Kim, Ju;Lee, Sang-Koo;Kim, Jeong-Man;Kim, Tae-Heung;Moon, Hyung-Cheol;Choi, Kyu-Hwan;Choi, Dong-Chil
Korean journal of applied entomology
/
v.47
no.3
/
pp.237-248
/
2008
Development of T. urticae was studied on the leaves of eggplant grown in hydroponics with nitrogen contents of 5 mM, 10 mM, 30 mM, and 60 mM. As the nitrogen level in hydroponics increased, it also increased in the plant whereas that of K, Ca, and Mg decreased. More nitrogen in hydroponics resulted in increased contents of water and crude protein, and decreased ash, carbohydrates, and fibers within the plant. Biomass was the heaviest as 989.5 g at 10 mM and the lightest at 60 mM. Leaf thickness and the content of chlorophyll increased as the content of nitrogen increased. Laboratory leaf disc tests obtained from plants grown at various nitrogen levels revealed that feeding and oviposition preferences of T. urticae were high at 30 mM and low at 5 mM. Ratio of damaged leaf by naturally occurring T. urticae on eggplants of 99 days post-transplant in the greenhouse was the highest as 98% at 60 mM. Degrees of damage on eggplants with and without T. urticae infestation turned up more as the differences in the levels of nitrogen in the hydroponics get bigger. No definite differences in the rate of T. urticae development was found between nitrogen treatment levels but, mortalities in immature stages dropped as the nitrogen levels went up. Adult longevity was the longest of 11.9 for female and 6.9 days for male at 60 mM. Oviposition period was also the longest as 11.7 days at 60 mM and shortened as the level of nitrogen decreased. The number of eggs oviposited was the most as 144.4 at 60 mM while it was the least as 41.0 at 5 mM. Sex ratio was 0.75 in favor of female at 10 mM. $R_o$ and T increased, no trends were detected in $r_m\;and\;{\lambda}$, while Dt decreased as the levels of nitrogen went up.
This study was carried out to investigate the effect of selenium treatment on the growth of vegetables sprout. Four vegetables, such as cabbage, lettuce, pak-choi and leaf mustard were examined under various selenium treatments (0, 1, 5, 10, 25, 50, 100 mg $L^{-1}$). Seed germinations in cabbage, pak-choi and leaf mustard were significantly inhibited at high concentration of selenium treatment. However, seed germination in lettuce was not much inhibited. Growth characteristics, such as soot length, root length, fresh weight and chlorophyll contents, were not much decreased at 1 mg $L^{-1}$ of selenium and then significantly inhibited with the increase of selenium concentration at above 5 mg $L^{-1}$ in all four vegetables. The selenium content increased linearly with the increase of selenium concentration. At the range of 1 to 25 mg $L^{-1}$ of selenium treatment, selenium contents in vegetables were 0.11 to 1.15 of cabbage, 0.16 to 0.61 of lettuce, 0.13 to 1.31 of pak-choi and 0.14 to 1.13 mg $g^{-1}$dw of leaf mustard, respectively. These results showed that treatment of selenium with the range of 1 to 5 mg $L^{-1}$ could be used to produce the selenium enriched vegetable sprouts.
Pretreatment of chinese cabbage leaves with $100{\mu}M$ sodium nitroprusside (SNP), a nitric oxide (NO) donor, effectively improved their tolerance to subsequent $2{\mu}M$ paraquat (PQ)-induced oxidative damage. The fresh weight, and chlorophyll and protein contents in primary leaves treated with PQ alone were noticeably reduced over 24 h light incubation. However, these leaf injury symptoms were significantly alleviated with $100{\mu}M$ SNP pretreatment for 3 h prior to PQ exposure. In additions, the increase of the contents of malondialdehyde (MDA) and $H_2O_2$ due to PQ exposure were significantly inhibited by SNP pretreatment. Together with the protective effects of SNP against PQ toxicity in leaves, the changes of ascorbate-glutathione cycle enzymes activities were examined. In the PQ alone treatment, the activities of APX, DHAR, and GR after 6 h incubation were rapidly reduced and showed 19%, 50% and 39% respectively, compared with those of the control. However, the decreases in these enzyme activities were significantly inhibited by SNP pretreatment. As a result, their activities were higher than those of PQ alone treatment by 5 times, 2 times, and 1.5 times, respectively, at 6 h incubation. Thereafter, these enzymes decrease their activities gradually showing high levels than those of PQ alone. Based on the above results, it can be assumed that the activation of ascorbate-glutathione cycle by SNP pretreatment in chinese cabbage leaves exposed to PQ can prevent $H_2O_2$ accumulation, thereby leading to protection against PQ-induced oxidative stress. Also, these results indicate that NO acts as an protectant against PQ stress in the leaves of chinese cabbage.
