• 제목/요약/키워드: land snail

검색결과 41건 처리시간 0.031초

동양달팽이 Nesiohelix samarangae 소화관에서의 cellulase 활성에 대한 세포화학적 및 면역세포화학적 연구 (Cytochemical and Immunocytochemical Study on the Cellulase Activity in the Digestive Tract of the Land Snail Nesiohelix)

  • 정계헌;이용석;김은정
    • 한국패류학회지
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    • 제14권2호
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    • pp.149-159
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    • 1998

  • In order to observe the anticellulolytic localization in the epithelia of the digestive tract such as esophagus, crop, and intestine of a Korean land snail N. samarangae, a cytochemical method and a immunogold labelling method were applied. For the cytochemical study on the cellulase activity, Benedict reaction method applied. And for the immunocytochemical study, the rabbit serum immunoglobuins (IgG) was obtained from the rabbits injected with cellulase which was extracted from body fluid of the snail. The digestive tract tissues of N. samarangae were fixed with 4% paraformaldehyde and 2% OsO4 and embedded in Lowicryl K4M at -40$^{\circ}C$ under UV light (360 nm). The thin sections were loaded on the nickel grids and stained with the serum IgG and protein A-gold complex (particle size: 10 nm). Observations were undertaken with transmission electron microscope (Jeol, JEM-1010). The epithelium of the digestive tract was consisted of five types of cells. In the cytochemical study, the reaction products were found along the periphery of the vacuoles derived from the Bebedict reaction. In the immunocytochemical study, the protein-A gold particles were selectively labelled in Type 1, Type 3 and Type 4 cells in intestinal tissue. membranes of rER, in the surrounding cytoplasm of the rER and secretory granules, and in the apical cytoplasm of the cells. On the material being secreted from the apical cytoplasm was also labelled with the immunogold particles. The all results obtained throughtout present study suggest that the intestinal epithelium of the snail N. samarangae seretes cellulase as one of digestive enzymes.

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동양달팽이의 소화관에 대한 조직화학적 및 미세구조적 연구 (Histochemical and Ultrastructural Study on the Digestive Tract of a Land Snail Nesiohelix samarangae)

  • 정계헌;이용석
    • 한국패류학회지
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    • 제14권2호
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    • pp.131-147
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    • 1998

  • A histochemical and ultrastructural study on the epithelia of some selected digestive tracks such as esophagus, crop, intestine of a land snail N. samarangae was carried out during the period of June 1997 to may 1998. The epithelium of digestive tract are simple columnar epithelium and consisted of five types of columnar cells. Type 1 cell which is majority in number has a brush border with microvilli on the free surface of the cell and contains numerous secretory granules supposed to be neutral mucopolysaccharide. Type 2 cell, elongated conical in shape, is rarely found in the epithelium. This cell also has a brush border with microvilli on its free surface and contains well developed rough surfaced endoplasmic reticulum, Golgi bodies, and secretory granules in various electron densities. This cell seems to produce both of acid and neutral mucopolysaccharides. Type 3 cell, which is morphologically similar to the Type 1 cell, has microvilli and cilia on the free surface and exists in group only in the limited regions of the intestine. Type 4 cell, typical goblet cell containing secretory granules in high electron density. Type 5 cell rarely found in the digestive tract. This cell contain inconspicuous materials.

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Cytochemical and Immunocytochemical Study on the Cellulase Activity in the Accesssory Glands of the Digestive System of the Oriental Land Snail, Nesiohelix samarangae

  • Jeong, Kye-Heon;Lee, Yong-Seok;Shim, Yun-Bo
    • 한국어업기술학회:학술대회논문집
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    • 한국어업기술학회 2000년도 춘계수산관련학회 공동학술대회발표요지집
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    • pp.538-538
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    • 2000

  • Histochemical, cytochemical, and immmunocytochemical investigations were conducted to find out the cellulase activities in the accessory glands of the digestive system in the oriental land snail Nesiohelix samarangae under the LM, SEM, and TEM. It was found that the Type 1 and Type 3 cells out of five types of the epithelial cells of the digestive gland were labeled with the immunogold(protein-A gold) particles. Otherwise, none of epithelial cells of the mucus gland and the salivary gland were not labeled with the immunogold particles.

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DNA Barcoding of Aegista chejuensis and Plectotropis quelpartensis (Gastropoda: Stylommatophora: Camaenidae)

  • Kang-San Kim;Jun-Sang Lee
    • Animal Systematics, Evolution and Diversity
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    • 제39권4호
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    • pp.295-299
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    • 2023

  • Two land snails, Aegista chejuensis (Pilsbry and Hirase, 1908) and Plectotropis quelpartensis (Pilsbry and Hirase, 1908), are endemic to Korea and were collected from Hataedo and Jodo Islands in the Yellow Sea of South Korea, respectively. Many terrestrial snail habitats have been confirmed in Korea; however, their genetic sequences have rarely been reported. This study describes the mitochondrial cytochrome c oxidase subunit I gene (COI) sequences of two species, followed by an analysis of the genetic distance between these two species and their congeners. As a result, there was no intra-species variation in both species A. chejuensis or P. quelpartensis. However, the inter-species variation was clear (10.3-31.5%). We provide photographs and a brief diagnosis for morphological verification.

  • https://doi.org/10.5635/ASED.2023.39.4.022 인용 PDF