• Korean Journal of Plant Tissue Culture
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• v.27 no.6
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• pp.485-489
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• 2000

The patterns and contents of ginsenosides were examined in normal root parts and hairy root lines of Panax ginseng C. A. Meyer. Ginsenoside-Rb$_1$, -Rb$_2$, -Rc, -Rd, -Re, -Rf, -Rg$_1$, -Rg$_2$ were detected in normal roots and hairy roots of ginseng. The patterns and contents of ginsenosides in that were very difference each other. The contents of total ginsenoside of hairy root (KGHR-1) was 17.42 mg/g dry wt, it's highest compared to others. Ginsenoside contents of hairy root (KGHR-1) was higher on ginsenoside-Rd, Rg$_1$, KGHR-5 was higher on ginsenoside-Rb$_1$, Rg$_1$, and KGHR-8 was higher on ginsenoside-Rd, Re than others. The contents of total ginsenosides on 6 years old ginseng cultured in the field were high in the order of main root, lateral root and fine roots, and content of ginsenosides in fine roots was 3.2 times higher than that in main root. The ratio of ginsenoside-Rg$_1$to total ginsenosides were about 3.43%, 8.68% and 14.18% respectively on fine root, lateral root and main root, it's very lower than that in hairy roots. It is suggested that specific ginsenosides can be produce in cultures of ginseng hairy roots.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.6
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• pp.472-476
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• 2004

Lateral roots formed on mungbean sprouts should lower their quality. The study was carried out to clarify the effects of aeration periods (AP; 1, 2, 3, and 4 hours) and temperature (AT; 20, 30, and $40^{\circ}C$) after 5 hour seed imbibition into 50 ppm benzyladenopurine (BA) solution on growth and morphological characters of mungbean (cv. Keumseongnogdu, Owoolnogdu and Zhong Lu 1) sprouts. On the 6th day, the mungbean sprouts were sorted by 4 categories on the base of hypocotyl lengths; > 7cm, 4 to 7cm, < 4cm, and non-germination to calculate their composition rates, number of lateral roots, lengths of hypocotyl and root, diameters at middle and upper parts of hypocotyl, fraction fresh and dry weights were measured. AP more affected growth of the cultivars than AT showing little effect on them. In the composition rate of the above 4 categories, cv. Zhong Lu 1 had the highest rate in longer than 7cm but nearly the same rate in AP treatments. Rates of longer than 4cm hypocotyls in cv. Keumseongnogdu, Owoolnogdu were increased with longer AP but their rates of shorter than 4cm showed reverse response to the former. Formation rate and number of lateral roots per sprout were decreased with longer AP, showing more severe decrement when delayed 3 to 4 hour AP. Upper part of hypocotyls and roots were more thickened and shortened in longer AP, respectively. Total fresh weights had no significant difference between AP treatments while hypocotyl fresh weights were increased with longer AP. It was concluded that in mungbean sprout culture aeration from BA treatment to the first watering permitted at least 4 hours.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.2
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• pp.147-154
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• 1989

The experiments were carried out to compare the growth characteristics of excised seminal roots in Japonica type, Indica- Japonica hybrids and its parents. Based on the modified culture medium by Kawata et al., several levels of pH. sucrose and casamino acid were varied in the culture media. The excised root length at 27$^{\circ}C$ incubator was little different between Japonica type and Indica-Japonica hybrids, but at 30$^{\circ}C$ incubator the excised root elongation of Indica-Japonica hybrids was much better than that of Japonica type. The dry matter weight of excised root in Indica- Japonica hybrids was significantly heavier than in Japonica type at both 27 and 30$^{\circ}C$ incubators. The difference of dry root weight between Japonica type and Indica- Japonica hybrids was caused from the number of lateral roots, especially the root numbers of 25 cm length. The main and lateral seminal root growth of Japonica type grew best at the lot of 0.2 percent casamino acid. However, the lots of 0 and 0.2 percent casamino acid showed good results in the main and lateral excised root growth. respectively of Indica-Japonica hydrids. The growth of excised roots was better in Indica-Japonica hybrids than in Japonica type at even various levels of pH, sucrose and casamino acid. Between the parents of Indica - Japonica hybrids, the excised root growth of Indica type was more predominant than that of Japonica type, and the effect of casamino acid on the excised root growth in the formar was similar to that of Indica type. This result indicated that the growth characteristics of excised seminal roots in Indica Japonica hybrids were derived from the parent, Indica type.

