• 음성과학
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• 제15권3호
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• pp.159-173
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• 2008

The purpose of this study was to use fiber-optic nasolaryngoscope to find out differences in laryngeal behavior between persons who stutter(PS) and those who do not stutter(NS) upon their utterance. To meet the goal above, this study took 5 NS and 5 PS respectively as a part of sampling, so that they were all asked to join an experiment hereof. As a result, this study came to the following findings: First, there was not any significant difference in laryngeal behavior of uttering spoken languages between stuttering group and control. Second, there were some differences in laryngeal behavior of repetition and prolongation, which were a sort of disfluency revealed in the utterance of nonfluent spoken languages between stuttering group and control. Third, as reported by prior studies, it was found that there were differences in laryngeal abehavior of stutterer group's nonfluent spoken languages depending upon stuttering types. In this study, a variety of laryngeal behavior unreported in prior studies could be found. In addition, it was notable that stutterers showed different laryngeal behavior depending on their personal stuttering types. On block condition, Subject 1 showed laryngeal behavior of fAB, INT and fAD; Subject 2 showed laryngeal behavior of fAB, fAD and rAD; Subject 3 showed laryngeal behavior of fAD and rAD; Subject 4 showed only laryngeal behavior of fAD; and Subejct 5 showed laryngeal behavior of fAB, fAD and rAD. Summing up, these findings imply that when stutterers utter nonfluent words, they may reveal a variety of laryngeal behavior depending on their personal stuttering types. Moreover, it is found that there are more or less differences in the utterance of nonfluent spoken languages between NS and stuttering ones. In particular, it is interesting that one common trait of nonfluent spoken languages uttered by PS is evidently excessive laryngeal stress, no matter which type of stuttering they reveal.

• 대한후두음성언어의학회지
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• 제8권2호
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• pp.185-192
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• 1997

Behavior of the intrinsic laryngeal muscles : Thyroarytenoid(TA), cricothyroid(CT), lateral cricoarytenoid(LCA), interarytenoid(IA) and posterior cricoarytenoid(PCA) : were evaluated under the in vivo canine laryngeal model in three individual papers. This is the review of the relating three articles. In vivo preparation of the laryngeal model was summarized. Video-laryngoscopic findings of the individual intrinsic laryngeal muscles were documented by electrical stimulation of the individual muscular branches of the laryngeal nerve. Effects on fundamental frequency, subglottic pressure, intensity and open quotient by the stimulation of the individual intrinsic laryngeal muscles were tested.

• Asian Pacific Journal of Cancer Prevention
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• 제14권9호
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• pp.5301-5310
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• 2013

The biological mechanisms of cancer and associations with behavior of tumours need to be studied to understand progression and determine appropriate treatments. Here we investigated expression of VEGF, MMP-9 and E-cadherin in laryngeal SCCs and their relations with clinical behavior. This prospective study was based on 38 surgical specimens from patients with primary laryngeal SCC and data recorded in their cards. Expression of the three factors in tumor tissue was examined using immunohistochemistry and correlations with clinical parameters of primary tumors, regional lymph node metastases, stage of disease, histopathologic differentiation, and vascular/cartilage invasion were investigated. Regarding the cases with positive MMP-9 expression, the difference between well and moderately/poorly differentiated tumors was statistically significant. However, differences between early stage (stage I and II) and late-stage (stage III and IV) tumours, and between positive and negative for pLN metastasis were not. No significant relationship between positive VEGF and tumor differentiation or stage was apparent, but E-cadherin levels significantly differed between well and moderately/poorly differentiated tumours and with the presence of pLN metastasis. E-cadherin staining did not vary between MMP-9 positive and negative cases. In conclusion, MMP-9 may be a negative predictor of differentiation in laryngeal SCC, while E-cadherin is a predictor of differentiation and nodal metastases. Even if the difference between VEGF expression and tumor stage was not statistically significant, it seems that there exists some relationship, which might be clarified with a greater number of cases.

• 대한두경부종양학회지
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• 제6권2호
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• pp.101-107
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• 1990

Most common laryngeal cancer is squamous cell carcinoma, but various kinds of non-squamous cell tumor also occurs in the larynx. It is quite different from epidermoid carcinoma in the aspect of invasive behavior, metastatic route and treatment modality. Its proper diagnosis is somewhat difficult due to its rarity. This paper presents clinical feature of eight non-squamous cell tumor of the larynx experienced during the past 15 years.

