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Development of In Vitro Seedling Screening Method for Selection of Resistant Rice Against Bakanae Disease (벼 키다리병 저항성 검정을 위한 기내 유묘 검정법 개발)

  • Lee, Yong-Hwan;Lee, Myeong-Ji;Choi, Hyo-Won;Kim, Sung-Taek;Park, Jin-Woo;Myung, Inn-Shik;Park, Kyung-Seok;Lee, Se-Weon
    • Research in Plant Disease
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    • v.17 no.3
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    • pp.288-294
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    • 2011
  • Rice bakanae disease, caused by the fungus Fusarium fujikuroi, is one of the most important rice diseases and distributed widely in Asia. Resistance screening system in rice field had been established. However, the evaluation results of the system vary according to the environmental conditions when the test is conducted. To develop precise and rapid evaluation method of disease resistance of rice to bakanae disease, in vitro screening system was attempted in this study. The six cultivars namely, 'Nampyeongbyeo', 'Junambyeo', 'Chucheongbyeo', 'Samcheonbyeo', 'Odaebyeo' and 'Hwasinbyeo' were tested. They were planted onto MS agar medium (10 ml) in test tube ($450\;mm{\times}{\phi}30\;mm$) and incubated at $25^{\circ}C$ and $28^{\circ}C$ in growth chamber under 12 hr light condition. Symptoms of over growth appeared a few days after seeding and then seedling were withered 2-3 weeks after over growth. The disease symptoms such as leaf dryness on top of rice were appeared in the 'Nampyeongbyeo' from 28 days at the concentration of $10^5$ spores/ml culturing at $28^{\circ}C$ and then withered completely 35 days after seeding. Whereas the other varieties withered entirely 19-23 days after seeding. Using the in vitro seedling screening method, 72 rice varieties were investigated to select resistant cultivar. Finally, two resistant cultivars ('Nampyeongbyeo' and 'Inwolbyeo') and seven moderately resistant cultivars ('Hwadongbyeo', 'Seokjeongbyeo', 'Samgwangbyeo', 'Sampyeongbyeo', 'Nonghobyeo', 'Heukjinjubyeo' and, 'Joanbyeo') were selected. If in vitro seedling screening method was used for evaluation of bakanae disease resistance, it would be completed within 35 days after sowing of rice seed.

Factors Related with Job Satisfaction in Workers - Through the Application of NIOSH Job Stress Model - (직장인의 직무만족도 관련요인 분석 - NIOSH의 직무스트레스 모형을 적용하여 -)

