• 대한임상검사과학회지
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• 제47권4호
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• pp.273-278
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• 2015

이 연구의 목적은 임상 Candida albicans의 바이오필름억제을 위한 Rhadachlorin과 적색발광다이오드를 이용한 광역학치료의 체외적 효과를 평가하고자 하였다. Candida albicans 부유액을 $9{\times}10^8CFU/mL$을 5% 당이 함유된 YNB배지에 준비하였다. Candida albicans의 바이오필름은 96웰 플레이트에 100 uL씩 분주후 3시간 배양하고 상층액을 제거하였다. 각 웰에 $50{\mu}g/mL$ 부터 $0.39{\mu}g/mL$ 농도로 Rhadachlorin을 부착된 세포위에 처리하였다. 30분간 배양 후 빛을 30, 60, 90분을 630 nm 광원의 LED를 이용하여 조사하였고, 이때 각각의 에너지밀도는 14, 29, $43J/cm^2$이였다. 모든 상층액을 제거후에 건조시켰다. 부착된 세포를 safranin O로 염색하였다. 490 nm 파장으로 마이크로플레이트 리더로 흡광도를 측정하였다. 또한, 광감작제포화에 의해 Candida albicans의 형광시그널을 관찰하였다. 결론적으로 확연한 억제는 Rhadachlorin의 농도 $50{\mu}g/mL$과 630 nm LED치료에서 Candida albicans의 바이오필름의 72.5%가 감소되었다. 또한 광감작제 Rhadachlorin은 Candida albicans의 30분에서 충분한 축적을 보였다. 전체적으로 이 결과들은 바이오필름형성의 억제는 Rhadachlorin의 농도에 의존되었다. 이 결과들은 Rhadachlorin과 630 nm LED의 광역학치료는 Candida albicans의 바이오필름 억제가 가능한 것으로 제의된다.

• 한방재활의학과학회지
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• 제20권4호
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• pp.73-81
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• 2010

Objectives : The purpose of this study is to establish literatural evidence about thermotherapy and cryotherapy for Korean medicine through literatural review. Methods : Applicable paragraphs which were related to the thermotherapy and cryotherapy of cutaneous and muscle meridian were phrased from in "Yibujicheng(醫部集成) and "Dongyibaojian(東醫寶鑑)" where were archiving of Oriental or Korean medicine literatures. Searched paragraphs were analysed for establishing historical and theoretical bases of thermotherapy and cryotherapy in Korean medicine. Results : Thermotherapy of cutaneous and muscle meridian(經皮經筋溫熱療法) such as hot pack, warm water therapy, paraffin bath, ultrasound is originated from yu(慰) warm water(溫水) hot water(熱水). Matching indications are various pain conditions(caused by coldness(寒), hard-work(僗若), extravasated blood(瘀血), inflammatory skin disease, frostbite and several internal diseases. It also treats gynecological diseases and facial palsy. Diathermic therapy on acupuncture points(穴位照射溫熱療法) such as infra-red, microwave, shortwave is originated from huolu(火爐), wenlu(溫爐), xianglu(香爐), lamp light(燈火). Its objective is to improve the effects of herb medicine by aiding sweating or to treat the residual symptoms of fever disease or to care skin disease and pain from bone fracture, contusion. Cryotherapy of cutaneous and muscle meridian(經皮經筋溫寒冷療法) such as ice pack, ice spray, iced whirpool, cool water bath is originated from lengfu(冷敷), lengtie(冷貼), lengshiyu(冷石熨). Matching indications are contusions, animal bite injury, corn(肉刺) and (淋病), eye disease, nasal bleeding, hemorrhoid, inflammatory skin disease and chicken pox. Conclusions : Thermotherapy and cryotherapy of cutaneous and muscle meridian(經皮經筋溫冷療法) are the treatments which were used in Korean medicine from the ancient Korean medicine. As scientific equipments were originated from yu(慰), huolu(火爐), wenlu(溫爐), xianglu(香爐), lamp light(燈火). lengfu(冷敷), lengtie(冷貼), lengshiyu(冷石熨). It can be said that these are elements of Korean medicine. More rigorous studies are needed to establish clinical evidence about not only thermotherapy and cryotherapy but also the other physiotherapy of Korean medicine.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2010년도 춘계학술대회
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• pp.581-582
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• 2010

광 의료 분야 중 저출력레이저 치료에 사용되는 레이저와 같은 특정 파장을 발생하고 레이저보다 안정적으로 사용이 가능한 고휘도 LED를 조사하여 광 의료 분야에 적용할 수 있는 시스템을 개발하였다. 광조사 시스템은 마이크로 컨트롤러, FPGA, TLC5941 및 고휘도 LED를 이용하여 설계 및 제작하였으며 광조사 시간, 강도, 주기 등 세포 증식에 영향을 칠 수 있는 다양한 변수들의 제어가 가능하도록 하였다. 또한 세포 배양에 사용되는 인큐베이터 내부의 조사가 용이하도록 탈부착이 가능한 모듈화 된 LED를 설계 제작하여 세포 증식에서 발생 가능한 생물학적 변수를 최소화하였다.

