• Fisheries and Aquatic Sciences
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• 제6권3호
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• pp.110-115
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• 2003

Expressed sequence tag (EST) analysis was conducted using a cDNA library made from the liver mRNA of olive flounder (Paralichthys olivaceus). In the survey of 421 ESTs, 362 showed significant homology to previously described genes while 59 were unidentified or novel. Comparative analysis of the identified ESTs showed that 69 $(19.0\%)$ clones were identified as homologous to the previously reported olive flounder ESTs, and 279 $(77.1\%)$ clones were identified as orthologs of known genes from other organisms. The remaining 14 $(3.9\%)$ clones were identified as orthologs of known sequences with unknown functions. These tagged cDNA clones, identified and unidentified, could provide fundamental baseline data for genomic studies of this species.

• Journal of Microbiology and Biotechnology
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• 제12권5호
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• pp.716-721
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• 2002

The ldhL gene encoding the $_L$-(+) lactate dehydrogenase was cloned from Lactobacillus reuteri ATCC 55739 chromosomal DNA and characterized. An internal 750-bp fiagment of ldhL gene was amplified by PCR using primers based on the conserved region of lactobacilli ldhL genes. A genomic library off. reuteri ATCC 55739 was constructed using pBR322, and colony hybridization experiments were performed using the 750-bp fragment as aprobe. One clone harboring a 4.0-kb PstI fragment was identified, and nucleotide sequencing confirmed it as an open reading frame of 972 bp in size in the middle. In addition to IdhL gene, an ORF homologous to Streptococcus pneumoniae TIGR4 hydrolase gene and 3' part of phosphomevalonate kinase gene (mvaK2) were also found on the 4 kb fragment. $_L$-LDH of L. reuteri ATCC 55739 showed the highest degree of homology with the $_L$-LDH of Pediococcus acidilactici (62.4%), fullowed by the $_L$-LDH of Lactobacillus pentosus (58.7%). The size of IdhL transcript determined by Northern blot was 1 kb, indicating the monocistronic nature of IdhL.

• Parasites, Hosts and Diseases
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• 제41권2호
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• pp.89-96
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• 2003

Among the panel of monoclonal antibodies (mAb) against Toxoplasma gondii, mAb of Tg621 (Tg621) clone blotted 38 kDa protein which localized in the cytoplasm of tachyzoites by immunofluorescence microscopy The protein was not released into the parasitophorous vacuole during or after invasion. The cDNA fragment encoding the protein was obtained by screening a T. gondii cDNA expression library with Tg621. The full length cDNA sequence was completed with 5’-RACE as 1,592 bp, which contained open reading frame of 942 bp. The deduced amino acid sequence of Tg621 consisted of a polypeptide of 313 amino acids, with significant homology to ribosomal P proteins (RPP) of other organisms especially high to those of apicomplexan species. The expressed and purified TgRPP was assayed in western blot with the sera of toxoplasmosis patients and normal sera, which resulted in the 74.0% of positive reactions in toxoplasmosis patients whereas 8.3% in normal group. Therefore, the antibody formation against TgRPP in toxoplasmosis patients was regarded as specific for T. gondii infection and suggested a potential autoantibody.

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권1호
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• pp.38-42
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• 2002

A chitinase gene (pCHi58) encoding a 58 kDa chitinase was isolated from the Serratia marcescens KCTC 2172 cosmid library. The chitinase gene consisted of a 1686 bp open reading frame that encoded 562 amino acids. Escherichia coil harboring the pChi58 gene secreted a 58 kDa chitinase into the culture supernatant. The 58 kDa chitinase was purified using a chitin affinity column and mono-S column. A nucleotide and N-terminal amino acid sequence analysis showed that the 58 kDa chitinase had a leader peptide consisting of 23 amino acids which was cleaved prior to the 24th alanine. The 58 KDa chitinase exhibited a $98\%$ similarity to that of S. marcescens OMB 1466 in its nuclotide sequence. The chitinolytic patterns of the 58 kDa chitinase released N,N'-diacetyl chitobiose (NAG2) as the major hydrolysis end-product with a trace amount of N-acetylglucosamine. When a 4-methylumbellyferyl-N-acetylglucosamin monomer, dimmer, and tetramer were used as substrates, the 58 kDa chitinase did not digest the 4-Mu-NAG monomer $(analogue\;of\;NAG_2)$, thereby indicating that the 58 kDa chitinase was likely an endochitinase. The optimum reaction temperature and pH of the enzyme were $50^{\circ}C$ and 5.0, respectively.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2004년도 ICEIC The International Conference on Electronics Informations and Communications
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• pp.199-203
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• 2004

In this paper, we only describe the digital block of two-channel 18-bit analog-to-digital (A/D) converter employing sigma-delta method and xl28 decimation. The device contains two fourth comb filters with 1-bit input from sigma­delta modulator. each followed by a digital half band FIR(Finite Impulse Response) filters. The external analog sigma-delta modulators are sampled at 6.144MHz and the digital words are output at 48kHz. The fourth-order comb filter has designed 3 types of ways for optimal power consumption and signal-to-noise ratio. The following 3 digital filters are designed with 12tap, 22tap and 116tap to meet the specification. These filters eliminate images of the base band audio signal that exist at multiples of the input sample rate. We also designed these filters with 8bit and 16bit filter coefficient to analysis signal-to-noise ratio and hardware complexity. It also included digital output interface block for I2S serial data protocol, test circuit and internal input vector generator. It is fabricated with 0.35um HYNIX standard CMOS cell library with 3.3V supply voltage and the chip size is 2000um by 2000um. The function and the performance have been verified using Verilog XL logic simulator and Matlab tool.

