• Korean Journal of Microbiology
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• 제32권2호
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• pp.102-108
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• 1994

Autonomously replicating sequence Binding Factor 1(ABF1) is a DNA-binding protein that specifically recognizes the $RTCRYN_5ACG$ at many sites in the yeast genome including the promoter element, mating-type silencer and ARS. To express the intact full-length ABF1 gene in E. coli, the ABF1 gene has been cloned into pMAL-c2 and His-61, Leu-353 and Leu-360 were substituted with other amino acid. ABF1 fusion proteins of wild type ABF1 and H61A, L353R and L360R nutants were purified by amylose resin affinity chromatography. Fusion protein of MBP and ABF1 was digested by Factor Xa and Characterized by gel retardation assay and complementation test. As aresult, we suggested that other DNA binding motif except atypical inc-finger motif is in the middle region of ABF1.

• Korean Journal of Plant Tissue Culture
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• 제21권5호
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• pp.253-275
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• 1994

Many flowering plants possess genetically controlled self -incompatibility (SI) system that prevents inbreeding and promotes outcrosses. SI is usually controlled by a single, multiallelic S-locus. In gametophytically controlled system, SI results when the S-allele of the pollen is matched by one of the two S-alleles in the style, while in the sporophytic system self-incompatible reaction occurs by the interaction between the pistil genotype and genotype of, not the pollen, but the pollen parent In the former system the self-incompatible phenotype of pollen is determined by the haploid genome of the pollen itself but in the latter the pollen phenotype is governed by the genotype of the pollen parent along with the occurrence of either to-dominant or dominant/recessive allelic interactions. In the sporophytic type the inhibition reaction occurs within minutes following pollen-stigma contact, the incompatible pollen grains usually failing to germinate, whereas in gametophytic system pollen tube inhibition takes place during growth in the transmitting tissue of the style. Recognition and rejection of self pollen are the result of interaction between the S-locus protein in the pistil and the pollen protein. In the gametophytic SI the S-associated glycoprotein which is similar to the fungal ribonuclease in structure and function are localized at the intercellular matrix in the transmitting tissue of the style, with the highest concentration in the collar of the stigma, while in the sporophytic SI deposit of abundant S-locus specific glycoprotein (SLSG).is detected in the cell wall of stigmatic papillae of the open flowers. In the gametophytic system S-gene is expressed mostly at the stigmatic collar the upper third of the style length and in the pollen after meiosis. On the other hand, in the sporophytic SI S-glycoprotein gene is expressed in the papillar cells of the stigma as well as in e sporophytic tape is cells of anther wall. Recognition and rejection of self pollen in the gametophytic type is the reaction between the ribonuclease in the transmitting tissue of the style and the protein in the cytoplasm of pollen tube, whereas in the sporophytic system the inhibition of selfed pollen is caused by the interaction between the Sycoprotein in the wall of stigmatic papillar cell and the tapetum-origin protein deposited on the outer wall of the pollen grain. The claim that the S-allele-associated proteins are involved in recognition and rejection of self pollen has been made merely based on indirect evidence. Recently it has been verified that inhibition of synthesis of S$_3$ protein in Petunia inflata plants of S$_2$S$_3$ genotype by the antisense S$_3$ gene resulted in failure of the transgenic plant to reject S$_3$ pollen and that expression of the transgenic encoding S$_3$ protein in the S$_1$S$_2$ genotype confers on the transgenic plant the ability to reject S$_3$ pollen. These finding Provide direct evidence that S-proteins control the s elf-incompatibility behavior of the pistil.

• Journal of Preventive Medicine and Public Health
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• 제26권2호
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• pp.293-309
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• 1993

With expanded and extended coverage of the national medical insurance and fast growing health care expenditures, appropriateness of health service utilization and quality of care are concerns of both health care providers and insurers as well as patients. An accurate patient classification system is a basic tool for effective health care policies and efficient health services management. A classification system applicable to Korean medical information-Korean Diagnosis Related Groups (K-DRGs)-was developed based on the U.S. Refined DRGs, and the performance of the developed system was assessed in this study. In the process of the development, first the Korean coding systems for diagnoses and procedures were converted to the systems used in the definition of the U.S. Refined DRGs using the mapping tables formulated by physician panels. Then physician panels reviewed the group definition, and identified medical practice patterns different in two countries. The definition was modified for the differences in K-DRGs. The process resulted in 1,199 groups in the system. Several groups in Refined DRGs could not be differentiated in K-DRGs due to insufficient medical information, and several groups could not be defined due to procedures which were not practiced in Korea. However, the classification structure of Refined DRGs was retained in K-DRGs. The developed system was evaluated fur its performance in explaining variations in resource use as measured by charges and length of stay(LOS), for both all and non-extreme discharges. The data base used in this evaluation included 373,322 discharges which was a random sample of discharges reviewed and payed by the medical insurance during the five-month period from September 1990. The proportion of variance in resource use which was reduced by classifying patients into K-DRGs-r-square-was comparable to the performance of the U.S. Refined DRGs: .39 for charges and .25 for LOS for all discharges, and .53 for charges and .31 for LOS for non-extreme discharges. Another measure analyzed to assess the performance was the coefficient of variation of charges within individual K-DRGs. A total of 966 K-DRGs (87.7%) showed a coefficient below 100%, and the highest coefficient among K-DRGs with more than 30 discharges was 159%.

