• 한국응용곤충학회지
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• 제32권1호
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• pp.115-121
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• 1993

자나방과의 꼬마분홍애기자나방, Idaea nielseni (Hedemaon) ; 흰꼬마애기물결자나방, Asthena achrifasciaria Leech ; 어깨둥근가지나방, Pseudepion magnaria (Wileman) ; 갈고리가지나방, Heterocallia lemeraria (Swinhoe) ; 흰띠가지나방, Pseuderannis lomozemia(Proul) ; 줄점겨울자나방, Larerannis orthogrammaria (Wehrh) ; 흑백가지나방, Phigalia sinuosaria Leech ; 이른봄넓은띠가지나방, Phigaliohybernia fulvinfula lonue ;이른봄긴날개가지나방, Planociampa modesta(Butler)등 9종을 우리날 미기록종으로 보고한다.

• Applied Microscopy
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• 제27권3호
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• pp.309-320
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• 1997

The spermatogenesis of korean leech, Whitmania edentula was observed, using both light and electron microscopes. The spermatogonium and maturing spermatozoon are connected with long cytoplasmic process to the cytophore, which supplys nutrition to the germ cells and supports synchronous maturity. The truck of korean leech is divided into three regions; a long ladder-shaped acrosome and head, long middle piece and long tail. Long head region twists to the dextral helix, and nuclei are surrounded with microtubules (manchette).The nebenkern formed with long mitochondrion exists in the middle pieces, and a long tail of Whitmania edentula ($9\times2+1$ axoneme) differs from the $9\times2+2$ axoneme of Rhynchobdellae. The late cytophore is mostly formed with crystalloid matter and a number of lysosomes, and matured spermatozooms are engulfed into the late cytophore.

• 한국응용곤충학회지
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• 제58권1호
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• pp.63-68
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• 2019

한국산 제비나방과 쌍꼬리나방아과의 3미기록종인Europlema nubifasciaria (Leech), Dysaethria meridiana (Inoue), Phazaca theclata ($Guen{\acute{e}}e$)을 보고한다. Europlema leleji는 Epiplema nubifasciaria의 동종이명으로 판명되었고, 후자는 Europlema속으로 새롭게 편입된다. Europlema nubifasciaria의 암컷생식기를 처음으로 기재한다. 채집된 종의 성충과 생식기의 사진을 제공한다.

• 한국어병학회지
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• 제30권2호
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• pp.97-105
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• 2017

Parasitic leeches could directly (through causing poor growth, anemia and wound in the fish) and indirectly (by predisposition of the fish to secondary bacterial and fungal infections) affects their hosts. In the present study, fishes that were attacked by leeches in natural and experimental environment were studied. Pathologic samples were obtained from damages at the site of leech bite, as well as kidney and liver of the fish. Histopathological examination revealed numerous lesions at the site of leech bite including tissue demolition, detachment at the site of leech bite in the epidermis of epithelial tissue in the skin, destructed nucleus in epithelial cells of the skin plus necrosis in the damaged skin and weak inflammatory penetration to acute necrotic damages along with piercing dermis layer. Pathologic lesions in the kidney included some changes such as proliferation by increasing glomerular cells and membrane cells in capillary vein of the kidney, blood cell necrosis in kidney with infiltration of white blood cells mainly mononuclear and less polymorphonuclear which are the symptoms of anemia due to blood feeding and sucking by leeches. There was also a chronic kidney infection probably originated from another part of body such as skin. Moreover, leeches caused hemorrhagic anemia due to blood consumption of the hosts, which led to observation of immature red blood cells. Also results showed that diseases induced by leeched in fish could be acute or chronic, which depends on size of fish, species of leech and severity of infection.

