• 제목/요약/키워드: lignin determination

검색결과 21건 처리시간 0.032초

과일.채소중 식이섬유의 분석법 검토 및 함량 분석 (Determination of Dietary Fiber Content in Some Fruits and Vegetables)

  • 이경숙;이서래
    • 한국식품과학회지
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    • 제19권4호
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    • pp.317-323
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    • 1987
  • 국내의 공업적 등급의 시판용 효소제를 사용하며 AOAC에서 공인된 enzymatic-gravimetric 방법에 따라 과일 4종류(사과, 배, 복숭아, 감)와 채소 4종류(무우, 상추, 배추, 배추김치)에 대한 식이심유의 함량을 측정하였다. 국내의 시판용 효소제를 사용하였을 때 AOAC가 추천하는 정제된 효소제에 대하여 효소역가 1/10, 반응시간 2배의 조건을 채택하여 과일 채소 중 식이섬유의 함량을 측정할 수 있었다. 과일, 채소의 건조물중 식이섬유의 함량은 과일의 경우 총함량은 $9.4{\sim}28.8%$, non-cellulosic polysac-charide 함량은 $1.8{\sim}7.8%$, cellulose 함량은 $3.7{\sim}5.8%$, lignin 함량은 $1.3{\sim}11.3%$의 범위이었다. 채소의 경우 총함량은 $26.0{\sim}35.7%$, non-cellulosic polysac-charide 함량은 $12.3{\sim}14.4%$, celluose 함량은 $12.3{\sim}19.7%$, lignin 함량은 $1.4{\sim}7.4%$의 범위이었다. 과일, 채소의 건조물중 조섬유함량은 과일의 경우 $3.5{\sim}6.7%$, 채소의 경우 $9.1{\sim}13.8%$의 범위이었다. 따라서 과일, 채소중 조섬유함량은 식이섬유의 $12{\sim}50%$만이 정량되는 것으로 나타났다.

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Nuritive Evalution of Forage Plants Grown in South Sulawesi, Indonesia

  • Nasrullah, Nasrullah;Niimi, M.;Akashi, R.;Kawamura, O.
    • Asian-Australasian Journal of Animal Sciences
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    • 제16권5호
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    • pp.693-701
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    • 2003
  • In order to evaluate the nutritive value of the forage plants in South Sulawesi, Indonesia, 266 samples (61 grasses and 65 legumes grown in the dry season, 60 grasses and 80 legumes grown in the rainy season) were collected from the highland and lowland in 1998 to 2000, and were subjected to the determination of chemical composition and digestibility. The least-squares analysis of variance demonstrated that the in vitro dry matter digestibility (IVDMD) of grasses was not significantly affected by season or altitude. On the other hand, the some proximate components and cell wall components were significantly affected by season and altitude including the season${\times}$altitude interaction. For the legumes, the in vitro neutral detergent fiber digestibility (IVNDFD) and cellulose content were significantly affected by season. On the other hand, the ether extract (EE) content was significantly affected by season and altitude. The interaction of the season${\times}$altitude for IVDMD, of the year${\times}$season for some proximate components and of the year${\times}$season and the season${\times}$altitude for some cell wall components were significant. These results indicate that the forages grown at highland in dry season have a relatively high quality. The means of the total digestible nutrient (TDN) content estimated from IVDMD in grasses and in legumes were 50.3% and 57.4%, respectively, and the crude protein contents were 7.7% and 17.6%, respectively. The correlation coefficients between IVDMD and the contents of crude fiber, neutral detergent fiber and acid detergent fiber were relatively high in all of forage plants, suggesting that these components would provide an accurate prediction of digestibility or TDN content. A close relationship between IVNDFD and lignin content indicates that the lignin would be the most accurate predictor of cell wall digestibility.

