• 제목/요약/키워드: larval development

검색결과 555건 처리시간 0.023초

꼬막 (Tegiilarca granosa) (Linngeus)의 산란유발 및 난 발생과 초기 유생 사육 (Spawning Inducement, Egg Development and Early Larval Rearing of Ark Shell (Tegillarca granosa) (L.))

  • 문태석;정민민;신윤경;양문호;고창순;장영진
    • 한국수산과학회지
    • /
    • 제37권6호
    • /
    • pp.485-491
    • /
    • 2004
  • Spawning induction, egg development and larval growth of ark shell (Tegillarca granosa) (L.) were investigated. The most effective method of spawning induction was steady temperature increasing from$4^{\circ}C\;to\;28^{\circ}C$ with irradiation of sea water by UV after T. granosa was exposed to air at $4^{\circ}C$ Optimum condition for larval roaring was under the 32.4 psu and two temperature $regimes:\;28{\pm}1^{\circ}C\;and \;25{\pm}1^{\circ}C$. Fertilized eggs was demersal isolated eggs, and egg diameter was $60{\mu}$. D-shaped larvae appear about 20 hr after hatching with $94.1{\mu}$ in shell length and $86.7{\mu}$ in shell height. Ten days were required from hatching to umbo larva stage, of a mean shell length $125.2{\mu}$. On 25th day, the larva grew to $450{\mu}$ in shell length and began to settle on the bottom. Effect of temperature between $25^{\circ}C$ (control group) and $28^{\circ}C$ on larval growth was not different. Survival rate of larvae settled on the bottom was about $19{\%}$ in both temperatures conditions $(25^{\circ}C\;and\;28^{\circ}C)$.

LIN-23, an E3 Ubiquitin Ligase Component, Is Required for the Repression of CDC-25.2 Activity during Intestinal Development in Caenorhabditis elegans

  • Son, Miseol;Kawasaki, Ichiro;Oh, Bong-Kyeong;Shim, Yhong-Hee
    • Molecules and Cells
    • /
    • 제39권11호
    • /
    • pp.834-840
    • /
    • 2016
  • Caenorhabditis elegans (C. elegans) utilizes two different cell-cycle modes, binucleations during the L1 larval stage and endoreduplications at four larval moltings, for its postembryonic intestinal development. Previous genetic studies indicated that CDC-25.2 is specifically required for binucleations at the L1 larval stage and is repressed before endoreduplications. Furthermore, LIN-23, the C. elegans ${\beta}$-TrCP ortholog, appears to function as a repressor of CDC-25.2 to prevent excess intestinal divisions. We previously reported that intestinal hyperplasia in lin-23(e1883) mutants was effectively suppressed by the RNAi depletion of cdc-25.2. Nevertheless, LIN-23 targeting CDC-25.2 for ubiquitination as a component of E3 ubiquitin ligase has not yet been tested. In this study, LIN-23 is shown to be the major E3 ubiquitin ligase component, recognizing CDC-25.2 to repress their activities for proper transition of cell-cycle modes during the C. elegans postembryonic intestinal development. In addition, for the first time that LIN-23 physically interacts with both CDC-25.1 and CDC-25.2 and facilitates ubiquitination for timely regulation of their activities during the intestinal development.

Breeding of a Silkworm Variety for Synnemata Production of Isaria tenuipes

  • Kang, Pil-Don;Sung, Gyoo-Byung;Kim, Kee-Young;Kim, Mi-Ja;Hong, In-Pyo;Ha, Nam-Gyu
    • Mycobiology
    • /
    • 제38권3호
    • /
    • pp.180-183
    • /
    • 2010
  • This study was conducted out to select a silkworm variety suitable for synnemata production of Isaria tenuipes. Four kinds of the mulberry silkworm varieties, Bombyx mori, were hybridized using a Japanese parental line and a Chinese parental line, and used to test for synemata formation in I. tenuipes. The larval period of normal silkworms was 22 hr longer than the silkworms inoculated with this fungus. Among the silkworm varieties tested, Hachojam had the shortest larval period with 23.02 days. The non-cocooning silkworm had a shorter larval period than the cocoon producing silkworms. The pupation rate of normal silkworms was about 9% higher than that of silkworms sprayed with I. tenuipes. Hachojam had the highest infection rate at 99.8%, but no significant difference was observed for the infection rate by silkworm variety. The production of synnemata was the best in JS171 $\times$ CS188 with an incidence rate of 99.3%, followed by Hachojam, and Chugangjam. The synnemata produced from Hachojam were the heaviest and showed white or milky-white in color.

