• International Journal of Industrial Entomology and Biomaterials
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• 제8권1호
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• pp.27-32
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• 2004

With an objective of evolving quantitatively and qualitatively superior bivolitine silkworm hybrid of Bombyxmori L. for tropics with shorter larval duration without compromising on productivity traits, a breeding programme was initiated at this institute during 1997 by utilizing breeding resource material from the institutes Germplasm collection. The breed SD7 is characterized with plain larvae spinning white oval cocoons and SD12 is characterized by sex-limited larval markings spinning white dumb-bell cocoons. After fixation, these breeds along with other newly evolved breeds were subjected for hybrid evaluation in the laboratory. Based on the hybrid studies, the hybrid SD7${\times}$SD12 was selected and evaluated for one year comprising three major sea- sons (6 trials) of tropics viz., Pre-monsoon (characterized by high temperature, low humidity and without any rain fall), Monsoon (characterized by moderate temperature, heavy rain fall with high humidity) and post-monsoon season (characterized by low temperature and low humidity with frequent rain fall) to know the fluctuations in the larval duration. The evaluation studies indicated that the hybrid is having shorter larval duration with productive merits. The hybrid SD7${\times}$SD12 recorded survival percent of 92.74, shell weight of 46.7 cg, shell ratio % of 24.0 and filament length of 1,200 meters with a shorter larval duration of 24 hrs; when compared to productive hybrid CSR2${\times}$CSR5(control). The breeding methodologies and hybrids usefulness for tropics were discussed.

• Molecules and Cells
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• 제25권4호
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• pp.467-478
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• 2008

Simple sequence repeats (SSRs) and interSSR (ISSR) marker systems were used in this study to reveal genetic changes induced by artificial selection for short/long larval duration in the tropical strain Nistari of the silkworm Bombyx mori. Artificial selection separated longer larval duration (LLD) ($29.428{\pm}0.723days$) and shorter larval duration (SLD) ($22.573{\pm}0.839days$) lines from a base, inbred population of Nistari (larval span of $23.143{\pm}0.35days$). SSR polymorphism was observed between the LLD and SLD lines at one microsatellite locus, Bmsat106 ($CA_7$) and at two loci of 1074 bp and 823 bp generated with the ISSR primer UBC873. Each of these loci was present only in the LLD line. The loci segregated in the third generation of selection and were fixed in opposite directions. In the $F_2$ generation of the $LLD{\times}SLD$ lines, the alleles of Bmsat106 and $UBC873_{1074bp}$ segregated in a 1:1 ratio and the loci were present only in the LLD individuals. $UBC873_{823bp}$ was homozygous. Single factor ANOVA showed a significant association between the segregating loci and longer larval duration. Together, the two alleles contributed to an 18% increase in larval duration. The nucleotide sequences of the $UBC873_{1074bp}$ and $UBC873_{823bp}$ loci had 67% A/T content and consisted of direct, reverse, complementary and palindromic repeats. The repeats appeared to be "nested" (59%) in larger repeats or as clustered elements adjacent to other repeats. Of 203 microsatellites identified, dinucleotides (67.8%) predominated and were rich in A/T and T/A motifs. The sequences of the $UBC873_{1074bp}$ and $UBC873_{823bp}$ loci showed similarity (E = 0.0) to contigs located in Scaffold 010774 and Scaffold 000139, respectively, of the B. mori genome. BLASTN analysis of the $UBC873_{1074bp}$ sequence showed significant homology of (nt.) 45-122 with upstream region of three exons from Bombyx. The complete sequence of this locus showed ~49% nucleotide conservation with transposon 412 of Drosophila melanogaster and the Ikirara insertions of Anopheles gambiae. The A + T richness and lack of coding potential of these small loci, and their absence in the SLD line, reflect the active process of genetic change associated with the switch to short larval duration as an adaptation to the tropics.

• International Journal of Industrial Entomology and Biomaterials
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• 제14권1호
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• pp.63-68
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• 2007

The response of silkworm, Bombyx mori L. to phytoecdysteroid (PE) when administered at different ages of $5^{th}$ instar was studied in the popular bivoltine ($CSR2{\times}CSR4$) and multi${\times}$bivoltine ($PM{\times}CSR2$) silkworm hybrids, reared on the Victory-1 variety of mulberry leaves. PE was administered to $5^{th}$ instar silkworm per os at a rate of $250{\mu}g$ per 100 larvae to different batches of silkworm at 0, 24, 48, 72, 96, 120, 144 hrs and at the onset of cocoon spinning when a few larvae were ripe. The larval and mounting duration, cocoon yield and cocoon characters were influenced by PE. The intensity of influence was dependent on the time of application. The larvae treated at the beginning of the instar, improved the economic traits significantly with a marginal increase in larval duration. In the larvae treated at the middle of the instar, larval duration was shortened remarkably but the economic traits were adversely affected. This particular treatment can become a good management strategy in the case of mulberry leaf shortage or disease incidence. In the larvae treated at the onset of cocoon spinning, the mounting duration was substantially reduced without much effect on the cocoon traits which would be a big benefit in commercial sericulture. The physiological significance of varied response of silkworm to PE administration is discussed.

