• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.145.2-146
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• 2003

To investigate occurrence and variability of satsuma mandarin ( Citrus unshiu)-infecting viruses in Jeju island, several sets of diagnostic RT-PCR primers were designed and applied to samples collected randomly. Each primers set used in this survey was designed to detect Satsuma dwarf virus (SDV, Sadwavirus) and Citrus mosaic virus (CiMV) which is reclassified as an isolate of SDV (SDV-CiMV, Saduavirus). RT-PCR methods could detect SDV-CiMV and CTV from leaf . samples of unshui citrus. CTV was the prevalent and SDV-CiMV was not common in Jeju island. RT-PCR product of SDV-CiMV-JJl2 were cloned and sequenced. Sequence of the isolate revealed that it was 96.9 ％ identical to SDV-CiMV-Jp isolate at the nucleotide level. SDV-CiMV-JJl2 was propagated on Physalis floridana and sequencing of entire sequences of genome is in progress. Variability of SDV in Jeju island was confirmed by sequence comparisons and restriction mapping analysis.

• The Plant Pathology Journal
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• v.35 no.3
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• pp.243-256
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• 2019

Field surveys for Plum pox virus (PPV) infection were conducted in stone fruit orchards all over Bulgaria. In total, 1168 out of 3020 leaf samples from cultivated Prunus spp. and wildly growing P. cerasifera trees reacted positive for PPV in DASI-ELISA with the universal monoclonal antibody (MAb) 5B. Further ELISA analyses showed that 987 and 127 isolates belonged to PPV-M and PPV-D serotypes, respectively. The plum and P. cerasifera showed 82.0% and 50.5% levels of infection, respectively followed by the peach (40.0%) and the apricot (32.0%). Five hundred fifty one PPV isolates were further typed by IC-RT-PCR with PPV-Rec, -M and -D-specific primers, targeting (Cter)NIb-(Nter) CP genome region, as 125 isolates were sequenced. The results revealed the presence of PPV-Rec, PPV-M and PPV-D and mixed infections of these strains. PPV-Rec was the most prevalent strain (49.0%), followed by PPV-M (40.1%), while PPV-D was the less spread strain (8.2%). PPV-Rec was the most common strain in plums, including the eight "old-aged" trees from the region of the first Sharka discovery. PPV-M was the most prevalent strain in peach and apricot. Phylogenetic analyses on (Cter)NIb-(Nter)CP of the isolates were performed. PPV-Rec isolates formed a homogeneous group, while PPV-M isolates split into PPV-Ma and PPV-Mb subgroups. Five separated clades were formed by the analyzed PPV-D isolates. Nucleotide sequences of the partial CP coding region of the analyzed isolates revealed a slightly higher intra-strain genetic variability in PPV-Rec and PPV-M isolates, while that of PPV-D strain isolates was higher from the reported for these strains.

• Korean Journal of Remote Sensing
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• v.34 no.6_1
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• pp.1055-1066
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• 2018

Vegetation indices on the basis of optical characteristics of vegetation can represent various conditions such as canopy biomass and physiological activity. Those have been mostly developed with the large-scaled applications of multi-band optical sensors on-board satellites. However, the sensitivity of vegetation indices for detecting vegetation features will be different depending on the spatial scales. Therefore, in this study, the investigation of photochemical reflectance index (PRI), known as one of useful vegetation indices for detecting photosynthetic ability and vegetation stress, under the three spatial scales was conducted using multi-spectral camera installed in unmanned aerial vehicle (UAV),field spectrometer, and leaf reflectometer. In the leaf scale, diurnal PRI had minimum values at different local-time according to the compass direction of leaf face. It meant that each leaf in some moment had the different degree of light use efficiency (LUE). In early growth stage of crop, $PRI_{leaf}$ was higher than $PRI_{stands}$ and $PRI_{canopy}$ because the leaf scale is completely not governed by the vegetation cover fraction.In the stands and canopy scales, PRI showed a large spatial variability unlike normalized difference vegetation index (NDVI). However, the bias for the relationship between $PRI_{stands}$ and $PRI_{canopy}$ is lower than that in $NDVI_{stands}$ and $NDVI_{canopy}$. Our results will help to understand and utilize PRIs observed at different spatial scales.

