• Asian-Australasian Journal of Animal Sciences
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• v.31 no.5
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• pp.650-657
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• 2018

Objective: The study investigated the biological functions and mechanisms for controlling cashmere growth of Liaoning cashmere goat by ovarian carcinoma immunoreactive antigen-like protein 2 (OCIAD2) and decorin (DCN) genes. Methods: cDNA library of Liaoning cashmere goat was constructed in early stages. OCIAD2 and DCN genes related to cashmere growth were identified by homology analysis comparison. The expression location of OCIAD2 and DCN genes in primary and secondary hair follicles (SF) was performed using in situ hybridization. The expression of OCIAD2 and DCN genes in primary and SF was performed using real-time polymerase chain reaction (PCR). Results: In situ hybridization revealed that OCIAD2 and DCN were expressed in the inner root sheath of Liaoning cashmere goat hair follicles. Real-time quantitative PCR showed that these genes were highly expressed in SF during anagen, while these genes were highly expressed in primary hair follicle in catagen phase. Melatonin (MT) inhibited the expression of OCIAD2 and promoted the expression of DCN. Insulin-like growth factors-1 (IGF-1) inhibited the expression of OCIAD2 and DCN, while fibroblast growth factors 5 (FGF5) promoted the expression of these genes. MT and IGF-1 promoted OCIAD2 synergistically, while MT and FGF5 inhibited the genes simultaneously. MT+IGF-1/MT+FGF5 inhibited DCN gene. RNAi technology showed that OCIAD2 expression was promoted, while that of DCN was inhibited. Conclusion: Activation of bone morphogenetic protein (BMP) signaling pathway up-regulated OCIAD2 expression and stimulated SF to control cell proliferation. DCN gene affected hair follicle morphogenesis and periodic changes by promoting transforming growth $factor-{\beta}$ ($TGF-{\beta}$) and BMP signaling pathways. OCIAD2 and DCN genes have opposite effects on $TGF-{\beta}$ signaling pathway and inhibit each other to affect the hair growth.

• Journal of the Korean Society for information Management
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• v.34 no.3
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• pp.7-21
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• 2017

This study analyzed the methods for representing and linking personal information in the linked data of National Library of Korea and provided suggestions for expanding the scope of identifying and linking of the personal information. As a result, the personal information as a subject has been dealt with a concept, where the personal information as a contributor has been linked with a vocabulary of personal name. In addition, there have not been assured of including additional information except existing authority data in the process of building the linked data. Therefore, this study suggested that linking personal information as a subject and personal information as a contributor was essential for the quality of linked data. In addition, we proposed to provide additional information related to the person in linked data for expanding the scope of access points in information discovery.

• Journal of Life Science
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• v.25 no.6
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• pp.656-662
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• 2015

Natural products and natural product structures play a general and highly significant role in drug discovery and development process because it has various merits and potentials for new drug source that have extensive clinical experience, development time contraction, excellent stability and safety. In several neurological disorders, neuronal death and excessive activation of microglia (neuro-inflammation) are observed. A number of drug discovery-related neuronal cell death and neuro-inflammation was studied from natural products, respectively. However, until now, it has not been possible to study dual-protection molecules recorded in the Natural Product library. In the present study, using the natural product-derived library of the Institute for Korea Traditional Medical Industry, we investigated dual-protective molecules against glutamate (a classical excitatory neurotransmitter)-induced oxidative stress mediated neuronal cell death and LPS-induced excessive activated microglial cells (immune cells of the brain). Chrysophanol, extracted from Rheum palmatum, had dual-protective effects against both glutamate-induced neuronal cell death and LPS-induced NO production, triggering proinflammatory cytokines and microglia activation and resulting in neuroinflammation. Flow-cytometry analysis revealed that chrysophanol had a scavenger effect, scavenging glutamate- and LPS-induced reactive oxygen species (ROS) produced by neuronal and microglial cells, respectively. Based on the present study, chrysophanol may have an important protective role against neuronal cell death and neuroinflammation in the brain. The results may be helpful for studying drug development candidates for treating central nervous system disorders.

• Journal of the Korean Society for Library and Information Science
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• v.56 no.4
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• pp.285-307
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• 2022

This study examines the policies currently announced in the "3rd Comprehensive Library Development Plan" in relation to the discovery of individuals' potential in libraries and analyzes the current status of the tasks promoted and executed by central administrative agencies and municipal and provincial libraries based on these policies. Based on the analysis results, this study presents the methods for libraries to play their role in discovering the potential of individuals in the future. According to the results of this study, due to the poor performance of libraries resulting from reduced in-person events because of library closures and the promotion of non-contact services such as humanities classes and cultural programs in response to COVID-19, it is necessary to establish systems to support emergency management and non-contact programs in response to COVID-19, as well as increase the budget for extending reading culture projects for underprivileged regions and people. In addition, it is necessary to discover quality long-term, non-contact programs, provide infrastructure for non-contact program operation, and develop guidelines. Second, it is observed that communication- and discussion-type social reading programs need to be promptly converted into and operated as online classes. Third, the study results confirm that it is necessary to prepare measures to reinforce external cooperation, such as improving the convenience of and accessibility to information use for users, conducting seminars between domestic and foreign councils, promoting one-stop data sharing systems, executing data digitalization, expanding library infrastructure in preparation for the post-COVID-19 era, and promoting public relations activities.

