• 미생물과산업
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• 제14권1호
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• pp.17-20
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• 1988

A genetic analysis of the complex Bacillus subtilis transcriptional apparatus is essential to understand the function, regulation, and interaction of the transcriptase components during growth and sporulation. This approach in Escherichia coli has uncovered fundamental mechanisms regulating gene expression Cole and Nomura, 1986; Lindahl and Zengel, 1986) and an analysis of the B. subtilis transcriptase will allow comoparison of the E.coli system to another bacterium that has evolved under different selective pressures. To this end we used antibody probes to isolate the alpha, beta, and beta' core subunit genes from a .lambda.gtill expression vector library. To address the question of function ans regulation of the minor sigma factors that confer promoter specifity on the polymerase core (Losick et al., 1986), we used the same approach to isolate the gene for the 37,000 dalton sigma factor, sigma-37.

• 한국환경성돌연변이발암원학회지
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• 제19권1호
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• pp.28-33
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• 1999

The SNF2 family includes proteins from a variety of species with roles I cellular processes such as transcriptional regulation, recombination and various types of DNA repair. Several proteins with unknown function are also included in this family. Here, we report the cloning and characterization of hrp 2+ gene (helicase related gene from S. pombe) which was isolated by PCR amplication using the conserved domain of SNF2 motifs within the ERCC6 gene which encodes a protein involved in DNA excision repair. The hrp2+ gene was isolated by screening with yeast S. pombe genomic library. The isolated cloned contained 6.5 kb insert DNA. Southern blot analysis confirmed that S. pombe chromosome contains the same DNA as hrp2+ gene and this gene exists as a single copy in S. pombe genome. The 4.7 kb transcript of mRNA was identified by Northern blot. To examined the transcriptional regulation of hrp2+ gene, DNA damaging agents were treated. These results indicated that the hrp2+ gene may not be directly involved in DNA replication, but may be involved in damage response pathway.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.93.3-94
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• 2003

The signaling components of high affinity IgE receptor (Fc RI) were searched by yeast-hybrid screening of the cDNA library constructed from RBL-2H3 cells. The cytoplasmic part of the Fc RI- chain was found to specifically interact with PLC 2, and further comparatives studies were conducted focusing on the differential regulation of two PLC- isoforms through Fc RI. The inhibitors of Src, Syk, and protein kinase C similarly affected the tyrosine phosporylations of PLC 1 and PLC 2 but the inhibitors of PI3-kinase and p42/44 ERK effectively inhibited the activation of PLC 1 but not PLC 2. (omitted)

• 한국도서관정보학회지
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• 제25권
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• pp.225-256
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• 1996

The purpose of this study is to find the ways that will be helpful to improve archival system and to establish National Archives. The contents of this study were focused on comparing the characteristics of archival system in governmental administration,: Executive, Legislature, and Judiciary. They were also focused on analyzing the problems of those current system. This research was basically conducted depending on the detailed articles of the legislation and regulation pertinent to the archives. Two major results of the research are 1) There are much differences among the governmental administrations in structuring and organizing for archival administrations. Archival works of government document are divided primarily according to the period of conservation and it is necessary to establish the regional archives and central management system, and to employ archivists as an expert staff. 2) The principles and methods of archival process, such as transferences, classifications, preservations, access, and destructions are similar to each other. In order to improve and co-ordinate current systems, it is necessary to constitute several councils endowed with consultative and decision-making power.

• 한국도서관정보학회지
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• 제36권2호
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• pp.141-163
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• 2005

최근에 들어와서 참고문헌이 정보원으로서의 가치가 높아짐에 따라 참고문헌 데이터베이스 구축이 증가하고 있다. 그러나 각 학술지에서 제시하는 참고문헌의 기술규정이 서로 다르고 연구자들마다 참고문헌의 기술규정을 올바르게 인지하지 못한 채로 참고문헌을 다양한 형식으로 기재하고 있다. 이러한 요인들은 참고문헌 데이터베이스의 효과적인 구축을 어렵게 하는 요인이 되고 여다. 따라서 본 연구는 참고문헌 기술규정의 상이성을 면밀히 분석하여 서지기술상의 문제점을 밝히고, 이를 토대로 참고문헌 기술규정 통일화를 위한 방안을 제시하고자 한다.

