• 제목/요약/키워드: light microscopy electron microscopy

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Light and Electron Microscopic Characterization of Husk from Korean Rice

  • Adya P. Singh;Park, Byung-Dae;Wi, Seung-Gon;Lee, Kwang-Ho;Yoon, Tae-Ho;Kim, Yoon-Soo
    • Plant Resources
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    • v.5 no.2
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    • pp.95-103
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    • 2002
  • Microscopic techniques were used to observe the microstructure of rice husk. Microscopic examination showed that two main components of husk, lemma and palea consisted of outer epidermis, layers of fibers, vascular bundles, parenchyma cells, and inner epidermis, in sequence from the outer to the inner surface. The outer epidermal walls were extremely thick, highly convoluted and lignified. The underlying fibers were also thick-walled and lignified. Parenchyma cells were thin-walled and unlignified. Inner epidermal cells were also unlignified. The outer surface of both lemma and palea were conspicuously ridged, but the lower surface had a flat appearance. As part of a detailed study to characterize rice husk using microscopic and micro-analytical techniques, distribution of silica was also examined, and is presented elsewhere. Rice husk can potentially be used as a raw material for making composite products and the observations presented here form valuable background information for our future work related to product development.

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Development of Hollow Fibers for the Controlled Release of Drugs

  • Feijen, J.;Eenink, M.J.D.;Olijslager, J.;Schakenraad, J.M.;Nieuwenhuis, P.;Molenaar, I.
    • Journal of Biomedical Engineering Research
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    • v.7 no.2
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    • pp.111-112
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    • 1986
  • Radiation-induced fibrosarcoma tumors were grown on the flanks of C3H mice. The mice were divided into two groups. One group was injected with Photofrin II, intravenously (2.5mg/kg body weight). The other group received no Photofrin II. Mice from both groups were irradialed for approximately 15 minutes at 100, 300, or 500 mW/cm2 with the argon (488nm/514.5 nm), dye(628nm) and gold vapor (pulsed 628 nm) laser light. A photosensitizer behaved as an added absorber. Under our experimental conditions, the presence of Photolfrin II increased surface temperature by at least 40% and the temperature rise due to 300 mW/cm2 irradiation exceeded values for hyperthermia. Light and temperature distributions with depth were estimated by a computer model. The model demonstrated the influence of wavelength on the thermal process and proved to be a valuable tool to investigate internal temperature rise.

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History of Microscope from the Magnifying Glass to the Field Emission Electron Microscope (돋보기에서 FE까지 현미경의 변천사 - 생명과학적 관점에서 -)

  • Park, Chang-Hyun;Yeom, Mi-Jung;Uhm, Chang-Sub
    • Applied Microscopy
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    • v.33 no.2
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    • pp.93-104
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    • 2003
  • Since the Ptolemaeos' discovery that glass has magnifying power, human desire to see the unseen with naked eyes has lead to the inventions of a series of microscopes. Since the Janssen's first compound microscope in 1595, through the Abbe's non-aberration microscopy, various microscopes using different principles are now being used in various biomedical researches. The discovery of electron by Thompson in 1897 has lead to the first invention of microscope using electron as an illumination source, the electron microscope, in 1931. Now we can see the objects as close as 0.05 nm using 1 MV FE-TEM constructed in 2000. In this review, the authors reviewed the predecessors efforts to develop better microscopes.

Synthesis of GaN nanowires using thermal chemical vapor deposition (열화학기상증착법을 이용한 GaN nanowire 합성)

  • 류승철;이태재;이철진
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2001.07a
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    • pp.931-934
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    • 2001
  • GaN nanowires has much interest as one-dimensional materials for blue light LED. GaN-based materials have been the subject of intensive research for blue light emission and high temperature/high power electronic devices. In this letter, the synthesis of GaN nanowires by the reaction of mixture of GaN nanowires by the reaction of mixture of Ga meta and GaN powder with NH$_3$ using thermal chemical vapor deposition is reported. X-ray diffraction, energy dispersive x-ray spectrometer, scanning electron microscopy, and transmission electron microscopy indicate that those GaN nanowires with hexagonal wurtzite structure were about 60nm in diameter and up to several hundreds of micrometers in length.

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Synthesis and Characterization of CdSe/graphene Nanocomposites and their Catalytic Reusability Studies under Visible Light Radiation

  • Ali, Asghar;Oh, Won-Chun
    • Journal of the Korean Ceramic Society
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    • v.52 no.6
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    • pp.502-507
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    • 2015
  • We examined the photo catalytic activity and catalytic recyclability of CdSe/graphene nanocomposites fabricated via modified hydrothermal technique. The prepared composites were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) with energy dispersive X-ray (EDX), transmission electron microscopy (TEM), Raman spectroscopic analysis, and X-ray photoelectron spectroscopy (XPS). The photocatalytic behavior was investigated through decomposition of RBB as a standard dye under visible light radiation. Our results indicate that there is significant potential for graphene based semiconductor hybrids materials to be used as photocatalysts under visible light irradiation for the degradation of organic dyes from industry effluents.

Quantitative Analysis of Pulp fiber Characteristics that Affect Paper Properties (II) (종이의 물성에 영향하는 섬유특성의 정량적 해석(II))

  • 이강진;박종문
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.32 no.2
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    • pp.35-39
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    • 2000
  • Refining is very important process of fibers treatment for proper paper properties. An extent of refining is usually measured by freeness, although freeness gives complicated meanings. One of a direct way of studying the refining effects on pulp fibers is making photomicrographs of beaten fibers. The conventional microscopy like light microscopy(LM) and scanning electron microscopy(SEM) require to preserve the wet structure of pulp fibers morphologically since most of papermaking process is carried out almost entirely in water. Recently developed microscopy, especially confocal laser scanning microscopy(CLSM), offers the possibility of examining fully hydrated pulp fibers. Cross-sectional images of wet pulp fibers are also generated using optical sectioning by CLSM and image analysis in order to verify and quantify the extent of fiber wall swelling indicating the internal fibrillation. At low beating load such as 2.5 kgf, in the same freeness, breaking length is higher than that of high beating load such as 5.6 kgf. fiber wall thickness at low beating load is greater than that at high beating load. This result is accounted for the fact that internal fibrillation in the low beating load was high.