• 생체재료학회지
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• 제22권4호
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• pp.303-310
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• 2018

Background: Photodynamic therapy (PDT) is photo-treatment of malignant or benign diseases using photosensitizing agents, light, and oxygen which generates cytotoxic reactive oxygens and induces tumour regressions. Several photodynamic treatments have been extensively studied and the photosensitizers (PS) are key to their biological efficacy, while laser and oxygen allow to appropriate and flexible delivery for treatment of diseases. Introduction: In presence of oxygen and the specific light triggering, PS is activated from its ground state into an excited singlet state, generates reactive oxygen species (ROS) and induces apoptosis of cancer tissues. Those PS can be divided by its specific efficiency of ROS generation, absorption wavelength and chemical structure. Main body: Up to dates, several PS were approved for clinical applications or under clinical trials. $Photofrin^{(R)}$ is the first clinically approved photosensitizer for the treatment of cancer. The second generation of PS, Porfimer sodium ($Photofrin^{(R)}$), Temoporfin ($Foscan^{(R)}$), Motexafin lutetium, Palladium bacteriopheophorbide, $Purlytin^{(R)}$, Verteporfin ($Visudyne{(R)}$), Talaporfin ($Laserphyrin^{(R)}$) are clinically approved or under-clinical trials. Now, third generation of PS, which can dramatically improve cancer-targeting efficiency by chemical modification, nano-delivery system or antibody conjugation, are extensively studied for clinical development. Conclusion: Here, we discuss up-to-date information on FDA-approved photodynamic agents, the clinical benefits of these agents. However, PDT is still dearth for the treatment of diseases in specifically deep tissue cancer. Next generation PS will be addressed in the future for PDT. We also provide clinical unmet need for the design of new photosensitizers.

• 대한물리치료과학회지
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• 제6권3호
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• pp.63-82
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• 1999

Recent studies have revealed that Nitric oxide(NO) was one of the demonstration for the physiological regulator, endothelial derived relaxing factor(EDRF) and that NO was produced by ultraviolet irradiation in human. Thus, the present author have carried out a experimental study on the change of hematological, histological value of ultraviolet irradiation in sprague-dawley rats. The subjects were divided into four groups of ten rats each selected at random. There were 4 groups: 1. no irradiation control; 2. ultraviolet $75mJ/cm^2$ irradiation group; 3. ultraviolet $150mJ/cm^2$ irradiation group; 4. ultraviolet $225mJ/cm^2$ group. After a irradiation, hematological and histological tests were performed to observe erythrocyte, hemoglobin, hematocrit, MCH, MCHC, MCV, $O_2$ saturation, pH, $PO_2,\;PCO_2$ value and to observe histological changes. In hematological tests, erythrocyte, hemoglobin, hematocrit significantly increased in $75mJ/cm^2$ than control group and more $150mJ/cm^2$ ultraviolet irradiation group respectively. Also In blood gas tests, $PO_2$ significantly increased in $75mJ/cm^2$ and more $150mJ/cm^2$ group than control group. Whereas $PCO_2$ significantly decreased in $75mJ/cm^2$ and more $150mJ/cm^2$ group than control group (Duncan-Tukey test, P<0.05). In histological tests, control and $75mJ/cm^2$ group unchanged, but more $150mJ/cm^2$ group changed that it was cytolysis, cytotoxic effect, acanthosis, proliferation of keratinocyte, appearance of amorphous cell and pyknotic nucleus, production of sunburn cell. In conclusion, the present author results support the importance of the relation between NO effect and hematological, histological value by ultraviolet B irradiation.

• 대한소아치과학회지
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• 제47권2호
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• pp.120-127
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• 2020

이 연구의 목적은 치면착색제인 10 - 20 mM erythrosine을 광감각제로 사용한 광역동 치료의 항균 효과를 평가하는 것이다. Streptococcus mutans, Lactobacillus casei, Candida albicans를 포함하는 다종 우식원성 세균막을 hydroxyapatite disc에 형성하였다. 광감각제로 20 μM, 10 mM, 20 mM erythrosine을 3분간 적용 후 24초 동안 광조사를 시행하였다. 각 실험군의 Colony-forming unit (CFU)을 측정하고 세균막을 Confocal laser scanning microscopy (CLSM)을 이용하여 관찰하였다. 10 - 20 mM erythrosine을 광감각제로 사용한 광역동 치료 실험군에서 CFU의 현저한 감소가 관찰되었고, 그 결과는 CLSM 관찰에서도 확인되었다. 이번 연구를 통해 치과 임상에서 사용되는 치면착색제를 광감각제로 이용한 광역동 치료의 높은 항균효과를 확인할 수 있었다.

