• Journal of Microbiology and Biotechnology
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• 제14권2호
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• pp.225-230
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• 2004

A plasminogen kringle domain 1 to 3, rKl-3, was expressed in Escherichia coli under the control of T7 promoter. For the cost-effective production of rKl-3, the induction process was analyzed and optimized. Induction characteristics with lactose were analyzed in terms of induction time and inducer concentration in various culture conditions including batch and high-cell-density fed-batch cultures. In the fed-batch culture, the induction around 6 h after initiation of the DO-stat fed-batch culture resulted in the highest expression level of rKI-3 among the induction points examined. The highest demand of oxygen at this point was crucial for the maximum expression level of rKI-3. As the lactose concentration increased, the expression level also increased, though the expression level showed a plateau above a concentration of 14 mM of lactose. Lactose acted less specifically than IPTG since most of it was hydrolyzed to glucose and galactose. However, using lactose, the cell growth and the maximum expression level of rKl-3 increased by 20% and 24%, respectively, compared with those using IPTG in the fed-batch culture. The lactose seemed to be hydrolyzed by intracellular and extracellular $\beta$-galactosidase liberated by cell lysis at the same time. Residual concentration of glucose was maintained to a a limit of detection by high performance liquid chromatography, and galactose was not consumed by the host strain Escherichia coli BL2l(DE3).

• 한국농공학회지
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• 제30권1호
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• pp.38-49
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• 1988

This paper presented as follows results of laboratory model tests with various shaped footings on soil bed reinforced with the strips on the base of behaviour of soil structure according to the loads and triaxial test results reinforced with geotextiles. Their parameters studied were the effects on the bearing capacity of a footing of the first layer of reinforcement, horizontal and vertical spacing of layers, number of layers, tensile strength of reinforcement and iclination load to the vertical 1.Depending on the strip arrangement, ultimate bearing capacity values could be more improved than urreinforced soil and the failure of soil was that the soil structure was transfered from the macrospace to microspase and its arrangement, from edge to edge to face to face. 2.The reinforcement was produced the reinforcing effects due to controlling the value of factor of one and permeable reinforcement was never a barrier of drainage condition. 3.Strength ratio was decreased as a linear shape according to increment of saturation degree of soil used even though at the lower strength ratio, the value of M-factor was rot influenced on the strength ratio but impermeable reinforcement decreased the strength of bearing capacity. 4.Ultimate bearing capacity under the plane-strain condition was appeared a little larger than triaxial or the other theoretical formulars and the circular footing more effective. 5.The maximum reinforcing effects were obtained at U I B=o.5, B / B=3 and N=3, when over that limit only acting as a anchor, and same strength of fabric appeared larger reinforcing effects compared to the thinner one. 6.As the LDR increased, more and more BCR occurred and there was appeared a block action below Z / B=O.5, but over the value, decrement of BCR was shown linear relation, and no effects above one. 7.The coefficient of the inclination was shown of minimum at the three layers of fabrics, but the value of H / B related to the ultimate load was decreased as increment of inclination degree, even though over the value of 4.5 there wasn't expected to the reinforcing effects As a consequence of the effects on load inclination, the degree of inclination of 15 per cent was decreased the bearing capacity of 70 per cent but irnproved the effects of 45 per cent through the insertion of geotextile.

• 소성∙가공
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• 제17권2호
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• pp.124-129
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• 2008

Net shape forging technologies give many effects into the costs and qualities for the finished products. So, the studies to reduce the additional machining amount are very important in forging industry. Specially, there are two main topics in cold forging industry, such as, tool life and precision forging. In this study, new forging technique was proposed to eliminate the machining process for fixing up the length and improve the lead accuracy of gear. The luck-up hub is manufactured through many processes, such as upsetting, piercing and direct extrusion. The gear is formed in direct extrusion process; however, lead accuracy of the gear is over allowance limit. Therefore, the additional sizing process must be added. In this study, process design for closed-die forging of a lock-up hub used for a component of automobile transmission was made using three-dimensional finite element simulations, and the strain distributions and velocity distributions are investigated through the post processor. The rigid-plastic finite-element method for back pressure forging has been used in order to reduce development time and die cost. Using the FEM simulation, we found the optimum value of back pressure. The prototypes of lock-up hub parts were forged into the net-shape. In the experiment, lead precision of tooth are measured by the CCMM(Contact Coordinate Measuring Machine). The dimensional accuracy of forged part was improved up to the 40% when back press was applied.