• 한국도서관정보학회지
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• 제47권2호
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• pp.231-257
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• 2016

이 연구는 2014년 "출판문화산업진흥법"의 개정으로 새로운 내용의 도서정가제가 시행됨에 따라 공공도서관 자료구입과정에서 쟁점으로 부각되고 있는 문제를 짚어 본 다음, 이를 토대로 개정된 도서정가제의 취지를 살림과 동시에 보다 효율적인 자료구입이 이루어지기 위한 과제를 제안하고자 하였다. 연구를 통해 도출된 결과는 다음과 같다. 첫째, 개정 도서정가제 시행으로 가격경쟁 비중이 축소된 상황에서 도서관은 업무이행능력 등 질적인 측면에 초점을 맞추어 자료납품업체를 선정할 수 있는 방안을 마련해야 한다는 점 둘째, 자료납품업체로서 지역서점의 개념과 범위를 명확하게 설정하여 무자격업체의 응찰을 방지함과 동시에 지역서점 활성화 정책의 취지를 살려야 한다는 점 셋째, 경제상의 이익 처리에 대한 보다 명확한 규정이 마련되어야 한다는 점 등이 그것이다.

• 한국문헌정보학회지
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• 제26권
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• pp.113-139
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• 1994

Government archives are basic source materials in the historical study. Whereas, researches on the archives of Choson-chongdokbu have been very scant. Those archives contain information about both colonism of Japan and independent movement of Korea are comparatively well preserved at Government Archives and Record Services. Therefore, it is necessary to analyze the characteristic of archives and to evaluate the system of archival management of Choson-chongdokbu. Based on the official gazette and archives of Choson-chongdokbu, this study has revealed the division of archival work, the writing and treatment of official document, compilation and preservation, and the procedure of use and destruction. As a result of the research, the followings have been identified; 1. Archival regulations were prepared by the instructions of Choson­chongdokbu at the early days of its establishment. Inspite of several revisions, its basis was maintained until 1945. 2. Each agency had its own archival department instead of central archives. 3. The archival department of Choson-chongdokbu controlled the archival administration of headquarters and attached agencies. But the secret archives were managed by the secretarial department. 4. The writing and treatment of archives were done by the archival regulation made in 1911. 5. Each agency had set more or less different preservation period. 6. There were three ways of using archives, 1) the in-house reading 2) the loan 3) the transcriptions. The archives passed the preservation period were destroyed in accordance with the related departments.

• 인지과학
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• 제1권2호
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• pp.361-376
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• 1989

카테콜아민 생합성에 관여하는 마지막 효소인 phenylethanolamine Nmethyltransferase는 norepinephrine 을 epinephrine으로 전환시키는 중요한 효소이다. PNMT효소의 발현은 epinephrine 신경세표의 발현에 필수적이다.따라서 PNMT 유전자를 크로닝하여 그 구조를 결정하고,유전자 발현연구를 하는 것은 상당히 중요한 일이다.그러나 최근에 저자가 bovine 및 human cDA 를 처음으로 분리하여 그 구조를 보고한 것 외에는 아직까지 인간과 백서 전체 genomic DNA 의 분리 보고는 없다.이에 저자들은 인간과 백서 PNMT유전자의 전체구조와 여러종(species)사이의 진화적인 관계를 규명하기 위해서 human 과 Rat genomic library 를 만들고,이 library 를 이용하여 bovine cDNA 를 probe로 13.1kb와 13.2kb길이의 인간과 백서의 genomic clone 을 분리 크리닝하는데 성공하여 유전자의 구조적 규명하였다.

• Biomolecules & Therapeutics
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• 제31권1호
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• pp.48-58
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• 2023

Interferon regulatory factor 3 (IRF3) integrates both immunological and non-immunological inputs to control cell survival and death. Small GTPases are versatile functional switches that lie on the very upstream in signal transduction pathways, of which duration of activation is very transient. The large number of homologous proteins and the requirement for site-directed mutagenesis have hindered attempts to investigate the link between small GTPases and IRF3. Here, we constructed a constitutively active mutant expression library for small GTPase expression using Gibson assembly cloning. Small-scale screening identified multiple GTPases capable of promoting IRF3 phosphorylation. Intriguingly, 27 of 152 GTPases, including ARF1, RHEB, RHEBL1, and RAN, were found to increase IRF3 phosphorylation. Unbiased screening enabled us to investigate the sequence-activity relationship between the GTPases and IRF3. We found that the regulation of IRF3 by small GTPases was dependent on TBK1. Our work reveals the significant contribution of GTPases in IRF3 signaling and the potential role of IRF3 in GTPase function, providing a novel therapeutic approach against diseases with GTPase overexpression or active mutations, such as cancer.

• 한국동물학회:학술대회논문집
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• 한국동물학회 2003년도 한국생물과학협회 학술발표대회
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• pp.200.3-200
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• 2003

No Abstract, See Full Text