• Analytical Science and Technology
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• v.32 no.3
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• pp.88-95
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• 2019

This study was conducted to establish the analytical method for the determination of cyanide in blood, urine, lung and skin tissues in rats. In order to detect or quantify the sodium cyanide in above biological matrixes, it was derivatized to Pentafluorobenzyl cyanide (PFB-CN) using pentafluorobenzyl bromide (PFB-Br) and then reaction substance was analyzed using gas chromatography mass spectrometer (GC/MS)-SIM (selected ion monitoring) mode. The analytical method for cyanide determination was validated with respect to parameters such as selectivity, system suitability, linearity, accuracy and precision. No interference peak was observed for the determination of cyanide in blank samples, zero samples and lower limit of quantification (LLOQ) samples. The lowest limit detection (LOD) for cyanide was $10{\mu}M$. The linear dynamic range was from 10 to $200{\mu}M$ for cyanide with correlation coefficients higher than 0.99. For quality control samples at four different concentrations including LLOQ that were analyzed in quintuplicate, on six separate occasions, the accuracy and precision range from -14.1 % to 14.5% and 2.7 % to 18.3 %, respectively. The GC/MS-based method of analysis established in this study could be applied to the toxicokinetic study of cyanide on biological matrix substrates such as blood, urine, lung and skin tissues.

• Journal of Microbiology and Biotechnology
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• v.34 no.6
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• pp.1322-1327
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• 2024

The accurate and rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) holds significant clinical importance. This work presents a new method for detecting methicillin-resistant Staphylococcus aureus (S. aureus) in clinical samples. The method uses an aptamer-based colorimetric assay that combines a recognizing probe to identify the target and split DNAzyme to amplify the signal, resulting in a highly sensitive and direct analysis of methicillin-resistance. The identification of the PBP2a protein on the membrane of S. aureus in clinical samples leads to the allosterism of the recognizing probe, and thus provides a template for the proximity ligation of split DNAzyme. The proximity ligation of split DNAzyme forms an intact DNAzyme to identify the loop section in the L probe and generates a nicking site to release the loop sequence ("3" and "4" fragments). The "3" and "4" fragments forms an intact sequence to induce the catalytic hairpin assembly, exposing the G-rich section. The released the G-rich sequence of LR probe induces the formation of G-quadruplex-hemin DNAzyme as a colorimetric signal readout. The absorption intensity demonstrated a strong linear association with the logarithm of the S. aureus concentration across a wide range of 5 orders of magnitude dynamic range under the optimized experimental parameters. The limit of detection was calculated to be 23 CFU/ml and the method showed high selectivity for MRSA.

• Journal of Biomedical Engineering Research
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• v.28 no.6
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• pp.756-767
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• 2007

For the combination of phosphor screens having various thicknesses and a photodiode array manufactured by complementary metal-oxide-semiconductor (CMOS) process, we report the observation of image-quality degradation under the irradiation of 45-kVp spectrum x rays. The image quality was assessed in terms of dark pixel signal, dynamic range, modulation-transfer function (MTF), noise-power spectrum (NPS), and detective quantum efficiency (DQE). For the accumulation of the absorbed dose, the radiation-induced increase both in dark signal and noise resulted in the gradual reduction in dynamic range. While the MTF was only slightly affected by the total ionizing dose, the noise power in the case of $Min-R^{TM}$ screen, which is the thinnest one among the considered screens in this study, became larger as the total dose was increased. This is caused by incomplete correction of the dark current fixed-pattern noise. In addition, the increase tendency in NPS was independent of the spatial frequency. For the cascaded model analysis, the additional noise source is from direct absorption of x-ray photons. The change in NPS with respect to the total dose degrades the DQE. However, with carefully updated and applied correction, we can overcome the detrimental effects of increased dark current on NPS and DQE. This study gives an initial motivation that the periodic monitoring of the image-quality degradation is an important issue for the long-term and healthy use of digital x-ray imaging detectors.

• Earthquakes and Structures
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• v.4 no.5
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• pp.451-470
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• 2013

It generally accepted that most building structures shall exhibit a nonlinear response when subjected to medium-high intensity earthquakes. It is currently known, however, that this phenomenon is not properly modelled in the majority of cases, especially at the design stage, where only simple linear methods have effectively been used. Recently, as a result of the exponential progress of computational tools, nonlinear modelling and analysis have gradually been brought to a more promising level. A wide range of modelling alternatives developed over the years is hence at the designer's disposal for the seismic design and assessment of engineering structures. The objective of the study presented herein is to test some of these models in an existing structure, and observe their performance in nonlinear static and dynamic analyses. This evaluation is done by the use of two of a known range of advanced computer programs: SAP2000 and SeismoStruct. The different models will focus on the element flexural mechanism with both lumped and distributed plasticity element models. In order to appraise the reliability and feasibility of each alternative, the programs capabilities and the amount of labour and time required for modelling and performing the analyses are also discussed. The results obtained show the difficulties that may be met, not only in performing nonlinear analyses, but also on their dependency on both the chosen nonlinear structural models and the adopted computer programs. It is then suggested that these procedures should only be used by experienced designers, provided that they are aware of these difficulties and with a critical stance towards the result of the analyses.