• 대한한방내과학회지
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• 제38권4호
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• pp.531-540
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• 2017

Objectives: This study discusses the effects of constitution therapy on adolescent ulcerative colitis. Methods: A 12-year-old male patient was treated with western medication for six months and herbal medicine for one year and six years; however, his abdominal pain, mucousy stool, bloody stool, and diarrhea persisted. He was diagnosed as having Taeumin according to Sasang constitution classification and treated with Sasang constitutional medicine (i.e., Yeuldahanso-tang and food restrictions based on constitutional medicine theory). Before the first treatment, his Pediatric Ulcerative Colitis Activity Index (PUCAI) score was 35, but this score decreased to 15 within six months and was maintained at 10 by 18 months of treatment. He took the herbal medication for 38 months, and his PUCAI score was 0 at the end of treatment. His liver functioned normally despite long-term drug use, and a follow-up colonoscopy showed no ulcerative colitis except melanosis, which was presumed to be caused by the herbal medicine. Ulcerative colitis in pediatric adolescents requires treatment with pharmacotherapy and dietary control based on constitutional medicine to maintain disease remission. Conclusions: Constitutional therapy is effective for treating adolescent ulcerative colitis. More clinical data are needed for patients with ulcerative colitis.

• The Journal of Korean Physical Therapy
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• 제23권6호
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• pp.77-84
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• 2011

Purpose: The objectives of this study were to determine the enhancing effect of iontophoresis method as it transdermally deliver methylene blue (MB) using visual examination, in terms of penetration depth and tissue distribution in the skin, and to determine the effect of application duration on the efficacy of iontophoresis. Methods: Twenty-four male Sprague-Dawley rats were randomly divided into 5-, 10-, 20-, and 40-minute groups. These rats were exposed to either topical or anodic iontophoresis of 1% MB using a direct current of $0.5mA/cm^2$ for 5, 10, 20, and 40 minutes. Using cryosections of rat tissues, the penetration depth of MB was measured using light microscopy. Results: Significant differences in the penetration depth (F=54.20, p<0.001) were detected among the four groups. Post hoc comparisons of the penetration depth of MB data pooled across groups showed no significant difference between all topical application groups and 5-minute iontophoresis group, but did reveal a significant difference in the penetration depth between all topical application groups and 5-minute iontophoresis group versus 10-minute group, between the 10-minute and 20-minute group, and between the 20-minute and 40-minute iontophoresis group (p<0.05). Conclusion: The results demonstrate that iontophoresis enhances transdermal delivery of MB across stratum corneum of skin barrier by visual examination. Furthermore, the penetration depth of iontophoretic transdermal delivery of MB was dependent on the application duration. The duration of iontophoresis is one of the important factor in the efficacy of iontophoresis application.

• 디지털융복합연구
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• 제15권9호
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• pp.435-441
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• 2017

본 연구는 미국에 거주하고 있는 한인 노인을 대상으로 현재 뉴욕 시에서 시행하고 있는 데이케어 프로그램(Adult Day Care Program)의 일환인 치료 레크리에이션의 신체적, 정신적 효과를 평가하고자 시행되었다. 연구 방법은 비동등성 대조군 사후 실험 연구로 2017년 2월부터 4월까지 진행되었다. 본 연구에 참여한 대상자는 실험군 35명, 대조군 35명이었으며 이 중 66명의 자료를 분석하였다. 본 연구에 참여한 대상자의 평균연령은 79.4세 이었으며 미국에 거주한 기간은 평균 24.6년 이었다. 치료 레크리에이션 프로그램은 데이케어센터에서 제공하는 프로그램으로서 본 연구결과, 데이케어센터 치료 레크리에이션에 참여한 노인의 일상생활 수행능력, 우울 및 외로움에 효과가 있는 것으로 나타났다. 따라서 향후 문화적 감수성을 고려한 노인 대상 다양한 치료 레크리에이션 프로그램이 개발, 운영되어져야 할 것이며 이에 대한 효과를 평가하기 위하여 근거기반 연구가 수행되어져야 할 것이다.

• 한국전문물리치료학회지
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• 제13권4호
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• pp.79-86
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• 2006

The purpose of the current experiment was to describe interlimb coordination when swing limb conditions are being manipulated by constraining step length or by adding a 5 or 10 pound weight to the swing limb distally. Subjects were asked to begin walking with the right limb to land on the primary target (normal step length) that is 10 cm in diameter. However, if, during movement, the light was illuminated, then the subject had to step on one of the secondary targets (long and short step length). These three step length conditions were repeated while wearing a 5 pound ankle weight and then when wearing a 10 pound ankle weight. Ground reaction force (GRF) data indicated that there were changes in the forces and slopes of the swing and stance Fx GRFs. Long stepping subjects had to increase the propulsive force required to increase step length. Consequently, swing and stance toe-off greatly increased in the long step length condition. Short step length subjects had to adequately adjust step length, which decreased the speed of gait initiation. Loading the swing limb decreased the force and slope of the swing limb. Swing and stance toe-off was longest for the long step length condition, but there was a small difference of temporal events between no weight and weight condition. It appears that subjects modulated GRFs and temporal events differently to achieve the peak acceleration force of the swing and stance limb in response to different tasks. The findings from the current study provide preliminary data, which can be used to further investigate how we modulate forces during voluntary movement from a quiet stance. This information may be important if we are to use this or a similar task to evaluate gait patterns of the elderly and patient populations.