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Effects of Low Crude Protein Diets Supplemented with Synthetic Amino Acids on Performance, Nutrient Utilization and Carcass Characteristics in Finishing Pigs Reared Using a Phase Feeding Regimen

  • Lee, J.H.;Kim, J.H.;Kim, J.D.;Kim, S.W.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.5
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    • pp.655-667
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    • 2001
  • This experiment was conducted to investigate the effect of feeding a low CP diet supplemented with synthetic amino acids on performance, nutrient utilization and carcass characteristics of finishing pigs fed under a three-phase feeding regimen. Ninety-six finishing pigs (Landrace$\times$Large White$\times$Duroc), $55.75kg{\pm}0.65$ of initial body weight, were blocked by weight and sex and allotted to four dietary treatments in a randomized block design. There were six pens per treatment and four pigs per pen. Pigs were fed a 16%-14%-12% CP (for phase I-II-III, respectively), sequence of diets. Dietary treatments were 1) Control, 2) Con+L (a sequence of diets reduced in CP by l percentage unit with lysine (L) supplementation, 3) Con+LMT (a sequence of diets reduced in CP by 2 percentage unit with LYS, methionine (MET) and threonine (THE) supplementation) and 4) Con+LMTT (a sequence of diets reduced in CP by 3 percentage unit with LYS, MET, THR and tryptophan (TRP) supplementation). The finishing period (55 to 105 kg) was divided into three phases (55 to 72 kg, 72 to 90 kg and 90 to 105 kg). Pigs fed either the control or Con+L diet grew faster (p<0.05) than pigs fed the Con-LMT or Con+LMTT diet. There was no difference in ADFI among dietary treatments. Phosphorus (P) digestibility was lowest in the control group and highest in the Con+LMTT group (p<0.05). Within each phase, no significant differences in dry matter (DM) and CP digestibilities were found. Although some amino acid digestibilities were affected by dietary treatments, digestibilities of essential amino acids (EAA), non-essential amino acids (NEAA) and total amino acid were not significantly influenced by dietary treatments. For the entire experiment periods, Con+L, Con+LMT and Con+LMTT treatments resulted in 13.4, 18.8 and 21.6% lower total N excretion compared with the control. Con+LMT and Con+LMTT treatments showed significantly lower BUN concentration compared with the control and Con+L treatment (p<0.05), but there was no significant difference in BUN concentration between pigs fed the control and Con+L treatment or between pigs fed Con+LMT and Con+LMTT treatments (p>0.05). Carcass length, backfat thickness and carcass grade were not significantly affected by dietary treatments (p>0.05). In conclusion, reducing dietary CP level by 1 percentage unit and supplementing only LYS at each phase could be a very beneficial feeding strategy for finishing pigs fed under a three phase feeding regimen in terms of both environmental and economical aspects.

Utilization of Steam-treated Oil Palm Fronds in Growing Goats: 1. Supplementation with Dietary Urea

  • Paengkoum, Pramote;Liang, J.B.;Jelan, Z.A.;Basery, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.9
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    • pp.1305-1313
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    • 2006
  • Five male dairy goats (Saanen), 4.6 month old with a body weight of 21.4 ($SD{\pm}1.6$) kg, were used to examine 5 dietary urea treatments in a $5{\times}5$ Latin Square experimental design. The five levels of urea were 10, 20, 30, 40 and 50 g urea/kg DM of steam-treated oil palm fronds (OPF) and dry matter intake tended (p>0.05) to increase with increasing urea supplementation up to 30 g/kg OPF (77.7 g/kg $BW^{0.75}$), but decreased (p<0.05) with 40 and 50 g urea/kg OPF (67.4 and 63.7 g/kg BW0.75, respectively) supplementation. Similarly, dry matter, organic matter, crude protein, neutral detergent fiber and hemicellulose digestibilities increased (p<0.05) with the addition of urea to 30 g/kg OPF but thereafter decreased (p<0.05) with 40 and 50 g/kg OPF. Ruminal pH, ruminal $NH_3$-N concentration and plasma urea concentration increased linearly (p<0.01) and quadratically (p<0.01) as a consequence of addition of urea to the diet. Excretion of total purine derivatives (PD) by goats fed 30 g of urea/kg OPF was highest (p<0.05) followed by goats fed 20, 40, 10 and 50 g of urea/kg OPF. Microbial N (g N/day) and efficiency of microbial N supply expressed as g N/kg organic matter apparently digested in the rumen were higher (p<0.05) in goats fed 30 g of urea/kg OPF (5.5 g N/day and 22.0 g N/kg DOMR, respectively) than in goats on 10 and 50 g of urea/kg OPF treatments. However, the former did not differ from goats fed 20 g of urea/kg OPF (3.9 g N/day and 16.6 g N/kg DMOR, respectively). Ruminal VFA concentration, protein/energy ratio, N absorption and N retention increased (p<0.05) with the addition of urea to the diet up to 30 g/kg OPF but decreased (p<0.05) with 40 and 50 g/kg OPF. This implies that the optimal level of urea supplementation in an OPF based diet was about 30 g urea/kg OPF.