• 말소리와 음성과학
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• 제4권1호
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• pp.13-20
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• 2012

A lax stop in Korean, one of the three laryngeal contrastive stops, has undergone a sound change in terms of its acoustic properties. Prior production studies described this recent lax stop as being differentiated from tense and aspirated stops primarily by fundamental frequencies (f0). And, the acoustic property of voice onset time (VOT) further separates tense stops from lax and aspirated stops. The current research explores how these two major acoustic parameters of f0 and VOT cue the three stop categories in Korean adult listeners' perception. Thirty-one native speakers of Korean participated in two experimental tasks: categorization judgment and within-category goodness ratings. Two sets of audio stimuli were prepared by synthesizing English and Korean male speakers' CV productions. The findings showed that while f0 cues listeners to lax stops as production patterns would predict, VOT were closely related to listeners' categorization and goodness ratings of lax stops. This suggests that accurate characterizations of the recent lax stop category need to be based on Korean speakers' perceptual behavior as well as production patterns.

• 대한후두음성언어의학회지
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• 제10권2호
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• pp.124-129
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• 1999

1) 음성 장애를 호소하지 않는 성악인 50명중 여자는 59% 남자는 50%에서 후두스트로보스코피상 성대 병변이 발견되었다. 2) 성종별로는 소프라노에서 가장 성대 병변의 발생률이 높았다(66.7 %). 3) 최장발성 지속시간은 성악인의 경우 성대 병변에 관계없이 일반 정상인에 비해서 증가되어 있었으며 특히 정상 성대를 가진 여자 성악인에서는 일반인에 비해 통계적으로 의의 있게 증가되어 있었다. 4) 기본진동수, 기본진동수 표준편차, 기본진동수변 이들은 성악인이 일반인에 비해 안정되어 있었고, jitter. PPQ는 성악인이 성대 병변이 있더라도 일반인에 비해 감소되어 있었으며, 특히 남자 성악인에서는 통계적으로 유의하게 감소되어 있었고, Shimmer. APQ 역시 성악인이 성대 병변이 있더라도 일반인에 비해 통계적으로 의의 있게 감소되어 있었다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권7호
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• pp.2975-2979
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• 2015

Background: Telomere length is closely associated with cellular radiosensitivity and WRAP53 is required for telomere addition by telomerase. In this research we assessed radiosensitivity of laryngeal squamous cell carcinoma Hep-2 cell lines after WRAP53 inhibition, and analyzed the molecular mechanisms. Materials and Methods: phWRAP53-siRNA and pNeg-siRNA were constructed and transfected into Hep-2 cells with lipofectamine. Expression of WRAP53 was analyzed by RT-PCR and Western-blottin, radiosensitivity of Hep-2 cells was assessed colony formation assay, and the relative length of telomeres was measured by QPCR. Results: The data revealed that the plasmid of phWRAP53-siRNA was constructed successfully, and the mRNA and protein levels of WRAP53 were both obviously reduced in the Hep-2 cell line transfected with phWRAP53-siRNA. After Hep-2 cells were irradiated with X-rays, the $D_0$ and $SF_2$ were 2.481 and 0.472, respectively, in the phWRAP53-siRNA group, much lower than in the control group ($D_0$ and $SF_2$ of 3.213 and 0.592) (P<0.01). The relative telomere length in the phWRAP53-siRNA group was $0.185{\pm}0.01$, much lower than in the untreated group ($0.523{\pm}0.06$) and the control group ($0.435{\pm}0.01$). Conclusions: Decreasing the expression of WRAP53 using RNA interference technique can enhance the radiosensitivity of Hep-2 cell lines by influencing the telomere length. WRAP53 is expected to be a new target to regulate the radiosensitization of tumor cells.

• 대한기관식도과학회:학술대회논문집
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• 대한기관식도과학회 1993년도 제27차 학술대회 초록집
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• pp.85-85
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• 1993