Several physiological responses were investigated in plants treated with TOPE as a preliminary step to know its action site. Unlike photo-dependent diphenylethers, herbicidal activity of TOPE appeared slowly and its typical symptoms were both burning of leaf blades and abnormal division of meristem in grasses, Similarly, both leakage of cell electrolytes and the curling of cotyledon margin were also shown in cucumber(Cucumis sativus L.). Biosynthesis of chlorophyll in etiolated cucumber cotyledon was not inhibited directly by treatment of TOPE at low light intensity(5.5${\mu}$ mol $m^{-2}s^{-1}$ PAR) and protoporphyrin IX was not also accumulated. The contents of phytoene, phytofluene and ${\beta}$-carotene were abnormaly increased. Photosynthesis was inhibited only at high concentration. Mitochondrial respiration was inhibited at high concentration but rather increased significantly at 10${\mu}$M of TOPE. However, respiration inhibitors did not alleviate the two symptoms of TOPE in cucumber cotyledon. In the same experiments, using inhibitors of protein or nucleic acid biosynthesis, only one of the two symptoms was alleviated by chloramphenicol and cycloheximide. In contrast, both symptoms were alleviated by actinomycin-D and hydroxyurea, suggesting that nucleic acid metabolism might be preferentially related to the mode of action of TOPE. DNA, RNA and protein contents were accumulated in both cucumber cotyledon and rice (Oryza sativa L.) routs treated with TOPE, and the DNA of them was increased at first. Thus, it is conjectured that TOPE increase nucleic acid metabolism directly or indirectly, and then disturb various metabolic pathways causing abnormal physiological and morphological effects followed by final death.
Kim, Ho-Yong;Gwak, Ki-Seob;Kim, Hye-Yun;Ryu, Keun-Ok;Kim, Pan-Gi;Cho, Do-Hyun;Choi, Jin-Yong;Choi, In-Gyu
Journal of the Korean Wood Science and Technology
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v.39
no.6
/
pp.459-468
/
2011
The main purpose of this study was to examine the influence of treatment amounts of Slurry Composting and Biofiltration liquid fertilizer (SCBLF) on biomass growth of Yellow poplar (Liriodendron tulipifera) and to compare bioethanol production from the harvested wood. Relative growth rate, biomass production and leaf characteristics were significantly enhanced by SCBLF treatment and medium treatment plot showed highest value. Nitrogen compounds and water content in SCBLF affected to increase chlorophyll contents which led improving biomass production (64.67%) and glucose contents (6.07%) than control. Organosolv and dilute acid pretreatments were preliminarily carried for bioethanol production, and the pretreatment processes were conducted at all the same solid to liquid ratio (1 : 10), reaction temperature ($150^{\circ}C$), preheating time (40 min) and residence time (10 min). The water insoluble solid recovery of Organosolv pretreatment with 1% sulfuric acid as a catalyst was the lowest and that of medium treatment plot was 44.81%. Exchangeable cations in SCBLF might be affected to increase pretreatment effect. The simultaneous saccharification and fermentation process was followed to determine the ethanol production of the pretreated biomass. The highest ethanol production yield based on initial weight was obtained from high treatment plotby Organosolv pretreatment with 1% sulfuric acid (16.11%). But regarding biomass production, medium treatment plot produced most, and bioethanol production was increased by 72.93% than control.
A field research was conducted to evaluate the effects of inoculation of Rhizobium meliloti and lime application on the early growth of alfalfa, var. Luna, in an acid soil. Alfalfa seeds were inoculated by lime-peat pelleting method with $A_3$ strain isolated from alfalfa roots. Both inoculated and noninoculated seeds were sown in acid soil (pH 5.5) with three levels of lime application (1, 3, 6 ton/ha) on mid-April. Inoculated seeds produced many effective nodules in early stage and increased nitrogen and chlorophyll contents of leaf, consequently stimulating alfalfa growth. Pseudo nodules were found in noninoculated, while they were seldom formed in inoculated. Lime application increased soil pH, plant height, root length, and shoot and root weight. Liming effect on nodulation was significant at first cut, but no longer after then. Both inoculation and liming increased the forage yield of alfalfa. Inoculation and liming of 6 ton/ha together produced the highest yield. As compared to control plot of nitrogen application, the effect of seed inoculation only could be equal to about 120 kg/ha nitrogen application. The total nitrogen contents of forage were little affected by number or weight of nodules and amounts of lime application.
