• Reproductive and Developmental Biology
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• v.33 no.2
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• pp.71-76
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• 2009

Salivary lipocalin (SAL1) is a member of the lipocalin protein family that has a property to associate with many lipophilic molecules. The importance of SAL1 during pregnancy in pigs has been suggested by our previous study which has shown that SAL1 is expressed in the uterine endometrium in a cell type- and implantation stage-specific manner and secreted into the uterine lumen. However, function of SAL1 in the uterus during pregnancy in pigs is not known. To understand SAL1 function in the uterus during pregnancy, we generated recombinant porcine SAL1 protein in an insect cell line. Porcine SAL1 cDNA was cloned into a baculovirus expression vector using RT-PCR and total RNA from uterine endometrium on day 12 of pregnancy, and the expression vector was used to generate recombinant Bacmid containing the SAL1 gene. The recombinant Bacmid was then transfected Sf9 cell to produce recombinant baculovirus. By infecting Sf9 cell with recombinant baculovirus, we established a SAL1-expressing insect cell expression system. Immunoblot analysis confirmed SAL1 expression in the infected cells. Recombinant SAL1 produced by the Sf9 cell line will be useful for understanding physiological function of SAL1 during pregnancy in pigs.

• Journal of the Ergonomics Society of Korea
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• v.34 no.5
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• pp.519-529
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• 2015

Objective: This study aims to analyze the characteristics of occupational accidents and injuries of telecommunications line and cable workers by type of workplace and operational process of cabling service and to provide baseline data in establishing the preventive policies for occupational accidents and injuries. Background: In order to set up the preventive policies for occupational injuries and illness systematically, the accident analysis by industry should be preceded. To establish more effective policies, it should be done by occupation for persons who work in various kinds of occupation rather than by industry for persons who do in the same occupation. Method: In this study, the 176 occupational accidents and injuries were classified by type of workplace - indoor (inside building) and outdoor (at the top of utility pole, in a manhole, or in the fields) - and also done by operational process involved at the time of the accident. By analyzing the characteristics of occupational injuries and illness by type of workplace and operational process, respectively, this study can be helpful in establishing the preventative policies for occupational accidents and injuries. Results: The characteristics of occupational accidents and injuries by type of workplace showed that there were differences in terms of accident rate between indoor and outdoor on age of the injured, while not on employment-size and work experience of the injured. In addition, the characteristics on accident type, agency of accident, parts of body affected, and operational process between indoor and outdoor workplaces were statistically different each other. Conclusion and Application: The findings of occupational accidents' characteristics can be applied to the establishment of systematic preventative policies for occupational accidents of telecommunications line/equipment workers.

• Biomedical Science Letters
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• v.23 no.3
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• pp.238-250
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• 2017

Patient's primary tumor-derived tumor cell lines likely represent ideal tools for human tumor biology in vitro and in vivo. Here, we describe eight human gastric carcinoma cell lines derived from established tumors in vivo upon subcutaneous transplantation of primary gastric carcinoma specimens in BALB/c nude mice. These xenografted gastric tumor cell lines (GTX) displayed close similarity with primary gastric tumor tissues in their in vivo growth pattern and genomic alterations. GTX-085 cells were resistant to cisplatin, while GTX-087 was the most sensitive cell line. GTX-085 was the only cell line showing a metastatic potential. Epithelial cell adhesion molecule (EPCAM) expression was especially strong in all tissue samples, as well as in cell cultures. GTX-139, the largest tumor graft obtained after injection, displayed distinct expression of CD44v6, fibroblast growth factor receptor 2 (FGFR2), and prominin 1 (PROM1, also known as CD133). In summary, we established eight xenograft gastric cancer cell lines from gastric cancer patient tissues, with their histological and molecular features consistent with those of the primary tumors. The established GTX cell lines will enable future studies of their responses to various treatments for gastric cancer.