등줄숭어, Chelon affinis(Gunther)의 난발생 및 자어의 형태발달 (Egg and Larval Development of Chelon affinis by Artificial Treatment in Aquarium)

  • 김용억;김진구
    • 한국수산과학회지
    • /
    • 제32권2호
    • /
    • pp.134-138
    • /
    • 1999
  • 1997년 3월에 부산시 민락동 어시장에서 채집한 등줄숭어 어미들을 실험실로 운반하여 인위적인 방법으로 난을 수정시킨 후, 난의 형태, 난발생과정 및 자어의 성장에 따른 형태변화를 관찰하였다. 1. 등줄숭어의 수정란은 무색투명한 원형의 분리부성란으로, 난경은 0.95$\~$1.06mm이고 부화에 소요된 시간은 수정후 $16.0^{\circ}C$에서 73시 간이였다. 2. 부화 직후의 자어는 전장 1.72$\~$1.92mm로 입과 항문은 열리지 않고, 근절은 19개로 아직 정수에 달하지 않았으며, 몸에는 막대기 모양의 흑색소포가 산재한다. 3. 부화후 5일째의 자어는 전장 2.27$\~$2.49mm로 입이 최초로 열리며 동시에 난황이 완전히 흡수되어 후기자어로 이행한다.

  • PDF

낙동강 하구역에 출현하는 청어(Clupea pallasii) 자치어의 식성 (Feeding Habits of Larval Clupea pallasii from the Nakdong River Estuary, Korea)

  • 최희찬;박주면;윤석현;허성회
    • 한국수산과학회지
    • /
    • 제48권4호
    • /
    • pp.498-506
    • /
    • 2015
  • We examined the feeding habits of larval Clupea pallasii using 148 specimens collected from December 2010 to April 2011 in the Nakdong River estuary, Korea. We found that larval C. pallasii [8.3-26.0 mm NL (notochord length)] are carnivorous, consuming mainly copepods, rotifers, cirripeds, and free-living flatworms (Macrostomida). Their diet also include small quantities of shrimp nauplii, tintinnids, cladocerans, and larval bivalves, among other food sources. To evaluate ontogenetic changes in dietary composition, we split larvae into four size groups: 8-12 mm, 12-16 mm, 16-20 mm, and > 20 mm NL. Rotifers were the most important prey items for the smallest group, but all other groups predominantly consumed copepods. The largest group frequently preyed on rotifers, cirripeds and flatworms, in addition to copepods. Larvae also showed bi-modal feeding, with peaks in the late afternoon and at midnight, wherein the mean number of prey per individual peaked at 1,800-1,900 h.

농게(갑각강, 게아목, 달랑게과) 의 유생발생 (Complete Larval Development of Uca Arcuata(Crustacea, Brachyura, Ocypodidae) Reared in the Laboratory)

  • 고현숙;김창현
    • Animal Systematics, Evolution and Diversity
    • /
    • 제5권2호
    • /
    • pp.89-105
    • /
    • 1989
  • 농게(Ura arcuata) (달랑게과, 달랑게아과) 의 유생을 부화에서부터 첫 번째 게기까지 수온 $25^{\circ}C$ , 염분농도 33.3$\textperthousand$의 해수에서 사육하고, 각 유생기이 형태적인 특징을 기술 및 도식하였다. 이 종은 5기의 zoea와 1기의 megalopa 유생을 거쳐 첫 번째 게기로 변태하였다. 제 1 zoea 유생기는 제 1 소악의 내지에 4개의 말단강모와 제 2 악각의 기절과 내지에 각각 3개의 우상강모와 0, 0, 5 강모식을 가지고 촉각은 B형이었다. 이러한 특징들은 이미 보고된 같은 과의 유생들의 특징들과 잘 일치하고 있다.