The present study aimed to investigate the effect of static magnetic field (SMF) on the growth and physiological characteristics of common indoor plant species. Five foliage plant species, Spathiphyllum spp., Ardisia pusilla DC., Syngonium podophyllum, Peperomia pereskiifolia, and Pilea cadierei were potted into plastic pot equipped with round type anisotropic sintered NdFeB permanent magnet inside the pot. The surface magnetic flux density of each magnet was 3,500 G. After 6 months of growth period, the biomass accumulations of Spathiphyllum, A. pusilla, and P. cadierei under SMF were statistically higher than those of controls. Tissue water content also increased under the influence of SMF in most species. The photosynthetic rate of Spathiphyllum under SMF significantly increased but other species showed no significant difference compared with control. Although there was no significant increase in the photosynthetic rates of A. pusilla, and P. cadierei, they showed remarkable increase in total fresh weight under SMF. This suggests that the demand of assimilates for normal metabolism could be decreased under magnetic influence and thereby biomass accumulation could be more favored. But this is not always true for all plant species because P. pereskiifolia in this experiment, showed no changes in both photosynthetic rate and biomass accumulation. Leaf nitrogen and chlorophyll contents were enhanced significantly in most plant species under influence of SMF. Chlorophyll a/b ratio also increased by SMF. Although there might be a limitation depending on plant species, these results suggest that long-term exposure to SMF might allow plant to have an enhanced acclimation capacity against environmental fluctuations and optimal application of SMF could increase the practical use of indoor plants such as an attempt to improve indoor air quality.
Journal of The Korean Society of Grassland and Forage Science
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v.6
no.3
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pp.131-137
/
1986
Korean lawn, Zoysia japonica STEUD, is the type of a perennial warm-season lawn grass that has a naturally green period during 5 months from May to September. This study was carried out to detect the effects of N-P-K fertilization, regrowth capacity after mowing and chlorophyll contents on prolongation of the green period in Zoysia japonica grasslands. The results obtained are summarized as follows: 1. The effects of nitrogen fertilizer application on Zoysia japonica grasslands were affected on regrowth capacity after mowing, green color of leaf canopies and chlorophyll contents, respectively. 2. A the experimental plots which applied 45g/$m^2$/10 weeks of $N_3$ fertilizer, there were high significant effects on prolongation of the green period and the plots of phosphorus and potassium had additive effects. 3. Prolongation of the green period to the last ten days of October was obviously possible by slow degrees in proportion to increment of N-P-K fertilization. 4. However, it is interpreted that more prolongation of the green period is impossible with N-P-K fertilization and irrigation treatments, unless the intermediate turfgrasses between $C_3$ and $C_4$ plants must be found and bred.
This paper was performed to study the nature of varietal resistance of some Korean-new rice cultivars to the brown planthopper (BPH), Nilaparvata lugens ($ST{\AA}L$). The rice cultivars tested were Cheongcheong, Gaya, Hangangchal, Samgang, Nampoong and Yeongpoong which have been reported as having resistant genes for the BPH. The check varieties were Jinheung, Sangpoong and Chucheong for susceptible and IR-36 for resistant. The factors studied were referred to the seedling responses, preference in feeding and oviposition of BPH, antibiosis (nymphal development, adult emergence and sex ratio, adult body weight, population build-up, feeding amount and amylase activity), and chemical composition (inorganic components, chlorophyll contents, cell wall components, amino acids and esterase isozymes) of leaf- sheath and/or roots of rice plants. In conclusion, the natures of varietal resistance of rice cultivars to the BPH were not only correlated with the resistant reaction of rice plant, but also they were related with the non preference in feeding and oviposition and those resistant cultivars had the antibiotic effects to the insects. Their antibiotic effects of rice cultivars to the BPH would be related with some of the chemical components of rice plants, such as the contents of magnesium oxide (MgO), and chlorophyll and the different esterase isozymes.
A pot experiment was carried out using Seomjinbyeo to investigate the effects of some sulfur-containing substances on the growth of rice plants and on soil characteristics. The results are summarized as follows: 1. Soil pH in plots given the sulfur treatments was lower than that in the urea plot during the growing stages. Soil pH in plots given the mineral sulfur treatments was higher than that in the ammonium sulfate plot at the early growth stage of rice. However, as the stage of rice growth progressed, soil pH became similar. After the experiment, soil pH was higher, but the sulfur content of soil was lower in the urea plot compared with that in other sulfur plots. After the experiment, the sulfur content of soil in the ammonium sulfate plot was the lowest among the plots given the sulfur treatments. 2. Plant heights and the number of tillers of the rice plants in plots given the sulfur treatments were higher than those in the urea plot, specially in the number of tillers of the rice plants. 3. Nitrogen and sulfur contents of the tops of rice plants in plots given the sulfur treatments were slightly higher than those in the urea plot. nitrogen and sulfur contents in the ammonium sulfate plot were higher than those in plots given the mineral sulfur treatments at the early growth stage of rice. However, as the stage of rice growth progressed, those in plots given the mineral sulfur treatments were higher. 4. The chlorophyll content in the leaf blade at heading stage in plots given the gypsume and given the mineral sulfur treatments was significantly higher than that in the urea plot. The N/S ratio was negatively correlated with the chlorophyll content.
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