receive high quality video signals because the attenuation occurs in the atmosphere over 20GHz. By budget analysis of CDR, SFDR and CIP3 of RF-FEM, we also analyzed the parameters and linearity that presents high dynamic range. The total gain of designed Ka-band down-converter is 61.8dBand noise figure is 1.05dB, so gain and noise figures show excellent properties. In the future, the designed RF-FEM will be applied to the Ka-band satellite down-converter for UHD-class video transmission.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.60 no.3
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• pp.524-530
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• 2011

This paper analyzes fault tolerance under a stator turn fault, according to the winding configuration. Improvement of torque characteristics and fault tolerance can be achieved by winding configuration without additional methods. And, torque characteristics and fault tolerance according to the winding configuration can be usually analyzed by analytical method. But, when the stator turn fault generates, compare to the steady-state, analysis of torque characteristics and fault tolerance using the analytical method is not accurate because it does not reflect influence in mutual inductance and magnetic non-linearity. Therefore, analysis of torque characteristics and fault tolerance has to be performed by using the numerical method under fault condition. This paper develops fault characteristics according to the winding configuration using the FEM-base model considered magnetic non-linearity. And, this paper suggests fault tolerance improvement according to the winding configuration, by the comparison of 8/12 and 10/12 models, under fault condition.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.102-102
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• 2019

This study developed a HPLC analysis method for the determination of avicularin (AL) and quercitrin (QT) in Astilbe chinensis by HPLC. HPLC was performed on a Capcell Pak C18 MGII column ($4.6{\times}250mm$, $5{\mu}m$) with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and acetonitrile at a flow rate of 1.0 mL/min at $30^{\circ}C$ and a UV detection wavelength of 254 nm. The analytical method was validated for its specificity, precision, accuracy, and linearity. The limits of detection and quantitation were AL 0.09 and 0.28 mg/mL, QT 0.03 and 0.09 mg/mL respectively. Calibration curves showed good linearity (AL r2 > 0.99990 and QT r2 > 0.99994), and the precision of analysis was satisfied (less than AL 0.59%, QT 0.63%). Recoveries of quantified compounds ranged from AL 100.57 to 102.08%, QT 99.94 to 100.64%. These results indicate that the developed HPLC method was applied successfully to the detection of AL and QT in A. chinensis.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.100-100
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• 2019

This study attempted to establish a High Performance Liquid Chromatography (HPLC) analysis method for the determination of (-)-epicatechin gallate as a part of the quality control for the development of functional cosmetic materials from Penthorum chinense Pursh extracts. HPLC was performed on a Unison US-C18 column ($4.6{\times}250mm$, $5{\mu}m$) with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and methyl alcohol at a flow rate of 1.0 mL/min at $30^{\circ}C$. The analyte was detected at 280 nm. The HPLC method was performed in accordance with the International Conference on Harmonization (ICH) guideline (version 4, 2005) of analytical procedures with respect to specificity, precision, accuracy, and linearity. The limits of detection and quantitation were 0.11 and 0.33 mg/mL, respectively. Calibration curves showed good linearity (r2 > 0.9999), and the precision of analysis was satisfied (less than 0.6%). Recoveries of quantified compounds ranged from 99.51 to 101.92%. This result indicates that the established HPLC method is very useful for the determination of marker compound in P. chinense Pursh extracts.

• Korean Journal of Pharmacognosy
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• v.50 no.2
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• pp.133-140
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• 2019

In our previous study, we found uracil, adenosine, protocatechuic acid, syringin (eleutheroside B) and scoparone (6, 7-dimethoxycoumarin) in the Oplopanax elatus Nakai Stem. High-performance liquid chromatography (HPLC) -UV was used to quality and quantify the internal marker compounds in the O. elatus extract after validation of method with linearity, limit of detection (LOD), limit of quantitation (LOQ), accuracy and precision. The specificity assessment visually confirmed that the substance was detected without the introduction of other substances. The established method showed high linearity of the calibration curve and coefficient of correlation ($R^2$) of over the 0.999. HPLC was reported as five standard compounds equivalent using the following linear equation based on the calibration curve. The accuracy of measurement was 84.34 ~ 119.74% and the relative standard deviation (RSD) value was 0.28 ~ 1.60%. In addition, our established method showed high repeatability. The RSD value was 1.10 ~ 6.81%. So, we found the amount of the internal marker compounds in the O. elatus extract. These results demonstrated that can be used to quality evaluation of the O. elatus.

• Journal of the Earthquake Engineering Society of Korea
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• v.25 no.1
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• pp.11-20
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• 2021

The seismic deformation method is conventionally used as a seismic design for a multi-utility tunnel in Korea. In the seismic deformation method, the soil ground's natural period is one of the most critical factors for calculating the ground displacement using cosine functions. Correction factors for the natural period and shear wave velocity have been used to consider the non-linearity of dynamic soil properties. However, the correction factors have been issued because the correction factors have not been sufficiently studied to consider Korea's regional conditions. This paper aims to evaluate the natural periods for the seismic deformation method considering Korea's ground conditions. Ground response analysis was performed using seven real earthquake records on twelve sites with different soil conditions where actual multi-utility tunnels are installed. As a result, natural periods of the sites were analyzed and new correction factors were proposed according to seismic performance and Korea's regional conditions.