후두 편평세포암은 종양세포의 생물학적 특성과 발생부위별 미세환경이 다르기 때문에 임상적 병기만으로 예후를 추정하는 것은 다소 문제점이 있다. 이에 저자들은 종양세포의 유전정보에 의한 증식능이 예후와 연관성이 있는지 규명하고자 EGFR, p53 단백 및 pRB의 표현양상을 후두 편평세포암 40례의 임상적 특성과 비교 검토하여 다음과 같은 성적을 얻었다. 1. p53 단백의 양성 표현율은 임상적 병기, 원발병소의 병기, 경부 림프전이절의 병기, 병리조직학적 분화도, 재발이나 원격전이 흑은 이차암이 있었던 경우와 연관성이 높았다. 2. pRB의 음성 표현율은 침습성이 강한 성문상분, 임상적 병기, 원발병소의 병기, 병리 조직학적 분화도, 재발이나 원격전이 혹은 이차암, 치료에 실패한 경우와 연관성이 높았다. 3. 3년생존율은 p53의 양성표현율과 관계가 있었다. 4. EGFR은 특별한 상관관계가 없었다. 이상의 결과로 보아 p53 단백과 pRB의 암억제유전자 단백의 면역조직화학적 염색을 통한 표현양상은 후두 편평세포암의 생물학적인 특성을 잘 반영하고 있을 뿐만 아니라 종양 즉 인자들과 밀접한 상관관계가 있어 후두 편평세포암의 악성도를 예측하는데 도움을 주는 예후인자로서 적용시킬 수 있을 것으로 생각된다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2007년도 심포지엄 논문집 정보 및 제어부문
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• pp.475-476
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• 2007

Electroglottography (EGG) is a technique used to register laryngeal behavior indirectly by a measuring the change in electrical impedance across the throat during speaking. However, EGG waveform is affected by laryngeal muscles which fluctuate the vocal cords, and which result in baseline wander. It is required to reduce baseline wander in EGG waveform, because EGG waveform is used for input signal of nonlinear speech synthesizer in next chapter. In vocal cords, the abduction-adduction of glottis is mainly controlled by the posterior cricoarytenoid (abductor) and interarytenoid (adductor) muscles respectively. Empirical Mode Decomposition method was adopted in cancellation of EGG waveform baseline wandering, and showd better performance than that of high pass filter with 500 order.

• 대한후두음성언어의학회지
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• 제8권1호
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• pp.18-26
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• 1997

There are two types of phonetic study, acoustic and physiologic, for differentiating the three manner categories of Korean stop consonants. On the physiologic studies, there are endoscopic, electromyographic(EMG), electroglottographic(EGG) and aerodynamic studies. In this study, I tried to investigate general features of Korean stops using EGG study for the open quotient of vocal fold and baseline shift during speech, and aerodynamic characteristics for e subglottal air pressure, air flow and glottal resistance at consonants. On the aerodynamic study, the glottalized and aspirated stops may be characterized by e increasing subglottal pressure comparing with lenis stop at consonants. The airflow is largest in the aspirated stops followed by lenis stops and glottalized. The glottal airway resistance (GAR) showed highest in the glottalized followed by the lenis, but lowest in e aspirated during e production of consonants, and showed highest in e aspirated, but low in the glottalized and lenis during the production of vowel. The glottal resistance at consonant showed significant difference among consonants and significant interaction between subject and types of consonant. The glottal resistance at vowel showed significant difference among consonants, and e interaction occured between subject and types of consonant. The electroglottography(EGG) has been used for investigating e functioning of e vocal folds during its vibration. The EGG should be related to the patterns of the vocal fold vibration during phonation in characterizing the temporal patterns of each vibratory cycle. The purpose of this study is to investigate the dynamic change of EGG waveforms during continuous speech. The dynamic changes of EGG waveforms fir the three-way distinction of Korean stops were characterized that the aspirated stop appears to be characterized by largest open quotient and smallest glottal contact area of the vocal folds in e initial portion of vocal fold vibration ; the lenis stop by moderate open quotient and glottal contact area ; but the glottalized stop by smallest open quotient and largest glottal contact area. There may be close relationship between the OQ(open quotient) in the initial voice onset and the glottal width at the time of consonant production, the larger glottal width just before vocal fold vibration results in the smaller OQ of the vocal fold vibration in the initial voice onset. The EGG changes of baseline shift during continuous speech production were characterized by the different patterns for the three types of Korean consonants. The small and less stiffness change of baseline shift was found for the lenis and the glottalized, and the largest and stiffest change was found for the aspirated. On the baseline shift for the initial voice onset, they showed so similar patterns with for the consonant production, larger changed in the aspirated. for the lenis and the glottalized during the initial voice onset, three subjects showed individual difference each other. I suggest at s characteristics were strongly related with articulatory activity of vocal tract for the production of consonant, especially for the aspirated stop. The suspecting factors to affect EGG waveforms are glottal width, vertical laryngeal movement and the intrapharyngeal pressure to neighboring tissue during connected spech. So the EGG may be an useful method to describe laryngeal activity to classify pulsing conditions of the larynx during speech production, and EGG research can be controls for monitoring the vocal tract articulation, although above factors to affect EGG would have played such a potentially role on vocal fold vibratory behavior obtained using consonant production.