• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.32 no.1
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• pp.36-41
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• 2006

Objective. The objective of this study was to examine the hypothesis that inflammatory synovial fluid from TMJ internal derangement initiates a transient increase in intracellular calcium concentration ([$Ca^{2+}$]i) in chondrocytes and the induced Ca2+ signaling affects iNOS/COX-2 gene expression patterns following exposure to inflamed synovial fluid. Materials and Methods. Two female adult patients with symptoms of TMD who agreed to participate in the study were selected for this study. Immortalized human juvenile costal chondrocyte C-28/I2 was grown to 80% confluency and synovial fluids from two patients were added respectively to culture media for 24 hours at the concentration of 100ng/10ml. Confocal laser scanning microscope (CLSM) was used to examine changes of intracellular calcium concentration ([$Ca^{2+}$]i). RT-PCR was performed to identify the expression profile of IL-1${\alpha}$, iNOS, COX-2. Results. Increased [$Ca^{2+}$]i was observed in chondrocytes subjected to inflamed synovial fluid compared to control cultures and in respective cultures exposed to inflamed synovial fluids from each patient, IL-1${\beta}$, COX-2 mRNA were detected. However, in neither case iNOS mRNA was expressed. IL-1${\alpha}$, COX-2, and iNOS mRNA were expressed in control culture. Conclusion. Our results show that immortalized chondrocytes cultured with inflamed synovial fluids from patients diagnosed as disc displacement without reduction and limitation in mouth opening showed increased calcium concentration and expression of COX-2 while inhibiting the production of iNOS, which in turn could adversely affect the chondrocytes in at least short term by hindering physiologic role of NO against inflammatory cascades. These findings suggest that inflamed synovial fluid may differentially regulate the transcriptomes of relevant inflammatory mediators, especially iNOS/COX-2 axis in chondrocytes through adjusting calcium transients.

• JSTS:Journal of Semiconductor Technology and Science
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• v.1 no.1
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• pp.84-93
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• 2001

An electromagnetic micro x-y stage for probe-based data storage (PDS) has been fabricated. The x-y stage consists of a silicon body inside which planar copper coils are embedded, a glass substrate bonded to the silicon body, and eight permanent magnets. The dimensions of flexures and copper coils were determined to yield $100{\;}\mu\textrm{m}$ in x and y directions under 50 mA of supplied current and to have 440 Hz of natural frequency. For the application to PDS devices, electromagnetic stage should have flat top surface for the prevention of its interference with multi-probe array, and have coils with low resistance for low power consumption. In order to satisfy these design criteria, conducting planar copper coils have been electroplated within silicon trenches which have high aspect ratio ($5{\;}\mu\textrm{m}$in width and $30{\;}\mu\textrm{m}$in depth). Silicon flexures with a height of $250{\;}\mu\textrm{m}$ were fabricated by using inductively coupled plasma reactive ion etching (ICP-RIE). The characteristics of a fabricated electromagnetic stage were measured by using laser doppler vibrometer (LDV) and dynamic signal analyzer (DSA). The DC gain was $0.16{\;}\mu\textrm{m}/mA$ and the maximum displacement was $42{\;}\mu\textrm{m}$ at a current of 180 mA. The measured natural frequency of the lowest mode was 325 Hz. Compared with the designed values, the lower natural frequency and DC gain of the fabricated device are due to the reverse-tapered ICP-RIE process and the incomplete assembly of the upper-sided permanent magnets for LDV measurements.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2017.04a
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• pp.141-141
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• 2017

