• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.149.1-149
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• 2003

A lily strain of Cucumber mosaic virus (LK-CMV) was not able to systemically infect zucchini squash (Cucurbita pepo), while Fny strain of CMV (Fny-CMV) caused systemic mosaic and stunting symptom at 4 days post-inoculation on the same host species. The pathogenicity of LK-CMV in zucchini squash was investigated by reassortments of genomic RNAs of LK-CMV and Fny-CMV for infection, as well as by pseudorecombinants generated from biologically active transcripts of CDNA clones of LK-CMV and Fny-CMV, respectively. The assessments of pathogenicity for LK-CMV indicated that RNA2 of LK-CMV was responsible for systemic infection in zucchini squash. In the protoplast of zucchini squash, the RNA accumulations of all constructed pseudorecombinants were indistinguishable and LK-CMV replication was slightly lower than that of Fny-CMV, suggesting that the inability of LK-CMV to infect squash plants was responsible for the poor efficiency of virus movement, rather than the reduction of replication function.

• 한국자원식물학회지
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• 제32권6호
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• pp.730-734
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• 2019

Cryopreservation is one of the ideal and suitable methods for long-term storage of plant germplasm. The plant contaminated with diseases and pathogens are decreased the multiplication rate, survival rate and high quality of plants after cryopreservation. The aim of this work was to improve a micropropagation method for lily in Korea, which is indigenous plant. In the last process of rinsing scales after surface-sterilization, we tried to control the diseases and pathogens lived within the tissue by rinsing in 0.03% sodium hypochlorite (NaClO) instead of sterile distilled water. Bulb scales of Lilium were cultured in vitro on MS medium supplemented with Plant Preservative Mixture (PPM). The results showed that L. tsingtauense accessions were observed ranged from 53.9 to 100% with a mean value of 76.8% and L. hansonii accessions were checked from 84.5 to 85.5% with a mean of 85% survival rate. The newly small bulb formed from bulb-scales was transferred to MS medium. We checked the presence of microorganisms and survival rate after 3 weeks in culture after examination of bacterial incidences. The results indicated that the non-contamination rate were shown ranged from 75.0 to 94.1% with mean value of 83.2% in L. tsingtauense species, and that L. hansonii were observed 85.1 to 91.7% with mean value of 88.4%. This study will provide a valuable basis for establishment of effective axenic cultures for in vitro micropropagation of Korean native lily species.

• The Plant Pathology Journal
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• 제18권2호
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• pp.85-92
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• 2002

A new isolate of Cucumber mosaic virus (CMV), identified as Li-CMV was isolated from a diseased Korean native lily (Lithium tsingtauense Gilg). Biological and serological properties of Li-CMV were characterized, and reverse transcription-polymerase chain reaction (RT-PCR) analysis, restriction enzyme profiling of RT-PCR products, and nucleotide sequence analysis of RNA3 of the virus were performed in this study. Remarkable differences in symptoms between Li-CMV and ordinary CMV strains were found in tobacco plants and Datura stramonium. Li-CMV-infected tobacco plants (cv. Xanthi-nc and cv. Samsun) induced chlorotic ringspots on uninoculated upper leaves, and the symptom expression was delayed or faint whereas, ordinary CMV strains induced green mosaic symptoms on the plant. Systemic infections were observed on Nicotiana benthamiana with severe mosaic symptom. Restriction mapping analysis of RT-PCR products using MspI showed that Li-CMV belonged to CMV subgroup I. A full-length CDNA copy of RNA3 for the virus was amplified by RT-PCR, cloned, and its complete nucleotide sequence was determined. The RNA3 of Li-CMV was 2, 232 nucleotides long, and consisted of two open reading frames of 843 and 657 bases encoding 3a protein (movement protein) and coat protein, respectively. Results of this study indicate that Li-CMV is a novel strain and belongs to subgroup I of CMV in the genus Cucumovirus.