골목성분(?木成分)의 분해(分解) (Degradation of Quercus serrata by Lentinus edodes (Berk) Singer)

  • 윤병호
    • Journal of the Korean Wood Science and Technology
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    • 제6권1호
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    • pp.3-7
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    • 1978
  • 중간부후균(中間腐朽菌)인 Lentinus edodes (Berk) Singer에 의(依)한 졸참나무의 성분(成分) 분해(分解)에 미치는 영향(影響)을 조사(調査)하기 위(爲)해 건전재(健全材) 종균(種菌)을 접종(接種)한 후 2년(年)및 5년(年)을 경과(經過)한 3종류(種類)의 골목을 시료(試料)로 사용(使用)한 결과(結果)는 다음과 같다. 1) 탄수화물(炭水化物) 부분(部分)에 주(主)로 분해(分解)를 일으키며, 알카리 추출물(抽出物)의 증대(增大) 및 결정화도(結晶化度)의 감소(減少)는 목재(木材)의 변질분해(變質分解) 사실(事實)을 더욱 확실(確實)히 한다. 2) 크라손리그닌의 정량(定量), 켈 여과(濾過), 니크로벤젠 산화등(酸化等)의 결과(結果)에 의(依)하면 리그닌도 대단(大端)히 변질(變質) 분해(分解)받고 있다고 생각할 수 있다. 한편MWL의 메톡실기(基)의 감소(減少), 카르복실기의 증가(增加)는 탈(脫)메칠화(化)와 산화(酸化)가 평행(平行)한다고 설명할 수 있다. 3) 니트로벤젠산화(酸化)의 결과(結果) S/V 치(値)가 적은 것으로 보아 시링길핵(核) 단위(單位)가 바닐린 보다 비교적(比較的) 분해(分解)하기 쉽다고 생각된다. 또한 알데이하이드기(基)에 비(比)해 카르복실(酸)(시링길산(酸))의 증가(增加)는 생분해(生分解)에 의(依)해 산화(酸化)가 진행(進行)하고 있다는 것을 뜻한다.

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더덕과 인삼의 식이섬유소 함량의 측정 (Determination of Dietary Fiber Contents in Dodok ( Codonopsis lanceolata traut (Beneth et Gook)) and Ginseng (Panax ginseng C. A. Meyer))

  • 김은희;김지영;박찬경;맹영선
    • 한국식품조리과학회지
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    • 제8권3호
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    • pp.247-253
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    • 1992
  • The total dietary fiber (TDF) contents in cultivated dodok, wild dodok and ginseng were determined by AOAC method and Mongeau et al's method. Also, the nonstarch polysaccharide (NSP) contents of them were analyzed by Englyst et al's gas chromatographic (GC) method. The TDF values by AOAC method and Mongeau et al's method were 34.50% and 35.92% for wild dodok, 46.40% and 47.55% for cultivated dodok, and 14.93% and 14.03% for ginseng, respectively. The ratios of soluble dietary fiber (SDF) contents to TDF contents by Mongesu et al's method were 64.17% for wild dodok, 75.77% for cultivated dodok, and 53.74% for ginseng, respectively. The major sugar constituents of NSF in wild dodok, cultivated dodok and ginseng by GC were glucose, galactose and uronic acid. The Enalyst's TDF contents, i.e., NSP plus lignin contents in wild dodok, cultivated dodok, and ginseng were 20.65%, 20.03% , and 9.72%, respectively.

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Biogas Production from Vietnamese Animal Manure, Plant Residues and Organic Waste: Influence of Biomass Composition on Methane Yield

  • Cu, T.T.T.;Nguyen, T.X.;Triolo, J.M.;Pedersen, L.;Le, V.D.;Le, P.D.;Sommer, S.G.
    • Asian-Australasian Journal of Animal Sciences
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    • 제28권2호
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    • pp.280-289
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    • 2015
  • Anaerobic digestion is an efficient and renewable energy technology that can produce biogas from a variety of biomasses such as animal manure, food waste and plant residues. In developing countries this technology is widely used for the production of biogas using local biomasses, but there is little information about the value of these biomasses for energy production. This study was therefore carried out with the objective of estimating the biogas production potential of typical Vietnamese biomasses such as animal manure, slaughterhouse waste and plant residues, and developing a model that relates methane ($CH_4$) production to the chemical characteristics of the biomass. The biochemical methane potential (BMP) and biomass characteristics were measured. Results showed that piglet manure produced the highest $CH_4$ yield of 443 normal litter (NL) $CH_4kg^{-1}$ volatile solids (VS) compared to 222 from cows, 177 from sows, 172 from rabbits, 169 from goats and 153 from buffaloes. Methane production from duckweed (Spirodela polyrrhiza) was higher than from lawn grass and water spinach at 340, 220, and 110.6 NL $CH_4kg^{-1}$ VS, respectively. The BMP experiment also demonstrated that the $CH_4$ production was inhibited with chicken manure, slaughterhouse waste, cassava residue and shoe-making waste. Statistical analysis showed that lipid and lignin are the most significant predictors of BMP. The model was developed from knowledge that the BMP was related to biomass content of lipid, lignin and protein from manure and plant residues as a percentage of VS with coefficient of determination (R-square) at 0.95.This model was applied to calculate the $CH_4$ yield for a household with 17 fattening pigs in the highlands and lowlands of northern Vietnam.