  • Kim, Soon-Lae;Lee, Bok-Im;Lee, Jong-Eun;Rhee, Kyung-Yong;Jung, Hye-Sun
    • Research in Community and Public Health Nursing
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    • v.14 no.2
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    • pp.190-199
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    • 2003
  • This study was conducted to determine the factors affecting job satisfaction in workers by using the Job Stress Model proposed by the National Institute for Occupational Safety and Health (NIOSH). Data were collected from December 1 to December 30, 1999. The subjects were 2,133 workers employed at 155 work sites, who were examined using NIOSH Job Stress questionnaire translated by the Korea Occupational Safety ${\pounds}|$ Health Academy and Occupational Safety ${\pounds}|$ Health Research Institute. SAS/PC program was used for statistical analysis using descriptive analysis. Pearson's correlation coefficient, ANOVA, and Stepwise multiple regression analysis. The results of this study were as follows. 1. According to general characteristics of the subjects, job satisfaction was high in those with less number of children. 2. By work condition, job satisfaction was higher in those who were working in a permanent job position, were working with regular time basis than with shift basis, were working in regular shift hours than in changing shift hours, were working for a short period, and were working less hours and overtime works per week. 3. In terms of physical work environment, job satisfaction was significantly related to 10 physical environmental factors. In other words, job satisfaction was high in workers who were working in an environment with no noise, bright light, temperature adjusted to an appropriate level during summer and winter, humidity adjusted to an appropriate level. well ventilation, clean air, no exposure to hazardous substance during work hour, overall pleasant work environment and not crowded work space. 4. By work-related factors, job satisfaction was high in those with less ambiguity about future job and role, high job control/autonomy, and less workload. On the other hand, job satisfaction was low in those with little utilization of competencies, and much role conflict at work and workload. 5. As for the relationships between job satisfaction and the non-work related factors, job satisfaction was high in workers who were volunteering at different organizations or active in religious activities for 5-10 hours per week. 6. In the relationships between job satisfaction and buffering factors, significantly positive correlations were found between job satisfaction and factors such as support by direct superior, support by peers, and support by spouse, friend and family. 7. There were nine factors that affected job satisfaction in the workers: age, number of children, work hours per week, noise, temperature at the work site during summer, uncomfortable physical environment, role ambiguity, role conflict, ambiguity in job future, work load, no utilization of competencies and social support from direct supervisor. These nine factors accounted for 26% of the total variance in the multiple regression analysis. In conclusion. the following are proposed based on the results of this study. 1. The most important physical environmental factors affecting job satisfaction in workers were noise, role ambiguity, and work load, suggesting a need to develop strategies or programs to manage these factors at work sites. 2. A support system that could promote job satisfaction is needed by emphasizing the roles of occupational health nurses who may be stationed at work sites and manage the factors that could generate job stress. 3. Job satisfaction is one of the three acute responses to stress proposed in NIOSH job stress model (job satisfaction. physical discomfort and industrial accidents). Therefore, further studies need to be conducted on the other two issues.

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Investigation of O4 Air Mass Factor Sensitivity to Aerosol Peak Height Using UV-VIS Hyperspectral Synthetic Radiance in Various Measurement Conditions (UV-VIS 초분광 위성센서 모의복사휘도를 활용한 다양한 관측환경에서의 에어로솔 유효고도에 대한 O4 대기질량인자 민감도 조사)

  • Choi, Wonei;Lee, Hanlim;Choi, Chuluong;Lee, Yangwon;Noh, Youngmin
    • Korean Journal of Remote Sensing
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    • v.36 no.2_1
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    • pp.155-165
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    • 2020
  • In this present study, the sensitivity of O4 Air Mass Factor (AMF) to Aerosol Peak Height (APH) has been investigated using radiative transfer model according to various parameters(wavelength (340 nm and 477 nm), aerosol type (smoke, dust, sulfate), aerosol optical depth (AOD), surface reflectance, solar zenith angle, and viewing zenith angle). In general, it was found that O4 AMF at 477 nm is more sensitive to APH than that at 340 nm and is stably retrieved with low spectral fitting error in Differential Optical Absorption Spectroscopy (DOAS) analysis. In high AOD condition, sensitivity of O4 AMF on APH tends to increase. O4 AMF at 340 nm decreased with increasing solar zenith angle. This dependency isthought to be induced by the decrease in length of the light path where O4 absorption occurs due to the shielding effect caused by Rayleigh and Mie scattering at high solar zenith angles above 40°. At 477 nm, as the solar zenith angle increased, multiple scattering caused by Rayleigh and Mie scattering partly leads to the increase of O4 AMF in nonlinear function. Based on synthetic radiance, APHs have been retrieved using O4 AMF. Additionally, the effect of AOD uncertainty on APH retrieval error has been investigated. Among three aerosol types, APH retrieval for sulfate type is found to have the largest APH retrieval error due to uncertainty of AOD. In the case of dust aerosol, it was found that the influence of AOD uncertainty is negligible. It indicates that aerosol types affect APH retrieval error since absorption scattering characteristics of each aerosol type are various.