• 한국식물병리학회지
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• 제14권5호
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• pp.452-457
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• 1998

Thirty-eight isolates of Phytophthora sp. caused rots on roots and basal stems were collected from five flowering plants from 1992 to 1997 at eight cultivation areas in Korea. All the isolates were identified as P. nicotianae based on following characteristics. The fungus produced markedly papillate, not caducous and ovoid to spherical sporangia, abundant chlamydospores, and small oospores with amphigynous antheridia only when paired with either A1 or A2 mating type. All isolates grew well at 35$^{\circ}C$ and showed distinct arachnoid colony patterns on CMA and PDA. Sizes of sporangia and chlamydospores of five representative isolates from each plant averaged 43-52$\times$30-38 ${\mu}{\textrm}{m}$ and 28 ~34 ${\mu}{\textrm}{m}$. Mating type of the isolates was either A1 or A2, and oogonia and oospores were measured as 28~31 ${\mu}{\textrm}{m}$ and 21~25 ${\mu}{\textrm}{m}$. PCR-RFLP analysis of rDNA of the five isolates resulted that restriction band patterns of the small subunit and ITS regions were identical to a perilla isolate of P. nicotianae, but distinct from P. cactorum and P. capsici. Cross inoculation tests showed that the five isolates had pathogenicity to lily, christmas cactus, anthurium, baby's breath and carnation with different degrees. However, each isolate showed stronger pathogenicity to its corresponding original host than others. Among five lily cultivars Georgia and Quririna were more susceptible than Napoli and others. This is first report of Phytophthora root and stem rot of lily, Christmas cactus, anthurium, baby's breath and monochoria in Korea.

• 한국자원식물학회지
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• 제12권2호
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• pp.139-144
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• 1999

광조사 방향 및 광도에 따른 몇 가지 배양식물체의 생장반응을 조사하였다. 광조사 방향은 배양실내 조명장치를 상방향 또는 측방에 설치하여 조사하였다. 딸기 배양식물체의 초장과 근장은 광조사 방향에 관계없이 차이가 없었으나, 엽면적, 생체중 및 건물중은 광도가 높을 수록 증가하였다. 감자 절간 배양에서도 광도가 높은 측방광 조사구에서 초장이나 근장은 감소하였으나, 줄기 및 뿌리는 굵기가 굵고 생체중과 건물중 그리고 엽면적도 증가하였다. 백합인편배양 역시 측방광 고광도 조사구에서 자구 형성이 많았고 생체중과 건물중 모두 증가하였다. 따라서 관행 하방광 조사보다 배양용기의 측면에서 광을 조사하여 광도를 높이고, 배양용기를 3단으로 적재할 수 있는 측방광 조사방법이 배양식물체의 생장을 촉진시켜 양질 배양묘 생산효율을 증대시켰다.

• 한국환경보건학회지
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• 제31권4호
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• pp.287-293
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• 2005

Pollen has been used for Prevention or treatment of certain diseases such as diabetes, arthritis, or cancer in traditional medicine. In addition, pollen is under investigation as a host cell for a gene expression. This study was undertaken to evaluate the immunologic safety of pollen intake. BALB/c mice were administered with 500, 50,5, or 0.5 mg/kg bw of lily pollen for five times a week for four weeks through gastric intubation. Comparing the control mice administered with distilled water, no significant changes were observed in body weight gain, weight of liver, spleen, lung, and his-topathological findings of liver and kidney of the mice groups administered with the pollen. Plasma level of IgG1, IgG2a, and IgE was not different among the groups. When splenic B lymphocytes were stimulated in vitro with lipopolysaccharides for 7 days, level of IgGl and IgGwa produced in the culture supernatants was not significantly different among the groups. Furthermore, no significant alteration was observed in IL-4 and $IFN{\gamma}$ producing ability with splenic T lymphocytes stimulated in vitro with phytohemagglutinins for 48 hours between the pollen-administered and the control mice. Overall, this study suggests that the lily pollen intake is Inducing no significant modulation of humoral and cell-mediated immunity in mice.