• 한국산학기술학회논문지
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• 제10권6호
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• pp.1275-1280
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• 2009

최근 교량설계에서 외부 프리스트레스를 도입한 장지간 및 효율적인 단면활용 등과 같은 연구가 활발하게 진행되고 있다. 강구조물에 프리스트레스를 도입하면 큰 하중에서 탄성변형의 극한값이 증가하게 되어, 강재량을 줄일 수 있고, 비용의 측면에서 경제성이 있는 것으로 평가 되었다. 이러한 이유 때문에 우리나라에서도 최근 가설교량 시공시 프리스트레스를 도입하여 응력보정 및 처짐보정이 가능하고, 비교적 작은 단면으로 장지간 교량을 시공할 수 있어 통수단면을 충분히 할 수 있는 여러 가지 공법이 개발되고 있다. 따라서 본 논문에서는 가설 강교량에 프리스트레스 도입방법 및 고강도 텐던의 배치에 따른 영향, 강판과 고강도 텐던의 복합구조에 대한 효과를 기존 가설공법과 비교하여 검증 하고자 한다.

• 콘크리트학회논문집
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• 제17권6호
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• pp.939-946
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• 2005

비균질 재료인 콘크리트의 강성 특성과 성능저하 현상을 웨이블릿 변환을 이용한 다중해상도해석을 통해 각 관찰 규모에 따라 동질화 과정의 적용성 및 거시적 손상지수의 평가 등을 연구하였다. 연속적인 Haar 웨이블릿 변환은 기존 강성행렬의 특성을 연속적인 축소규모로의 복제를 통해 미세규모로부터 거시규모로의 축소 또는 복원 과정을 나타내었고 이는 선형구조계의 크기별 스펙트럼 특성의 보존, 즉 타원성, 철면성 그리고 양의 정부호성을 보존하여 각 규모별 해의 유효성을 확인하였다. 웨이블릿 계수를 이용한 기존 강성의 평균은 거시단계의 변형에너지와 상호관계를 가지고 아래 단계로의 축소, 윗 단계로의 복원을 자유롭게 할 수 있는 장점이 있다. 이러한 다중해상도해석의 예제로서 1차원 및 2차원 2상복합체를 가지고 유한요소해석을 통해 기존 이론의 검증과 최소고유치의 각 크기단계별 변화 과정, 원 축소 구조계의 해의 유일성 그리고 국부적 손상지수의 동질화 여부 등을 검사하였다. 이러한 동질화 축소 과정은 자유도가 큰 비선형 구조계로의 적용의 첫 단계를 제공하였다.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2008년도 춘계 학술발표회 제20권1호
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• pp.261-264
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• 2008

횡방향으로 구속된 콘크리트의 응력-변형률 거동은 구속되지 않은 콘크리트와는 다른 거동을 한다. 보통강도 콘크리트에서 구속효과를 고려한 콘크리트 재료모델로는 Mander 모델이 대표적이며 고강도 콘크리트의 구속효과의 경우 여러 연구자들에 의하여 제안된 모델 중 공시체 수준의 실험결과와 잘 일치하는 Sakino-Sun 모델을 사용하였다. 보통강도에서는 Mander모델을 고강도 콘크리트에서는 Sakino-Sun 모델을 사용하였으나 중간 강도인 30-40MPa의 강도에서 Mander 모델과 Sakino-Sun 모델의 적용시 실험결과와 해석결과가 다소 차이를 보이며 또한 두 모델은 적용할 수 있는 최대 또는 최소 콘크리트 압축강도의 한계범위가 명확하지 않다. 따라서 이 연구에서는 30-40MPa의 강도의 횡방향으로 구속된 콘크리트의 비선형 재료모델을 제안하였다.

• 한국건축시공학회지
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• 제16권4호
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• pp.297-303
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• 2016

콘크리트 건조수축 균열을 제어하기 위하여 수축저감제(SRA)가 개발되었다. SRA는 콘크리트 미세공극의 표면장력을 작게 하여 수축량을 감소시키며, 콘크리트의 품질향상을 위하여 SRA의 사용이 증가되고 있다. 하지만 건조수축을 예측하기 위한 다양한 모델이 존재함에도 불구하고, SRA의 영향을 고려할 수 있는 예측방법이 아직까지 없는 실정이다. 따라서 SRA 콘크리트의 건조수축에 의해 발생하는 인장응력을 정확히 예측할 수 없고, 콘크리트 구조물의 정량적인 사용성 한계의 검토가 불가능하다. 본 연구에서는 SRA 콘크리트의 정량적인 건조수축 변형률 예측가능성을 제시하기 위하여, 건조수축실험값과 기존 모델에 의한 예측값을 비교하였다. 기존 모델에는 SRA의 영향을 고려할 수 없으므로, 실험결과에 근거하여 SRA 첨가율에 따른 수축저감계수를 도출하였고 기존 모델에 수축저감계수를 적용하여 예측값을 구하였다. 그 결과 AIJ 모델, ACI 모델, GL2000 모델은 ${\pm}10%$의 오차범위 내에서 예측값과 실측값이 전반적으로 양호한 상관관계를 보였지만, CEB-FIP 모델과 B3 모델은 예측값이 실측값을 과소평가 하는 것으로 나타났다.