• The Plant Pathology Journal
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• v.37 no.4
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• pp.339-346
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• 2021

Prevalence of tan spot of wheat caused by the fungus Pyrenophora tritici-repentis has become more prevalent in Oklahoma as no-till cultivation in wheat has increased. Hence, developing wheat varieties resistant to tan spot has been emphasized, and selecting pathogen isolates to screen for resistance to this disease is critical. Twelve isolates of P. tritici-repentis were used to inoculate 11 wheat cultivars in a greenhouse study in splitplot experiments. Virulence of isolates and cultivar resistance were measured in percent leaf area infection for all possible isolate x cultivar interactions. Isolates differed significantly (P < 0.01) in virulence on wheat cultivars, and cultivars differed significantly in disease reaction to isolates. Increased virulence of isolates detected increased variability in cultivar response (percent leaf area infection) (r = 0.56, P < 0.05) while increased susceptibility in cultivars detected increased variance in virulence of the isolates (r = 0.76, P < 0.01). A significant isolate × cultivar interaction indicated specificity between isolates and cultivars, however, cluster analysis indicated low to moderate physiological specialization. Similarity in wheat cultivars in response to pathogen isolates also was determined by cluster analysis. The use of diverse isolates of the fungus would facilitate evaluation of resistance in wheat cultivars to tan spot.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2017.04a
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• pp.45-45
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• 2017

On-site monitoring of vegetable growth parameters, such as leaf length, leaf area, and fresh weight, in an agricultural field can provide useful information for farmers to establish farm management strategies suitable for optimum production of vegetables. Unmanned Aerial Vehicles (UAVs) are currently gaining a growing interest for agricultural applications. This study reports on validation testing of previously developed vegetable growth estimation models based on UAV-based RGB images for white radish and Chinese cabbage. Specific objective was to investigate the potential of the UAV-based RGB camera system for effectively quantifying temporal and spatial variability in the growth status of white radish and Chinese cabbage in a field. RGB images were acquired based on an automated flight mission with a multi-rotor UAV equipped with a low-cost RGB camera while automatically tracking on a predefined path. The acquired images were initially geo-located based on the log data of flight information saved into the UAV, and then mosaicked using a commerical image processing software. Otsu threshold-based crop coverage and DSM-based crop height were used as two predictor variables of the previously developed multiple linear regression models to estimate growth parameters of vegetables. The predictive capabilities of the UAV sensing system for estimating the growth parameters of the two vegetables were evaluated quantitatively by comparing to ground truth data. There were highly linear relationships between the actual and estimated leaf lengths, widths, and fresh weights, showing coefficients of determination up to 0.7. However, there were differences in slope between the ground truth and estimated values lower than 0.5, thereby requiring the use of a site-specific normalization method.

• Korean Journal of Environmental Agriculture
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• v.40 no.3
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• pp.186-193
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• 2021

BACKGROUND: Before generating transgenic plant using the CRISPR-Cas9 system, the efficiency test of sgRNAs is recommended to reduce the time and effort for plant transformation and regeneration process. The efficiency of the sgRNA can be measured through the transient expression of sgRNA and Cas9 gene in tomato cotyledon; however, we found that the calculated efficiency showed a large variation. It is necessary to increase the precision of the experiment to obtain reliable sgRNA efficiency data from transient expression. METHODS AND RESULTS: The cotyledon of 11^th, 15^th, 19^th, and 23^rd-day-old tomato (Solanum lycopersicum cv. Micro-Tom) were used for expressing CRISPR-Cas9 transiently. The agrobacterium harboring sgRNA for targeting ALS2 gene of tomato was injected through the stomata of leaf adaxial side and the genomic DNA was extracted in 5 days after injection. The target gene edition was identified by amplifying DNA fragment of target region and analyzing with Illumina sequencing method. The target gene editing efficiency was calculated by counting base deletion and insertion events from total target sequence read. CONCLUSION: The CRISPR-Cas9 editing efficiency varied with tomato cotyledon age. The highest efficiency was observed at the 19-day-old cotyledons. Both the median and mean were the highest at this stage and the sample variability was also minimized. We found that the transgene of CRISPR-Cas9 system was strongly correlated with plant leaf development and suggested the optimum cotyledon leaf age for Agrobacterium-mediated transfection in tomato.