리그노셀룰로오스 생물학적 분해를 이용한 간단한 양송이 육종효율 우수 균주 선발 (A simple screening method using lignoceullulose biodegradation for selecting effective breeding strains in Agaricus bisporus)

  • 오연이;남윤걸;장갑열;공원식;오민지;임지훈
    • 한국버섯학회지
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    • 제15권3호
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    • pp.134-138
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    • 2017
  • 양송이의 국내 생산량은 9,732톤으로 5번째로 가장 많이 생산되는 버섯이다. 양송이가 속한 주름버섯속은 리그노셀룰로오스 분해력을 가진 버섯으로 알려져 있다. 양송이의 영양생장과 생식생장동안 기본적으로 셀룰로오스, 해미셀룰로오스, 리그닌 성분의 분해는 변화한다. 우리는 양송이에서 celluase, xylanase, ligninolytic enzyme의 세포외효소 활성도로 효과적인 생물학적 분해력을 가진 균주를 선발하였다. 각 효소의 생물학적 분해는 0.5% CMC-MMP ((malt-mops-peptone), 0.5% Xylan-MMP, 0.5% ligin-MMP 배지에서 14일 동안 균주들을 배양하여 측정하였다. 그리고 배양된 균사체는 0.2% trypan blue에 염색되었다. MMP배지내 각 효소의 생물학적 분해의 효율성에 따라 선발된 균주 18점은 6개로 그룹화되었다. 또한 이 균주들은 볏짚발효퇴비배지에서의 균사생장과 세포외효소 활성도의 연관성을 알아보기 위해 볏짚발효퇴비배지에서 10일, 20일 각각 배양되었다. 본 연구로 ligninolytic enzyme 활성도가 볏짚발효퇴비배지에서의 균사배양과 상관도가 가장 높아 균주내 ligninolytic enzyme의 활성도 비교로 볏짚발효퇴비배지에서 균사배양이 우수한 균주를 간단하게 선발 할 수 있다고 사료된다.

해조가공품의 Dietary Fiber 함량과 물리적 특성 (Determination and Physical Properties of Dietary Fiber in Seaweed Products)

  • 김선희;박희연;박원기
    • 한국식품영양과학회지
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    • 제17권4호
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    • pp.320-325
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    • 1988
  • 전남 서남해안에서 생산되는 수출용 해조가공품인 마른미역, 마른다시마, 찐톳, 마른김, 마른파래, 한천을 시료로 하여 Dietary Fiber의 함량, 보수성 및 밀도를 측정한 결과는 다음과 같다. 1. Dietary Fiber 중 Neutral Detergent Fiber는 찐톳이 41.52%로 가장 많고, 한천이 0.05%로 가장 적었으며, Acid Detergent Fiber도 찐톳이 26.20%로 가장 많고, 한천이 0.01%로 가장 적게 나타났다. Lignin은 찐톳이 6.94%로 가장 많고, 마른김과 한천은 거의 존재하지 않았으며, Hemicellulose는 마른김이 25.79%로 가장 많고, 한천이 0.04%로 가장 적게 나타났다. 또한 Cellulose는 찐톳이 19.26%로 가장 많고 한천이 0.01%로 가장 적게 나타났다. 보수성은 마른미역이 $16.6g{\cdot}waer\;/\;g{\cdot}A.D.P$로 가장 큰 값을 보였고, 기타 시료는 $6.1{\sim}8.5g{\cdot}water\;/\;g{\cdot}A.D.P)$로 비슷하였으며, 침지시간 및 입자크기별 보수성의 변화는 크게 나타나지 않았다. 밀도는 Direct density가 $500{\sim}806mg/ml$Bulk density가 $0.562{\sim}0.833g\;/\;cc$Hydrated density가${1.4{\sim}2.01g\;/\;ml$Hydrated volume expansion이$102{\sim}160%$였으며, Direct density와 Bulk density의 값은 대체로 상응하였다.