EFFECT OF ADDITION OF INTACT OR ALKALIZED LUCERNE JUICE AT ENSILING ON THE NUTRITIVE VALUE OF RICE STRAW SILAGE

  • Nishino, N.;Ohshima, M.;Yokota, H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.5 no.3
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    • pp.487-494
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    • 1992
  • This experiment was conducted to study the effect of addition of Lucerne juice (LJ) obtained by mechanical extraction of freshly harvested crop on the nutritive value of rice straw silage. Rice straw (RS) was ensiled with intact, NaOH or $NH_3$ treated LJ at 3:7 ratio on fresh weight basis (LJ RS, LJ NaOH RS and LJ $NH_3$ RS, respectively). Each alkali was mixed with fresh juice at a level of 4% of rice straw dry matter just before ensiling. Rice straw ensiled with water was prepared as the control (W RS). In the digestion trial, goats were allocated in a $4{\times}4$ Latin-square design and fed the diet containing three parts of RS silage and one part of wheat bran (DM basis). For the goats receiving the control silage, urea was supplemented at feeding time so as to adjust the nitrogen intake except for goats on LJ $NH_3$ RS silage. Crude protein content of RS silage was increased from 5.2 to 9.1% (DM basis) by the addition of intact LJ and to about 24% by $NH_3$ treated LJ. The control W RS silage contained only trace amount of lactic acid and was dominated by acetic and butyric acid. The addition of intact LJ reduced butyric acid content and $NH_3-N/TN$ of the silage whereas the addition of alkalized LJ increased those values and shifted to a butyrate type fermentation. Nutrient digestibilities and nitrogen balance of goats were almost the same when they were fed W RS and LJ RS silage indicating the addition of intact LJ did not improve the nutritive value. The addition of alkalized LJ significantly increased the fiber digestibilities of RS silage and $NH_3$ treatment was more effective than NaOH treatment. Postprandial ruminal $NH_3-N$ and blood urea nitrogen (BUN) concentrations were decreased by feeding LJ NaOH RS silage suggesting ruminal protein synthesis was enhanced along with the increase of energy supply for supply for rumen microbes by the alkali treatment. The advantageous fiber digestibilities of LJ $NH_3$ RS silage compared with those of LJ NaOH RS silage might be attributable to a sufficient nitrogen supply for microbial fiber digestion in the rumen.

Nitrogen Utilization of Cell Mass from Lysine Production in Goats

  • Seo, S.;Kim, H.J.;Lee, S.Y.;Ha, Jong K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.4
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    • pp.561-566
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    • 2008
  • Two experiments were conducted to evaluate nutritive value of cell mass from lysine production (CMLP) as a protein supplement for ruminants. In each experiment, animals were fed a diet containing 40% of forages and 60% of concentrates, mainly composed of rice straw and ground corn, respectively, to meet the maintenance requirements, and the diets were formulated to supply equal amounts of energy and nitrogen among treatments. In order to investigate the effect of CMLP on ruminal fermentation (Experiment 1), three Korean native goats weighing $26.1{\pm}1.4kg$ were allotted into individual cages with a $3{\times}3$ Latin square design. Each animal was fed one of three protein sources (CMLP, soybean meal (SBM), and urea). Rumen pH, bacterial and fungal counts, volatile fatty acid concentrations and acetate to propionate ratio were not significantly different among treatments. Concentration of propionate, however, was higher in SBM treatment (14.1 mM) than in CMLP (8.7 mM) or urea (9.3 mM) treatments. There was significantly more branch-chain volatile fatty acid production in CMLP (1.9 mM) and SBM (1.8 mM) treatments than in urea (1.3 mM) treatment. The number of protozoa was the highest in urea treatment, followed by CMLP and SBM treatment with significant differences. A metabolic trial (Experiment 2) was conducted to measure in vivo nutrient digestibility and nitrogen retention in Korean native goats fed CMLP and SBM. Two heavy ($35.0{\pm}1.2kg$) and two light ($25.0{\pm}0.9kg$) Korean native goats, caged individually, were used in this experiment. A heavy and a light animal were paired and supplemented with either CMLP or SBM. The animals fed CMLP showed a trend of lower total tract digestibility in all the nutrients measured; however, there was no statistical significance except for digestibility of ether extract. Nitrogen digestibility of CMLP was estimated to be about 7% units lower than that of SBM. There was a tendency for lower nitrogen retention in CMLP treatment (35.9%) compared to SBM treatment (42.3%). In summary, CMLP can be a good protein source for ruminant animals from nutritional and economic perspectives and may replace some, if not all, of SBM in a diet without losing nitrogen utilization efficiency. Further research is warranted for investigating the effect of CMLP fed with easily fermentable forage and the effective level of CMLP for replacing SBM.