• Journal of Korean Library and Information Science Society
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• v.30 no.4
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• pp.255-278
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• 1999

The Korean oldbooks that represent the spiritual product of the ancestors in the form of physical shape are absolute need for retrieval and new evaluation through research and discovery. To earn Korean oldbooks that have these values, catalogue of Korean oldbooks and annotation book were used as secondery materials. But since there haven't been publications of Korean oldbook catalogue that can be used as Korean oldbook search engine, Korean oldbooks that has same title was valued differently by the different authors and defined differently according to bibliographic study. And there even has been a case when, even though there is same edition it was consider the only one. Therefor it can cause a lot of mistakes when you can't over look totally. So you can expert better results in structuring DB with computer then publicized catalogue of Korean oldbooks. But at this present time, since all of the DB that have been structured are not unified as one system and operated separately in their organizations, there has been a problem like creating different bibliographic information for same Korean oldbooks. In this study, as one of the solution to the problems of searching Korean oldbooks, we are making a plan to develop the unified retrieval system of Korean oldbooks using hypertext system. And with these finding, we are suggesting the further way of developing retrieval system of Korean oldbooks.

• Journal of Korean Library and Information Science Society
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• v.46 no.4
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• pp.325-354
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• 2015

Corporate information has great value, but also poses great risk. Information governance is an organization's coordinated, inter-disciplinary approach to satisfying information compliance requirements and managing information risks while optimizing information value. This study aims to suggest the directions of implementing information governance in business firms based on analyses of Sedona Conference Principles of Information Governance. After analysing and reformulating the principles into control standards, and they are investigated and interpreted in terms of practicing information governance.

• Journal of Korean Library and Information Science Society
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• v.41 no.3
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• pp.269-290
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• 2010

Applying one specific thesaurus might cause several problems because each thesaurus has its own characteristics inherited from its construction process. Therefore, integration of thesauri can be an appropriate approach to overcome the difficulties. This current research selected physics as a domain and two thesauri in the domain: PACS and PIRA. By integrating these two heterogeneous thesauri, this research could construct a conceptual structure that covers the whole concepts related to physics. By constructing the conceptual structure with the use of facet analysis from integrated thesaurus, it provides knowledge base with hierarchical structure and clear relationships between concepts. It can be an alternate approach to effective and efficient information retrieval and knowledge discovery.

• Journal of Microbiology and Biotechnology
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• v.23 no.6
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• pp.779-784
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• 2013

Leptospirosis is a worldwide zoonosis of global concern caused by Leptospira interrogans. The availability of ligand libraries has facilitated the search for novel drug targets using chemogenomics approaches, compared with the traditional method of drug discovery, which is time consuming and yields few leads with little intracellular information for guiding target selection. Recent subtractive genomics studies have revealed the putative drug targets in peptidoglycan biosynthesis pathways in Leptospira interrogans. Aligand library for the murD ligase enzyme in the peptidoglycan pathway has also been identified. Our approach in this research involves screening of the pre-existing ligand library of murD with related protein family members in the putative drug target assembly in the peptidoglycan biosynthesis pathway. A chemogenomics approach has been implemented here, which involves screening of known ligands of a protein family having analogous domain architecture for identification of leads for existing druggable protein family members. By means of this approach, one murC and one murF inhibitor were identified, providing a platform for developing an anti-leptospirosis drug targeting the peptidoglycan biosynthesis pathway. Given that the peptidoglycan biosynthesis pathway is exclusive to bacteria, the in silico identified mur ligase inhibitors are expected to be broad-spectrum Gram-negative inhibitors if synthesized and tested in in vitro and in vivo assays.

• Journal of the Korean Society for Library and Information Science
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• v.43 no.1
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• pp.189-214
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• 2009

The study acknowledges the values of periodical indexing as information resources. The study identified periodicals users' needs of article indexing services based on focus group interviews. The study also conducted a comparative study of periodicals indexing services of libraries and databases in Korea and the US. The study argues for the need of seamless services for users of periodical articles indexing services. The study also recommends the elements needed for improving the current service, which includes establishing a collaborative indexing system, adopting a metadata standard, implementing authority files, incorporating social web services, offering diverse ways of information discovery based on facet approach, and stabilizing identification systems.

• Korean Journal of Plant Resources
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• v.20 no.6
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• pp.491-498
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• 2007

It has been hypothesized that efficient exclusion of methylated retrotransposons and repeated DNA region is one of the rapid and cost-effective approaches for comprehensive gene discovery in large genome size of maize. Three kinds of methylation-sensitive restriction enzymes, HapII, MspI and McrBC, were used to identify the restriction frequency of cytosine methylation sites in maize genome. Roughly 60% of total maize genomic DNA was restricted less than 500bp by McrBC, and the most of restricted small size fraction was composed retrotransposon. In order to validate the efficient construction of gene-rich shotgun library, we compare two gene-rich methyl-filtration shotgun libraries using in vivo and in vitro methyl-filtration system. The size selected DNA fraction by Sau3A-McrBC enzyme treated was very stable and has not appeared modification in E. coli, but most insert DNA size of partially digested with Sau3A were decrease less than 500bp by bacterial methylation-modification system. In compare of retroelements portion, A 44.6% of the sequences were retroelement in unmethyl-filtered library, and the most of them was Copia type, such as Prem, Opie and Ji. The portion of retroelement was drastically decreased to 25% and 20% by in vivo and in vitro filtration system, respectively.