• Geomechanics and Engineering
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• 제8권2호
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• pp.187-220
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• 2015

Soil disturbance induced by installation of mandrel driven vertical drains decreases the in situ horizontal hydraulic conductivity of the soil in the vicinity of the drains, decelerating the consolidation rate. According to available literature, several different profiles for the hydraulic conductivity variation with the radial distance from the vertical drain, influencing the excess pore water pressure dissipation rate, have been identified. In addition, it is well known that the visco-plastic properties of the soil also influence the excess pore water pressure dissipation rate and consequently the settlement rate. In this study, a numerical solution adopting an elastic visco-plastic model with nonlinear creep function incorporated in the consolidation equations has been developed to investigate the effects of disturbed zone properties on the time dependent behaviour of soft soil deposits improved with vertical drains and preloading. The employed elastic visco-plastic model is based on the framework of the modified Cam-Clay model capturing soil creep during excess pore water pressure dissipation. Besides, nonlinear variations of creep coefficient with stress and time and permeability variations during the consolidation process are considered. The predicted results have been compared with V$\ddot{a}$sby test fill measurements. According to the results, different variations of the hydraulic conductivity profile in the disturbed zone result in varying excess pore water pressure dissipation rate and consequently varying the effective vertical stresses in the soil profile. Thus, the creep coefficient and the creep strain limit are notably influenced resulting in significant changes in the predicted settlement rate.

• Computers and Concrete
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• 제9권4호
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• pp.311-325
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• 2012

In this paper, we examine the behavior of the High-Rate Brittle Microplane (HRBM) concrete model based on a series of penetration experiments. These experiments were conducted with three different slab thicknesses (127, 216 and 254 mm) that provided a significant challenge for the numerical simulations. The 127 mm slab provided little resistance, the 216 mm slab provided nominal resistance and the 254 mm slab approached the perforation limit thickness of the projectile. These experiments provide a good baseline for evaluating material models since they have been shown to be extremely challenging; in fact, we have not encountered many material models that can provide quantitatively predictive results in terms of both projectile exit velocity and material damage. In a companion paper, we described the HRBM material model and its fit to various quasi-static material property data for WES-5000 concrete. In this paper, we show that, when adequately fit to these quasi-static data, the HRBM model does not have significant predictive capabilities, even though the quasi-static material fit may be exceptional. This was attributed to the rate-dependent response of the material. After various rate effects were introduced into the HRBM model, the quantitative predictive nature of the calculations dramatically increased. Unfortunately, not much rate-dependent material property data are in the literature; hence, accurate incorporation of rate effects into material models is difficult. Nonetheless, it seems that rate effects may be critical in obtaining an accurate response for concrete during projectile perforation events.

• 약학회지
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• 제56권4호
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• pp.222-229
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• 2012

In order to determine acetylcholine and choline in the biological samples, the specific enzymes of acetylcholinesterase (AChE) and choline oxidase (ChO), which utilize acetylcholine and choline as substrates, were employed to convert substrates to $H_2O_2$. The produced $H_2O_2$ was coupled to 4-aminoantipyrine/phenol with peroxidase (PO) yielding quinoneimine dye which was measured at 508 nm. In the present enzymatic spectrophotometric analysis the product at the equilibrium state was measured considering accuracy, precision, time and cost of the analysis. The developed analytical method yielded good linearity (calibration curve; $A_{508}$=9534[acetylcholine]+0.009, correlation coefficient ($R^2$); 0.999) with detection limit of $1.11{\times}10^{-7}M$, reasonable precision (relative standard deviation; 0.10~1.62% at $2.5{\times}10^{-6}M{\sim}1.25{\times}10^{-4}M$) and accuracy (relative error; -0.24~0.97% at $4.13{\times}10^{-6}M{\sim}1.01{\times}10^{-4}M$) for acetylcholine chloride standard solution. The concentrations of acetylcholine and choline in human serum were found as $3.20{\times}10^{-5}M$ and $1.14{\times}10^{-4}M$, respectively. The brain tissues of Sprague-Dawley strain rat contained 9.82${\mu}g/g$ of acetylcholine and 6.53 ${\mu}g/g$ of choline in the cerebrum, while 7.37 ${\mu}g/g$ of acetylcholine and 5.34 ${\mu}g/g$ of choline in the cerebellum.