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Phenolic Glycosides Isolated from Safflower (Carthamus tinctorius L.) Seeds Increase the Alkaline Phosphatase (ALP) Activity of Human Osteoblast-like Cells

  • Kim, Dong-Hyun;Lee, Jin-Hee;Ahn, Eun-Mi;Lee, Youn-Hyung;Baek, Nam-In;Kim, In-Ho
    • Food Science and Biotechnology
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    • 제15권5호
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    • pp.781-785
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    • 2006
  • The chemical compositions of the seeds of the safflower (Carthamus tinctorius L.) plant were evaluated to determine possible compound having proliferative effects on human osteoblast cells. Three-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) test and alkaline phosphatase (ALP) activity were used to assess the effects of the isolates on the human osteoblast-like line (Saos-2). Activity guided fractionation led to the isolation of ALP activating lignin and alkaloid glycosides through the extraction of the seeds, solvent partitioning and repeated silica gel and octadecyl silica (ODS) column chromatographic separations. The data from Nuclear Magnetic Resonance (NMR), Mass (MS), and Infrared (IR) analyses enabled the determination of the chemical structure and characterization of two compounds as a tracheloside and an N-(p-coumaroyl)-serotonin mono-${\beta}$-D-glucopyranoside. These two compounds showed respectively $149.2{\pm}4.2$ and $138.9{\pm}3.5%$ ALP activity compared to the control when evaluated at a concentration of $100\;{\mu}g/mL$.

Impact of Seepage from Land Treatment of Pulp and Paper Effluent on Water Quality and Aquaculture

  • Wirojanagud, W.;Tantemsapaya, N.;Chalokpanrat, P.;Suwannakom, S.
    • Environmental Engineering Research
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    • 제15권3호
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    • pp.163-166
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    • 2010
  • Pulp and paper mill wastewater has been treated by biological treatment, but the secondary effluent still contains high lignin, chemical oxygen demand, color and total dissolved solids. Tertiary treatment by land application, referred to as 'Project Green,' has been implemented to treat such high quantities of undesirable matters. The impacts of seepage from Project Green diffusing into receiving streams on the water quality and fish pen aquaculture were studied via the integration of technical and social approaches. The determination of the water quality was performed for 13 sampling stations along the receiving stream, including the Chot stream, Chot lagoon and the Pong River. The water quality was generally at normal levels, with the exception of total dissolved solids. The levels of matter were higher at the Chot stream, but became more diluted at the Chot lagoon and the Pong River, respectively. The social approach was conducted through the voluntary participation of the villagers as research assistants for the fish aquaculture study. Fish could grow at three fish pens within the study sites at the location of Project Green, the Chot lagoon and the Pong River. Fish growth at the Chot lagoon was better at the site of Project Green and the Pong River. The integration of technical and social approaches was a meaningful tool not only for the technical feasibility but in helping to solve the conflict between the community and industry.

Effect of Selective Consumption on Intake, Diet Selectivity and Passage Kinetics of Wheat Straw by Barbari Goats

  • Biswal, B.;Hasan, Q.Z.;Sharma, K.;Dutta, N.
    • Asian-Australasian Journal of Animal Sciences
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    • 제13권7호
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    • pp.913-917
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    • 2000
  • Sixteen adult Barbari bucks were divided into 4 homogeneous groups, each group was offered wheat straw (WS) at levels 75, 100, 125 and 150 per cent of ad libitum intake in a factorial randomized complete block design. All goats were uniformly fed a supplement ($13.5g\;DM/kg\;W^{0.75}$) to fulfill their requirement for protein at maintenance level. The experimental feeding of goats at different levels for 21 days was followed by a digestion trial of 6 days and determination of rate of passage of digesta for 144 h duration, respectively. Intake ($g/kg\;W^{0.75}$) of dry matter (DM), organic matter (OM), digestible DM and digestible OM increased with increase in amount of WS offered. The levels of WS refusals for obtaining upper limit of intake was found to be above 30% of DMO. Concentration of ADF and lignin was significantly lower and CP was higher (p>0.05) in food ingested relative to food offered or refused in response to increasing allowance of WS. Dietary means of particulate rate of passage from rumen, transit time and total mean retention time ranged from 0.032 to 0.036/h, 19.10 to 21.72/h and 58.61 to 61.53/h respectively and did not show significant differences (p>0.05) irrespective of dietary level of WS. The results suggest that DMI and quality of ingested WS would improve with higher rates of offer and refusals (${\geq}30%$ of DMO) without any pronounced effect on passage kinetics of digesta, because of the greater opportunity afforded to goats for selective feeding.