• 한국잠사곤충학회지
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• 제34권2호
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• pp.13-19
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• 1992

몇 가지 교잡원종에 있어서 란의 크기가 차대잠의 계량형질에 미치는 영향에 대한 실험결과는 다음과 같다. 1. 공시한 모든 품종에 있어서 란의 크기와 의잠체중은 정의 상관관계가 있었으며 2령까지의 유충체중에 있어서도 대형란구의 유충이 가장 무거웠고 소형란구의 유충이 가장 가벼웠다. 장잠기 유충체중은 유럽종 E16을 제외한 모든 품종에서 대형란구의 유충이 가장 무거웠으나 정상란구와의 차이는 적었다. 2. 유충경과일수에 있어서는 유럽종 El6을 제외하고는 대형란구가 가장 짧았으며 란의 크기와 화용비율과는 일정한 경향이 없었다. 유충체중, 유충경과일수 등의 형질은 품종에 따른 영향이 란의 크기에서 오는 영향보다 컸으며 견형질에 있어서는 대형란구가 가장 우수한 것으로 나타났다.

• International Journal of Industrial Entomology and Biomaterials
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• 제40권2호
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• pp.41-50
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• 2020

Vermicompost, manure, compost and organic fertilization are ecofriendly. Nowadays, many products resulted from sericulture consumed by humans such as mulberry leaves, fruits, mulberry tea, silk and natural silk cosmetics. Soil applications of three treatments with vermicompost (0.5, 1 and 2 tons per 0.42 hectare) and recommended rate of mineral fertilizers of nitrogen phosphorus potassium were used for investigation. Impact of fertilization on mulberry plant traits of moisture, number of shoots/tree, total shoots length/tree, number of leaves/shoot, number of leaves/ (100g), leaf yield/tree and leaf yield of fadden/season were recorded. In addition the effect of fertilization on larval and cocoon characters of young instar duration, fifth instar duration, total larval duration, larval mortality percentage, weight of third instar larvae, weight of fourth instar larvae, weight of fifth instar larvae, fresh cocoon weight, fresh shell weight, pupae weight, cocoon shell ratio, silk productivity, cocooning percentage, pupation ratio, number of cocoons/ liter, crop cocoons by number, crop cocoons by weight, fecundity and fertility. Using vermicompost treatment was enhancing plant characters. Treatments of V₃, V₂ and V₁ were shortage young, fifth and larvae durations. Mostly feeding silkworm during the whole larval duration on treated mulberry leaves with vermicompost improving the traits average. Using vermicompost for fertilization by rate of V₃ and V₂ is better than others for cocoon characters for females and males.V₃ and V₂ of vermicompost per 0.42 hectare is recommended for rearing mulberry silkworm instead of mineral fertilization.

• 한국수산과학회지
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• 제45권6호
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• pp.716-722
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• 2012

The larval occurrence of the red snow crab Chionoecetes japonicus was investigated near Dokdo in the East Sea of Korea, and the larval timing of the genus Chionoecetes in world oceans was reviewed. C. japonicus larvae were collected seasonally at 12 stations in February, May, August, and November in 2011. A Bongo net with a 303 mesh was deployed with a double oblique tow. Larvae appeared from February to August, but no larvae were found in November. Zoea I occurred at nine stations with a few zoea II in February, and were found until August. The larval densities in February were high near the northern. In May, megalopa were found at eight stations with a few zoea II. No larvae were caught in November. The larval abundance in the research area peaked in February. Overall, the larval duration of the genus Chionoecetes in the world's oceans persisted for a long time, and Chionoecetes larvae occurred during the warm season in each area. The cold temperature at the sea bottom, where gravid females are found, may prolong larval production along the distribution range of the genus Chionoecetes.