• 한국식품저장유통학회지
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• 제24권3호
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• pp.381-386
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• 2017

Ergocalciferol은 vitamin D의 활성을 가지는 것으로 알려져 있다. 본 연구에서는 팽이(Flammulina velutipes), 느타리 (Pleurotus ostreatus), 애느타리(Pleurotus ostreatus), 새송이(Pleurotus eryngii), 양송이(Agaricus bisporus), 표고(Lentinula edodes), 목이(Auricularia auricula-judae) 등 7종의 버섯을 대상으로 자외선 조사에 따른 ergocalciferol(EC, 비타민 $D_2$) 함량의 증진 효과를 조사하였다. 자외선을 조사하지 않은 버섯의 ergocalciferol 함량은 극히 낮았으나 자외선 조사에 의해 ergocalciferol 함량이 크게 증가하였으며, 자외선 A, B, C 중 특히 자외선 B가 EC 함량 증진에 효과적이었다. 버섯에서 ergocalciferol의 생성은 자외선에 직접 노출되는 부위 즉 깊이 1 mm 이내의 버섯 표면에서만 일어나는 것으로 조사되었다. 따라서 세절하지 않은 생 버섯에 자외선을 조사할 경우 팽이 버섯과 같이 체적에 비해 표면적인 넓은 종류의 버섯에서 ergocalciferol 생성이 많았고, 건조 및 세절할 경우에는 EC 생성의 기질인 ergosterol의 함량이 높은 버섯에서 ergocalciferol 생성이 많았는데, 새송이, 표고, 양송이 등이 이에 속하였다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.43-43
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• 2020

Plants regenerated from in vitro cultures are associated with chromosomal variations, which have been generally found in long-term culture. Reducing plant culture age is one of the ways to reduce genetic and epigenetic changes. The present study focused on the efficient in vitro propagation of lily cultivars and has intensified to speed up bulb propagation for cryopreservation. The multiplication process applied in this experiment uses starting material, which the newly small bulb formed from bulb-scales in two lily cultivars. The adventitious bulb from bulb-scale tissue cultured on three different media following Murashige and Skoog (MS) basal medium supplemented with 1 g/L Charcoal, MS medium containing 0.3 mg/L IAA and 0.4 mg/L BA hormone with or without Charcoal, respectively. After about seven weeks, there is little change in the number of newly propagated bulbs in small bulbs of the two media. Compared to the both mediums, the number of the propagated bulbs is increased 5 times in MS medium containing 0.3 mg/L IAA and 0.4 mg/L BA hormone without Charcoal. After about seven weeks, the results of the propagation showed that the number of the propagated bulbs is increased 5 times in MS medium containing 0.3 mg/L IAA and 0.4 mg/L BA hormone without Charcoal compared to the both mediums. The number of propagated bulbs ranged from 5 to 6 and 4 to 6 with an average of 5 in Tropicalpink and Greenstar cultivars, respectively. There is little change in the number of newly propagated bulbs in small bulbs of other media. The multiplication process applied in this study may save in vitro culture period and effort.

• 한국환경농학회지
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• 제42권4호
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• pp.396-407
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• 2023

The golden apple snail (Pomacea canaliculata) has been utilized as a natural and eco-friendly control of weeds in rice paddy fields. However, P. canaliculata can damage other crops. In this study, the effectiveness of plant extracts from various natural sources that are reportedly effective against pests in the control of P. canaliculata was investigated. The four plant extracts were effective against P. canaliculata and ranked in descending order as green tea seed (Camellia sinensis) > root of red spider lily (Lycoris radiata) > leaves of tobacco (Nicotiana tabacum) > root of sophora (Sophora flavescens). The mortality rate of P. canaliculata was increased using 200 to 2000 mg/kg of green tea seed powder. However, shrubby sophora root extract did not significantly increase the mortality rate. The LC₅₀ and LC₉₀ of green tea seed, tobacco leaves, shrubby sophora root, and red spider lily root were 900 and 2800 mg/L, 956 and 2320 mg/L, 2162 and 5325 mg/L, and 512 and 1054 mg/kg, respectively. The LC₅₀ and LC₉₀ of ground powder of C. sinensis, N. tabacum, S. flavescens and L. radiata were 248 and 646 mg/L, 403 and 733 mg/L, 409 and 905 mg/L, and 493 and 1141 mg/L, respectively. The findings indicate the remarkable control potency of green tea seeds against the golden apple snail. An organic material incorporating the four plant powders may help control green apple snail in an ecosystem-friendly manner.