장미과(Rosaceae)에 속하는 딸기(Fragaria ananassa Duch.)는 비타민 C가 풍부하고 독특한 향기를 갖는 과채류로서 겨울에서 봄까지의 기간 동안 대부분 생식으로 소비되고 있다. 국내에서 재배되는 품종으로는 설향, 매향, 장희 등이 있으며 품종에 따라 성분과 함량이 다양하지만 일반적으로 유기산이 많아서 신맛과 단맛이 조화로운 특징이 있다. 소비자들이 딸기를 구입할 때 딸기가 포장된 상자에 모양이 일정하고 붉은 색상이 선명한 딸기에 호감을 갖게 된다. 딸기는 품종에 따라 기준이 되는 모양이 다르기 때문에 숙련된 선별사에 의해서 대부분 육안으로 선별되고 있는 실정이다. 하지만 개인적인 선별 능력의 차이와 주관적인 판단으로 인해 규격을 벗어난 딸기가 혼입되어 전체적인 품질 등급을 떨어뜨리는 경우가 종종 발생하기도 한다. 따라서 본 연구에서는 품종별로 기준이 되는 표준 형상과 비정상적인 모양의 기형 딸기를 객관적으로 판별하여 선별할 수 있는 영상 시스템을 구축하기 위해 수행되었으며 표준이 되는 딸기의 3차원 형상을 구축하기 위해 2차원 레이저 변위 센서를 이용하여 딸기의 입체 영상을 구축하고자 하였다. 실험을 위해 사용된 딸기는 시중에서 구입한 설향 품종이었으며 2차원 레이저 변위 센서는 라인 스캔 방식으로 1회 프로파일 스캔에 1,280개의 데이터 포인터를 획득할 수 있으며 분해능은 0.095~0.17 mm이었다. 상부에 부착된 2차원 레이저 변위 센서와 하부에 놓인 딸기의 거리는 100 mm였다. 획득한 딸기의 2차원 영상은 높이 차이를 이용하여 색상 농도로 표현하였으며 이 영상을 다시 3차원 영상으로 구축하였다.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.24 no.1
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• pp.21-28
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• 2000

Although commercial PIV systems have been widely used for the non-intrusive velocity field measurement of fluid flows, they are still under development and have considerable room for improvement. In this study, a single-frame double-exposure PIV system using a high-resolution CCD camera was developed. A pulsed Nd:Yag laser and high-resolution CCD camera were synchronized by a home-made control circuit. In order to resolve the directional ambiguity problem encountered in the single-frame PIV technique, the second particle image was genuinely shifted in the CCD sensor array during the time interval dt. The velocity vector field was determined by calculating the displacement vector at each interrogation window using cross-correlation with 50% overlapping. In order to check the effect of spatial resolution of CCD camera on the accuracy of PIV velocity field measurement, the developed PIV system with three different resolution modes of the CCD camera (512 ${\times}$ 512, lK ${\times}$ IK, 2K ${\times}$ 2K) was applied to a turbulent flow which simulate the Zn plating process of a steel strip. The experimental model consists of a snout and a moving belt. Aluminum flakes about $1{\mu}m$ diameter were used as scattering particles for the liquid flow in the zinc pot and the gas flow above the zinc surface was seeded with atomized olive oil with an average diameter of 1-$3{\mu}m$. Velocity field measurements were carried out at the strip speed $V_s$=1.0 m/s. The 2K ${\times}$ 2K high-resolution PIV technique was significantly superior compared to the smaller pixel resolution PIV system. For the cases of 512 ${\times}$ 512 and 1K ${\times}$ 1K pixel resolution PIV system, it was difficult to get accurate flow structure of viscous flow near the wall and small vortex structure in the region of large velocity gradient.

• International Journal of Control, Automation, and Systems
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• v.3 no.3
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• pp.477-485
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• 2005

This paper is concerned with the development of a micro tensile testing machine for optically functional materials such as single or poly crystalline silicon and nickel film. This micro tensile tester has been developed for testing various types of materials and dimensions. PZT type actuation is utilized for precise displacement control. The specifications of the PZT actuated micro tensile testers developed are as follows: the volumetric size of the tester is desktop type of 710mm' 200mm' 270mm; the maximum load capacity and the load resolution in this system are IKgf and 0.0152mgf respectively and; the full stroke and the stoke resolution of the PZT actuator are $1000{\mu}m$ and 10nm respectively. Special automatic specimen installing and setting equipment is applied in order to prevent unexpected deformation and misalignment of specimens during handling of specimens for testing. Nonlinearity of the PZT actuator is compensated to linear control input by an inverse compensation method that is proposed in this paper. The strain data is obtained by ISDG method that uses the laser interference phenomenon. To test the reliance of this micro tensile testing machine, a $200{\mu}m$ thickness nickel thin film and SCS (Single Crystalline Silicon) material that is made with the MEMS fabrication process are used.