• BMB Reports
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• v.47 no.5
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• pp.274-279
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• 2014

Bortezomib has been known as the most promising anti-cancer drug for multiple myeloma (MM). However, recent studies reported that not all MM patients respond to bortezomib. To overcome such a stumbling-block, studies are needed to clarify the mechanisms of bortezomib resistance. In this study, we established a bortezomib-resistant cell line (U266/velR), and explored its biological characteristics. The U266/velR showed reduced sensitivity to bortezomib, and also showed cross-resistance to the chemically unrelated drug thalidomide. U266/velR cells had a higher proportion of CD138 negative subpopulation, known as stem-like feature, compared to parental U266 cells. U266/velR showed relatively less inhibitory effect of prosurvival NF-${\kappa}B$ signaling by bortezomib. Further analysis of RNA microarray identified genes related to ubiquitination that were differentially regulated in U266/velR. Moreover, the expression level of CD52 in U266 cells was associated with bortezomib response. Our findings provide the basis for developing therapeutic strategies in bortezomib-resistant relapsed and refractory MM patients.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.19 no.4
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• pp.1-12
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• 2020

In this study, the effect of the social distance maintenance and pedestrian route system was analyzed for Seoul Station, one of the multi use facilities according to the COVID-19 pandemic. For analysis, the Seoul Station pedestrian network was established through the survey of the number of passengers and CAD floor plan. A pedestrian that maintaining Social Distance was implemented using the Social Force Model. Based on this, scenario analysis was proceed. As a result, when the walking line system was installed the average walking speed decreased compared to the current situation. but the average density was analyzed that maintain the walking level of service (LOS)'C', this mean walking line system is effective, and the effect of the walking line system was proved. It can be used as a pedestrian simulation model.

• Convergence Security Journal
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• v.5 no.1
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• pp.19-27
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• 2005

This paper presents the content regarding electronic medical examination chart and data processing for efficient medical examination and fast treatment by realizing remote medical examination system of mutual conversation type among 3 parties(patient, doctor, pharmacist) on internet base, and establishment of database enabled system integration for efficient data processing in both on-line and off-line mode by interconnecting ASP and SQL on IIS 4.0 web server, consultation between patient and doctor, medical examination on off-line mode, transmission of prescription sheet to the pharmacist designated by patient, preparation of medicine, semieternal storage of medical examination data owing to storage and check of medical examination data, more accurate medical examination and prescription using this medical examination data by patient and doctor, and so on. And, data processing between doctor and pharmacist is differently performed based on class such as general member and charge member, and service access right pursuant to this is endowed, so that certification of each member must follow by all means.

• Journal of Distribution Science
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• v.8 no.2
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• pp.5-12
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• 2010

The allotment rate for railway transportation keeps an yearly 6% in Korea. However, the railway logistics will cause the opposite result according to the continuous investment and logistics rationalization. The study on railway logistics as well as inter-Korean railway that might highly contribute to the development of railway logistics is not enough at all. The purpose of this paper is to study the revitalization strategy for inter-Korean railway by forecasting the demand and the scale of railway logistics depot. The revitalization strategies for inter-Korean railway through railway logistics depot are as followings. First, it is necessary to strengthen the partnership with coal user in the logistics depot. Second, it is encouraged to provide the financial assistance that are needed in the maintenance of the decrepit North Korea's track as well as the establishment of Donghae northern line that is from Gangneung to Jejin. Third, the railway cost on long/short transportation and large sized shipper is needed to apply in a flexible way. Fourth, it is necessary to obtain the railway traffic right by involving the foreign mining development. Fifth, it is encouraged to constantly find the small sized shipper like cement company.

• Journal of the Korean Society of Hazard Mitigation
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• v.11 no.2
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• pp.9-13
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• 2011

The Performance-based fire protection design required to construct super high-rise building is the active measure for the evaluation of fire risks and the establishment of fire protection systems on the basis of engineering analysis, which is more efficient and proper than existing prescriptive-based design. This study applied time-line analysis of RSET is required safe egress time and ASET is available safe egress time with the fire and evacuation simulation to analyze. The result of this study showed the sprinkler system increased ASET and fire detection and alarm system reduced RSET efficiently. Reduced evacuation time influences to secure the life safety. Also it is essential to maintain the fire suppression system and fire detection & alarm system properly. Database of fire movement and evacuation action program are useful for the performance-based design.