• Journal of the Korean Institute of Intelligent Systems
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• v.27 no.2
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• pp.144-149
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• 2017

In this paper, we developed an intelligent and wideband RF-receiver module to represent a high dynamic range and good linearity characteristics up to 650MHz-2700MHz frequency band. and implemented an intelligent digital-RF- distorted signal measuring system for the RF distortion (intermodulation) signals. Broadband RF-receive module was designed to represent the optimized linear parameters of the receiver to meet the low noise and wide dynamic range. The designed intelligent digital-distortion(intermodulation) signal measument system measured by applying the 1MHz IF of third intermodulation signal of DUT and Measured data was recorded by program on the PC monitor with GUI interface. By variable up to 650MHz-2700MHz measured data showed up to -127.8dBc to -138dBc of the distortion (intermodulation) signal. And developed intelligent digital- distortion signal measurement system can be used to measure intermodulation distortion signal of wireless system widely.

• Mass Spectrometry Letters
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• v.8 no.1
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• pp.8-13
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• 2017

Analysis of quinidine for fish tissues using single drop microextraction (SDME) coupled with atmospheric pressure matrix assisted laser desorption/ionization mass spectrometry (AP-MALDI-MS) are reported. Optimization conditions; such as extraction solvent, extraction time, pH of the aqueous solution, salt additions (NaCl), stirring rate, matrix type and concentration are investigated. Linear dynamic range (${\mu}M$), limit of detection, relative recovery%, and enrichment factor are 0.08-9.2, 0.05, $94.8{\pm}3.1-98.5{\pm}3.3%$, $4.34{\pm}0.28-4.40{\pm}0.30$, respectively. SDME-AP-MALDI-MS shows good intraday and interday reproducibility.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2006.11a
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• pp.709-711
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• 2006

According as optical storage becomes high-density, numerical aperture increases. Therefore, the shift characteristic of moving parts in an actuator for optical pickup becomes a critical design factor because of decrease in the tilt margin. The tilt angle is maximized when the position of moving parts is in a diagonal direction within a moving range. This is determined by design of structure and magnetic circuit of an actuator. Previous analysis method only predicts linear characteristics of moving parts. However, the result of shift characteristics of the moving parts considering structural and magnetic circuit's nonlinearity following the every position simultaneously shows us more realistic result. Therefore, we present analysis method considering nonlinearity of moving parts' position through FEM package using coupled-field analysis. Then, we will suggest hereafter a design guide by comparing the above results with experimental ones.

• Journal of Korean Institute of Industrial Engineers
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• v.2 no.1
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• pp.85-92
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• 1976

In this paper, the employment planning model is developed which is a decision-making model for determining the optimum employment level with respect to varying net manpower requirement for each planing period such that total cost in a planning horizon is minimized. It is constructed as a nonlinear programming model and a dynamic programming model on the basis of studies in the areas of production smoothing and manpower scheduling. Costs for a planning period are categorized into regular wage cost, hiring cost, and overtime cost. The first is a linear function. The other two cost functions are of quadratic nature. The planning horizon of this planning model is intermediate range (five years) for which a fair planning accuracy can be guaranteed. The model considers learning period for each job class. It is simple and an optimum solution can be easily obtained by direct search techniques.

• Journal of the Korean Society of Safety
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• v.12 no.3
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• pp.23-29
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• 1997

In the control of crane system, the traversing time of the trolley must be reduced as much as possible and the swing must be stopped at the end point. To design the minimum time control system, Pontryagim maximum principle is applied. In order to implement the control algorithm, the dynamic equation is linearlized at an equilibrium point, so that the linear time invariant state equation can be obtained. The overall performance of the closed loop system is evaluated by means of computer simulations and practical experiments in a broad range of working conditions. The effectiveness is proved through the experimental results for the anti-sway control of the load and the position control of trolly. It is expected that the proposed system will make an important contribution to the industrial fields.

• The Journal of Korea Robotics Society
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• v.6 no.1
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• pp.34-41
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• 2011

This paper presents a frequency-response test performed on an antagonistic actuation system consisting of two Mckibben pneumatic artificial muscles and a pneumatic circuit. A linear model, capable of estimating the dynamic characteristics of the antagonistic system in the operating range of pneumatic artificial muscles, was optimally calculated based on frequency-response results and applied to a multiple simultaneous specification control scheme. Trajectory tracking results showed that the presented multiple simultaneous specification controller, built experimentally by three PD typed sample controllers, satisfied successfully all required control specifications; rising time, maximum overshoot, steady-state error.