• Horticultural Science & Technology
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• 제28권3호
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• pp.381-386
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• 2010

The berry growth and pericarp characteristics were characterized to confirm the effects of bagging periods on berry cracking during berry development in 'Kyoho' grape. The berry weight was the highest at 13.4 g in late period of bagging treated at 7 to 9 weeks after full bloom (WAFB) as compared with the lowest of 12.3 g in total period bagging. The berry cracking rate under critical turgor pressure in the non-bagging control was 53.3%, while those of bagging treatments were decreased in the order of 42.7%, 37.3%, 33.3%, and 18.7% in bagged during 3 to 9, 3 to 5, 5 to 7, and 7 to 9 WAFB, respectively. In the results of observation on histological characteristics of pericarp, berry lenticels of whole bagging treatments had smaller and normal shape compared with non-bagging control treatment. Especially on the pericarp of late period bagged during 7 to 9 WAFB, suberization around stomata and micro-cracking were not observed and structural strength of pericarp was increased with thicker sub-epidermal layer and cell wall. Therefore, the results indicate that bagging treatment for two weeks just before the veraison when the day length and daylight is relatively longer and stronger can effectively reduce berry cracking by strengthening structure of pericarp in 'Kyoho' grape.

• Korean Journal of Food Science and Technology
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• 제28권1호
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• pp.44-52
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• 1996

Sensory properties of cooked rice were affected by the varieties and growing environments of the rice. Moistness, cohesiveness, and adhesiveness of cooked rice were highly and positively correlated each other, whereas firmness was negatively correlated with these attributes. Sixty rice samples which differed in varieties and/or growing environments were divided into four groups based on their textural properties through principal component analysis and cluster analysis. Quality type I showed the highest values for moistness, cohesiveness, and adhesiveness, and the lowest values for firmness of cooked rice. On the other hand, quality type IV showed just the opposite values. There was no significant difference among rice starches in amylose content (P<0.05). A17 (type III) and A09 (type IV) had higher blue values for starch and amylopectin than the other samples (type I and II). On the amylogram, these samples showed lower values for breakdown and higher values for setback than the other samples. Average degree of polymerization, average chain length, and average number of chains for amylose and amylopectin were 597-878 and 2660-3140, 140-230 and 17-19, and 3.1-4.9 and 140-170, respectively.

• Fire Science and Engineering
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• 제31권5호
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• pp.63-69
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• 2017

This study describes the development of a long-distance hose for ultra-high pressure operation, which can be used in conjunction with an ultra-high pressure pump and can be effectively applied to the fire suppression of high-rise buildings and a long, large tunnels. Also, it has phosphorescent properties, which can help to secure the withdrawal route of the fire-fighters when they are threatened by the fire. We developed an ultra-high pressure hose aiming at a pressure of 3 MPa and a flow rate of 2000 lpm and developed an ultra-high pressure fire hose that can withstand this very high pressure by using a double jacket, triple polyurethane coating and warf (Wp) of 52. In order to ensure the performance of the developed ultra-high pressure hose, its structure, appearance, leakage at high pressure, length and elongation were inspected by a certified certification agency, who also subjected it to a peeling test, friction test, breaking pressure test and free fall test. Also, it was studied in addition to the luminescent high-pressure hose for fire-fighting. In the phosphorescence test, the luminance measurement value was more than the reference value of the luminance test after 40 minutes, which confirmed that its performance was satisfactory for fire-fighting products. In the future, if such an ultra-high pressure fire hose were commercialized and applied in the field, it could contribute to securing improved fire suppression and safer exit from fires, as compared to the fire hoses currently used in the suppression of fires in skyscraper buildings and long tunnels.

• Journal of the Korea Academia-Industrial cooperation Society
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• 제12권11호
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• pp.5214-5218
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• 2011

In this paper, bandwidth improvement of a series-fed two dipole array(STDA) antenna applicable for mobile communication base station antennas is studied. The proposed STDA antenna consists of two strip dipole antennas with different lengths which are connected directly trough a coplanar stripline(CPS). By adjusting the spacing between the two dipoles and the length of the second dipole, the bandwidth of the STDA can be enhanced. In addition, an integrated balun composed of a short-circuited microstrip line and a slot line is utilized to minimize the area required for a feeding part, and a broadband impedance matching is obtained by adjusting the feeding point. Based on the proposed antenna structure, an STDA antenna covering the frequency band ranging from 1.75 GHz to 2.7 GHz, which includes almost all the existing mobile communication frequency bands, with more than 5 dBi gain is designed and fabricated on an FR4 substrate with dielectric constant of 4.4 and thickness of 1.6mm, and experimentally tested. The fabricated antenna shows impedance bandwidth of 49%(1.7-2.8 GHz) for VSWR<2, a gain higher than 5.5 dBi, and a front-back ratio better than 12 dB.