Backfat Characteristics of Barrows and Gilts Fed on Tuna Oil Supplemented Diets during the Growing-finishing Periods

  • Jaturasitha, S.;Srikanchai, T.;Chakeredza, S.;ter Meulen, U.;Wicke, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.8
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    • pp.1214-1219
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    • 2008
  • This study was conducted to evaluate the effect of supplementing tuna oil to diets of growing-finishing pigs (barrows and gilts) on backfat characteristics when slaughtered at different weights. Four hundred and eighty crossbred (Large White$\times$Landrace$\times$Duroc) pigs averaging 30 kg were allotted to 12 treatment combinations (40 pigs/treatment combination) in a completely randomized design with a $2{\times}2{\times}3$ factorial arrangement of treatments. The treatments were: dietary tuna oil supplementation (0 and 2%); sex (barrows and gilts); and slaughter weight (90, 100 and 110 kg). As pigs reached their slaughter weight, they were randomly selected (8 pigs/treatment combination; 96 pigs in total) and slaughtered. Backfat colour, hardness and fatty acid profile were assessed. There were significant (p<0.05) differences in colour (L* and a* values) among treatments. Backfat of the control group was harder than on the tuna oil (p<0.001) and that of barrows was harder than of gilts (p<0.05). In addition, the thiobarbituric acid reactive substances (TBARS) values of fat from the tuna oil group stored for 3 days were higher (p<0.001) than the control group. The TBARS values of gilts tended to be higher than those of barrows and increased with increasing slaughter weight in the tuna oil group. The cholesterol and triglyceride levels were not affected by diet and sex but the triglyceride level increased with increasing slaughter weight (p<0.01). The tuna oil group had higher polyunsaturated fatty acid (PUFA) content, ratio of PUFA: saturated fatty acid (SFA) and total n-3 fatty acids but lower monounsaturated fatty acids content and n-6:n-3 fatty acids than the control group (p<0.01). Gilts had higher PUFA and n-6 fatty acids in backfat than barrows (p<0.05). The backfat from both 90 and 100 kg slaughter-weight groups had a lower ratio of n6:n3 fatty acid than the 110 kg slaughter-weight group (p<0.05). However, this was more pronounced in the tuna oil group. The PUFA: SFA was also increased while the n-6:n-3 ratio tended to reach the recommended levels for healthy eating in human beings of <5. However, due to oxidative susceptibility, barrows should not be slaughtered at more than 100 kg for the meat to be acceptable to consumers.

Choline supplementation improves the lipid metabolism of intrauterine-growth-restricted pigs