• Journal of the Korean Society for Precision Engineering
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• v.27 no.1
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• pp.125-133
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• 2010

A versatile micro-robotic platform for micro/nano scale assembly has been demanded in a variety of application areas such as micro-biology and nanotechnology. In the near future, a flexible and compact platform could be effectively used in a scanning electron microscope chamber. We are developing a platform that consists of miniature mobile robots and a compact positioning stage with multi degree-of-freedom. This paper presents the design and the implementation of a low-cost and compact multi degree of freedom position sensor that is capable of measuring absolute translational and rotational displacement. The proposed sensor is implemented by using a CMOS type image sensor and a target with specific hole patterns. Experimental design based on statistics was applied to finding optimal design of the target. Efficient algorithms for image processing and absolute position decoding are discussed. Simple calibration to eliminate the influence of inaccuracy of the fabricated target on the measuring performance also presented. The developed sensor was characterized by using a laser interferometer. It can be concluded that the sensor system has submicron resolution and accuracy of ${\pm}4{\mu}m$ over full travel range. The proposed vision-based sensor is cost-effective and used as a compact feedback device for implementation of a micro robotic platform.

• Journal of the Korean Society for Nondestructive Testing
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• v.13 no.2
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• pp.20-30
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• 1993

Electric Speckle Pattern Interferometry (ESPI) using a CW-laser, a video system and an image processor was applied to the thermal coefficient measurements on free thermal expansions at high temperatures : ESPI provides the distribution of in-plane displacement resolved in a preselected direction. ESPI retains the merits of little or no surface preparation, no contact with the surface and the real-time presentation of interference fringes. Appling ESPI at high temperatures, several problem which caused the reduction of fringe visibility were encountered. The problem on the turbulence in the hot air surrounding high temperature objects will be solved by using a vacuum chamber. The background radiations from the objects were suppressed considerably by an interference filter. The problem on the oxidation of the object surface could't be solved. The interference fringe, whose spacings were calculated by FFT to avoid human error, were observable up to $800^{\circ}C$. The results measured by ESPI were nearly equal to the data which have already been published, up to about $800^{\circ}C$.

• Journal of the Korean Society for Nondestructive Testing
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• v.22 no.4
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• pp.393-401
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• 2002

Pipelines in power plants, nuclear facilities and chemical industries are often affected by corrosion effects. It is important to inspect the internal defects in pipelines in oder to guarantee safe operational condition. We have taken relatively much time, cost and manpower to use conventional NDT methods because these methods are contact measuring methods. In this paper, we used digital shearography, a laser-based optical method which allows full-field measurement of surface displacement derivatives. This method has many advantages in practical use, such as low sensitivity to environmental noise, simple optical configuration and real time measurement. The experiment was performed with pressure vessels which has different internal cracks and detected internal cracks in the pressure vessels at a real time using phase shifting method.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.25 no.1
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• pp.13-23
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• 2015

The paper experimentally deals with the radial in-plane vibration characteristics of disk-shaped piezoelectric transducers. The radial in-plane motion, which is induced due to Poisson's ratio in the piezoelectric disk polarized in the thickness direction, was measured by using an in-plane laser vibrometer, and the natural frequencies were measured by using an impedance analyzer. The experimental results have been compared with theoretical predictions obtained by simplified theoretical and finite-element analyses. It appears that the fundamental mode of a piezoelectric disk transducer is a radial mode and its radial displacement distribution from the center to the perimeter is not monotonic but shows maximum slightly apart from the perimeter. The theoretically-calculated fundamental frequencies agree well with the finite-element results for small thickness-to-diameter ratio, and they are accurate within 7 % error for the ratio up to 0.4.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.25 no.12
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• pp.807-814
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• 2015

A control rod drive mechanism(CRDM) is a reactor regulating system, which inserts, withdraws or maintains a control rod containing a neutron absorbing material within a reactor core to control the reactivity of the core. The top-mounted CRDM for Jordan Research and Training Reactor(JRTR) with 5 MW power has been designed and fabricated based on the HANARO's experience through KAERI and DAEWOO consortium project. This paper describes the performance qualification test results to demonstrate the operability of a prototype and four production CRDMs during the reactor lifetime. The driving performance, the drop performance and the endurance tests for CRDM are carried out at a test rig simulating the actual reactor conditions. A vibration of internal components due to the coolant flow is also measured using a laser vibrometer. As a result, the CRDMs are driven having a good driving performance without a malfunction between command and output signals for the stepping motor. Also, the pure drop time and the impact acceleration are within 0.72 s and 4.2 g to meet the design requirements, and the vibrational displacement of control rod is measured as maximum $5.2{\mu}m$.