• Korean Journal of Fisheries and Aquatic Sciences
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• 제31권1호
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• pp.8-16
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• 1998

Sexual maturation and reproductive cycle of the goldeye rockfish, Sebastes thompsoni were investigated under photomicroscopy. Samples were collected monthly in the coastal water of Samcheonpo ($34^{\circ}55'N$ ), Korea from November 1995 to October 1996, The ovary consists of several ovarian lamellae originated from ovarian outer membrane. Oogonia which are originated from the inner surface of the ovarian lamella protrude to the ovarian cavity in oocyte stage, and they ave suspended by the egg stalk. The testis is seminiferous tubule type in internal structure. Seminiferous tubule consists of many testicular cysts which contain numerous germ cells in same developmental stage. Biological minimum size of female and male were 19.5 cm and 21.5 cm in total length, respectively. Gonadosomatic index (GSI) of female was the highest (9.56) in March and the lowest (0.15) in August. GSI of male was the highest (0.25) in February and the lowest (0.04) in July. Reproductive cycle was classified into the following successive stages: in female, growing (October and November), maturation ( $December\~February$), gestation (March), parturition and recovery ($April\~June$) and resting ($July\~September$), and in male, growing ($September\~November$), maturation ( December and January), ripe and copulation ( February and March) and degeneration and resting ($April\~August$).

• Proceedings of the Korea Water Resources Association Conference
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• 한국수자원학회 2019년도 학술발표회
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• pp.164-175
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• 2019

The Tor Tong Daeng Irrigation Project with the irrigation area of 61,400 hectares is located in the Ping Basin of the Upper Central Plain of Thailand where farmers depended on both surface water and groundwater. In the drought year, water storage in the Bhumipol Dam is inadequate to allocate water for agriculture, and caused water deficit in many irrigation projects. Farmers need to find extra sources of water such as water from farm pond or groundwater as a supplement. The operation of Bhumipol Dam and irrigation demand estimation are vital for irrigation water allocation to help solve water shortage issue in the irrigation project. The study aims to determine the smart dam operation system to mitigate water shortage in this irrigation project via introduction of machine learning to improve dam operation and irrigation demand estimation via soil moisture estimation from satellite images. Via ANN technique application, the inflows to the dam are generated from the upstream rain gauge stations using past 10 years daily rainfall data. The input vectors for ANN model are identified base on regression and principal component analysis. The structure of ANN (length of training data, the type of activation functions, the number of hidden nodes and training methods) is determined from the statistics performance between measurements and ANN outputs. On the other hands, the irrigation demand will be estimated by using satellite images, LANDSAT. The Enhanced Vegetation Index (EVI) and Temperature Vegetation Dryness Index (TVDI) values are estimated from the plant growth stage and soil moisture. The values are calibrated and verified with the field plant growth stages and soil moisture data in the year 2017-2018. The irrigation demand in the irrigation project is then estimated from the plant growth stage and soil moisture in the area. With the estimated dam inflow and irrigation demand, the dam operation will manage the water release in the better manner compared with the past operational data. The results show how smart system concept was applied and improve dam operation by using inflow estimation from ANN technique combining with irrigation demand estimation from satellite images when compared with the past operation data which is an initial step to develop the smart dam operation system in Thailand.

• Korean Journal of Remote Sensing
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• 제35권5_2호
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• pp.831-840
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• 2019

Recently, the span length of long-span bridges is getting longer. As a result, it has been suggested that a new concept called 'super long-span bridge'. In case of super long span bridges, the structure is being complicated and the importance of structural stability is being emphasized. However, until recently, the most commonly used sensors (dual axis clinometer, anemometer, strain gauge, etc.) have got limit about the bridge monitoring. Consequently, we researched the application of a Global Navigation Satellite System (GNSS) to improve the limit of the existing sensors. In this study, the dual axis clinometer, the anemometer and the strain gauge together with the GNSS were used to analyze the behavior of a super-long suspension bridge. Also, we propose the detailed method of bridge monitoring using the GNSS. This study consisted of three steps. First step calculated the absolute coordinates of the towers and the longitudinal axis direction of the study bridge using the GNSS. In second step, through the analysis of the long-term behavior in shortly after construction, we calculated the permanent displacement and evaluated the stability of main towers. Third step analyzed the behavior of bridge by the wind direction and was numerically indicated. Consequently, the bridge measurement using the GNSS appeared that the acquired data is able to easy processing according to the analysis purpose. If we will use together the existing measurement sensors with the GNSS on the maintenance of the super long-span bridge, we figure each error of measurement data and improve the monitoring system through calibration. As a result, we acquire the accurate displacement of bridge and figure the behavior of bridge. Consequently, we identified that it is able to construct the effective monitoring system.