  • Li, Wei;Li, Bo;Lv, Jiaqi;Dong, Li;Zhang, Lili;Wang, Tian
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.5
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    • pp.686-695
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    • 2018
  • Objective: The objective of this study was to investigate the effects of dietary choline supplementation on hepatic lipid metabolism and gene expression in finishing pigs with intrauterine growth retardation (IUGR). Methods: Using a $2{\times}2$ factorial design, eight normal birth weight (NBW) and eight IUGR weaned pigs were fed either a basal diet (NBW pigs fed a basal diet, NC; IUGR pigs fed a basal diet, IC) or a diet supplemented with two times more choline than the basal diet (NBW pigs fed a high-choline diet, NH; IUGR pigs fed a high-choline diet, IH) until 200 d of age. Results: The results showed that the IUGR pigs had reduced body weight compared with the NBW pigs (p<0.05 from birth to d 120; p = 0.07 from d 120 to 200). Increased (p<0.05) free fatty acid (FFA) and triglyceride levels were observed in the IUGR pigs compared with the NBW pigs. Choline supplementation decreased (p<0.05) the levels of FFAs and triglycerides in the serum of the pigs. The activities of malate dehydrogenase and glucose 6-phosphate dehydrogenase were both increased (p<0.05) in the livers of the IUGR pigs. Choline supplementation decreased (p<0.05) malate dehydrogenase activity in the liver of the pigs. Gene expression of fatty acid synthase (FAS) was higher (p<0.05) in the IC group than in the other groups, and choline supplementation decreased (p<0.05) FAS and acetyl-CoA carboxylase ${\alpha}$ expression in the livers of the IUGR pigs. The expression of carnitine palmitoyl transferase 1A (CPT1A) was lower (p<0.05) in the IC group than in the other groups, and choline supplementation increased (p<0.05) the expression of CPT1A in the liver of the IUGR pigs and decreased (p<0.01) the expression of hormone-sensitive lipase in both types of pigs. The gene expression of phosphatidylethanolamine N-methyltransferase (PEMT) was higher (p<0.05) in the IC group than in the other groups, and choline supplementation significantly reduced (p<0.05) PEMT expression in the liver of the IUGR pigs. Conclusion: In conclusion, the lipid metabolism was abnormal in IUGR pigs, but the IUGR pigs consuming twice the normal level of choline had improved circulating lipid parameters, which could be related to the decreased activity of nicotinamide adenine dinucleotide phosphate-generating enzymes or the altered expressions of lipid metabolism-related genes.

Assessment of the Contribution of Risk Factors that Cause Cardiovascular Disease in Koreans (한국인의 심혈관질환 발생을 유발하는 위험인자들의 기여도 평가)

  • Cho, Sang-Ok
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.6
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    • pp.592-602
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    • 2020
  • This study examined the contribution of the risk factors that cause cardiovascular disease in Koreans. The subjects were 20,012 adults aged 30~74 years who were enrolled from the 2005~2013 Korean National Health and Nutrition Examination Survey, had filled the risk factors data, and had no cardiovascular disease history. Data analysis was conducted using SAS 9.3, and a weighted composite sample design analysis was performed. The contribution was obtained by applying a squared semi-partial correlation. As a result, they were higher in the order of age (63.9%), smoking (17.3%), systolic blood pressure (7.9%), diabetes (6.9%), antihypertensive medication use (3.0%), and BMI (0.9%) in men. In women, the contributions were in the order of age (54.1%), systolic blood pressure (23.5%), diabetes (13.6%), antihypertensive medication use (5.1%), smoking (3.1%), and BMI (0.6%%). According to age bracket analysis, smoking (51.8~33.7%) was the biggest risk factor, followed in order by age (26.6%) in their 30s and systolic blood pressure (17.3~21.0%) in those older than 40 years in men. In women, the systolic blood pressure (56.2~40.5%) was the main contributor to the risk factors, followed by age (24.0%) in their 30s and diabetes (13.6~32.6%) in those older than 40 years. The contribution of the income level was similar. Therefore, to prevent cardiovascular disease, various programs that reflect the contributions of the risk factors should be developed. Moreover, it is recommended to use the results shown in this study as basic data.

Development of Assessment Tools for Scientifically Gifted and Talented with Lower Grades in Elementary School (초등학교 저학년 학생을 위한 종합적 과학재능 검사 도구의 개발 -수행형 검사 수행을 위한 시사점 도출-)

  • Seo, YoonKyung;Jhun, Youngseok
    • Journal of The Korean Association For Science Education
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    • v.40 no.3
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    • pp.347-358
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    • 2020
  • He purpose of this study is to design and apply a pilot assessment tools for scientifically gifted and talented elementary school students with lower grades. The assessment tool consists of three parts: homeroom teachers' recommendation, paper and pencil test and performance tests. The tools are verified whether they are suitable for unique characteristics of young children and enable to attract active participation. For suitability check, students' performance tests were inductively analyzed and 30 behavioral patterns were shown which were included and partially exceeded the level of lower elementary students' performance expectation in NGSS. As a result, we concluded that assessment tool developed in this study will be effective in discriminating young pupils' scientific talents. Then for participation check, we compared the number of coding references as an indicator of participation. Two cases were found that students with high interest participated passively in performance tests. We found these 'passive participants' had excessive scientific experiences and extremely narrow region of interest, during the process of complex interpretation between the results of this assessment tool and in-depth interviews with homeroom teachers. We found out in this study that newly developed tools can be used in school scene after modifying and elaboration through accumulation of more case studies.