• Journal of East Asia Management
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• v.3 no.2
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• pp.21-41
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• 2022

This study aims to analyze the characteristics of the management situation of the Korea Railroad Corporation(KORAIL) through the management innovation process of the KORAIL and to suggest its implications for military application. Despite stable demand, the railway passenger industry had the limitation of not being able to abolish deficit routes due to public service obligations. In addition, the launch of the Suseo High-Speed Line has introduced a competitive system, posing a threat to corporate management. KORAIL wanted to overcome this crisis by innovating its management through the utilization of big data, improvement of the freight business, decentralization of demand, the introduction of tourism railroads, and development of station influence areas. By utilizing big data, KORAIL was able to optimize the railway fare system while reducing fixed costs spent on railway maintenance. It also drastically reduced the station of cargo and created a base station to pursue economies of scale. On the other hand, the existing exclusive station system was abolished to solve the chronic saturation of the downtown area, and the railway demand was moved to Gwangmyeong Station and Suwon Station to optimize the passenger supply. In particular, it developed a new business model called the tourism railway by developing the mountain Byeokjin Line, which was a chronic deficit line, and sought to improve liquidity through the development of the station influence area. Such a process of innovation at KORAIL suggests an appropriate direction in seeking ways to innovate the military medical institutions. First of all, the necessity of improving organizational immersion through the development of a personnel structure suitable for the compulsory organization, while expanding the facilities of the division and corps, and reducing the time required for medical treatment and waiting through the establishment of a data-based medical system was suggested. Next, it was also discussed to integrate the National Health Medical College, which received accreditation as a medical facility through the designation of advanced general hospitals and is ultimately under discussion with the Medical Institution. Through this, we hope that the military medical institutions, which are facing various challenges, will overcome existing limitations and be re-lighted as innovative institution that provides comprehensive public health services.

• Strategy21
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• s.30
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• pp.63-98
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• 2012

Northeast Asia has a multi-layered security structure within which major economic and military powers both confront one another and cooperate at the same time. Major regional powers maintain mutually cooperative activities in the economic sphere while competing one another in order to secure a dominant position in the politico-military arena. The multifarious threats, posed by the North Korea's nuclear development, territorial disputes, and maritime demarcation line issues demonstrate that Northeast Asia suffers more from military conflicts and strifes than any other region in the world. Specifically, major maritime security threats include North Korea's nuclear proliferation and missile launching problems as well as military provocations nearby the Northern Limit Line(NLL) as witnessed in the Cheonan naval ship and Yeonpyong incidents. The ROK Navy has been supplementing its firm military readiness posture in consideration of North Korea's threats on the NLL. It has performed superb roles in defending the nation and establishing the Navy advanced and best picked. It also has been conducive to defend the nation from external military threats and invasion, secure the sea lanes of communications, and establish regional stability and world peace. In order to effectively cope with the strategic environment and future warfares, the ROK Navy needs to shift its military structure to one that is more information and technology intensive. In addition, it should consolidate the ROK-US alliance and extend military cooperative measures with neighboring countries in the Asia-Pacific region. Evolved steadily for the last 60 years, the ROK-US alliance format has contributed to peace and security on the Korean peninsula and in the Northeast Asian region. In conclusion, this manuscript contends that the ROK Navy should strive for the establishment of the following: (1) Construction of Jeju Naval Base; (2) Strategic Navy Equipped with War Deterrence Capabilities; (3) Korean-type of System of Systems; (4) Structure, Budget and Human Resources of the Naval Forces Similar to the Advanced Countries; and (5) Strategic Maritime Alliance and Alignment System as well as Domestic Governance Network for the Naval Families.