• Korean Journal of Medicinal Crop Science
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• v.23 no.4
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• pp.292-297
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• 2015

This study was carried out to have the basic and applied informations relating to develop the cultivation methods and to increase the productivity and quality of ginseng. 1 to 6 year old ginsengs of Jakyung cultivar were cultivated and the content and synthetic amount of carbohydrates were investigated with different plant tissues, growth stages, and years old. The concentration of total carbohydrates at six year old ginseng including water soluble and water insoluble carbohydrates was about 18.9%, 42.9%, and 43,6% in leaves, tap roots, and lateral roots, respectively. Water soluble carbohydrate of tap and lateral roots was slightly decreased from August until September, and then increased on November, whereas its water insoluble carbohydrate was increased from August to September and then decreased on November. Comparing with the content of carbohydrates of 1 to 6 year old ginsengs, it was continuously increased from one year old ginseng until five year old ginseng, however it was not increased much in six year old ginseng. The highest content of carbohydrates was at five year-old in all tissues of ginseng. Water soluble and water insoluble carbohydrates were significantly shown different in leaves, stems, tap roots, and lateral root at different growth stages and with different years old. The content of water soluble carbohydrate in the leaves was remarkedly higher compared to that of water insoluble carbohydrate, while in the root the content of water insoluble carbohydrate was clearly higher compared to the water soluble carbohydrate. Comparing with the synthetic amount of carbohydrates, water soluble carbohydrates was higher in the shoot than that in the root, whereas water-insoluble carbohydrates higher in the root than that in the shoot. Carbohydrates which would be utilized in ginseng tissues for short and long-term periods as major energy were appeared differently in between shoot and root, with different growth stages, and years old.

• Korean Journal of Environmental Agriculture
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• v.28 no.4
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• pp.447-452
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• 2009

A bacterium, which was named to be Bacillus cereus A-139, secreting auxin was isolated from the east coast sand dunes in Korea. The secretion of auxin was confirmed by HPLC. When cultured in LB broth, Bacillus cereus A-139 produced $16.12\;{\mu}$g/mL auxin after 8 days in LB broth. Bacillus cereus A-139 produced $49\;{\mu}$g/mL auxin and $162.6\;{\mu}$g/mL by the addition of 2% tryptone and 0.1% tryptophan, respectively. The root growth of Arabidopsis thaliana was retarded by Bacillus cereus A-139 culture broth up to 57% but the formation of lateral roots was increased up to almost twice after 4 days incubation. Also the formation of lateral roots of mung bean was increased up to 57% after 10 days incubation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.4
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• pp.292-296
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• 2002

Soybean seeds were treated with blue, red and far-red lights for 0, 6, 12, and 24 hours during 24-hour imbibition before culture for 6 days. The soybean sprouts raised were classified by their hypocotyl lengths; normal (＞4cm), abnormal (＜4cm) and non-germination, and their lateral roots, hypocotyl diameters and component dry weights were measured. Red light treatment and dark imbibition reduced the abnormal soybean sprouts more than far-red and blue light treatments, meaning that the former treatments produced more commercial sprouts. The lateral roots were more formed in blue light and dark imbibition than the other light treatments, but were completely blocked by any light treatment lasted during the whole imbibition. Although any light quality treatment did not influence their primary root lengths, blue light one lengthened the hypocotyl more than the others treated during the imbibition, and far-red light enlarged its diameter. Despite this morphological change, component, total or economic yield was not significantly different among the light quality treatments during the imbibition.

• Restorative Dentistry and Endodontics
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• v.4 no.1
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• pp.23-27
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• 1978

Fifty two mandibular permanent canines were chosen to study the anatomy of the root canal. The experimental teeth were injected with China ink, decalcified, cleared and observed to investigate the number of root canals, frequency and location of lateral canals, the location of apical foramens, the frequency of apical deltas, and the curvature of root canals. The results were as follows: 1) all of the experimental teeth demonstrated single canal. 2) of the 52 canals, 9.6% of the canals were found to have lateral canals and the locations of the lateral canals were in the apical third of the roots 3) Thirty three apical foramens were located laterally on the root apices and nineteen foramens were located on the centers of the root apices. 4) 37 canals showed straight curvature, 12 canals distal curvature. 3 canals labial curvature.