• Current Research on Agriculture and Life Sciences
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• 제11권
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• pp.61-70
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• 1993

나리속(屬) 식물(植物)에 있어서 차광정도(遮光程度)에 따른 종(種) 혹은 품종간(品種間)의 광반응(光反應)의 차이(差異)를 구명(究明)하여 약광(弱光)에 둔감(鈍感)한 식물(植物)의 선발(選拔)과 차광(遮光)이 주아형성(珠芽形成)에 미치는 영향(影響)을 알아보고자 자연광처리(自然光處理)(대조품(對照品)) 및 약 30%, 50%, 75%, 90%의 차광(遮光) 조건하에서 실험하여 얻은 결과(結果)는 다음과 같다. 개화율(開花率)에 미치는 차광(遮光)처리의 효과는 'Roma'를 제외한 공시품종 모두 75% 차광(遮光)처리까지는 차광(遮光)정도가 심할수록 증가하는 경향(傾向)이었으나 95% 정도의 극심한 차광(遮光)처리구에서는 공시 품종 모두 매우 낮은 개화율을 보였다. 화수(花數)에 미치는 차광(遮光)처리의 효과도 이와 동일한 경향(傾向)이었다. 개화소요일수(開花所要日數)에 미치는 차광(遮光)처리의 영향을 보면 공시품종 모두 차광(遮光)이 심할수록 개화소요일수가 길어지는 경향(傾向)이었으나 'Roma'와 Tiger lily가 개체로 둔감(鈍感)하여 대조구와 7일의 차이를 보인 반면, 다른 품종은 12-13일 정도의 차이를 보였다. 공시품종 모두 본엽수(本葉數)에는 차광(遮光)이 그다지 큰 영향을 미치지 않았으나 Tiger lily와 'Connecticut king'에서는 차광율(遮光率)이 높아질수록 본엽수가 감소(減少)되었다. 포엽수(苞葉數)에 있어서는 공시 품종 모두 차광율(遮光率)이 높아질수록 감소(減少)되는 경향(京鄕)이었다. 초장(草長)과 절간(節間)은 차광율(遮光率)이 높아질수록 길어지는 경향(傾向)이었는데 'Georgia'가 가장 높은 신장율(伸長率)을 나타냈다. 주아(珠芽)형성과 생육 정도에 미치는 차광(遮光)의 영향을 보면 'Enchantment'와 Tiger lily는 차광율(遮光率)이 높아질수록 전체(全體) 주아수(珠芽數)와 주아중(珠芽重)이 감소(減少)되었다. 그러나 'Enchantment'의 경우 포엽부위(苞葉部位)에 발생된 주아(珠芽)의 중량(重量)이 타 부위에서 형성된 주아(珠芽)보다 약간 무거운 경향(傾向)을 보였다. 'Connecticut king'과 'Roma'의 주아형성(珠芽形成)은 차광(遮光) 75%와 90%처리구에서 재배한 식물의 줄기 상부(上部)에서만 관찰(觀察)되었다. 주아(珠芽)의 인편엽(隣片葉) 출엽율(出葉率)에 있어서는 'Enchantment'와 'Connecticut king'에서만 관찰(觀察)되었고, 특히 포엽(苞葉)부위에서 형성된 주아(珠芽)에서만 관찰되었는데 차광(遮光)정도가 강(强)할수록 출엽율(出葉率)이 양호(良好)하였다.