Comparison of Germination Characteristics and Daily Seed Germinating Pattern in Fine-textured Fescues (세엽형 훼스큐속 잔디의 발아특성 및 일일 발아패턴 비교)

  • Kim, Kyoung-Nam;Park, So-Hyang
    • Horticultural Science & Technology
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    • v.28 no.4
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    • pp.567-573
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    • 2010
  • Research was initiated to investigate early establishment characteristics and germination pattern of fine-textured fescues (FF). Six varieties from Chewings fescue ($Festuca$ $rubra$ L. ssp. $commutata$ Gaud., CF), creeping red fescue ($F.$ $rubra$ L. ssp. $rubra$ Gaud., CRF), hard fescue ($F.$ $ovina$ ssp. $longifolia$ Thuill., HF) and sheep fescue ($F.$ $ovina$ L., SF) were evaluated in the study. An alternative environmental condition requiring a FF germination test by International Seed Testing Association (ISTA) was applied in the experiment, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$ (ISTA conditions). Daily and cumulative germination patterns were measured and analyzed on a daily basis. Significant differences were observed in germination pattern, days to the first germination, days to 50% germination, days to 60% germination, and germination rate. The final germination percentage was variable with species and varieties, being 40.25 to 82.00% at the end of study. There were considerable variations in early germination characteristics and germination pattern among FF species. The first germination in all entries except HF was initiated between 5 and 6 DAS (days after seeding) under ISTA conditions, while HF between 6 and 7 DAS, being 1 day later. It was 8 to 10 DAS in days to the 50% germination, which was 2 to 4 days after the first germination date. Days to the 60% germination were 9.10 to 14.80 DAS under ISTA conditions, being 5.70 days in differences among the entries. CF 'Jamestown II' and 'Shadow II' and HF 'Aurora Gold' were the fast varieties. The slowest one was HF 'Rescue 911'. Among FF species, turf establishment speed was becoming faster in CRF, SF, HF and CF in this order. Information on differences in germination characteristics and pattern from this study would be usefully applied for golf course design and construction, when established with FF.

Relationship between Light Environment and Crop Growth under Various Nitrogen Application Rates Condition in Rice Plant Canopy (질소(窒素) 시비수준(施肥水準)에 따른 벼 군락내(群落內) 광환경변화(光環境變化)와 생육(生育)과의 관계(關係)에 관(關)한 연구(硏究))

  • Lee, Jeong-Taek;Kazuo, Kobayashi
    • Korean Journal of Environmental Agriculture
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    • v.8 no.2
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    • pp.128-135
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    • 1989
  • To find the effects of solar energy into the rice canopy and its balance on the rice plant growth, a Tongil type rice, Raekyeong and a japonica rice, Koganebare were used with four levels of nitrogen fertilizer application, 6, 10, 14, and 18kg $l0a^{-1}$ in Chigugo, Japan. The micrometerosological data, the solar radiation and absorbed solar radiation by the rice plants, and leaf area index on cardinal growth stage of the rice community were measured. The results are as follows : Raekyeong showed increased LAI by increased nitrogen fertilizer application rates, and larger LAI than Koganebear. 1. There was no difference in total dry weight till 20 days after transplanting regardless of nitrogen levels in the same variety, after that, however, Raekyeong showed higher dry matter productions for the same durations than Koganebare. 2. In early growth stage of transplanting rice, reflection ratio of solar radiation above the crop canogy was about 6%, however, it was increased up to 20% by the increased LAI at heading date. 3. In high levels of nitrogen application plots, LAI were increased so that values were decreased. 4. Relationship between the amount of absorbed radiation by plants and its dry matter production was linearly significant. Higher levels of nitrogen application produced higher dry matter in Raekyeong, however, in lower level, the dry matter production pattern was almost similar between both rice cultivars.

