• Proceedings of the KSR Conference
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• 2008.11b
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• pp.776-781
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• 2008

In this paper, comments on the needs of establishment of railway test line - capable of mid and long term performance evaluation of vehicle/infra/electricity/signal component/system - are described. Based on the previous studies, national/social/economic needs, the states of similar lines of foreign countries, concept of test line, and basic application plan are summarized. Especially, core things needed to the establishment of test line are commented.

• Journal of Communications and Networks
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• v.11 no.1
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• pp.72-83
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• 2009

We have studied the effect of head of the line blocking (HOLB) on session initiation protocol (SIP) session establishment delays. Our results are based on experiments performed in a test bed and on the public Internet. We used the stream control transmission protocol (SCTP) as a transport for SIP because SCTP can be configured to suffer or to avoid HOLB. Our experiments show that the effect of HOLB on session establishment delays generally starts to be significant starting at fairly low packet loss rates. However, there are scenarios where network conditions are good enough to make the effect of HOLB insignificant.

• Journal of Embryo Transfer
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• v.19 no.3
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• pp.201-208
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• 2004

This study was performed to examine the effects of catalase and $\beta$-mercaptoethanol ($\beta$ME) on the establishment of clonal lines from porcine fetal fibroblast cells. Fibroblasts derived from a pig fetus (Day 50) were passaged two times before use. A single cell was seeded in 96-well plates and cultured in medium supplemented with or without catalase or $\beta$ ME. Cell colonies were passaged two times into 4-well dish. Cell lines with proliferating potential were classified as an established clonal cell line. In experience 1, the establishment efficiencies were examined by addition of catalase (100ng/$m\ell$) or $\beta$ME (100 uM) in culture medium. The establishment efficiency of $\beta$ME-added group (8.3％) was significantly higher than that of control group (3.2％, P<0.05). However, catalase did not have a positive efffct on the establishment efficiency. In experience 2, the establishment efficiencies were examined by addition of different concentrations of catalase (0-1,000 ng/$m\ell$) in culture medium. However, establishment efficiencies were not different among the different concentrations of catalase (0-2.6％). In experience 3. the establishment efficiencies were examined by addition of different concentrations of $\beta$ME(0-1,000 uM) in culture medium. The establishment efficiency was significantly higher in 100 uM $\beta$ME-added group (9.4％) compare to others (0-1.6％). The result of present study shows that the establishment efficiency of clonal cell lines can be enhanced by the culture in media supplemented with 100uM $\beta$ME. However, catalase did not have a positive effect on the establishment efficiency.

• Journal of Fashion Business
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• v.6 no.5
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• pp.125-135
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• 2002

The purpose of this study was to investigate the present state of e-business according to the establishment year and the sales approach of Dongdaemun clothing market and to present problems according to e-business activation of it and its developmental direction. The subjects for this study were selected out of Dongdaemun clothing markets being considered as more fashion-oriented markets and were divided into two categories both according to the establishment year and according to the sales approach. As a result, Dongdaemun clothing markets are mainly divided into traditional wholesale market, eastern wholesale & retail market, and western retail market and having dealings with each other according to the market's characteristics. The utility percentage of on-line e-business according to the establishment year and the sales approach showed that traditional wholesale market was 42%, eastern wholesale & retail market 0.75%, and western retail market 11%. This result indicated that the utility percentage of traditional wholesale market showed much higher than eastern wholesale & retail market and western retail market. This would be because the structural characteristics of clothing market delayed the utility of e-business. For the problems on the utility of on-line e-business through counselling, traditional wholesale market showed much higher than wholesale & retail market and retail market because it had little problems on design imitation in retail market due to export by order and mass production. The problems of low utility of on-line e-business in Dongdaemun clothing market would result from well-timed on-line update according to rapid goods cycle, constant satisfaction for additional order, standardization of size and quality, and inexpensive internet purchase despite of express expense.