• BMB Reports
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• 제29권3호
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• pp.248-252
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• 1996

The epidermal organ of the leech contains a complex glycoprotein molecule of mucus. The mucus excreted from annelids plays Significant role in protection against desiccation and parasites. Mucus from the Korean native leech, H. nipponia, was investigated for biochemical characteristics for possible development of biomaterials of cosmetic and pharmaceutical agents. The leech skin mucus was heavily glycosylated mucin-like protein with a high molecular weight comprised 80% carbohydrate and 20% protein. Threonine, serine, and glycine were the major components of the isolated protein and these accounted for 50% of total amino acids. The carbohydrate portion contained glucosamine, galactosamine. galactose, glucose. mannose and sialic acid in oligosaccharide form linked with threonine and serine residues of the glycoprotein.

• 대한한방내과학회지
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• 제22권4호
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• pp.601-611
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• 2001

Objectives : We aimed to identify the dose-dependent inhibitory effects of Cheonsagunja-tang(喘四君子湯), leech(Hirudo medicinalis/水蛭) roasted with Ephedrae Herba(麻黃) on the mRNA expression of IL-6, IL-16, GM-CSF involved in the asthma model. Methods : In the study BEAS-2B cell lines, human epithelial cells were used. These cells were stimulated with tumor necrosis factor(TNF)-${\alpha}$ for artificial inflammatory expression. ${\beta}$-actin messenger RNA(mRNA) was used by internal standard. After 24 hours of the Cheonsagunja-tang, leech-treatment, total cellular RNAs were collected treating RNA zol directly on the living cells. Then the transcriptional activities of IL-6, 16 and GM-CSF were measured by RT-PCR with electrophoresis. Results: In the Cheonsagunja-tang study, the mRNA expression of IL-6 showed 30% transcriptional inhibitory effect compared to the control group in the $100{\mu}l/ml$ category(p<0.005). In the IL-16, there was 26%, 31% and 31% transcriptional inhibitory effect compared to the control groups in the $4{\mu}l/ml$, $20{\mu}l/ml$ and $100{\mu}l/ml$ categories, respectively(p<0.05). In the GM-CSF, the experimental group had 56% transcriptional inhibitory effect compared to the control group in the $100{\mu}l/ml$ category(p<0.001). In other concentrations, there was no inhibitory effect. In the leech study, the mRNA expression of IL-6 showed 37% transcriptional inhibitory effect compared to the control group in the $100{\mu}l/ml$ category(p<0.001). In the IL-16, there was 63% and 67% transcriptional inhibitory effect compared to the control groups in the $20{\mu}/ml$ and $100{\mu}/ml$ categories, respectively(p<0.001). In the GM-CSF, there was 64% and 68% transcriptional inhibitory effect compared to the control groups in the $20{\mu}l/ml$ and $100{\mu}l/ml$ categories, respectively(p<0.001). In other concentrations, there was no inhibitory effect. Conclusions : This study shows that Cheonsagunja-tang and leech roasted with Ephedrae Herba have dose-dependent inhibitory effects on the mRNA expression of IL-6, IL-16 and GM-CSF in BEAS-2B cell lines, human epithelial cells.

• 한국동물학회지
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• 제7권2호
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• pp.53-57
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• 1964

In this paper the author has newly recorded fifteen species of moths from Korea as follows : Acrodontis fumosa PROUT (Geometridae) , Dendrolimus yamadai NAGANO(Lasiocampidae), Mirina christophi STAUDINGER(Endromidae), Ramesa tosta WALKER (Notodontidae). Apamea scolopacina subbrunnea WAREEN(Noctuidae), Anuga multiplicans WALKER (Noctuidae), Bena karaeffti GRAESER(Noctuidae), Macrochochthonia fervens BUTLER (Noctuidae) ,Hyposada brunnea LEECH (Noctuidae), Catocala eminens STAUDINGER (Noctuidae), Bleptina contigua LEECH (Noctuidae), Dichromia orosia CRAMER (Noctuidae), Diarsia pacifica BOURSIN (Noctuidae), Nyctemera plagifera WALKER (arctiidae), Phyllosphingia dissimilis BREMER (Sphingidae).