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Ultrastructure of the tympanic mucosa in experimentally produced serous otitis media (고양이에서 유발된 장액성중이염시 중이점막의 미세구조에 관한 연구)

  • Park, Soon-Il;Yoon, Kang-Mook;Shim, Sang-Yul
    • Applied Microscopy
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    • v.12 no.1
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    • pp.1-10
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    • 1982
  • Serous otitis media is closely related with auditory tube function, but its etiology and pathogenesis are not clearly defined yet. So we tried to prove the theory of hydrops ex vacuo via the experimental study with cats by means of obstructing the pharyngeal orfice of the auditory tube and observe the serial changes in tympanic mucosa through light and electron microscopy. The results are as follows; 1. We confirmed the production of serous otitis media with auditory tube obstruction and have a new understanding of auditory tube function in middle ear aeration. 2. The effusion in serous otitis media was produced from the next day of experiment and increased till the fourteenth day, but decreased after the spontaneous perforation of ear drum. 3. Through the light microscopy, we observed the increasement of the sercretory cells including goblet cells, epithelial hyperplasia, capillary proliferation and invasion of inflammatory cells. 4. Through the elctron microscopy, we observed the protrusion of secretory cells, blobs in cilia, loss of cilia, increasement of vesicles, vacuoles and dense bodies in ciliated cells and Invasion of inflammatory cells. With above results, we concluded that aeration through auditory tube is the most important factor in serous otitis media and presumed the effusion was secreted by secretory cells.

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Ultrastructure of the Rust Fungus Puccinia miscanthi in the Teliospore Stage Interacting with the Biofuel Plant Miscanthus sinensis

  • Kim, Ki Woo
    • The Plant Pathology Journal
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    • v.31 no.3
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    • pp.299-304
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    • 2015
  • Interaction of the the rust fungus Puccinia miscanthi with the biofuel plant Miscanthus sinensis during the teliospore phase was investigated by light and electron microscopy. P. miscanthi telia were oval-shaped and present on both the adaxial and abaxial leaf surfaces. Teliospores were brown, one-septate (two-celled), and had pedicels attached to one end. Transmission electron microscopy revealed numerous electron-translucent lipid globules in the cytoplasm of teliospores. Extensive cell wall dissolution around hyphae was not observed in the host tissues beneath the telia. Hyphae were found between mesophyll cells in the leaf tissues as well as in host cells. Intracellular hyphae, possibly haustoria, possessed electron-dense fungal cell walls encased by an electron-transparent fibrillar extrahaustorial sheath that had an electron-dense extrahaustorial membrane. The infected host cells appeared to maintain their membrane-bound structures such as nuclei and chloroplasts. These results suggest that the rust fungus maintains its biotrophic phase with most mesophyll cells of M. sinensis. Such a nutritional mode would permit the rust fungus to obtain food reserves for transient growth in the course of host alteration.

Ultrastructural Study on the Oocyte Maturation of Swordtail (Xiphophorus hellerii) (송사리과 Swordtail (Xiphophorus hellerii)의 난모세포 성숙과정에 관한 미세구조 연구)

  • Hwang, Woo-Sub;Kim, Wan-Jong;Reu, Dong-Suck
    • Applied Microscopy
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    • v.28 no.3
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    • pp.263-271
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    • 1998
  • Oocyte maturation of the swordtail (Kiphophorus hellerii) was investigated by light and electron microscopy. In the ovary of the swordtail, various staged oocytes were observed, Mature oocytes were located in ovarian cortex, meanwhile immature ones were positioned in ovarian medulla. The oocyte was surrounded by several structures or cells such as chorion, follicle cells, follicular theaca and ovarian epithelium, respectively, from the inside toward outside. Growing and maturing oocytes healed numerous microvilli which interconnected the oocyte and the follicle cells to communicate each other. The mature oocyte had the electron dense chorion which appeared to be ultrastructure of two layers and contained pore canals. Oocyte maturation was characterized by not only the enlarged cell size and well differentiated cell organelles, brit also the increases of fat droplets, pinocytotic vesicles and yolk granules.

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Ultrastructure of Endocrine Cells in the Midgut Epithelium of the Japanese Cockroach, Periplaneta japonica (집바퀴의 중장 상피조직내에 있는 내분비세포의 미세구조)

  • Yu, Chai-Hyeock
    • Applied Microscopy
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    • v.26 no.4
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    • pp.421-430
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    • 1996
  • The endocrine cells in the midgut epithelium of the Japanese cockroach, Periplaneta japonica were observed by the light and electron microscopy. The midgut epithelium of the last instar larva and adult cockroach consisted of principal columnar cells, regenerative cells, and secretory granular cells. Midgut endocrine cells were positioned basally as a cone-shaped single cell in the epithelium or underneath the regenerative crypt cells. When midgut epithelium grows and the cell composing it transforms, between the endocrine cells and regenerative cells were made desmosome type junction and large vesicular shaped stretches of loose contact. The endocrine cells were characterized by a clear cytoplasm with abundant Golgi complex and numerous secretory granules. The secretory granules in the cell were spherical and electron dense with their diameter of $200{\sim}400nm$. The secretory granules have been observed as discharged by exocytosis on the basal and lateral side of the cell.

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