• BMB Reports
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• v.44 no.8
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• pp.512-516
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• 2011

Establishment of immortalized human dermal papilla cells (DPCs) retaining the characteristics of DPCs would be a great help for hair researchers. We recently established a simian virus 40T (SV40T)-transformed human DP cell line (SV40TDPC). However, the cell line senesced around passage 25 and ceased proliferation. In this study, we introduced the human telomerase reverse transcriptase (hTERT) gene into SV40T-DPC and established an immortalized human DP cell line. The cell line, SV40T-hTERT-DPC, did not induce tumors when inoculated into nude mice. SV40T-hTERT-DPC maintained morphology of early passage DPCs, expressed markers of DPCs, and retained responses to Wnt/${\beta}$-catenin and bone morphogenic protein (BMP) signaling pathways known to be required for hair-inducing activity of DPCs. The data strongly suggest that SV40T-hTERT-DPC retains many characteristics of human DPCs in vivo without malignant transformation.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.06a
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• pp.1-16
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• 2002

• Journal of Korean Society for Geospatial Information Science
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• v.17 no.2
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• pp.123-131
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• 2009

The telecommunication technology in the 21st century has come up to the standard which could transmit various spatial information by means of on-line and the printing technology using computers has been on a par with presswork in quality. In addition, map users also would like to get various spatial information through on-line directly or indirectly. None the less, the supply system of national base map in our country has maintained the off-line sales method. It is owing to this reason that the nation's supply system has shown a high degree of inefficiency from the viewpoint of the inventory control, rapidity, and economical efficiency as compared with the on-line sales system in the developed countries. This study tried to analyze some problems of the present supply system in order to build up the foundation of the efficient map supply system and also developed the system such as On-demand maps, on-line paying and sales, inventory control, meta dat establishment, and security module, etc. This study tried to analyze some problems of the present supply system in order to build up the foundation of the efficient map supply system and also developed the system such as On-demand maps, on-line paying and sales, inventory control, meta dat establishment, and security module, etc.

• Journal of the Korean Institute of Telematics and Electronics A
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• v.30A no.2
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• pp.46-52
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• 1993

The estimation of yield for semiconductor devices requires not only establishment of critical area but also a new parameter of process defect density that contains inspection mean defect density related cleanness of manufacure process line, minimum feature size and the total number of mask process. We estimate the repaired yield of memory devide, leads the semiconductor technique, repaired by redundancy scheme in relation with defect density distribution function, and we confirm the repaired yield for different devices as this model. This shows the possibility of the yield estimation as statistical analysis for the condition of device related cleanness of manufacture process line, design and manufacture process.

• Journal of the Korean Society of Safety
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• v.11 no.2
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• pp.128-135
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• 1996

The aim of this study is that HPMA(Hazard Potentials in My Areas) System is to present for establishment of the voluntary safety management on industrial region. HPMA System Itself means that manager, supuryisor and worker on process-line find out the hazardous and/or unsafe potentials from their work places and make the plan of improvement by means of mutual communication with top manager and the department of safety staff, eventually put in the practice. In case that it takes the longer time to improve hazard potentials and is impossible improvement by existing technique, appropriate check-list will be used by the worker himself on the identified production line. Form this system, therefore, it makes them to get more and more sensitivity to the hazards. In this study, we present the voluntary safety management system which is controlable systematically and in the final analysis the direction of safety management by computerization of HPMA System which is activated in their work places.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.4
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• pp.492-499
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• 2009

A Silkie Bantam embryo fibroblast line (named SBF59 line) was successfully established by using direct explant culture and cryopreservation techniques. Cell morphology, viability, dynamic growth and contamination were tested and the karyotype and levels of isoenzymes of lactic dehydrogenase and malic dehydrogenase were analyzed. Four kinds of fluorescent protein extrogenes, including $pEGFP-N_3$, $pECFP-N_1$, $pEYFP-N_1$ and $pDsRed1-N_1$ were transfected into the cells. The results showed that the cells were healthy and possessed a fibrous structure without a change in morphology. The average viability of the cells was 96% before freezing and 90.5% after thawing. The growth curve appeared as typical "S" shape and the cell growth passed through a detention phase, a logarithmic phase and a platform phase; the estimated population doubling time (PDT) was 38.5 h; assays for the presence of bacteria, fungi, viruses and mycoplasmas were negative; the cell line showed no cross contamination when assessed by isoenzyme analysis; the chromosome number was 2n = 78 on more than 88% of occasions; four kinds of fluorescent protein extro-genes appeared to be expressed effectively with a high transfection efficiency between 18.3% and 42.3%. The cell line met the required quality control standard. It not only preserves the genetic resources of the important Silkie Bantam at the cellular level but also provides valuable materials for genomic, post-genomic, somatic cell cloning research and other applications.