• Ocean Systems Engineering
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• v.1 no.3
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• pp.195-205
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• 2011

A method for calibrating a laser profiling system for seafloor micro-topography measurements is described. The system consists of a digital camera and an arrangement of six red lasers that are mounted as a unit on a remotely operated vehicle (ROV). The lasers project as parallel planes onto the seafloor, creating profiles of the local topography that are interpreted from the digital camera image. The goal of the calibration was to determine the plane equations for the six lasers relative to the camera. This was accomplished in two stages. First, distortions in the digital image were corrected using an interpolation method based on a virtual pinhole camera model. Then, the laser planes were determined according to their intersections with a calibration target. The position and orientation of the target were obtained by a registration process. The selection of the target shape and size was found to be critical to a successful calibration at sea, due to the limitations in the manoeuvrability of the ROV.

• BMB Reports
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• v.41 no.9
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• pp.626-634
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• 2008

Novel cancer biomarkers are required to achieve early diagnosis and optimized therapy for individual patients. Cancer is a disease of the genome, and tumor tissues are a rich source of cancer biomarkers as they contain the functional translation of the genome, namely the proteome. Investigation of the tumor tissue proteome allows the identification of proteomic signatures corresponding to clinico-pathological parameters, and individual proteins in such signatures will be good biomarker candidates. Tumor tissues are also a rich source for plasma biomarkers, because proteins released from tumor tissues may be more cancer specific than those from non-tumor cells. Two-dimensional difference gel electrophoresis (2D-DIGE) with novel ultra high sensitive fluorescent dyes (CyDye DIGE Fluor satulation dye) enables the efficient protein expression profiling of laser-microdissected tissue samples. The combined use of laser microdissection allows accurate proteomic profiling of specific cells in tumor tissues. To develop clinical applications using the identified biomarkers, collaboration between research scientists, clinicians and diagnostic companies is essential, particularly in the early phases of the biomarker development projects. The proteomics modalities currently available have the potential to lead to the development of clinical applications, and channeling the wealth of produced information towards concrete and specific clinical purposes is urgent.

• Construction Engineering and Management
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• v.4 no.1 s.13
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• pp.48-54
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• 2003

An automated system for scanning and characterizing unbound aggregates, called the 'Laser-based Aggregate Scanning System'(LASS), has been developed at the University of Texas at Austin. The system uses a laser profiler to acquire and analyze true three-dimensional data on aggregate particles to measure various morphological properties. Tests have demonstrated that the system can rapidly and accurately measure grain size distribution and dimensional ratios, and can objectively quantify particle shape, angularity, and texture in a size invariant manner. In its present state of development, the LASS machine is a first-generation, laboratory testing device. With additional development, this technology is expected to provide high-quality, detailed information for laboratory and on-line quality control during aggregate production.

• Proceedings of the KIEE Conference
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• 1989.11a
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• pp.79-82
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• 1989

We studied the epitaxial growth of GaAs/GaAs and GaAs/Si by Laser CVD with 193nm ArF pulsed excimer laser. The source gases of TMGa and AsC13 or TMGa-TMAs adducts are mixed with H2, and photolyzed above the substrate which is heated up to around 300$^{\circ}C$. Then the photolyzed atoms are deposited on the silicon or GaAs substrate. The deposited films are analyzed with ESKA depth profiling and X-ray differaction method, which shows that the films on Si and GaAs are stoichiometric and crystalized at such a low temperature. We show a clear evidence for the epitaxial growth of GaAs on Si or GaAs on GaAs at low temperature by excimer laser CVD.

• The Zoological Society Korea : Newsletter
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• v.18 no.2
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• pp.21-25
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• 2001

대부분의 조직은 여러 가지 세포가 모여서 이루어지기 때문에 그 중의 어떤 특정세포에서 발현하는 물질을 분석하려면 조직을 이루고 있는 각각의 세포를 분리해내야 한다. 이렇게 순수하게 세포를 분리해내는 기술 중의 하나가 Laser Captured Microdissection (LCM)이 다. LCM의 개발로 기존에 사용되던 방법에 비하여 빠르고 간편하면서, 매우 정확하게 원하는 세포를 순수 분리해서 그 세포의 분자생물학적 또는 생화학적인 분석을 할 수 있게 되었다. LCM은 현미경으로 조직절편을 관찰하면서 원하는 세포를 낮은 에너지의 laser를 사용하여 도려내는 방법으로 조직절편 이외에도 도말된 혈액이나 자궁경부 조직, 그리고 배양된 세포를 cytocentrifugation한 후에 원하는 세포를 포획 할 수도 있다. LCM을 이용한 연구는 여러 분야에서 다양하게 진행되고 있으며, 특히 같은 조직 내에 존재하는 정상세포와 전이중인 세포, 그리고 암세포를 구분해 냄으로써 암의 전이기전 및 병인 연구에 매우 큰 공헌을 하고 있다. 이렇게 분리된 세포는 RT-PCR, LOH (loss of heterozygosity), microsatellite instability, differential gene profiling, cDNA microarray, Western blot, 2D PAGE protein analysis 등의 기법을 접목하여 연구하게 된다. 본 논단을 통하여 1996년 개발된 LCM의 원리와 이제까지 LCM을 이용한 연구 성과를 살펴보고자 한다.