• Ocean Science Journal
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• 제40권3호
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• pp.177-182
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• 2005

The process of embryogenesis and larval development of the asteroid sea star Asterias amurensis $(U{\ddot{u}}tken)$ was observed, with special attention paid to morphological change and larval duration. In reproductive season, mature sea stars were collected under floating net cages, located in Tongyeong, southern Korea. The mature eggs are $138\;{\mu}m$ in average diameter, semi-translucent and orange in color, sperms in good condition appear light cream to white-gray in color. Embryos develop through the holoblastic equal cleavage stage and a wrinkled blastula stage that lasts about 9 hours after fertilization. Gastrulae bearing an expanded archenteron hatch from the fertilization envelope 22 hours after fertilization. At the end of gastrulation, rudiments of the left and right coelom are formed. By day 2, larvae possess complete alimentary canal and begin to feed. At this stage, the larva is called early bipinnaria. In 6-day-old larvae, the pre- and post- oral ciliated bands form complete circuits and the bipinnarial processes start to develop. By day 12, the lateral and anterior projection of the larval wall processes along the ciliated bands begins to thicken and curl, and the ciliated bands become more prominent. By day 32, early brachiolaria are presented with three pairs of brachiolar arms. Advanced brachiolaria with a well-developed brachiolar complex (three pairs of brachia and central adhesive disc) occur 6 weeks after fertilization. In the field, spawning of the sea star was observed in April to May, settlement form larvae and just settlements seem to occur from June to July, and early juveniles occur from August to September. Although we had not described the end of brachiolaria stage, it can be tentatively estimated that the duration of the pelagic stage of A. amurensis is 40 to 50 days.

• 한국잠사곤충학회지
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• 제27권1호
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• pp.18-23
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• 1985

인공사료육 누에에 있어서 사료조성 차이와 사육환경이 누에의 성장발육에 미치는 영향은 다음과 같다. 1. 사료의 대두분말 함량이 많을수록 경과가 짧았으나 대두분말 함량이 50%를 넘으면 오히려 경과가 길어졌다. 2. 감잠비율은 대두분말 함량비가 50%까지는 영향을 미치지 못하지만 그 이상에서는 높았다. 3. 사료조성 차이가 견질에 미치는 영향은 단견중에 있어서는 단백질 함량이 많을수록 무겁고 견층중은 차이가 없었으며 견층비율은 대조구에 비해 각 처리구 모두 낮았다. 4. 사육환경 중 온도는 고온이 광선은 암조건이 경과를 단축시켰다. 5. 감잠비율은 치잠기 고온에서 낮았고 광선은 암조건에서 낮았다. 6. 사육환경이 견질에 미치는 영향은 단견중, 견층중, 견층비율 모두 온도는 고온이, 광선은 암조건이 양호하였다.

• 한국잠사곤충학회지
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• 제32권1호
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• pp.38-43
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• 1990

Effects of topical application of 100, 200 and 300ng/$m\ell$ of 3-indole butyric acid (3IBA) on larval parameters were studied in polyvoltine Pure Mysore breed of B. mori. Each concentration was administered independently, at 36h to III, IV ＆ V instars, at 48h to IV ＆ V instars, and at 72h to V instar larvae. Of the various dosages used, the repeated applications of loons of 3-IBA at 36h to III, IV ＆ V instars resulted in a significant increase in larval weight, silk gland weight, cocoon shell weight and fecundity and a significant decrease in larval duration and cocooning percentage when compared with the corresponding parameters of the untreated controls.

• International Journal of Industrial Entomology and Biomaterials
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• 제32권2호
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• pp.69-79
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• 2016

Distillers dried grain (DDG) and makgeolli spent grain (MSG) are agricultural byproducts to produce alcoholic beverage. However, they are known to contain enough nutrients. Mealworm is a promising insect resource for an animal feed ingredient as well as alternative human food. With low cost, DDG and MSG were investigated as a feed ingredient for rearing high quality mealworms. DDG and MSG were mixed with wheat bran and compared to control feed (only wheat bran) for its effects on larval survivorship, larval weight, duration for developmental period, pupation rate, and pupal weight. When DDG added, larval survivorship was reduced to 50~70% compared to the control group. Larvae fed on DDG were heavier from third to sixth week. Especially, larvae with 50% DDG were 28% heavier than the control group at the third week. For the larval period, the 50% DDG group was 11% less than that for the control. The pupal weight for the 30% DDG group was 7% heavier than that for the control group. Pupation rates for all the DDG groups were higher than 90%. When compared to the control, larval survivorship for the 70% MSG group was low, but the 50% and 70% MSG groups were high during the seventh and eighth weeks because of delayed development. After the eighth week, larvae with 70% MSG showed the highest larval weight increase as 9~18% compared to the control group. Except 70% MSG group, all of MSG groups showed more than 90% pupation rates. We confirmed that adding 30~50% of DDG or MSG to conventional wheat bran have a strong potential to replace the conventional wheat bran insect feed for quality insect production.