• Parasites, Hosts and Diseases
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• 제52권2호
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• pp.169-175
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• 2014

There are 60 species of blood-feeding land leeches, 50 species belonging to the family Haemadipsidae and 10 species belonging to the family Xerobdellidae. Despite recent papers on the land leeches, their taxonomic identification is not fully understood, especially at a species level. In Korea, there have been no historical records of the terrestrial leeches, but recently an unrecorded blood-feeding land leech was discovered at Gageo-do (Island), Korea. Molecular analysis was used to identify the species of 29 leeches collected from Mt. Dock-Sil in Gageo-do. Conventional PCR was conducted using nuclear 18S rRNA and mitochondrial cytochrome c oxidase subunit 1 (CO1) genetic marker. The 18S rRNA sequences revealed that the leeches share 99.9% identity with Haemadipsa rjukjuana (inhabiting Taiwan), and the CO1 sequences revealed that the leeches are very close to H. rjukjuana (inhabiting Taiwan). The CO1 sequences were separated into 2 categories, 1 with 94.6% and the other with 94.3% similarity to the H. rjukjuana L00115A (inhabiting Taiwan). This new finding of the land leech is the first record in Korea. In addition, the north range of the distribution of the blood-feeding leech (Hirudiniformes: Haemadipisidae) should be reconsidered including Korea.

• Archives of Plastic Surgery
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• 제32권1호
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• pp.124-130
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• 2005

In the microsurgical era, replantation with microvascular anastomosis is considered as the most superior method in aspects of texture, color, shape in case of nose amputation. There are some reported cases of replantation in nose amputation historically, but most of them are composite graft cases rather than microvascular anastomosis. Only a few cases of successful nasal replantation with microvascular anastomosis have been reported due to the reason that the size of vessels is usually very small and identifying suitable vessels for anastomosis is difficult. Microanastomosis of artery and microanastomosis of vein are ideal in replantation, but identifying suitable veins is often difficult. Without venous anastomosis, resolving the venous congestion remains to be a problem. We can carry out arteriovenous shunt if we can find two arteries in amputee. However, the smaller the size of amputee is, the more difficult it is to find two arteries. Instead of arteriovenous shunt, we can try external venous drainage(frequently swab, pin-prick, stab incision, IV or local heparin injection, dropping, apply of heparin-soaked gauze, use of medical leech). Here, we present three cases of replantation with microscopical arterial anastomosis (one angular artery, two dorsal nasal arteries) and external venous drainage (stab incision, application of medical leech and heparin-soaked gauze) even though the size of amputee may be as small as $1.5{\times}1.0cm$. In all cases, surgical outcomes were excellent in cosmetic and functional aspects. This report describes successful replantation by microvasular anastomosis in case that suitable veins are not found.

• 한국응용곤충학회지
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• 제45권2호
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• pp.131-137
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• 2006

중국 장백산(백두산의 중국측 면) 나비목 조사에서(1999-2004) 밤나방과의 Herminiinae아과에 속한 27종이 확인되었다. 이 중 Paracolax fascialis (Leech), P. fentoni (Butler), Polypogon gryphalis Herrich-Sch$\"{a}$ffer, Sinarella japonica (Butler), S. punctalis (Herz), S. cristulalis (Staudinger) 등 6종은 중국에서 처음 보고되는 종이었고, Hadennia incongruous (Butler), Paracolax tristalis (Fabricius), Paracolax trilinealis (Bremer), Idia quadra (Graeser), Hydrillodes morosa (Butler), Herminia grisealis (Denis & Schifferm$\"{u}$ller), Sinarella nigrisigna (Leech) 등 7종은 장백산 지역에서 처음 발견된 종이었다. 이들 6종의 중국 미기록 종에 대한 분류학적 특징과 성충 및 암수 생식기의 사진을 제시한다.