• Proceedings of the Korea Water Resources Association Conference
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• 2005.05b
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• pp.908-911
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• 2005

지표와 대기사이에서 식물의 광합성 및 증발산 능력과 밀접하게 관련되어 있는 엽면적지수는 식생밀도를 나타낼때 사용되는 식생지수이다. 본 연구에서는 NOAA-AVHRR 자료를 사용한 엽면적지수를 사용하여 남한지역 엽면적지수의 시공간변화도와 주기성분석을 실시하였고 소양강댐유역, 안동댐유역과 주요 도시의 엽면적지수의 월별, 년별, 계절별 추세를 분석하였다. 추세분석결과 월추세 분석에서는 장기간의 뚜렷한 변동성을 나타내지는 않았지만 겨울과 봄 보다는 여름과 가을에 식생의 밀도가 더 높은 계절적인 요인에 많은 영향을 받는 것을 볼 수 있었다. 시간변화 분석결과 계절변화를 제외한 특별한 장기변동을 확인할 수 없었으며 엽면적지수의 공간 변화는 정규식생지수가 보여주는 지형이나 위도의 변화와 밀접한 관계를 가지는 변화양상을 보여주지 않았다. 이는 농지 및 삼림 등의 인위적 조성으로 인한 엽면적지수의 공간변화도 양상과 관계있다 하겠다.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.145.1-145
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• 2003

This study was conducted to designing conserved regions of molecules for virus-derived resistance to transgenic Phlaenopsis orchids to protect against two major orchid viruses, Cymbidum mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV). Infected leaf samples of Phalaenopsis were randomly screened by the RT-PCR with specific primers to both of viruses. RT-PCR products of the viruses were cloned and their nucleotide sequences were determined. Multiple alignments of coat protein (CP) genes of the viruses revealed that over the 88 ％ and 94 ％ identities with CymMV and ORSV, respectively, were observed. These data can be useful for selection of highly conserved regions of CP gene of the viruses for transgenic orchid experiments.

• International Journal of Industrial Entomology and Biomaterials
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• v.48 no.2
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• pp.78-85
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• 2024

Bud sport (bud mutation) is a valuable source for existing new genotypes in mulberry (Morus spp.) as well as critical materials for studying the molecular mechanisms underlying essential traits. Thus, identification, collection, characterization, and conservation of such natural variants are prerequisites for enhancing the mulberry genetic resource in the germplasm. In this context, we identified and characterized three bud sports (VBS-1, VBS-2, and VBS-3) of a popular mulberry cultivar, Victory-1 (V-1). These bud sports are morphologically, anatomically, and genetically more distinct from their mother plant, Victory-1. Moreover, these bud sports display lower growth and yield potential. Furthermore, these showed remarkably lower 2C DNA contents of 0.74 pg (VBS-1), 0.78 pg (VBS-2), and 0.76 pg (VBS-3), when compared to their mother plant V-1 (2C = 0.81 pg). On the other hand, molecular characterization between the bud sports and their mother plant revealed the existence of genetic variation due to the natural bud mutation that occurred in the mulberry cultivar Victory-1.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.459-464
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• 2001

Leaf disks from cultivar 'Kennebec' and one selection line (ND 860-2) were cultured on Murashige-Skoog medium with various combinations of indole acetic acid (IAA) and zeatin riboside. Shoots, roots and callus were induced at various combinations of plant growth regulator levels. The medium containing $3.5mg{\cdot}L^{-1}$ IAA and $4.0mg{\cdot}L^{-1}$ zeatin riboside produced the most plantlets. Rooted regenerants were grown in the greenhouse. The growth of regenerated plants obtained from the MS medium supplemented with $7.0mg{\cdot}L^{-1}$ IAA and $3.0mg{\cdot}L^{-1}$ zeatin riboside was significantly greater than those grown from nodal expalnts. In ND 860-2, a leaf chimera with chlorophyll deficient (light yellow) sectors was found in plants regenerated fiom leaf disks (grown on MS medium supplemented with $3.5mg{\cdot}L^{-1}$ IAA and $3.0mg{\cdot}L^{-1}$ zeatin riboside) but not in plants grown from nodal explants. The phenotypic variability was also observed in the tuber number, size and weight.