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Study of Absorbed Dose and Effective Dose for Prostate Cancer Image Guided Radiation Therapy using kV Cone Beam Computed Tomography (kV Cone Beam Computed Tomography (CBCT)를 이용한 전립선암 영상유도방사선치료 시 흡수선량 및 유효선량에 관한 고찰)

  • Na, Jong-Eok;Lee, Do-Geun;Kim, Jin-Soo;Baek, Geum-Mun;Kwon, Kyung-Tae
    • The Journal of Korean Society for Radiation Therapy
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    • v.21 no.2
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    • pp.67-74
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    • 2009
  • Purpose: To evaluate the results of absorbed and effective doses using two different modes, standard mode (A-mode) and low-dose mode (B-mode) settings for prostate cancer IGRT from CBCT. Materials and Methods: This experimental study was obtained using Clinac iX integrated with On Board Imager (OBI) System and CBCT. CT images were obtained using a GE Light Speed scanner. Absorbed dose to organs from ICRP recommendations and effective doses to body was performed using A-mode and B-mode CBCT. Measurements were performed using a Anderson rando phantom with TLD-100 (Thermoluminescent dosimeters). TLD-100 were widely used to estimate absorbed dose and effective dose from CBCT with TLD System 4000 HAWSHAW. TLD-100 were calibrated to know sensitivity values using photon beam. The measurements were repeated three times for prostate center. Then, Evaluations of effective dose and absorbed dose were performed among the A-mode and B-mode CBCT. Results: The prostate absorbed dose from A-mode and B mode CBCT were 5.5 cGy 1.1 cGy per scan. Respectively Effective doses to body from A mode and B-mode CBCT were 19.1 mSv, 4.4 mSv per scan. Effective dose from A-mode CBCT were approximately 4 times lower than B-mode CBCT. Conclusion: We have shown that it is possible to reduce the effective dose considerably by low dose mode(B-mode) or lower mAs CBCT settings for prostate cancer IGRT. Therefore, we should try to select B-mode or low condition setting to decrease extra patient dose during the IGRT for prostate cancer as possible.

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Evaluation of Viral Inactivation Efficacy of a Continuous Flow Ultraviolet-C Reactor (UVivatec) (연속 유동 Ultraviolet-C 반응기(UVivatec)의 바이러스 불활화 효과 평가)

  • Bae, Jung-Eun;Jeong, Eun-Kyo;Lee, Jae-Il;Lee, Jeong-Im;Kim, In-Seop;Kim, Jong-Su
    • Microbiology and Biotechnology Letters
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    • v.37 no.4
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    • pp.377-382
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    • 2009
  • Viral safety is an important prerequisite for clinical preparations of all biopharmaceuticals derived from plasma, cell lines, or tissues of human or animal origin. To ensure the safety, implementation of multiple viral clearance (inactivation and/or removal) steps has been highly recommended for manufacturing of biopharmaceuticals. Of the possible viral clearance strategies, Ultraviolet-C (UVC) irradiation has been known as an effective viral inactivating method. However it has been dismissed by biopharmaceutical industry as a result of the potential for protein damage and the difficulty in delivering uniform doses. Recently a continuous flow UVC reactor (UVivatec) was developed to provide highly efficient mixing and maximize virus exposure to the UV light. In order to investigate the effectiveness of UVivatec to inactivate viruses without causing significant protein damage, the feasibility of the UVC irradiation process was studied with a commercial therapeutic protein. Recovery yield in the optimized condition of $3,000\;J/m^2$ irradiation was more than 98%. The efficacy and robustness of the UVC reactor was evaluated with regard to the inactivation of human immunodeficiency virus (HIV), hepatitis A virus (HAV), bovine herpes virus (BHV), bovine viral diarrhea virus (BVDV), porcine parvovirus (PPV), bovine parvovirus (BPV), minute virus of mice (MVM), reovirus type 3 (REO), and bovine parainfluenza virus type 3 (BPIV). Non enveloped viruses (HAV, PPV, BPV, MVM, and REO) were completely inactivated to undetectable levels by $3,000\;J/m^2$ irradiation. Enveloped viruses such as HIV, BVDV, and BPIV were completely inactivated to undetectable levels. However BHV was incompletely inactivated with slight residual infectivity remaining even after $3,000\;J/m^2$ irradiation. The log reduction factors achieved by UVC irradiation were ${\geq}3.89$ for HIV, ${\geq}5.27$ for HAV, 5.29 for BHV, ${\geq}5.96$ for BVDV, ${\geq}4.37$ for PPV, ${\geq}3.55$ for BPV, ${\geq}3.51$ for MVM, ${\geq}4.20$ for REO, and ${\geq}4.15$ for BPIV. These results indicate that UVC irradiation using UVivatec was very effective and robust in inactivating all the viruses tested.