  • PDF

실험실에서 사육된 털콩게 Ilyoplax dentimerosa Shen, 1932의 유생발생 (Larval Development of llyoplu dentimerosa Shen, 1932 (Decapoda, Brachyura, Ocvpodidae) Reared in the Laboratory)

  • 고현숙;김창현
    • 한국동물학회지
    • /
    • 제34권1호
    • /
    • pp.93-102
    • /
    • 1991
  • Larvae of Ilyoplax dentimerosa Shen, 1932 were reared in the laboratory at $25^{\circ}C$ and 33.3%o salinity. Four zoeal stages were recognized in the lanral development, and these are described and illustrated. The first soeae of 1. dentimerosa are distinguished from other llyoplax zoeae by having minute spicules on the rostral and dorsal carapace spines, a short dorsal carapace spine about half of the carapace length, a conspicuous antennal exopodite with a subterminal sutta and acute spines on the postero-lateral border of the fourth abdominal somite.

  • PDF

Larval Development of Latreutes acicularis Ortmann (Crustacea: Decapoda: Hippolytidae) Reared in the Laboratory

  • Yang, Hoi-Jeong
    • Animal cells and systems
    • /
    • 제11권1호
    • /
    • pp.79-92
    • /
    • 2007
  • Eight zoeal stages and one decapodid of the hippolytid shrimp Latreutes acicularis Ortmann, 1890 are described from laboratory-reared material hatched from egg from ovigerous females collected from Shimoda, Japan. The presence of a minute dorsomedian protuberance on the third abdominal somite, the spinules on the dorsal margin of the fourth and fifth abdominal somites, and a pair of dorsolateral spines on the fourth and fifth abdominal somites readily distinguish the larvae of L. acicularis from those of L. anoplonyx Kemp, 1914 and L. laminirostris Ortmann, 1890, the other two known larvae of Latreutes from East Asia.

동남참게 Enocheir japonicus의 유생 발생 (The Complete Larval Development of Enocheir japonicus De Haan (Crustacea, Brachytira, Grapsidae) Reared in the Laboratory)

  • 김창현;황상구
    • 한국동물학회지
    • /
    • 제33권4호
    • /
    • pp.411-427
    • /
    • 1990
  • 실험실에서 사육된 동남참게(참게아과) 전체 유생기의 형태적인 특징을 상세히 기술하고 도시하였다. 동남참게는 5 zoea기를 거쳐 megalopa기로 탈피하였다. 수온 $25^{\circ}C$, 염분 농노 33.3$\textperthousand$, 광주기 14 h light:10 h dark의 조건하에서 실험이 진행되었다. 부화로부터 megalopa기와 제1 carb기 까지는 각각 17일과 27일이 걸렸다. 각 유생기이 형태적인 특징들이 이미 기재된 일본산 동남참게의 유행 특징들과 비교되었으며, 참게아과 내의 Zoea I기의 형태적 특징에 관한여 간단히 논의하였다.

  • PDF

Effect of water temperature on embryonic development and larval survival of an intertidal snail, Nassarius festivus (Powys, 1835)

  • Kang, Sin-Kil;Sung, Chan-Gyoung;Chung, Jiwoong;Park, Dong-Ho;Lee, Jong-Hyeon;Lee, Chang-Hoon
    • 한국패류학회지
    • /
    • 제32권1호
    • /
    • pp.37-43
    • /
    • 2016
  • This study was carried out to determine the optimal water temperature for the embryonic development and laboratory culture of larvae of an intertidal mud snail, Nassarius festivus. The embryos and hatched veliger larvae of N. festivus were incubated at six different temperatures (5, 10, 15, 20, 25 and $30^{\circ}C$). Developmental time for each stage decreased as water temperature increased. The elapsed time to develop to the veliger larva at 15, 20, 25 and $30^{\circ}C$ was 559, 155, 131 and 103 hrs, respectively. At 5 and $10^{\circ}C$, embryo developed to veliger larvae but failed to hatch out of the egg capsule. In contrast, all embryos successfully hatched in the temperature range from 15 to $30^{\circ}C$. The biological minimum temperature during the embryonic development of N. festivus was estimated to be $9.5{\pm}0.4^{\circ}C$. The cumulative water temperatures for blastula, gastrula and veliger stages were calculated as $111{\pm}84$, $486{\pm}185$, $1,164{\pm}72^{\circ}C$, respectively. Temperature also affected the larval survival. Five days after hatching, more than 84% of larvae survived at all experimental temperatures. However, survival began to decrease after 6 days. It was 0% at $30^{\circ}C$. Survival of larvae incubated for 8 days was higher at 15 and $20^{\circ}C$ than other experimental temperatures. We therefore suggest that the optimal range of temperature for embryonic development and larval survival of N. festivus is $15-20^{\circ}C$.