• Proceedings of the Korean Biophysical Society Conference
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• 2001.06a
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• pp.38-38
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• 2001

We cloned the cDNA encoding the neuron-specific $\beta$-subunit ($\beta$4) of large-conductance calcium-activated potassium channels from rat brain and determined the DNA sequences of the entire coding region (GenBank accession; AY028605). The deduced amino acid sequences of r$\beta$4, 210 amino acids in length, are closely related to the $BK_{Ca}$ $\beta$4 subunits of other species but show only limited sequence homology to other $\beta$-subunits, $\beta$1-$\beta$3.(omitted)d)

• The Korean Journal of Mycology
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• v.47 no.1
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• pp.35-42
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• 2019

We isolated endophytic fungi from the conifer leaves of Pinus densiflora inhabiting Buan-gun, Jeollabuk-do, Korea. We identified the isolated fungal strains based on phylogenetic analysis performed using the nucleotide sequences of the internal transcribed spacer, large subunit, and beta-tubulin. We confirmed the presence of three novel endophytic fungi in Korea, namely Paracamarosporium hawaiiense, Tubakia dryina, and Zasmidium fructigenum. In this report, we described the morphological characteristics of these fungal strains and the results of their phylogenetic analysis.

• The Korean Journal of Mycology
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• v.50 no.3
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• pp.195-204
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• 2022

A fungal strain, designated KNUF-20-NI009, was isolated from soil collected from Gunsan-si, Jeollabuk-do, Korea. The isolate showed cultural features typical of the genus Xenoroussoella. Colonies cultivated on malt extract agar were olivaceous-brown to pale olivaceous-white at the margins, with undersides of dark olivaceous to olivaceous-brown and a white margin. The conidia, with a size range of 2.7-5.1×1.6-3.3 ㎛ ($\bar{x}=3.6\times2.6{\mu}m$, n=50), were globoid to ellipsoid in shape, hyaline when immature, becoming light brown to golden-brown when mature, and characterized by 1 or 2 guttules. Multi-locus sequence analysis based on a combined dataset of internal transcribed spacer regions (ITS), large subunit rDNA (LSU), small subunit rDNA (SSU), translation elongation factor 1-alpha (TEF1α), and RNA polymerase II largest subunit (RPB2) sequences revealed KNUF-20-NI009 to be a strain of Xenoroussoella triseptata. This is the first report of this species in Korea.

• The Korean Journal of Mycology
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• v.47 no.4
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• pp.313-318
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• 2019

In the present study, we isolated endophytic fungal strains from the rhizoids of the moss Funaria hygrometrica. The isolated strains were identified based on morphological characteristics and analysis of the internal transcribed spacer (ITS) and large subunit (LSU) rDNA sequence regions. Consequently, we confirmed the presence of two endophytic fungal species, Curvularia protuberata and Didymella anserina, which have not been reported in Korea previously. Here, we describe the morphological characteristics and molecular analysis results of these fungal species.

• Journal of Life Science
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• v.11 no.2
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• pp.133-137
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• 2001

The molecular taxonomic relationship of nine species in the genus Moniliella Stolk & Dakin and Trichosporonoides Haskins & Spencer and six species of other yea나-like fungi was examined by sequencing analysis of large subunit rDNA D1/D2 variable domain. The fifteeen species fell into two major groups corresponding with their genetic relationships. The nine species of the genus Moniliella and Trichosporonoides were placed at the same cluster. similarity values based on the D1/D2 domain sequences were 45.4-100% among species of genus Moniliella, 45.2-84.4% among genus Trichosporonoides species, and 45.6-90.1% among species of genus Moniliella and Trichosporonoides. Identical sequence similarity was observed between M. suaveolens var. nigra and M. suaveolens. A colse relationship of M. mellis. and M. acetoabutens is observed. The result of this study provided and insight into the genetic origins of genus Moniliella and Trichosporonoides species as well as their genetic relationships. Genus Moniliella and Trichosporonoides are closely related to each other based on sequence analysis of the large subunit rDNA D1/D2 region and we suggest combination of the genus Moniliella and Trichosporonoides to single genus.