• Proceedings of the Korean Vacuum Society Conference
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• 2016.02a
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• pp.384.1-384.1
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• 2016

We reported that amorphous silicon (a-Si) thin film provide sample plate exhibiting a multimodality to measure biomolecules by secondary ion mass spectrometry (SIMS) and laser desorption/ionization mass spectrometry (LDI-MS). Kim et al.1 reported that a-Si thin film were suitable to detect small molecules such as drugs and peptides by SIMS and LDI-MS. Recently, bacterial identification has been required in many fields such as food analysis, veterinary science, ecology, agriculture, and so on.2 Mass spectrometry is emerging for identifying and profiling microbiology samples from its advantageous characters of label-free and shot-time analysis. Five species of bacteria - S. aureus, G. glutamicum, B. kurstaki, B. sphaericus, and B. licheniformis - were sampled for MS analysis without lipid extraction in sample preparation steps. The samples were loaded onto the a-Si thin film with a thickness of 100 nm which did not only considered laser-beam penetration but also surface homogeneity. Mass spectra were recorded in both positive and negative ionization modes for more analytical information. High reproducibility and sensitivity of mass spectra were demonstrated in a mass range up to mass-to-charge ratio(m/z) 1200 by applying the a-Si thin film in mentioned above MS. Principle component analysis (PCA) - a popular statistical analysis widely used in data processing was employed to differentiate between five bacterial species. The PCA results verified that each bacterial species were readily distinguished and differentiated effectively from our MS approach. It shows a new opportunity to rapid bacterial profiling and identification in clinical microbiology. More details will be discussed in the presentation.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.21 no.5
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• pp.135-140
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• 2021

In this paper, we propose an on-line mechanism that simultaneously detects cracks and profiling pavement blocks to detect the displacement of ground surface adjacent to the excavation in the urban area. The proposed method utilizes a 2D laser to profile the information about pavement blocks including the depth and distance among them. In particular, it is designed to enable the detection of cracks and portholes at runtime. For the experiment, real data was collected through Gocator, and trainng was carried out using Faster R-CNN. The performance evaluation shows that our detection precision and recall are more than 90% and the pavement blocks are profiled at the same time. Our proposed mechanism can be used for monitoring management to quantitatively detect the level of excavation risk before a large-scale ground collapse occurs.

• BMB Reports
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• v.48 no.7
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• pp.388-394
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• 2015

The brain is an organ that consists of various cell types. As our knowledge of the structure and function of the brain progresses, cell type-specific research is gaining importance. Together with advances in sequencing technology and bioinformatics, cell type-specific transcriptome studies are providing important insights into brain cell function. In this review, we discuss 3 different cell type-specific transcriptome analyses i.e., Laser Capture Microdissection (LCM), Translating Ribosome Affinity Purification (TRAP)/RiboTag, and single cell RNA-Seq, that are widely used in the field of neuroscience. [BMB Reports 2015; 48(7): 388-394]

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.7 no.4
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• pp.147-150
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• 2014

In this paper, pulse current type power supply for laser diode dirver is discussed. High speed high power laser has been using widely because it becomes using on medical or manufacturing equipment, but researches have not being occurred actively. Current profiling method is proposed for improved lifetime expectancy of laser diode(LD). The current waveform of driving LD is most important factor of the performance and lifetime of LD. The proposed method improves shape and stability of current waveforms, thus will effect right direction of lifetime expectation.

• Journal of Electrical Engineering and Technology
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• v.13 no.1
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• pp.438-444
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• 2018

Structured light vision system has been widely used in 3D surface profiling. Usually, it is composed of a camera and a laser which projects a line on the target. Calibration is necessary to acquire 3D information using structured light stripe vision system. Conventional calibration algorithms have found the pose of the camera and the equation of the stripe plane of the laser under the same coordinate system of the camera. Therefore, the 3D reconstruction is only possible under the camera frame. In most cases, this is sufficient to fulfill given tasks. However, they require multiple images which are acquired under different poses for calibration. In this paper, we propose a calibration algorithm that could work by using just one shot. Also, proposed algorithm could give 3D reconstruction under both the camera and laser frame. This would be done by using newly designed calibration structure which has multiple vertical planes on the ground plane. The ability to have 3D reconstruction under both the camera and laser frame would give more flexibility for its applications. Also, proposed algorithm gives an improvement in the accuracy of 3D reconstruction.