• 한국균학회지
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• 제45권2호
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• pp.102-106
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• 2017

A fungal strain was isolated from surface-sterilized roots of Rhododendron mucronulatum, a plant species belonging to the Ericaceae family, collected from Mt. Minjujisan, Korea. This fungal strain was identified as Rhizodermea veluwensis based on its morphological characteristics and based on phylogenetic analysis of its internal transcribed spacer regions and large-subunit rDNA. R. veluwensis has not been previously reported in Korea, and for the first time, we report and describe it herein.

• 한국균학회지
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• 제46권4호
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• pp.369-374
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• 2018

The fungal strain KNU16-004 was isolated from a field soil sample collected in Seoul. The isolate was identified as Leptosphaerulina australis based on morphological characterization and phylogenetic analysis using the internal transcribed spacer (ITS), large subunit (LSU) rDNA regions, and ${\beta}-tubulin$ (Tub2). This is the first report of Leptosphaerulina australis in Korea.

• 한국균학회지
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• 제51권1호
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• pp.1-6
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• 2023

Tilia mandshurica trees with rust symptoms have consistently been noticed during disease surveys in Korea since 2006. Based on the morphological examination and molecular sequence analysis of the internal transcribed spacer and large subunit of ribosomal DNA, Pucciniastrum tiliae was identified as the causal fungus of rust disease. This is the first report of P. tiliae infection on T. mandshurica in Korea.

• 한국균학회지
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• 제51권4호
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• pp.307-311
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• 2023

Powdery mildew anamorphs were collected from Orixa japonica (Rutaceae) in Korea. Based on the morphology and molecular phylogeny derived from the internal transcribed spacer regions and the large subunit gene of the rDNA, the fungus was identified as Erysiphe orixae. This powdery mildew species has been known to be endemic to Japan. This is the first report on E. orixae in Korea.

• 한국균학회지
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• 제51권3호
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• pp.155-165
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• 2023

In this study, we isolated several fungal strains from filtered water and sediment collected from rivers and streams. The strains were identified by molecular phylogenetic analyses of rDNA sequences (internal transcribed spacer [ITS], large subunit of ribosomal DNA [LSU]). The morphological characteristics of the fungi were investigated using microscopy, and the culture characteristics of fungi grown on several media were examined. We identified four species previously unknown in South Korea, namely, Dissophora globulifera, Linnemannia exigua, Mortierella rishikesha and Umbelopsis autotrophica.

• 한국균학회지
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• 제52권1호
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• pp.13-18
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• 2024

We isolated endophytic fungal strains from twigs of Larix gmelinii var. olgensis. These strains were identified based on their morphological characteristics and phylogenetic analyses including internal transcribed spacers and large subunit rDNA. We confirmed the presence of a previously unrecorded fungal species in Korea, Aequabiliella effusa. This study presents the morphological characteristics and phylogenetic analysis of A. effusa.

• BMB Reports
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• 제35권2호
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• pp.194-198
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• 2002

Eukaryotic replication protein A (RPA) is a single-stranded(ss) DNA binding protein with multiple functions in DNA replication, repair, and genetic recombination. The 70-kDa subunit of eukaryotic RPA contains a conserved four cysteine-type zinc-finger motif that has been implicated in the regulation of DNA replication and repair. Recently, we described a novel function for the zinc-finger motif in the regulation of human RPA's ssDNA binding activity through reduction-oxidation (redox). Here, we show that yeast RPA's ssDNA binding activity is regulated by redox potential through its RPA32 and/or RPA14 subunits. Yeast RPA requires a reducing agent, such as dithiothreitol (DTT), for its ssDNA binding activity. Also, under non-reducing conditions, its DNA binding activity decreases 20 fold. In contrast, the RPA 70 subunit does not require DTT for its DNA binding activity and is not affected by the redox condition. These results suggest that all three subunits are required for the regulation of RPA's DNA binding activity through redox potential.

• BMB Reports
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• 제42권2호
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• pp.101-105
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• 2009

The homozygous T-DNA mutant of the AMY1 gene in Arabidopsis was identified and importantly, shown to cause an early flowering phenotype. We found that the disruption of AMY1 enhanced expression of CO and FT. The expression analyses of genes related to starch metabolism revealed that expression of the AGPase small subunit APS1 in the wild type was higher than in the amy1 mutant. However, there were no significant differences in expression levels of the AGPase large subunit genes ApL1, AMY2, or AMY3 between wild type and the amy1 mutant. Expression profiling showed that AMY1 was highly expressed in leaves, stems, and flowers, and expressed less in leafstalks and roots. Furthermore, the level of AMY1 mRNA was highly elevated with age and in senescing leaves. RT-PCR analyses showed that the expression of AMY1 was induced by heat shock, GA, and ABA, while salt stress had no apparent effect on its expression.

• Applied Microscopy
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• 제30권4호
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• pp.413-421
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• 2000

초고온 archaeon인 Thermococcus profundus에서 실린드형태의 단백질 복합체를 분리, 생화학적 특성과 구조를 규명하였다. 전자현미경과 영상처리 (image processing)를 이용한 구조 분석으로 8개의 subunit으로 이루어진 링 (ring) 형태가 두 개 겹쳐져 가운데 통로를 가지는 실린드형태로 이루어진 복합체임을 알 수 있었다. 또한 60kDa단백질(P60 complex)로 구성된 homomultimer임을 보여 주고 있다. 이 복합체는 $80^{\circ}C$ 이상에서 도 강한 ATPase의 활성을 보여주는 강한 내열성 단백질임을 알 수 있다. P60 complex는 초고온 archaeon이 높은 온도에서 적응하며 생존하는데 매우 중요한 역할을 할 것으로 추정할 수 있다.

• ALGAE
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• 제32권2호
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• pp.87-100
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• 2017

The genera Hypnea and Calliblepharis of the family Cystocloniaceae are discriminated by their female reproductive structure, especially in the formation of carposporangia and gonimoblasts. Hypnea saidana, once classified based on obsolete evidence, has not been studied phylogenetically using molecular analysis and detailed reproductive structure though it shares many morphologic features with the genus Calliblepharis. To provide better understanding of generic relationship of H. saidana with Hypnea and Calliblepharis, we carried out molecular analyses using the nuclear-encoded small subunit ribosomal DNA (SSU) and chloroplast-encoded large subunit of the RuBisCO (rbcL), and exact morphological observations focusing on the reproductive structures of wild specimens. Our molecular phylogeny showed that H. saidana is closely related to Calliblepharis, but distinct from the clade of Hypnea. Female reproductive structure of H. saidana characterized by upwardly developing chains of carposporangia, central reticulum of cell, and gonimoblast filaments not connected to the pericarp provides definite evidence to assign the taxonomic position of this species to Calliblepharis. Based on our combined molecular and morphological analyses, we have proposed Calliblepharis saidana comb. nov., expanding the distribution of Calliblepharis habitat from the eastern Atlantic South Africa, the northern Indian Ocean, Australasia, and Brazil to the western Pacific Ocean.