• Korean Journal of Medicinal Crop Science
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• v.17 no.6
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• pp.452-457
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• 2009

This study was carried out to investigate the correlation between root diameter and ginsenoside composition of Panax ginseng C. A. Meyer cultivar Yunpoong. Dry matter ratio of main root was a little higher than that of lateral root and fine root, and that was higher by the increase of root diameter in the same root parts. Total ginsenosides composition of main and lateral roots increased by the decrease of root diameter, especially in lateral root. Similar resulted in fine root, but there was no significant difference where root diameter was below 2.5 mm. Except for ginsenoside-$Rg_1$, other ginsenosides component, PDs, PTs and total ginsenosides had highly negative correlation with the root diameter within whole root, main root+lateral root and lateral root+fine root, while $Rg_1$ had positive correlation with the root diameter.

• Journal of Environmental Science International
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• v.1 no.2
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• pp.145-155
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• 1992

The effect of various supplies of lead singly and in combination with aluminium on growth and chlorophyll biosynthesis was investigated in 7-day-old Vigna anguluris seedlings. Expose to 50 $\mu$N Pb or more drastically reduced root elongation rate. Significant depressions in root growth was observed within 1 day and no recovery of growth was seen over the duration of treatment period. Root elongation decreased depending on the Pb concentrations. Root growth inhibition was stronger than shoot growth inhibition. The initiation of lateral roots appeared to be more sensitive to Pb than the growth of main roots. Inhibition of root and shoot elongation by Pb was lessened by combined exposure of Pb and Al, suggesting that the presence of AA reverse the inhibitory effect of Pb alone. With the histochemical sodium rhodizonate method the rate of Pb uptake was dependent on the Pb concentration and exposure time of the roots to Pb salts. Pb was first deposited on the root surface and then translocated radially in the root cap cells. During a longer Pb administration (up to 72 h) Pb penetration was nonuniform, with accumulation within the cortex or endodermis. There was drastic reduction in chlorophyll content by Pb. The Pb inhibition of chlorophyll synthesis was concentration dependent. 5-Aminolevulinic acid dehydratase (ALAD) activity exhibited distinct inhibition from control. Reduction in chlorophyll content was accompanied by proportional changes in ALAD activity. Chlorophyll content and ALAD activity were less affected by combined exposure of Pb and Al, suggesting that Al has a protective effect against the inhibiting action of Pb on photosynthetic activity.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.1 no.2
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• pp.145-155
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• 1997

The effect of various supplies of lead singly and in combination with aluminium on growth and chlorophyll biosynthesis was investigated in 7-day-old Vigna angularis seedlings. Expose to 50 uM Pb or more drastically reduced root elongation rate. Significant depressions in root growth was observed within 1 day and no recovery of growth was seen over the duration of treatment period. Root elongation decreased depending on the Pb concentrations. Root growth inhibition was stronger than shoot growth inhibition. The initiation of lateral roots appeared to be more sensitive to Pb than the growth of main roots. Inhibition of root and shoot elongation by Pb was lessened by combined exposure of Pb and Al, suggesting that the presence of Al reverse the inhibitory effect of Pb alone. With the histochemical sodium rhodizonate method the rate of Pb uptake was dependent on the Pb concentration and exposure time of the roots to Pb salts. Pb was first deposited on the root surface and then translocated radially in the root cap cells. During a longer Pb administration (up to 72 h) Pb penetration was nonuniform, with accumulation within the cortex or endodermis. There was drastic reduction in chlorophyll content by Pb. The Pb inhibition of chlorophyll synthesis was concentration dependent. $\delta-Aminolevulinic$ acid dehydratase (ALAD) activity exhibited distinct inhibition from control. Reduction in chlorophyll content was accompanied by proportional changes in ALAD activity. Chlorophyll content and ALAD activity were less affected by combined exposure of Pb and Al, suggesting that Al has a protective effect against the inhibiting action of Pb on photosynthetic activity.