A study of the relationship between corporate governance and real earnings management: Based on foreign investors and growth (기업지배구조와 실제이익조정의 관계 연구: 외국인투자자와 성장성을 중심으로)

  • Kang, Shin-Ae;Kim, Tae-Joong
    • Journal of Distribution Science
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    • v.12 no.4
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    • pp.85-92
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    • 2014
  • Purpose - This study conducted empirical research on non-financial corporations listed on the stock exchange from 2001 to 2010, focusing on the effects of corporate governance on real earnings management of corporations. In particular, this study examined primarily the impact of the largest shareholder who could use earnings management to pursue his own self-interest, and foreign investors who played a checking role against the largest shareholders. The study also reviewed the relationship between corporate governance and earnings management while also considering corporate growth. Research design, data, and methodology - As for the measurements of real earnings management, abnormal operating cash flow and abnormal production cost were utilized. As for the independent variables, share ratio of the largest shareholder and affiliate person (M) and share ratio of foreign investors (FT) were leveraged. This study excluded those organizations that had changed their fiscal years, those that had not submitted an audit report, corporations under supervision, delisted corporations, corporations that had changed their business type, and so on, from the non-financial corporations out of the publicly traded corporations whose fiscal year ended in December from 2001 to 2010 in addition, KIS values were utilized for the corporate financial data in the study. To verify whether management structure and growth had an impact on real earnings management of a corporation through empirical analysis, a multiple regression analysis model was applied. Result - First, as a result of the analysis, the share ratio (M) of the largest shareholder and affiliate person was found to have a significant positive correlation with abnormal cash flow from operations(ACF) and abnormal production cost (APD). When controlling the growth, the share ratio (M) of the largest shareholder and affiliate person was found to have an insignificant correlation with abnormal cash flow from operations(ACF) but a significant correlation with abnormal production cost (APD). Second, foreign ownership (FT) was found to have a significant positive correlation with abnormal cash flow from operations(ACF) and abnormal production cost (APD) at the confidence level of 1 percent when not including the growth dummy. When controlling the growth, foreign ownership (FT) was found to have a significant negative correlation with abnormal cash flow from operations (ACF) and with abnormal production cost (APD). Conclusion - The results imply that the largest shareholder is closely related to earnings management through real activities regardless of corporate growth. It is also possible to determine from these results that foreign investors are related to earnings management through real activities when not considering corporate growth, but that they would reduce earnings management in the case of considering the growth. Thus, this study verified along with the existing studies that foreign investors were conducting the control function on controlling shareholders.

Effectiveness of consumer education through Home Economics on middle school students' consumer competency (가정교과의 소비자교육이 중학생의 소비자역량에 미치는 효과)

  • Park, Mi Jeong
    • Journal of Korean Home Economics Education Association
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    • v.30 no.2
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    • pp.1-20
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    • 2018
  • The purposes of this study were to investigate the intensity of middle school students' consumer competency and to assess effectiveness of Home Economics education on consumer competency. One group pretest-posttest quasi experimental design was employed to assess the effectiveness of Home Economics education on 1st grade middle school students' consumer competency in Gyeonggi province and 154 data copies were used for analyses. The results obtained were as follows. First, the cognitive consumer competency of the middle school 1st grade students was lower than the median of 37.66 out of 100 points, and the practical consumer competency was slightly higher than the median of 3.26 out of 5 points. As for cognitive competency, citizen competency was higher than deal competency. As for practical competency, deal competency was higher than citizen competency. Second, quasi-experiment intervention statistically enhanced the middle school students' consumer competency including the cognitive and practical competency. Third, the middle school students' perceived helpfulness of Home Economics education, perceived importance of deal competency and citizen competency, the level of cognitive citizen competency were statistically influential to practical consumer competency(deal and citizen competency). However, gender, life satisfaction, and cognitive citizen competency were not influential to practical consumer competency(deal and citizen competency). The outcome offered the basic information of middle school students' consumer competency and confirmed the effectiveness of Home Economics education on the middle school students' consumer competency.