• 한국통신학회논문지
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• 제38C권9호
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• pp.781-786
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• 2013

Multiplexing information over parallel data channels based on RF MIMO concept is possible to achieve considerable data rates over large transmission ranges with just a single transmitting element. Visual multiplexing MIMO techniques will send independent streams of bits using the multiple elements of the light transmitter array and recording over a group of camera pixels can further enhance the data rates. The proposed system is a combination of the reliance on computer vision algorithms for tracking and OOK cell frame modulation. LED array are controlled to transmit message in the form of digital information using ON-OFF signaling with ON-OFF pulses (ON = bit 1, OFF = bit 0). A camera captures image frames of the array which are then individually processed and sequentially decoded to retrieve data. To demodulated data transmission, a motion tracking algorithm is implemented in OpenCV (Open source Computer Vision library) to classify the transmission pattern. One of the most advantages of proposed architecture is Computer Vision (CV) based image analysis techniques which can be used to spatially separate signals and remove interferences from ambient light. It will be the future challenges and opportunities for mobile communication networking research.

• Nuclear Engineering and Technology
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• 제49권6호
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• pp.1287-1300
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• 2017

Based on the observation that ignoring the angle dependency of multigroup resonance cross sections within a fuel pellet would result in nontrivial underestimation of the spatial self-shielding of flux, a parametrized spectral superhomogenization (SPH) factor library (PSSL) method is developed as a practical means of resolving the problem. Region-wise spectral SPH factors are calculated by the normal and transport corrected SPH iterations after ultrafine group slowing down calculations over various light water reactor pin-cell configurations. The parametrization is done with fuel temperature, U-238 number density, fuel radius, moderator source represented by ${\Sigma}_{mod}V_{mod}$, and the number density ratio of resonance nuclides to that of U-238 in a form of resonance interference correction factors. The parametrization is successful in that the root mean square errors of the interpolated SPH factors over the fuel regions of various pin-cells are within 0.1%. The improvement in reactivity error of the PSSL method is shown to be superior to that by the original SPH method in that the reactivity bias of -200 pcm to -300 pcm vanishes almost completely. It is demonstrated that the environment effect takes only about 4% in the reactivity improvement so that the pin-cell based PSSL method is effective in the assembly problems.

• Nuclear Engineering and Technology
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• 제54권1호
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• pp.11-18
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• 2022

In this research, curium oxide (CmO₂) is studied as fuel for VVER-1200 reactor to get an attention to its energy value and possibilities. For this purpose, CmO₂ is used in fuel rods or integrated burnable absorber (IBA) rods with and without UO₂ and then compared with the conventional fuel assembly of VVER-1200 reactor. It is burned to 60 GWd/t by using SRAC-2006 code and JENDL-4.0 data library. From these studies, it is found that CmO₂ is competent like UO₂ as a fuel due to higher fission cross-section of ²⁴³Cm and ²⁴⁵Cm isotopes and neutron capture cross-section of ²⁴⁴Cm and ²⁴⁶Cm isotopes. As a result, when some or all of the UO₂ of fuel rods or IBA rods are replaced by CmO₂, we get a similar k-inf like the reference even with lower enrichment UO₂ fuels. These studies show that the use of CmO₂ as IBA rods is more effective than the fuel rods considering the initially loaded amount, power peaking factor (PPF), fuel temperature and void coefficient, and the quality of spent fuel. From a detailed study, 3% CmO₂ with inert material ZrO₂ in IBA rods are recommended for the VVER-1200 reactor assembly from the once through concept.

• 대한한방부인과학회지
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• 제34권2호
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• pp.62-81
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• 2021

Objectives: The purpose of this study is to review clinical studies and evaluate effectiveness of intravaginal administration of oriental medicine on vaginal atrophy in menopausal women. Methods: 'postmenopause', 'menopause', 'vaginal atrophy', 'atrophic vaginitis', 'vaginal cream', 'vaginal gel', 'pessary', 'suppository' were searched on 3 online databases (Pubmed, Cochrane Library, OASIS). The risk of bias was assessed by using Cochrane risk of bias (RoB) 2.0 tool. Results: 9 randominized controlled trials (RCTs) were selected among 489 articles. The types of intervention were intravaginal administration of oriental medicine. 6 studies showed that treatment group was better than placebo control group or both treatment group and western medicine control group improved similarly. 2 studies were not significant effective in some results and 1 study was not statistically significant. Conclusions: Intravaginal administration of oriental medicine can be an effective option in treating menopausal women with vaginal atrophy. but considering the small number and low quality of studies, further well-designed studies are needed to confirm the effectiveness of this treatment.

• International Journal of Advanced Culture Technology
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• 제10권2호
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• pp.140-147
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• 2022

This study analyzes research trends by systematically examining research about domestic emergency medical services' clinical practice, and it is aimed to present the basic data needs in development plan in clinical practice education in the future. The thesis was searched through the electronic data research (Science Direct, PubMed, Medline, and 55 academic DB interworking) from the library of Konyang University from 2010 to 2021. The main keywords were "Emergency Medical Technician(EMT) Student" and "Clinical Practice," and 6 pieces of researches were selected, finally. As the result of analyzing the qualitative level of selected research, all the 6 pieces of research subjects(100.0%) were pertinent to level IV(survey research) which is low-qualitative level. As the principal subjects, "Clinical practice experience" got the highest frequency as 6(100%), "Satisfaction of clinical practice" was 3(50%), "Self-efficient, Major satisfaction" and "Stress, Depression, Coping" showed 2 (33.3%), and "Change after clinical practice", "Clinical practice improvement plan" was 1(16.7%) each. From this time on, it is confirmed that the quantitative and qualitative growth in domestic emergency medical clinical practice is necessary. This is expected to contribute to establishing a practical and systematic development plan of clinical practice education.