Effect of Planting Density on the Growth and Yield in Staking Cultivation of Bitter Gourd (Momordica charantia L.) under Non-heated Greenhouse (여주 무가온 하우스내 입체재배시 재식밀도가 생육 및 수량에 미치는 영향)

  • Seong, Ki-cheol;Kim, Chun Hwan;Wei, Seung Hwan;Lim, Chan Gyu;Son, Danial
    • Journal of Bio-Environment Control
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    • v.24 no.3
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    • pp.173-177
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    • 2015
  • This experiment was conducted to determined the optimum planting density for the production of high quality bitter gourd (Momordica charantia L.) adapted in spring cultivation with the unheated greenhouse condition. 'Erave' variety was planted at three different planting densities (235, 305, $380plants{\cdot}10a^{-1}$) on March 26. The training method was six lateral vines with pinching the main one. The light intensity was lower in the higher planting density than the lower one. Net photosynthetic rates of the bitter gourd leaves in the higher density were significantly lower (41 to 71%) than the lower one. There was no difference in the fruit characteristics among treatments. But the root weight was heavier in the lower planting density ($235plants{\cdot}10a^{-1}$) as 113.1g than 96.0g of the higher planting density ($380plants{\cdot}10a^{-1}$). The number of the harvested fruit also higher in the lower planting density ($235plants{\cdot}10a^{-1}$) with 60.7 than 39.9 of the higher planting density ($380plants{\cdot}10a^{-1}$). The average fruit weight was the highest in the plot of $305plants{\cdot}10a^{-1}$ as 338.7g and lowest in the lower planting density ($235plants{\cdot}10a^{-1}$) as 285.2g. The total yield of $305plants{\cdot}10a^{-1}$ density was $5,359kg{\cdot}10a^{-1}$, which was higher than $4,068kg{\cdot}10a^{-1}$ of the lower planting density ($235plants{\cdot}10a^{-1}$). Marketable yield was increased by 24% in the planting density of $305plants{\cdot}10a^{-1}$($4,767kg{\cdot}10a^{-1}$) as compared to the lower density in $235plants{\cdot}10a^{-1}$($3,629kg{\cdot}10a^{-1}$) and increased by 13% in the planting density as $380plants{\cdot}10a^{-1}$($4,137kg{\cdot}10a^{-1}$). Therefore, the planting density of bitter gourd was desirable in $305plants{\cdot}10a^{-1}$ density for the higher yield and quality in the protected cultivation.

Apo E4 Genotype as the Alzheimer Indictor in Korean Senior Subjects Aged 50 to 64 Years Old (남녀 노인층에서 Alzheimer위험 예측 인자로써의 Apo E4 Genotype에 관한 연구)