• The Korean Journal of Mycology
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• v.47 no.2
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• pp.113-120
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• 2019

We isolated endophytic fungal strains from the roots of three medicinal crops, Ligusticum chuanxiong, Angelica gigas, and Cnidium officinale, cultivated at Yeongju, Korea. The fungal strains were identified based on their morphological characteristics and molecular analyses of their internal transcribed spacer, large subunit rDNA, and beta-tubulin regions. Thereby, we identified three previously unrecorded endophytic fungal species, Dactylonectria pauciseptata, Rhizopycnis vagum, and Sistotrema sernanderi, in Korea. In this report, we describe the morphological characteristics and phylogenetic analyses of these three fungal species.

• Research in Plant Disease
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• v.28 no.4
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• pp.248-251
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• 2022

In October 2022, common Asian cowpea (Vigna nakashimae) powdery mildew disease was investigated at Yesan-gun, Chungcheongnam-do, Korea (36°40'12.7"N,126°51'36.4"E). The disease mainly occurred on the front of the leaves, and weak powdery mildew disease occurred on the back side of leaf. The chasmothecia was identified on the back side of the leaf. Analysis of the internal transcribed spacer rDNA sequence and 28S large subunit sequence, along with the morphological characteristics of the anamorph and teleomorph of the causal fungus identified as Podosphaera xanthii. This is the first time in Korea to report common Asian cowpea (Vigna nakashimae) powdery mildew disease caused by Podospaera xanthii.

• Journal of the korean society of oceanography
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• v.39 no.3
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• pp.163-171
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• 2004

Sequences of the large subunit ribosomal (LSD) rDNA D1-D2 region of Alexandrium catenella(=A. sp. cf. catenella) and A. tamarense isolates, which were collected along the Korea coasts, were analyzed to understand their phylogenetic relationships and geographical distributions. All A. catenella and A. tamarense isolates belonged to the A. tamarense/catenella/fundyense complex and were grouped with the North American and temperate Asian ribotypes, respectively, regardless of the presence or absence of a ventral pore in the first apical plate. A consistent and peculiar characteristic that differentiated the Alexandrium isolates was amplification of a second PCR product with a lower molecular weight in addition to the predicted one; ten A. catenella isolates belonging to the temperate Asian ribotype yielded this additional PCR product. Sequence alignment revealed that the shorter PCR product resulted from an unusual large deletion of 87 bp in the LSD rDNA D1 domain. The North American and temperate Asian ribotypes were prevalent along the Korean coasts without geographical separation. Given the high genetic homogeneity among widely distributed Alexandrium populations, each ribotype appeared to be pandemic rather than to constitute a distinct regional population.

• The Korean Journal of Mycology
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• v.51 no.3
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• pp.155-165
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• 2023

In this study, we isolated several fungal strains from filtered water and sediment collected from rivers and streams. The strains were identified by molecular phylogenetic analyses of rDNA sequences (internal transcribed spacer [ITS], large subunit of ribosomal DNA [LSU]). The morphological characteristics of the fungi were investigated using microscopy, and the culture characteristics of fungi grown on several media were examined. We identified four species previously unknown in South Korea, namely, Dissophora globulifera, Linnemannia exigua, Mortierella rishikesha and Umbelopsis autotrophica.

• ALGAE
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• v.28 no.4
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• pp.307-330
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• 2013

The genus Cryptomonas is easily recognized by having two flagella, green brownish color, and a swaying behavior. They have relatively simple morphology, and limited diagnostic characters, which present a major difficulty in differentiating between species of the genus. To understand species delineation and phylogenetic relationships among Cryptomonas species, the nuclear-encoded internal transcribed spacer 2 (ITS2), partial large subunit (LSU) and small subunit ribosomal DNA (rDNA), and chloroplast-encoded psbA and LSU rDNA sequences were determined and used for phylogenetic analyses, using Bayesian and maximum likelihood methods. In addition, nuclear-encoded ITS2 sequences were predicted to secondary structures, and were used to determine nine species and four unidentified species from 47 strains. Sequences of helix I, II, and IIIb in ITS2 secondary structure were very useful for the identification of Cryptomonas species. However, the helix IV was the most variable region across species in alignment. The phylogenetic tree showed that fourteen species were monophyletic. However, some strains of C. obovata had chloroplasts with pyrenoid while others were without pyrenoid, which used as a key character in few species. Therefore, classification systems depending solely on morphological characters are inadequate, and require the use of molecular data.