• Journal of Microbiology and Biotechnology
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• v.14 no.2
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• pp.415-416
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• 2004

Crystals of six new Bacillus thuringiensis serovarieties [coreanensis (H25), konkukian (H34), leesis (H33), seoulensis (H35), sooncheon (H41), and yosoo (HI8a18c)] with different H-antigens, which are toxic to Bombyx mori and/or mosquito larvae, were serologically quite distinct from each other.

• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.509-514
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• 2004

Six new serovarieties of B. thuringiensis carrying specific H-antigen have minor differences in biochemical characteristics and morphological characteristics of crystals, which are commonly resistant against four antibiotics. The B. thuringiensis serovar. coreanensis is nontoxic to silkworm larvae, but it is moderately toxic against the Culex pipiens larvae. The B. thuringiensis serovar. konkukian and leesis are nontoxic against mosquitos larvae, but are toxic against silkworm larvae. The B. thuringiensis serovar. seoulensis, sooncheon, and yosoo are highly toxic to B. mori larvae and moderately toxic to C. pipiens larvae. The six serovarieties harbor different plasmid DNA patterns. A 102-kDa protein is a major crystal protein in the four serovarieties and a 86-kDa protein is in one serovariety.

• Journal of Microbiology and Biotechnology
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• v.4 no.4
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• pp.290-295
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• 1994

30 monoclonal antibodies (mAbs) were produced against Bacillus thuringiensis subsp. canadensis. Out of the these, 6 mAbs were selected for further studies. SDS-PAGE analyses of sonicated antigens of 10 8. thuringiensis strains showed that they generally had both predominant protein antigens of molecular weights of 45 kilodalton (kd) except for shandogiensis and konkukian, and 37kd except for israelensis, tochigiensis, and shandogiensis, respectively. These results indicate that 4kd and 37kd may be important for demonstrating common antigens except for a few strains of B. thuringiensis. In comparing the result of the westem blot using mAbs with that of using polyclonal antibodies to canadensis, we found that immunoreactive proteins of 99 and 39 kd were identified as common antigens, which might act as antigenic determinants, and might be surface or flagella antigens. Reactivities of mAbs with 41 strains of 8. thuringiensis demonstrated that mAbs of C-1, C-3, C-4, C-S and C-6 except C-2 did not recognize epitopes of thuringiensis, but that all of the mAbs recognized epitopes of galleriae, kurstaki, dakota, tolrJokuensis, silo, toguchini, and leesis. The potential applications of the mAbs we produced would be useful tools for the clarification of taxonomy, investigation of antigenic relationship between B. thuringiensis strains, and localization of specific surface and flagella antigens.