  • Oh, Hyun-Hee;Shin, Eun-Jung;Kim, Hyun-Sook;Lim, Yun-Sook;Park, Mi-Young;Kim, Kkoch-Byul;Kim, Eun-Mee;Lee, Myoung-Sook
    • Journal of Nutrition and Health
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    • v.40 no.7
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    • pp.593-600
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    • 2007
  • Recent studies described the ${\varepsilon}4$ allele of apoE confers a two-to fourfold increased risk for late-onset Alzheimer#s disease (LOAD), but LOAD pathology does not all fit neatly around apo E. Therefore, the goal of this study was to find the association between Alzheimer and apo E4 genotype in the 107 elderly between 50 to 64 years old who visited to FHWC of Sungshin Women#s University. We conducted the questionnaire survey (general & 24 hr dietary recall), anthropometerics (BP, waist & BMI) and blood biochemistry (FBS & lipid profiles). LDL-c and HOMA-IR were calculated by Friedwald#s and Matthew#s formulas. The apo E genotyping was performed by PCR-RFLP method and subjects were divided into three allele groups (${\varepsilon}3$; wild, ${\varepsilon}2$ & ${\varepsilon}4;$ mutants). The apo E allele frequencies were 7.0% for the ${\varepsilon}2$, 83.6% for the ${\varepsilon}3$ and 9.3% for the ${\varepsilon}4$. In comparison with biochemistry characteristics by apo E genotype, FBS was significantly higher in ${\varepsilon}4(129.2{\pm}6.8)$ than that in the others (${\varepsilon}2$: $117{\pm}7.4$, ${\varepsilon}3$: $107.3{\pm}2.2)$ (p<0.01). More than forty percents of ${\varepsilon}4$ group shown the dyslipidemia [high TG (>150mg/dl) & low HDL (<40 mg/dl:male or <50 mg/dl: female)]. The cytokines levels such as IL-1 ${\beta}$, IL-6 and $TNF-{\alpha}$ were not different among three apoE alleles. After the adjusting sex, age & dietary fiber, LDL-c level was siginificantly higher in ${\varepsilon}4$ ($108.3{\pm}7.7$) than that in ${\varepsilon}2$ ($100.4{\pm}8.4$) (p<0.05). According to food intake and the recipe on the basis of 24 hr dietary recall, the elder]y with ${\varepsilon}4$ allele took higher intake frequency of the light -colored vegetable (radish, onion & cabbage) and pan-fried foods (sauteed beef and vegetables, stir-fried vienna with vegetables) than the others. We knew that the elderly with ${\varepsilon}4$ allele had been restricted the calories intakes with high dietary fiber (33.6+2.5 g/d) to maintain the normal level of FBS and LDL-c. On next study, the prevalence of Alzheimer#s disease in this population who has ${\varepsilon}4$ allele on the condition of calories restriction will be continually follow-up.

The effect of silane treatment timing and saliva contamination on shear bond strength of resin cement to porcelain (Silane의 처리시기와 타액오염이 도재-레진 시멘트의 전단 결합강도에 미치는 영향)

  • Ro, Young-Seon;Ryu, Jae-Jun;Suh, Kyu-Won
    • The Journal of Korean Academy of Prosthodontics
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    • v.47 no.1
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    • pp.61-69
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    • 2009
  • Statement of problem: Porcelain veneers have become a popular treatment modality for aesthetic anterior prosthesis. Fitting porcelain veneers in the mouth usually involve a try-in appointment, which frequently results in salivary contamination of fitting surfaces. Purpose: An in vitro study was carried out to investigate the effect of silane treatment timing and saliva contamination on the resin bond strength to porcelain veneer surface. Material and methods: Cylindrical test specimens (n=360) and rectangular test specimens (n=5) were prepared for shear bond test and contact angle analysis. Whole cylindrical specimens divided into 20 groups, each of which received a different surface treatment and/or storage condition. The composite resin cement stubs were light-polymerized onto porcelain adherends. The shear bond strengths of cemented stubs were measured after dry storage and thermocycling (3,000 cycles) between 5 and $55^{\circ}C$. The silane and their reactions were chemically monitored by using Fourier Transform Infrared Spectroscopy analysis (FTIR) and contact angle analysis. One-way analysis of variance (ANOVA) and Dunnett's multiple comparison were used to analyze the data. Results: FT-IR analysis showed that salivary contamination and silane treatment timing did not affect the surface interactions of silane. Observed water contact angles were lower on the saliva contaminated porcelain surface and the addition of 37% phosphoric acid for 20 seconds on saliva contaminated porcelain increased the degree of contact angle. Silane applied to the porcelain, a few days before cementation, resulted in increasing the bond strength after thermocycling. Conclusion: Within the limitation of this study, it can be concluded that it would be better to protect porcelain prosthesis before saliva contamination with silane treatment and to clean the contaminated surface by use of phosphoric acid.